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Open AccessArticle

Low Replication Efficiency of a Japanese Rabbit Hepatitis E Virus Strain in the Human Hepatocarcinoma Cell Line PLC/PRF/5

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Wenjing Zhang

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Milagros Virhuez Mendoza

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Yasushi Ami

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Yuriko Suzaki

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Yen Hai Doan

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Ken Maeda

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Tiancheng Li

Viruses 2023, 15(6), 1322; https://doi.org/10.3390/v15061322 (registering DOI) - 05 Jun 2023

Abstract

A Japanese rabbit hepatitis E virus (HEV) strain, JP-59, has been identified in a feral rabbit. When this virus was transmitted to a Japanese white rabbit, it caused persistent HEV infection. The JP-59 strain shares an <87.5% nucleotide sequence identity with other rabbit [...] Read more.

A Japanese rabbit hepatitis E virus (HEV) strain, JP-59, has been identified in a feral rabbit. When this virus was transmitted to a Japanese white rabbit, it caused persistent HEV infection. The JP-59 strain shares an <87.5% nucleotide sequence identity with other rabbit HEV strains. Herein, to isolate JP-59 by cell culture, we used a 10% stool suspension recovered from a JP-59-infected Japanese white rabbit and contained 1.1 × 10⁷ copies/mL of the viral RNA and using it to infect a human hepatocarcinoma cell line, PLC/PRF/5. No sign of virus replication was observed. Although long-term virus replication was observed in PLC/PRF/5 cells inoculated with the concentrated and purified JP-59 containing a high titer of viral RNA (5.1 × 10⁸ copies/mL), the viral RNA of JP-59c that was recovered from the cell culture supernatants was <7.1 × 10⁴ copies/mL during the experiment. The JP-59c strain did not infect PLC/PRF/5 cells, but its intravenous inoculation caused persistent infection in rabbits. The nucleotide sequence analyses of the virus genomes demonstrated that a total of 18 nucleotide changes accompanying three amino acid mutations occurred in the strain JP-59c compared to the original strain JP-59. These results indicate that a high viral RNA titer was required for JP-59 to infect PLC/PRF/5 cells, but its replication capability was extremely low. In addition, the ability of rabbit HEVs to multiply in PLC/PRF/5 cells varied depending on the rabbit HEV strains. The investigations of cell lines that are broadly susceptible to rabbit HEV and that allow the efficient propagation of the virus are thus needed. Full article

(This article belongs to the Section Animal Viruses)

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Open AccessArticle

Design and Development of 3D-Printed Personalized Femoral Prosthesis Technologies

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Coatings 2023, 13(6), 1044; https://doi.org/10.3390/coatings13061044 (registering DOI) - 05 Jun 2023

Abstract

The femur supports the entire body weight, and any damage or necrosis to this bone can significantly impair normal walking. Therefore, repairing the femur is essential to restoring its function. However, due to variations in human bone structure, standardized prostheses often deliver poor [...] Read more.

The femur supports the entire body weight, and any damage or necrosis to this bone can significantly impair normal walking. Therefore, repairing the femur is essential to restoring its function. However, due to variations in human bone structure, standardized prostheses often deliver poor repair outcomes. We used the medical three-dimensional (3D) auxiliary software Mimics to design personalized femoral prostheses to address this issue. The femoral prosthesis filler was porous, and all aspects of the prosthesis were thoroughly studied and analyzed before direct molding using 3D printing technology. The personalized femoral prosthesis filler and bone plate designed using 3D printing technology have positive effects, and the matching between the femoral prosthesis, bone plate, and filler is satisfactory. The pore structure of the 3D-printed femoral prosthesis filler and the bone plate is clear and of high quality, which shortens the research and development cycle and reduces costs, providing a foundation for the direct application of 3D-printed personalized prostheses. Full article

(This article belongs to the Special Issue Recent Development in Post-processing for Additive Manufacturing)

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Open AccessArticle

Baptismal Aesthetics In-between: Reflections on the Interplay of Text, Rite, and Image in the Sanctuaries of Ravenna

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Isabella Bruckner

Religions 2023, 14(6), 743; https://doi.org/10.3390/rel14060743 (registering DOI) - 05 Jun 2023

Abstract

Baptism is the sacramental celebration of Christian initiation. Paul’s letter to the Romans, which is central to the understanding of baptism, characterizes this sacramental event as a dying with Christ and the beginning of a new existence. This new mode of existence gains [...] Read more.

Baptism is the sacramental celebration of Christian initiation. Paul’s letter to the Romans, which is central to the understanding of baptism, characterizes this sacramental event as a dying with Christ and the beginning of a new existence. This new mode of existence gains an aesthetic-performative form in the liturgical rites. The design of the liturgical spaces can then be understood as “petrified rites”. The imperial church basilicas and baptisteries of the Byzantine period in Ravenna bear particular witness to such petrified manifestations of liturgy. What took place in the liturgical rites found an aesthetic counterpart in the interior design and in the rich mosaic art of the ancient buildings. The Ravennese color-intensive wall and ceiling motifs substantiate in a sensuous way the eschatological aesthetic, which is opened to believers through baptism. Biblical texts, architecture, rite, and pictorial program thus form an aesthetic ensemble whose elements mutually illuminate each other and only gain their full depth of meaning in the context of this performative dynamic. This contribution analyzes the interplay of these different registers, based on some selected examples of Ravenna’s sacred buildings, and explores how the baptismal event is conveyed in them as an aesthetic access to the world. Full article

(This article belongs to the Special Issue Theology and Aesthetics)

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Open AccessArticle

Grape Seed Extract as a Feed Additive Improves the Growth Performance, Ruminal Fermentation and Immunity of Weaned Beef Calves

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Animals 2023, 13(11), 1876; https://doi.org/10.3390/ani13111876 (registering DOI) - 05 Jun 2023

Abstract

The purpose of this research was to evaluate effects of grape seed extract (Gse) supplementation on the growth performance; ruminal fermentation; nutrient digestibility; and serum biochemical, antioxidative, and immune parameters of weaned beef calves. A total of 30 Simmental crossbred male calves with [...] Read more.

