Application of LC-MS/MS in Food Analysis and Quality Control

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: closed (28 February 2023) | Viewed by 11249

Special Issue Editors


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Guest Editor
I.U. CINQUIMA, Analytical Chemistry Group, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain
Interests: chromatography; separation science; food analysis; food safety; pharmaceutical analysis; environmental analysis; method development; method validation; food quality
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
I.U. CINQUIMA, Analytical Chemistry Group, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain
Interests: chromatography; separation science; food analysis; food safety; pharmaceutical analysis; quality control; enviromental analysis; method development
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Liquid chromatography (LC) is probably one the most used analytical technique in the separation of individual compounds in foods. It is usually applied in the characterization of bioactive compounds and aroma-related compounds to foods, or in the determination of food contaminants such as pesticides, veterinary drugs or antibiotics. In the last years, its coupling with tandem mass spectrometry (MS/MS) has increasingly been used for Food Analysis and Quality Control. This is probably due to the high performance in terms of sensitivity, selectivity, and flexibility, which is quite important when addressing various issues concerning the food safety. Many risk factors, such as agricultural chemical residues, residual veterinary drugs, mycotoxins, food additives, and carcinogens produced during food processing may be present in foodstuffs. In addition, food contains a wide variety of nutritional components, which determination is of great importance in order to increase and demonstrate the nutritional value of these products. However, it should be considered that in most cases, food matrices are complex ones that present several interfering compounds that could affect to the LC-MS/MS determination. Then, sample preparation to remove such interference and an appropriate choice of an analytical technique is required. Even with LC-MS/MS, the analytical reliability may be reduced by matrix effects, due to interference from food components. Thus, this Special Issue on “Application of LC-MS/MS in Food Analysis and Quality Control” is a unique opportunity to gather state-of-the-art of LC-MS/MS-based methods to control the safety and bioactivity of foods, as well as its quality control. Therefore, papers dealing with both the LC-MS/MS method development and with their application on food analysis and quality control, will be welcome.

Prof. Dr. José Bernal del Nozal
Prof. Dr. Ana M. Ares
Guest Editors

Manuscript Submission Information

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Keywords

  • Bioactive compounds
  • Chemical residues
  • Food authentication
  • Mycotoxins
  • Organic pollutants
  • Pesticide residues
  • Phytochemicals
  • Organic pollutants
  • Safety of food commodities
  • Veterinary drugs
  • Antibiotics
  • Food analysis
  • Food quality
  • Food safety
  • LC-MS/MS
  • UHPLC-MS/MS

Published Papers (5 papers)

