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Recent Research in Stem Cells to Organoids
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Recent Research in Stem Cells to Organoids

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 2927

Special Issue Editor

Dr. Meritxell Huch

E-Mail Website
Guest Editor
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
Interests: liver organs

Special Issue Information

Dear Colleagues,

Recent advances in methodologies with which to culture stem cells and organoids have resulted in novel systems that can recapitulate key aspects of animal and human tissue biology. These culture systems are more physiologically relevant than 2D culture models, while providing a reductionist model of in vivo biology in which it is possible to manipulate signaling pathways and perform genome editing. Stem cell and organoid technology is being embraced by researchers globally and is offering novel insights into organogenesis, the pathophysiology of diseases, and regenerative medicine.

This Special Issue aims to promote advances in the stem cell and organoid field and highlight how researchers are using these reduced systems of complex human biology.

This Special Issue calls for papers that use these systems to bridge the transition between molecular and cellular investigations as they build complexity toward tissue, organ, and whole-organism research. We invite authors to submit their original and review articles on these topics.

Dr. Nicole Prior is a scientist (Topical Advisory Panel of the IJMS) and will assist Dr. Meritxell Huch in managing this Special Issue.

Dr. Meritxell Huch
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • organoids
  • organoid technology
  • organogenesis
  • pluripotent stem cells
  • iPSCs
  • organoid
  • stem cell niche
  • disease modeling
  • drug screening
  • regenerative medicine

Published Papers (3 papers)

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Research

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11 pages, 4733 KiB  
Article
Fetal Kidney Grafts and Organoids from Microminiature Pigs: Establishing a Protocol for Production and Long-Term Cryopreservation
by Yuka Inage, Koki Fujimori, Masaki Takasu, Kenji Matsui, Yoshitaka Kinoshita, Keita Morimoto, Nagisa Koda, Shutaro Yamamoto, Kentaro Shimada, Takashi Yokoo and Eiji Kobayashi
Int. J. Mol. Sci. 2024, 25(9), 4793; https://doi.org/10.3390/ijms25094793 (registering DOI) - 27 Apr 2024
Viewed by 137
Abstract
Fetal organs and organoids are important tools for studying organ development. Recently, porcine organs have garnered attention as potential organs for xenotransplantation because of their high degree of similarity to human organs. However, to meet the prompt demand for porcine fetal organs by [...] Read more.
Fetal organs and organoids are important tools for studying organ development. Recently, porcine organs have garnered attention as potential organs for xenotransplantation because of their high degree of similarity to human organs. However, to meet the prompt demand for porcine fetal organs by patients and researchers, effective methods for producing, retrieving, and cryopreserving pig fetuses are indispensable. Therefore, in this study, to collect fetuses for kidney extraction, we employed cesarean sections to preserve the survival and fertility of the mother pig and a method for storing fetal kidneys by long-term cryopreservation. Subsequently, we evaluated the utility of these two methods. We confirmed that the kidneys of pig fetuses retrieved by cesarean section that were cryopreserved for an extended period could resume renal growth when grafted into mice and were capable of forming renal organoids. These results demonstrate the usefulness of long-term cryopreserved fetal pig organs and strongly suggest the effectiveness of our comprehensive system of pig fetus retrieval and fetal organ preservation, thereby highlighting its potential as an accelerator of xenotransplantation research and clinical innovation. Full article
(This article belongs to the Special Issue Recent Research in Stem Cells to Organoids)
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14 pages, 1536 KiB  
Article
Characterization of 3D Organotypic Culture of Mouse Adipose-Derived Stem Cells
by Tae Gen Son, Yoojin Seo, Won-Tae Kim, Meesun Kim, Seon Jeong Choi, Si Ho Choi, Byung-Jun Sung, Jae-Seok Min, Eon Chul Han and Hyung-Sik Kim
Int. J. Mol. Sci. 2024, 25(7), 3931; https://doi.org/10.3390/ijms25073931 - 01 Apr 2024
Viewed by 500
Abstract
Although stem cells are a promising avenue for harnessing the potential of adipose tissue, conventional two-dimensional (2D) culture methods have limitations. This study explored the use of three-dimensional (3D) cultures to preserve the regenerative potential of adipose-derived stem cells (ADSCs) and investigated their [...] Read more.
Although stem cells are a promising avenue for harnessing the potential of adipose tissue, conventional two-dimensional (2D) culture methods have limitations. This study explored the use of three-dimensional (3D) cultures to preserve the regenerative potential of adipose-derived stem cells (ADSCs) and investigated their cellular properties. Flow cytometric analysis revealed significant variations in surface marker expressions between the two culture conditions. While 2D cultures showed robust surface marker expressions, 3D cultures exhibited reduced levels of CD44, CD90.2, and CD105. Adipogenic differentiation in 3D organotypic ADSCs faced challenges, with decreased organoid size and limited activation of adipogenesis-related genes. Key adipocyte markers, such as lipoprotein lipase (LPL) and adipoQ, were undetectable in 3D-cultured ADSCs, unlike positive controls in 2D-cultured mesenchymal stem cells (MSCs). Surprisingly, 3D-cultured ADSCs underwent mesenchymal–epithelial transition (MET), evidenced by increased E-cadherin and EpCAM expression and decreased mesenchymal markers. This study highlights successful ADSC organoid formation, notable MSC phenotype changes in 3D culture, adipogenic differentiation challenges, and a distinctive shift toward an epithelial-like state. These findings offer insights into the potential applications of 3D-cultured ADSCs in regenerative medicine, emphasizing the need for further exploration of underlying molecular mechanisms. Full article
(This article belongs to the Special Issue Recent Research in Stem Cells to Organoids)
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Review