The purpose of this research was to evaluate effects of grape seed extract (Gse) supplementation on the growth performance; ruminal fermentation; nutrient digestibility; and serum biochemical, antioxidative, and immune parameters of weaned beef calves. A total of 30 Simmental crossbred male calves with similar age and body weight were randomly allocated to two groups: a control group with no Gse (CON) and a Gse supplementation group (GSE) (4 g/d Gse per animal). The results show that, compared with the CON group, the average daily gain significantly increased (p = 0.043) in the GSE group. The ruminal contents of microbial protein and butyrate in GSE group were higher (p < 0.05) than those in the CON group. Additionally, calves fed Gse displayed increased (p < 0.05) dry matter and neutral detergent fiber digestibility. Moreover, the serum concentrations of triglyceride, catalase, superoxide dismutase, immunoglobulin G and immunoglobulin M were higher (p < 0.05) in the GSE group than those in the CON group. However, opposite tendencies of non-esterified fatty acid, malondialdehyde, tumor necrosis factor-α and interleukin-6 were found between the two groups. Overall, the supplementation of Gse can improve ruminal fermentation, nutrient digestibility, antioxidant ability, and immunity, as well as promoting the healthy growth of weaned cross-breed beef calves. Full article

(This article belongs to the Section Cattle)

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Open AccessArticle

Evaluation of Neutrophil-to-Lymphocyte Ratio (NLR), Platelet-to-Lymphocyte Ratio (PLR) and Systemic Immune–Inflammation Index (SII) as Potential Biomarkers in Patients with Sporadic Medullary Thyroid Cancer (MTC)

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J. Pers. Med. 2023, 13(6), 953; https://doi.org/10.3390/jpm13060953 (registering DOI) - 05 Jun 2023

Abstract

Medullary thyroid cancer (MTC) is a rare neuroendocrine neoplasm, and calcitonin is its main biomarker. An elevated neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune–inflammation index (SII) have been considered as negative prognostic factors in several neoplasms. The aim of this study [...] Read more.

Medullary thyroid cancer (MTC) is a rare neuroendocrine neoplasm, and calcitonin is its main biomarker. An elevated neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune–inflammation index (SII) have been considered as negative prognostic factors in several neoplasms. The aim of this study is to evaluate the potential role of NLR, PLR and SII as biomarkers in MTC. Clinical data and tumor histological characteristics of patients with sporadic MTC, referred to the NET Unit of Federico II University of Naples (ENETS CoE) from 2012 to 2022, were retrospectively evaluated by analyzing preoperative and postoperative calcitonin, NLR, PLR and SII. We included 35 MTC patients undergoing total thyroidectomy. The mean preoperative NLR was 2.70 (±1.41, 0.93–7.98), the PLR was 121.05 (±41.9, 40.98–227.23) and SII was 597.92 (±345.58, 186.59–1628). We identified a statistically significant difference between pre- and post-thyroidectomy NLR (p = 0.02), SII (p = 0.02) and calcitonin (p = 0.0) values. No association with prognosis or tumor characteristics emerged. Elevated preoperative NLR and SII suggest a possible disease-associated inflammatory response, and their reduction after surgery may be related to debulking effects. Further studies are needed to define the role of NLR, PLR and SII as prognostic markers in MTC. Full article

(This article belongs to the Special Issue Cancer Biomarker Research and Personalized Medicine 2.0)

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Open AccessArticle

Synergistic Effects of Kaolin and Silicon Nanoparticles for Ameliorating Deficit Irrigation Stress in Maize Plants by Upregulating Antioxidant Defense Systems

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Alshymaa Z. Al-Mokadem

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Mohamed H. Sheta

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Ahmed G. Mancy

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Hebat-Allah A. Hussein

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Sahar K. M. Kenawy

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Ahmed R. Sofy

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Mahmoud S. Abu-Shahba

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Plants 2023, 12(11), 2221; https://doi.org/10.3390/plants12112221 (registering DOI) - 05 Jun 2023

Abstract

Water deficit is a significant environmental stress that has a negative impact on plant growth and yield. In this research, the positive significance of kaolin and SiO₂ nanoparticles in moderating the detrimental effects of water deficit on maize plant growth and yield [...] Read more.

Water deficit is a significant environmental stress that has a negative impact on plant growth and yield. In this research, the positive significance of kaolin and SiO₂ nanoparticles in moderating the detrimental effects of water deficit on maize plant growth and yield is investigated. The foliar application of kaolin (3 and 6%) and SiO₂ NPs (1.5 and 3 mM) solutions increased the growth and yield variables of maize plants grown under normal conditions (100% available water) and drought stress conditions (80 and 60% available water (AW)). In addition, plants treated with SiO₂ NPs (3 mM) demonstrated increased levels of important osmolytes, such as proline and phenol, and maintained more of their photosynthetic pigments (net photosynthetic rate (PN), stomatal conductance (gs), intercellular CO₂ concentration (Ci), and transpiration rate (E)) than with other applied treatments under either stress or non-stress conditions. Furthermore, the exogenous foliar application of kaolin and SiO₂ NPs also reduced the amounts of hydroxyl radicals (OH), superoxide anions (O₂), hydrogen peroxide (H₂O₂), and lipid peroxidation in maize plants experiencing a water deficit. In contrast, the treatments led to an increase in the activity of antioxidant enzymes such as peroxidase (POX), ascorbate peroxidase (APX), glutathione peroxidase (GR), catalase (CAT), and superoxide dismutase (SOD). Overall, our findings indicate the beneficial impact of the application of kaolin and silicon NPs, particularly the impact of SiO₂ NPs (3 mM) on managing the negative, harmful impacts of soil water deficit stress in maize plants. Full article

(This article belongs to the Special Issue The Impacts of Abiotic Stresses on Plant Development 2.0)

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Open AccessArticle

Assessment of Nursing Students’ Awareness toward Ionizing Radiation: Cross-Sectional Study

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Nurs. Rep. 2023, 13(2), 855-864; https://doi.org/10.3390/nursrep13020075 (registering DOI) - 05 Jun 2023

Abstract

Among healthcare workers, nurses are considered the core of healthcare auth–info services in healthcare facilities because of their responsibilities and duties toward patients. All healthcare professionals, especially nurses, must be completely knowledgeable about the hazards of ionizing radiation, and the most effective radiation [...] Read more.