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Research

13 pages, 6428 KiB  
Article
Metabolomics Characterize the Differential Metabolic Markers between Bama Xiang Pig and Debao Pig to Identify Pork
by Changyi Chen, Junwen Zheng, Chenyong Xiong, Hongjin Zhou, Chuntao Wei, Xin Hu, Xinxiu Qian, Mengyi He, Yandi Shi, Yuwen Liu and Zongqiang Li
Foods 2023, 12(1), 5; https://doi.org/10.3390/foods12010005 - 20 Dec 2022
Cited by 1 | Viewed by 1412
Abstract
The Bama Xiang pig (BM) is a unique pig species in Guangxi Province, China. Compared to other breeds of domestic pig, such as the Debao pig (DB), it is smaller in size, better in meat quality, resistant to rough feeding and strong in [...] Read more.
The Bama Xiang pig (BM) is a unique pig species in Guangxi Province, China. Compared to other breeds of domestic pig, such as the Debao pig (DB), it is smaller in size, better in meat quality, resistant to rough feeding and strong in stress resistance. These unique advantages of Bama Xiang pigs make them of great edible value and scientific research value. However, the differences in muscle metabolites between Bama Xiang pigs (BM) and Debao pigs (DB) are largely unexplored. Here, we identified 214 differential metabolites between these two pig breeds by LC-MS. Forty-one such metabolites are enriched into metabolic pathways, and these metabolites correspond to 11 metabolic pathways with significant differences. In Bama pigs, the abundance of various metabolites such as creatine, citric acid, L-valine and hypoxanthine is significantly higher than in Debao pigs, while the abundance of other metabolites, such as carnosine, is significantly lower. Among these, we propose six differential metabolites: L-proline, citric acid, ribose 1-phosphate, L-valine, creatine, and L-arginine, as well as four potential differential metabolites (without the KEGG pathway), alanyl-histidine, inosine 2′-phosphate, oleoylcarnitine, and histidinyl hydroxyproline, as features for evaluating the meat quality of Bama pigs and for differentiating pork from Bama pigs and Debao pigs. This study provides a proof-of-concept example of distinguishing pork from different pig breeds at the metabolite level and sheds light on elucidating the biological processes underlying meat quality differences. Our pork metabolites data are also of great value to the genomics breeding community in meat quality improvement. Full article
(This article belongs to the Special Issue Application of LC-MS/MS in Food Analysis and Quality Control)
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16 pages, 2117 KiB  
Article
Evaluating the Impacts of Climate Factors and Flavonoids Content on Chinese Prickly Ash Peel Color Based on HPLC-MS and Structural Equation Model
by Tao Zheng, Ding-Ling Zhang, Bing-Yin Sun and Shu-Ming Liu
Foods 2022, 11(16), 2539; https://doi.org/10.3390/foods11162539 - 22 Aug 2022
Cited by 6 | Viewed by 1479
Abstract
Climate affects Chinese prickly ash peel color directly through temperature and illumination and indirectly influences it through its effect on flavonoid compounds. In this study, a comprehensive evaluation strategy based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technology and a structural equation model [...] Read more.
Climate affects Chinese prickly ash peel color directly through temperature and illumination and indirectly influences it through its effect on flavonoid compounds. In this study, a comprehensive evaluation strategy based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technology and a structural equation model was applied to evaluate the effects of climate factors and flavonoids on Chinese prickly ash peel color. There were obvious geographical variations of peel color and flavonoid compounds with an obvious east–west distribution trend which were divided into high-altitude type and low-altitude type. Through path analysis, the wind speed, temperature and annual sunshine duration were found to be the key environmental factors affecting the flavonoids content and peel color, and their direct effects were higher than their indirect effect. Based on HPLC-MS technology and a structural equation model, correlation models of climatic factors and flavonoids with peel color were established, and the factors that had greater weight on pericarp color were obtained. Our results provide experimental evidence that climate factors affect the peel color by affecting flavonoid biosynthesis and accumulation, reveal the geographical variation of peel color and flavonoid component contents in Chinese prickly ash peel, establish a quantization color method for rapid evaluation of peel quality, expand on the influence of climatic factors on flavonoids content and peel coloration and promote agricultural practice in areas with similar climatic conditions. Full article
(This article belongs to the Special Issue Application of LC-MS/MS in Food Analysis and Quality Control)
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13 pages, 3454 KiB  
Article
Simultaneous Determination of Nine Quinolones in Pure Milk Using PFSPE-HPLC-MS/MS with PS-PAN Nanofibers as a Sorbent
by Lanlan Wei, Yanan Chen, Dongliang Shao and Jingjun Li
Foods 2022, 11(13), 1843; https://doi.org/10.3390/foods11131843 - 22 Jun 2022
Cited by 7 | Viewed by 1687
Abstract
In this study, a packed-fiber solid-phase extraction (PFSPE)-based method was developed to simultaneously detect nine quinolones, including enrofloxacin (ENR), ciprofloxacin (CIP), ofloxacin (OFL), pefloxacin (PEF), lomefloxacin (LOM), norfloxacin (NOR), sarafloxacin (SAR), danofloxacin (DAN), and difloxacin (DIF), in pure milk, using high-performance liquid chromatography [...] Read more.