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28 pages, 2947 KiB  
Review
Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm
by Vojtech Gabriel, Christopher Zdyrski, Dipak K. Sahoo, Abigail Ralston, Hannah Wickham, Agnes Bourgois-Mochel, Basant Ahmed, Maria M. Merodio, Karel Paukner, Pablo Piñeyro, Jamie Kopper, Eric W. Rowe, Jodi D. Smith, David Meyerholz, Amir Kol, Austin Viall, Mohamed Elbadawy, Jonathan P. Mochel and Karin Allenspach
Int. J. Mol. Sci. 2024, 25(2), 701; https://doi.org/10.3390/ijms25020701 - 05 Jan 2024
Cited by 1 | Viewed by 1652
Abstract
Preclinical biomedical research is limited by the predictiveness of in vivo and in vitro models. While in vivo models offer the most complex system for experimentation, they are also limited by ethical, financial, and experimental constraints. In vitro models are simplified models that [...] Read more.
Preclinical biomedical research is limited by the predictiveness of in vivo and in vitro models. While in vivo models offer the most complex system for experimentation, they are also limited by ethical, financial, and experimental constraints. In vitro models are simplified models that do not offer the same complexity as living animals but do offer financial affordability and more experimental freedom; therefore, they are commonly used. Traditional 2D cell lines cannot fully simulate the complexity of the epithelium of healthy organs and limit scientific progress. The One Health Initiative was established to consolidate human, animal, and environmental health while also tackling complex and multifactorial medical problems. Reverse translational research allows for the sharing of knowledge between clinical research in veterinary and human medicine. Recently, organoid technology has been developed to mimic the original organ’s epithelial microstructure and function more reliably. While human and murine organoids are available, numerous other organoids have been derived from traditional veterinary animals and exotic species in the last decade. With these additional organoid models, species previously excluded from in vitro research are becoming accessible, therefore unlocking potential translational and reverse translational applications of animals with unique adaptations that overcome common problems in veterinary and human medicine. Full article
(This article belongs to the Special Issue Recent Research in Stem Cells to Organoids)
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