Among healthcare workers, nurses are considered the core of healthcare auth–info services in healthcare facilities because of their responsibilities and duties toward patients. All healthcare professionals, especially nurses, must be completely knowledgeable about the hazards of ionizing radiation, and the most effective radiation protection techniques. This study assessed the attitude and awareness toward radiation protection among final-year nursing students in the Fatima College of Health Sciences (FCHS) campuses. An online cross-sectional survey was conducted between March and April 2022. A total of 200 out of 224 female participants ranging from 18 to 30 years old agreed to participate in the study. The highest percentage of final-year nursing students did not attend any radiation protection course (52%). The results of the last section of the survey indicate a lack of awareness of basic radiation protection knowledge among final-year nursing students in FCHS campuses (less than 80%). The results showed a lack of knowledge and poor attitude toward radiation hazards and radiation protection from final-year nursing students in the FCHS. Formal education about basic radiation and radiation is recommended in the nursing program for safe clinical practice. Full article

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Open AccessArticle

Transformation of Paddy Field Use in Intermountain-Type Basins Using Evidence from the Structure and Function Perspective of Karst Mountain Areas in Southwest China

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Agronomy 2023, 13(6), 1552; https://doi.org/10.3390/agronomy13061552 (registering DOI) - 05 Jun 2023

Abstract

China’s southwestern karst area exhibits many mountains and little flat lands, and intermountain basins (IBs) (locally named “bazi”) are one of their typical landform types. Comparative studies on land use in the core of typical landforms in karst mountain areas are relatively lacking. [...] Read more.

China’s southwestern karst area exhibits many mountains and little flat lands, and intermountain basins (IBs) (locally named “bazi”) are one of their typical landform types. Comparative studies on land use in the core of typical landforms in karst mountain areas are relatively lacking. Studying the evolution and transformation patterns of intermountain basin paddy fields use (IBPFU) to optimize land resources in mountainous areas is important. Based on structural and functional perspectives, this study established a research framework on the evolution and transformation of IBPFU in karst mountainous areas, and analyzed the evolution and transformation patterns of IBPFU in Guizhou Province from 1966 to 2020 by measuring land use change and applying the moving window method and morphological spatial pattern analysis (MSPA) model. The study showed that the evolution of IBPFU is characterized by reversibility and irreversibility, diversity, and stages. The transition of IBPFU requires a trade-off among regional socioeconomic development, ecological protection, and food security. The commonality of land use transitions in IB and sloped land (SL) is reflected in the diversity of their land use functions. This study provides a reference for a comprehensive understanding of land use transitions and sustainable development in the mountainous karst regions of southwest China by providing in-depth examinations of the land use transition of IB, which is different from SL, via the long time series evolution of core land use types. The research framework and research method constructed here also apply to other regions. Full article

(This article belongs to the Special Issue Crop Productivity and Energy Balance in Large-Scale Fields II)

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Open AccessEditorial

Total Hip Arthroplasty: So Hip It Hurts

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Lukas A. Holzer

J. Clin. Med. 2023, 12(11), 3849; https://doi.org/10.3390/jcm12113849 (registering DOI) - 05 Jun 2023

Abstract

Total hip arthroplasty (THA) has become a standard surgical intervention for patients with hip joint disorders [...] Full article

(This article belongs to the Special Issue Hip Surgery: Clinical Treatment and Management)

Open AccessPerspective

Polymers and Bioactive Compounds with a Macrophage Modulation Effect for the Rational Design of Hydrogels for Skin Regeneration

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Mirna L. Sánchez

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Hugo Valdez

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Micaela Conde

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Pamela Viaña-Mendieta

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Aldo R. Boccaccini

Pharmaceutics 2023, 15(6), 1655; https://doi.org/10.3390/pharmaceutics15061655 (registering DOI) - 05 Jun 2023

Abstract

The development of biomaterial platforms for dispensing reagents of interest such as antioxidants, growth factors or antibiotics based on functional hydrogels represents a biotechnological solution for many challenges that the biomedicine field is facing. In this context, in situ dosing of therapeutic components [...] Read more.

The development of biomaterial platforms for dispensing reagents of interest such as antioxidants, growth factors or antibiotics based on functional hydrogels represents a biotechnological solution for many challenges that the biomedicine field is facing. In this context, in situ dosing of therapeutic components for dermatological injuries such as diabetic foot ulcers is a relatively novel strategy to improve the wound healing process. Hydrogels have shown more comfort for the treatment of wounds due to their smooth surface and moisture, as well as their structural affinity with tissues in comparison to hyperbaric oxygen therapy, ultrasound, and electromagnetic therapies, negative pressure wound therapy or skin grafts. Macrophages, one of the most important cells of the innate immune system, have been described as the key not only in relation to the host immune defense, but also in the progress of wound healing. Macrophage dysfunction in chronic wounds of diabetic patients leads to a perpetuating inflammatory environment and impairs tissue repair. Modulating the macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) could be a strategy for helping to improve chronic wound healing. In this regard, a new paradigm is found in the development of advanced biomaterials capable of inducing in situ macrophage polarization to offer an approach to wound care. Such an approach opens a new direction for the development of multifunctional materials in regenerative medicine. This paper surveys emerging hydrogel materials and bioactive compounds being investigated to induce the immunomodulation of macrophages. We propose four potential functional biomaterials for wound healing applications based on novel biomaterial/bioactive compound combination that are expected to show synergistic beneficial outcomes for the local differentiation of macrophages (M1–M2) as a therapeutic strategy for chronic wound healing improvement. Full article

(This article belongs to the Special Issue Biopolymer Materials for Wound Healing (Volume II))

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Open AccessArticle

Research and Evaluation on Dynamic Maintenance of an Elevation Datum Based on CORS Network Deformation

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Remote Sens. 2023, 15(11), 2935; https://doi.org/10.3390/rs15112935 (registering DOI) - 05 Jun 2023

Abstract

This paper presents a method for dynamically maintaining a regional elevation datum using CORS stations as core nodes. By utilizing CORS station data and surface mass loading data (including land water storage, sea level, and atmospheric pressure), the normal height changes of each [...] Read more.