In this study, a packed-fiber solid-phase extraction (PFSPE)-based method was developed to simultaneously detect nine quinolones, including enrofloxacin (ENR), ciprofloxacin (CIP), ofloxacin (OFL), pefloxacin (PEF), lomefloxacin (LOM), norfloxacin (NOR), sarafloxacin (SAR), danofloxacin (DAN), and difloxacin (DIF), in pure milk, using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Polystyrene (PS) and polyacrylonitrile (PAN) were combined to form PS-PAN composite nanofibers through electrospinning. The nanofibers were used to prepare the home-made extraction columns, and the process was optimized and validated using blank pure milk. The analytical method showed high accuracy, and the recoveries were 88.68–97.63%. Intra-day and inter-day relative standard deviations were in the ranges of 1.11–6.77% and 2.26–7.17%, respectively. In addition, the developed method showed good linearity (R2 ≥ 0.995) and low method quantification limits for the nine quinolones (between 1.0–100 ng/mL) for all samples studied. The nine quinolones in the complex matrix were directly extracted using 4.0 mg of PS-PAN composite nanofibers as a sorbent and completely eluted in 100 μL elution solvent. Therefore, the developed PFSPE-HPLC-MS/MS is a sensitive and cost-effective technique that can effectively detect and control nine quinolones in dairy products. Full article
(This article belongs to the Special Issue Application of LC-MS/MS in Food Analysis and Quality Control)
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14 pages, 1322 KiB  
Article
Glucosinolates as Markers of the Origin and Harvesting Period for Discrimination of Bee Pollen by UPLC-MS/MS
by Ana M. Ares, Jesús A. Tapia, Amelia V. González-Porto, Mariano Higes, Raquel Martín-Hernández and José Bernal
Foods 2022, 11(10), 1446; https://doi.org/10.3390/foods11101446 - 17 May 2022
Cited by 6 | Viewed by 1706
Abstract
Bee pollen is currently one of the most commonly consumed food supplements, as it is considered to be a good source of bioactive substances and energy. It contains various health-promoting compounds, such as proteins, amino acids, lipids, as well as glucosinolates. In the [...] Read more.
Bee pollen is currently one of the most commonly consumed food supplements, as it is considered to be a good source of bioactive substances and energy. It contains various health-promoting compounds, such as proteins, amino acids, lipids, as well as glucosinolates. In the present study, the glucosinolate content was determined, by means of ultra-performance liquid chromatography coupled to a quadrupole time-of-flight mass detector, in 72 bee pollen samples from four different apiaries in Guadalajara (Spain), harvested in three different periods. In addition, 11 commercial multifloral samples from different Spanish regions were also analyzed. The aim was to verify the suitability of these compounds as biomarkers of their geographical origin, and to test their potential for distinguishing the harvesting period. By means of a canonical discriminant analysis, it was possible to differentiate the apiary of origin of most of the samples, and these could also be clearly differentiated from the commercial ones, simply as a result of the glucosinolate content. In addition, it was also demonstrated for the first time that bee pollen samples were capable of being differentiated according to the time of harvesting and their glucosinolate content. Full article
(This article belongs to the Special Issue Application of LC-MS/MS in Food Analysis and Quality Control)
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13 pages, 1550 KiB  
Article
Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from Momordica charantia L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells
by Yu-Tsung Lee, Li-Heng Pao, Chi-Yuan Chen, Sui-Qing Huang, Alaganandam Kumaran, Jong-Ho Chyuan and Chun-Hui Chiu
Foods 2022, 11(5), 729; https://doi.org/10.3390/foods11050729 - 01 Mar 2022
Cited by 4 | Viewed by 2311
Abstract
Cucurbitane-type triterpenoids are a major class of bioactive compounds present in bitter melon. In the present study, six different cultivars of bitter melon were extracted by using microwave- or ultrasound-assisted techniques to identify the prominent method that can extract the majority of cucurbitane-type [...] Read more.
Cucurbitane-type triterpenoids are a major class of bioactive compounds present in bitter melon. In the present study, six different cultivars of bitter melon were extracted by using microwave- or ultrasound-assisted techniques to identify the prominent method that can extract the majority of cucurbitane-type triterpenoids. A UHPLC–MS/MS (ultra-high-performance liquid chromatography tandem mass spectrometry) system was used for the identification and quantification of ten cucurbitane-type triterpenoids. The results suggest that the use of microwave-assisted extraction on cultivars 4 and 5 produced higher amounts of the selected cucurbitane-type triterpenoids. The interpretation of principal component analysis also identified that cultivar 4 is significantly different from the others in which the compounds 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicine I were found in higher quantities. Upon further evaluation, it was also identified that these two triterpenoids can act as antiproliferative agents due to their effects on SAS human oral cancer cell lines. Full article
(This article belongs to the Special Issue Application of LC-MS/MS in Food Analysis and Quality Control)
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