This paper presents a method for dynamically maintaining a regional elevation datum using CORS stations as core nodes. By utilizing CORS station data and surface mass loading data (including land water storage, sea level, and atmospheric pressure), the normal height changes of each station can be determined and dynamically maintained. The validity of this method is verified using multiple leveling survey results from five CORS stations in Beijing’s subsidence area between January 2012 and June 2021. Results show that it is necessary to derive and correct the height anomaly variation of CORS stations caused by surface mass loading using the remove-calculate-restore method and the Green’s function integration method, with the influence of surface mass changes reaching a subcentimeter level. CORS stations exhibiting great observation quality achieve a mean accuracy of 2.7 mm in determining normal height changes. Such accuracy surpasses the requirements of second-class leveling surveys covering route lengths exceeding 1.35 km, as well as conforming/closed loop routes with distances greater than 0.46 km. By strategically selecting CORS stations with long-term continuous observations and high-quality data as core nodes within the elevation control network, dynamic maintenance of the regional elevation datum can be achieved based on CORS station data. Full article

(This article belongs to the Topic Development of Monitoring, Analysis and Maintenance Technics of Infrastructures)

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Open AccessArticle

Characterization of Chromatin Accessibility in Fetal Bovine Chondrocytes

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Animals 2023, 13(11), 1875; https://doi.org/10.3390/ani13111875 (registering DOI) - 05 Jun 2023

Abstract

Despite significant advances of the bovine epigenome investigation, new evidence for the epigenetic basis of fetal cartilage development remains lacking. In this study, the chondrocytes were isolated from long bone tissues of bovine fetuses at 90 days. The Assay for Transposase-Accessible Chromatin with [...] Read more.

Despite significant advances of the bovine epigenome investigation, new evidence for the epigenetic basis of fetal cartilage development remains lacking. In this study, the chondrocytes were isolated from long bone tissues of bovine fetuses at 90 days. The Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) and transcriptome sequencing (RNA-seq) were used to characterize gene expression and chromatin accessibility profile in bovine chondrocytes. A total of 9686 open chromatin regions in bovine fetal chondrocytes were identified and 45% of the peaks were enriched in the promoter regions. Then, all peaks were annotated to the nearest gene for Gene Ontology (GO) and Kyoto Encylopaedia of Genes and Genomes (KEGG) analysis. Growth and development-related processes such as amide biosynthesis process (GO: 0043604) and translation regulation (GO: 006417) were enriched in the GO analysis. The KEGG analysis enriched endoplasmic reticulum protein processing signal pathway, TGF-β signaling pathway and cell cycle pathway, which are closely related to protein synthesis and processing during cell proliferation. Active transcription factors (TFs) were enriched by ATAC-seq, and were fully verified with gene expression levels obtained by RNA-seq. Among the top50 TFs from footprint analysis, known or potential cartilage development-related transcription factors FOS, FOSL2 and NFY were found. Overall, our data provide a theoretical basis for further determining the regulatory mechanism of cartilage development in bovine. Full article

(This article belongs to the Section Cattle)

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Open AccessArticle

Combined Use of Ultrasonic and Electromagnetic Fields for the Study of Bonding Mechanisms between Dexamethasone Disodium Phosphate Molecules

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Constantine Kouderis

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Angelos G. Kalampounias

Quantum Beam Sci. 2023, 7(2), 19; https://doi.org/10.3390/qubs7020019 (registering DOI) - 05 Jun 2023

Abstract

We have investigated the ultrasonically induced birefringence traces of aqueous solutions of dexamethasone disodium phosphate, a derivative of hydrocortisone (cortisol). The stationary birefringence and the transient built-up and decay relaxation processes were studied as a function of solution concentration, ultrasound frequency and intensity, [...] Read more.

We have investigated the ultrasonically induced birefringence traces of aqueous solutions of dexamethasone disodium phosphate, a derivative of hydrocortisone (cortisol). The stationary birefringence and the transient built-up and decay relaxation processes were studied as a function of solution concentration, ultrasound frequency and intensity, as well as a function of temperature. The results were analyzed in view of structural peculiarities of the system in an effort to gain further insights into the molecular relaxation dynamics and the proposed self-association process occurring in the system. The detected ultrasonically induced birefringence relaxation is motivated by the rotational diffusion of dexamethasone disodium phosphate aggregates due to self-association depending on the solution concentration. The observed relaxation mechanism is directly linked to the hydrodynamic size of the acoustic field-induced self-assembly. The systematic analysis of the transient birefringence signals caused by the applied ultrasonic field allowed us to evaluate the interplay between permanent and induced dipoles with changing concentration, temperature, and ultrasound properties. The birefringence traces are adequately fitted with a stretched exponential law indicating the polydispersive nature of the self-aggregated molecular structures. The obtained results are described in the light of recent studies performed on this system. Full article

(This article belongs to the Section Spectroscopy Technique)

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Open AccessReview

Virophages—Known and Unknown Facts

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Viruses 2023, 15(6), 1321; https://doi.org/10.3390/v15061321 (registering DOI) - 05 Jun 2023

Abstract

The paper presents virophages, which, like their host, giant viruses, are “new” infectious agents whose role in nature, including mammalian health, is important. Virophages, along with their protozoan and algal hosts, are found in fresh inland waters and oceanic and marine waters, including [...] Read more.

The paper presents virophages, which, like their host, giant viruses, are “new” infectious agents whose role in nature, including mammalian health, is important. Virophages, along with their protozoan and algal hosts, are found in fresh inland waters and oceanic and marine waters, including thermal waters and deep-sea vents, as well as in soil, plants, and in humans and animals (ruminants). Representing “superparasitism”, almost all of the 39 described virophages (except Zamilon) interact negatively with giant viruses by affecting their replication and morphogenesis and their “adaptive immunity”. This causes them to become regulators and, at the same time, defenders of the host of giant viruses protozoa and algae, which are organisms that determine the homeostasis of the aquatic environment. They are classified in the family Lavidaviridae with two genus (Sputnikovirus, Mavirus). However, in 2023, a proposal was presented that they should form the class Maveriviricetes, with four orders and seven families. Their specific structure, including their microsatellite (SSR-Simple Sequence Repeats) and the CVV (cell—virus—virophage, or transpovirion) system described with them, as well as their function, makes them, together with the biological features of giant viruses, form the basis for discussing the existence of a fourth domain in addition to Bacteria, Archaea, and Eukaryota. The paper also presents the hypothetical possibility of using them as a vector for vaccine antigens. Full article

(This article belongs to the Section General Virology)

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Open AccessArticle

Changes in Surface Hydrophobicity of Coal Particles and the Formation of Coarse Particle–Bubble Clusters in the Process of High-Intensity Conditioning

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Processes 2023, 11(6), 1723; https://doi.org/10.3390/pr11061723 (registering DOI) - 05 Jun 2023

Abstract

The mechanism of high-intensity conditioning (HIC) has not been thoroughly revealed, and therefore this work investigates the effect of HIC on the surface hydrophobicity of coal with different particle sizes and the possible formation of particle–bubble clusters. The results show that different HIC [...] Read more.

The mechanism of high-intensity conditioning (HIC) has not been thoroughly revealed, and therefore this work investigates the effect of HIC on the surface hydrophobicity of coal with different particle sizes and the possible formation of particle–bubble clusters. The results show that different HIC conditions are required for coarse and fine particles. Coarse particles (+75 μm) require a higher turbulence intensity to increase collector dispersion, thereby increasing the adsorption of the collector. Fine particles (−75 μm) require a lower turbulence intensity to reduce the desorption of the collector. In this study, the optimum HIC conditions for coarse and fine particles are “2200 rpm + 1 min” and “1300 rpm + 1 min”, respectively. Interestingly, it seems that the adsorption capacity between fine particles and the collector is weaker than that for coarse particles. A non-enclosed HIC system produces up to 1.78 × 10⁴/g bubbles in coarse particle–bubble clusters, and the mean bubble diameter is approximately 87 μm. The cluster achieves pre-mineralization and increases the apparent particle size, which is expected to improve flotation. Full article

(This article belongs to the Special Issue Process Analysis and Carbon Emission of Mineral Separation Processes)

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Open AccessArticle

Screening Biocontrol Agents for Cash Crop Fusarium Wilt Based on Fusaric Acid Tolerance and Antagonistic Activity against Fusarium oxysporum

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Toxins 2023, 15(6), 381; https://doi.org/10.3390/toxins15060381 (registering DOI) - 05 Jun 2023

Abstract

Fusarium wilt, caused by Fusarium oxysporum, is one of the most notorious diseases of cash crops. The use of microbial fungicides is an effective measure for controlling Fusarium wilt, and the genus Bacillus is an important resource for the development of microbial [...] Read more.

Fusarium wilt, caused by Fusarium oxysporum, is one of the most notorious diseases of cash crops. The use of microbial fungicides is an effective measure for controlling Fusarium wilt, and the genus Bacillus is an important resource for the development of microbial fungicides. Fusaric acid (FA) produced by F. oxysporum can inhibit the growth of Bacillus, thus affecting the control efficacy of microbial fungicides. Therefore, screening FA-tolerant biocontrol Bacillus may help to improve the biocontrol effect on Fusarium wilt. In this study, a method for screening biocontrol agents against Fusarium wilt was established based on tolerance to FA and antagonism against F. oxysporum. Three promising biocontrol bacteria, named B31, F68, and 30833, were obtained to successfully control tomato, watermelon, and cucumber Fusarium wilt. Strains B31, F68, and 30833 were identified as B. velezensis by phylogenetic analysis of the 16S rDNA, gyrB, rpoB, and rpoC gene sequences. Coculture assays revealed that strains B31, F68, and 30833 showed increased tolerance to F. oxysporum and its metabolites compared with B. velezensis strain FZB42. Further experiments confirmed that 10 µg/mL FA completely inhibited the growth of strain FZB42, while strains B31, F68, and 30833 maintained normal growth at 20 µg/mL FA and partial growth at 40 µg/mL FA. Compared with strain FZB42, strains B31, F68, and 30833 exhibited significantly greater tolerance to FA. Full article

(This article belongs to the Special Issue Detoxification, Control, and Toxicological Studies on Mycotoxins through Biotechnological Approaches)

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Open AccessArticle

Research on Rolling Bearing Fault Diagnosis Based on Digital Twin Data and Improved ConvNext

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Sensors 2023, 23(11), 5334; https://doi.org/10.3390/s23115334 (registering DOI) - 05 Jun 2023

Abstract

This article introduces a novel framework for diagnosing faults in rolling bearings. The framework combines digital twin data, transfer learning theory, and an enhanced ConvNext deep learning network model. Its purpose is to address the challenges posed by the limited actual fault data [...] Read more.

This article introduces a novel framework for diagnosing faults in rolling bearings. The framework combines digital twin data, transfer learning theory, and an enhanced ConvNext deep learning network model. Its purpose is to address the challenges posed by the limited actual fault data density and inadequate result accuracy in existing research on the detection of rolling bearing faults in rotating mechanical equipment. To begin with, the operational rolling bearing is represented in the digital realm through the utilization of a digital twin model. The simulation data produced by this twin model replace traditional experimental data, effectively creating a substantial volume of well-balanced simulated datasets. Next, improvements are made to the ConvNext network by incorporating an unparameterized attention module called the Similarity Attention Module (SimAM) and an efficient channel attention feature referred to as the Efficient Channel Attention Network (ECA). These enhancements serve to augment the network’s capability for extracting features. Subsequently, the enhanced network model is trained using the source domain dataset. Simultaneously, the trained model is transferred to the target domain bearing using transfer learning techniques. This transfer learning process enables the accurate fault diagnosis of the main bearing to be achieved. Finally, the proposed method’s feasibility is validated, and a comparative analysis is conducted in comparison with similar approaches. The comparative study demonstrates that the proposed method effectively addresses the issue of low mechanical equipment fault data density, leading to improved accuracy in fault detection and classification, along with a certain level of robustness. Full article

(This article belongs to the Special Issue Sensors for Fault Detection and Condition Monitoring)

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Open AccessReview

5′-Substituted Indoline Spiropyrans: Synthesis and Applications

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Valery A. Barachevsky

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Sergey D. Varfolomeev

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Olga V. Demina

Colorants 2023, 2(2), 264-404; https://doi.org/10.3390/colorants2020017 (registering DOI) - 05 Jun 2023

Abstract

Methods for preparation of 5′-substituted spiropyrans, their chemical properties, and the effects of various factors on the relative stabilities of the spiropyrans and their isomeric merocyanine forms are examined, reviewed, and discussed. Full article

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Open AccessArticle

Attitudes towards Urban Air Mobility for E-Commerce Deliveries: An Exploratory Survey Comparing European Regions

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André Teixeira Silva

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Sérgio Pedro Duarte

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Sandra Melo

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Adriana Witkowska-Konieczny

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Michele Giannuzzi

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António Lobo

Aerospace 2023, 10(6), 536; https://doi.org/10.3390/aerospace10060536 (registering DOI) - 05 Jun 2023

Abstract

This study explores attitudes towards urban air mobility (UAM) for e-commerce deliveries. UAM, which utilizes drones, has the potential to revolutionize transport services and logistics, leading to economic benefits and reductions in congestion and pollution. However, public acceptance is crucial for a successful [...] Read more.

This study explores attitudes towards urban air mobility (UAM) for e-commerce deliveries. UAM, which utilizes drones, has the potential to revolutionize transport services and logistics, leading to economic benefits and reductions in congestion and pollution. However, public acceptance is crucial for a successful implementation; thus, understanding the people’s perspective is key. Descriptive statistics were employed to evaluate survey results from three different European regions, followed by a cluster analysis to define potential user profiles. This study revealed slightly different perceptions towards UAM between the analyzed regions, but also a generally positive attitude. The most important expected identified benefits from UAM were a decrease in congestion and pollution in city centers. High-acceptance segments are gender-balanced and correspond mostly to active population and frequent online shoppers that perceive value in drone deliveries, especially related to an increased convenience and speed. They support public investment in UAM and are willing to pay more for these services and to have their home flown over. Opposite attitudes were expressed by low-acceptance segments, which are female-dominated and include mostly non-frequent online shoppers. The identified user profiles in this study can support the development of public policy and marketing strategies to increase acceptance and adoption. Full article

(This article belongs to the Special Issue Advanced Air Mobility)

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Open AccessEditorial

Electromagnetic Preparation of Materials: From Fundamentals to Applications

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Chuanjun Li

and

Sheng Yu

Metals 2023, 13(6), 1073; https://doi.org/10.3390/met13061073 (registering DOI) - 05 Jun 2023

Abstract

Magnetic phenomena are amazing and mysterious topics [...] Full article

(This article belongs to the Special Issue Electromagnetic Preparation of Metallic Materials)

Open AccessReview

A Review of Recent Advances for the Detection of Biological, Chemical and Physical Hazards in Foodstuffs Using Spectral Imaging Techniques

by

Chuanqi Xie

and

Weidong Zhou

Foods 2023, 12(11), 2266; https://doi.org/10.3390/foods12112266 (registering DOI) - 05 Jun 2023

Abstract

Traditional methods for detecting foodstuff hazards are time-consuming, inefficient, and destructive. Spectral imaging techniques have been proven to overcome these disadvantages in detecting foodstuff hazards. Compared with traditional methods, spectral imaging could also increase the throughput and frequency of detection. This study reviewed [...] Read more.

Traditional methods for detecting foodstuff hazards are time-consuming, inefficient, and destructive. Spectral imaging techniques have been proven to overcome these disadvantages in detecting foodstuff hazards. Compared with traditional methods, spectral imaging could also increase the throughput and frequency of detection. This study reviewed the techniques used to detect biological, chemical, and physical hazards in foodstuffs including ultraviolet, visible and near-infrared (UV-Vis-NIR) spectroscopy, terahertz (THz) spectroscopy, hyperspectral imaging, and Raman spectroscopy. The advantages and disadvantages of these techniques were discussed and compared. The latest studies regarding machine learning algorithms for detecting foodstuff hazards were also summarized. It can be found that spectral imaging techniques are useful in the detection of foodstuff hazards. Thus, this review provides updated information regarding the spectral imaging techniques that can be used by food industries and as a foundation for further studies. Full article

(This article belongs to the Section Food Analytical Methods)

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Open AccessArticle

Towards the Voluntary Adoption of Integrated Reporting: Drivers, Barriers, and Practices

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Adm. Sci. 2023, 13(6), 148; https://doi.org/10.3390/admsci13060148 (registering DOI) - 05 Jun 2023

Abstract

Integrated reporting is essentially a voluntary practice worldwide. For this reason, although it is growing, its diffusion is slow. Based on the Portuguese context, where a reduced number of listed companies publish integrated reports, this study aims to explore the drivers and barriers [...] Read more.

Integrated reporting is essentially a voluntary practice worldwide. For this reason, although it is growing, its diffusion is slow. Based on the Portuguese context, where a reduced number of listed companies publish integrated reports, this study aims to explore the drivers and barriers for the voluntary adoption of integrated reporting and the reporting practices up to the production of an integrated report. To this end, an analytical framework based on the Diffusion of Innovation Theory was developed and applied to data collected from a survey and corporate reports from both “adopters” and “non-adopters”. The evidence collected suggests that the adoption of integrated reporting may be driven by the perception of a relative advantage over traditional reporting in terms of providing more relevant information to capital providers, particularly banks. The publication of an integrated report appears to be the result of a process of incremental improvements in the practice of disclosure and assurance of non-financial information, so that the previous experience with sustainability reporting is also an important driver for voluntary integrated reporting. Finally, companies may be differently prepared for transition to integrated reporting and, therefore, have different perceptions of its cost and complexity, which may act as a barrier. Full article

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Open AccessReview

Serine/Threonine Protein Phosphatases 1 and 2A in Lung Endothelial Barrier Regulation

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Biomedicines 2023, 11(6), 1638; https://doi.org/10.3390/biomedicines11061638 (registering DOI) - 05 Jun 2023

Abstract

Vascular barrier dysfunction is characterized by increased permeability and inflammation of endothelial cells (ECs), which are prominent features of acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and sepsis, and a major complication of the SARS-CoV-2 infection and COVID-19. Functional impairment of [...] Read more.

Vascular barrier dysfunction is characterized by increased permeability and inflammation of endothelial cells (ECs), which are prominent features of acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and sepsis, and a major complication of the SARS-CoV-2 infection and COVID-19. Functional impairment of the EC barrier and accompanying inflammation arises due to microbial toxins and from white blood cells of the lung as part of a defensive action against pathogens, ischemia-reperfusion or blood product transfusions, and aspiration syndromes-based injury. A loss of barrier function results in the excessive movement of fluid and macromolecules from the vasculature into the interstitium and alveolae resulting in pulmonary edema and collapse of the architecture and function of the lungs, and eventually culminates in respiratory failure. Therefore, EC barrier integrity, which is heavily dependent on cytoskeletal elements (mainly actin filaments, microtubules (MTs), cell-matrix focal adhesions, and intercellular junctions) to maintain cellular contacts, is a critical requirement for the preservation of lung function. EC cytoskeletal remodeling is regulated, at least in part, by Ser/Thr phosphorylation/dephosphorylation of key cytoskeletal proteins. While a large body of literature describes the role of phosphorylation of cytoskeletal proteins on Ser/Thr residues in the context of EC barrier regulation, the role of Ser/Thr dephosphorylation catalyzed by Ser/Thr protein phosphatases (PPases) in EC barrier regulation is less documented. Ser/Thr PPases have been proposed to act as a counter-regulatory mechanism that preserves the EC barrier and opposes EC contraction. Despite the importance of PPases, our knowledge of the catalytic and regulatory subunits involved, as well as their cellular targets, is limited and under-appreciated. Therefore, the goal of this review is to discuss the role of Ser/Thr PPases in the regulation of lung EC cytoskeleton and permeability with special emphasis on the role of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) as major mammalian Ser/Thr PPases. Importantly, we integrate the role of PPases with the structural dynamics of the cytoskeleton and signaling cascades that regulate endothelial cell permeability and inflammation. Full article

(This article belongs to the Special Issue Vascular Endothelial Functions: Insights from Molecular Perspectives)

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Open AccessArticle

A Label-Free Carbohydrate-Based Electrochemical Sensor to Detect Escherichia coli Pathogenic Bacteria Using D-mannose on a Glassy Carbon Electrode

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Sakineh Hargol Zadeh

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Soheila Kashanian

and

Maryam Nazari

Biosensors 2023, 13(6), 619; https://doi.org/10.3390/bios13060619 (registering DOI) - 05 Jun 2023

Abstract

Controlling water and food contamination by pathogenic organisms requires quick, simple, and low-cost methods. Using the affinity between mannose and type I fimbriae in the cell wall of Escherichia coli (E. coli) bacteria as evaluation elements compared to the conventional plate [...] Read more.

Controlling water and food contamination by pathogenic organisms requires quick, simple, and low-cost methods. Using the affinity between mannose and type I fimbriae in the cell wall of Escherichia coli (E. coli) bacteria as evaluation elements compared to the conventional plate counting technique enables a reliable sensing platform for the detection of bacteria. In this study, a simple new sensor was developed based on electrochemical impedance spectroscopy (EIS) for rapid and sensitive detection of E. coli. The biorecogniton layer of the sensor was formed by covalent attachment of p-carboxyphenylamino mannose (PCAM) to gold nanoparticles (AuNPs) electrodeposited on the surface of a glassy carbon electrode (GCE). The resultant structure of PCAM was characterized and confirmed using a Fourier Transform Infrared Spectrometer (FTIR). The developed biosensor demonstrated a linear response with a logarithm of bacterial concentration (R² = 0.998) in the range of 1.3 × 10 ¹~1.3 × 10⁶ CFU.mL⁻¹ with the limit of detection of 2 CFU.mL⁻¹ within 60 min. The sensor did not generate any significant signals with two non-target strains, demonstrating the high selectivity of the developed biorecognition chemistry. The selectivity of the sensor and its applicability to analysis of the real samples were investigated in tap water and low-fat milk samples. Overall, the developed sensor showed to be promising for the detection of E. coli pathogens in water and low-fat milk due to its high sensitivity, short detection time, low cost, high specificity, and user-friendliness. Full article

(This article belongs to the Topic Advanced Nanomaterials for Sensing Applications)

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Open AccessArticle

Influence of Occlusal Hypofunction on Alveolar Bone Healing in Rats

by

Anna Damanaki

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Svenja Beisel-Memmert

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Marjan Nokhbehsaim

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Ali Abedi

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Birgit Rath-Deschner

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Andressa V. B. Nogueira

and

James Deschner

Int. J. Mol. Sci. 2023, 24(11), 9744; https://doi.org/10.3390/ijms24119744 (registering DOI) - 05 Jun 2023

Abstract

The aim of this in vivo study was to investigate the effect of occlusal hypofunction on alveolar bone healing in the absence or presence of an enamel matrix derivative (EMD). A standardized fenestration defect over the root of the mandibular first molar in [...] Read more.

The aim of this in vivo study was to investigate the effect of occlusal hypofunction on alveolar bone healing in the absence or presence of an enamel matrix derivative (EMD). A standardized fenestration defect over the root of the mandibular first molar in 15 Wistar rats was created. Occlusal hypofunction was induced by extraction of the antagonist. Regenerative therapy was performed by applying EMD to the fenestration defect. The following three groups were established: (a) normal occlusion without EMD treatment, (b) occlusal hypofunction without EMD treatment, and (c) occlusal hypofunction with EMD treatment. After four weeks, all animals were sacrificed, and histological (hematoxylin and eosin, tartrate-resistant acid phosphatase) as well as immunohistochemical analyses (periostin, osteopontin, osteocalcin) were performed. The occlusal hypofunction group showed delayed bone regeneration compared to the group with normal occlusion. The application of EMD could partially, but not completely, compensate for the inhibitory effects of occlusal hypofunction on bone healing, as evidenced by hematoxylin and eosin and immunohistochemistry for the aforementioned molecules. Our results suggest that normal occlusal loading, but not occlusal hypofunction, is beneficial to alveolar bone healing. Adequate occlusal loading appears to be as advantageous for alveolar bone healing as the regenerative potential of EMD. Full article

(This article belongs to the Collection Oral Inflammation—Environment, Molecules, and Cells in Oral Physiology and Pathology)

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Open AccessArticle

Microstructural Evaluation and Tensile Properties of Al-Mg-Sc-Zr Alloys Prepared by LPBF

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Crystals 2023, 13(6), 913; https://doi.org/10.3390/cryst13060913 (registering DOI) - 05 Jun 2023

Abstract

Laser powder bed fusion (LPBF) is a typical additive manufacturing technology that offers significant advantages in the production of complex components. With the rapid heating and cooling characteristics of LPBF, a large amount of solid solution of alloying elements in the matrix can [...] Read more.

Laser powder bed fusion (LPBF) is a typical additive manufacturing technology that offers significant advantages in the production of complex components. With the rapid heating and cooling characteristics of LPBF, a large amount of solid solution of alloying elements in the matrix can be achieved to form supersaturated solid solutions, thus enhancing the properties of LPBF alloys. For the unique microstructure, the heat treatment process needs to be adjusted accordingly. In this work, a Zr/Sc-modified Al-Mg alloy processed by laser powder bed fusion (LPBF) with relatively low cost and good mechanical properties was investigated. The fine microstructure was obtained under rapid solidification conditions. The nanoscale Al₃(Sc,Zr) particles precipitated at the molten pool boundary during solidification. These particles, as effective heterogeneous nucleators, further refined the α-Al grains and improved the mechanical properties of the alloy. As a result, the alloy exhibited a heterogeneous microstructure consisting of columnar grains in the center of the molten pool and equiaxed grains at the boundaries. The rapid solidification resulted in the supersaturation of solute atoms in the α-Al matrix, which significantly enhanced the solid solution strengthening effect. With the LPBF processing parameters of a combination of a laser power of 250 W, a laser scanning speed of 833 mm/s, and stripe scanning mode, the tensile strength of the alloy reached 401.4 ± 5.7 MPa, which was significantly higher than that of the cast alloys with aging treatment (281.1 ± 1.3 MPa). The heat treatment promoted the formation of secondary Al₃(Sc,Zr), Mn/Mg-rich phases. The ultimate tensile strength and elongation at fracture after aging at 325 °C for 2 h were 536.0 ± 1.7 MPa and 14.8 ± 0.8%, respectively. The results provide insight into the preparation of aluminum alloys with relatively low cost and excellent mechanical properties. Full article

(This article belongs to the Special Issue High-Tech Metals - Overview, Microstructure, Properties and Recyclability)

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Open AccessArticle

BIC2, a Cryptochrome Function Inhibitor, Is Involved in the Regulation of ABA Responses in Arabidopsis

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Plants 2023, 12(11), 2220; https://doi.org/10.3390/plants12112220 (registering DOI) - 05 Jun 2023

Abstract

The plant hormone ABA (abscisic acid) is able to regulate plant responses to abiotic stresses via regulating the expression of ABA response genes. BIC1 (Blue-light Inhibitor of Cryptochromes 1) and BIC2 have been identified as the inhibitors of plant cryptochrome functions, and are [...] Read more.

The plant hormone ABA (abscisic acid) is able to regulate plant responses to abiotic stresses via regulating the expression of ABA response genes. BIC1 (Blue-light Inhibitor of Cryptochromes 1) and BIC2 have been identified as the inhibitors of plant cryptochrome functions, and are involved in the regulation of plant development and metabolism in Arabidopsis . In this study, we report the identification of BIC2 as a regulator of ABA responses in Arabidopsis . RT-PCR (Reverse Transcription-Polymerase Chain Reaction) results show that the expression level of BIC1 remained largely unchanged, but that of BIC2 increased significantly in response to ABA treatment. Transfection assays in Arabidopsis protoplasts show that both BIC1 and BIC2 were mainly localized in the nucleus, and were able to activate the expression of the co-transfected reporter gene. Results in seed germination and seedling greening assays show that ABA sensitivity was increased in the transgenic plants overexpressing BIC2, but increased slightly, if any, in the transgenic plants overexpressing BIC1. ABA sensitivity was also increased in the bic2 single mutants in seedling greening assays, but no further increase was observed in the bic1 bic2 double mutants. On the other hand, in root elongation assays, ABA sensitivity was decreased in the transgenic plants overexpressing BIC2, as well as the bic2 single mutants, but no further decrease was observed in the bic1 bic2 double mutants. By using qRT-PCR (quantitative RT-PCR), we further examined how BIC2 may regulate ABA responses in Arabidopsis , and found that inhibition of ABA on the expression of the ABA receptor genes PYL4 (PYR1-Like 4) and PYL5 were decreased, but promotion of ABA on the expression of the protein kinase gene SnRK2.6 (SNF1-Related Protein Kinases 2.6) was enhanced in both the bic1 bic2 double mutants and 35S:BIC2 overexpression transgenic plants. Taken together, our results suggest that BIC2 regulates ABA responses in Arabidopsis possibly by affecting the expression of ABA signaling key regulator genes. Full article

(This article belongs to the Special Issue Trends and Prospects of Genetic and Molecular Research in Plant)

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