Editorial

5 pages, 198 KiB

Open AccessEditorial

Dairy Fermentation

by Thomas Bintsis

Fermentation 2023, 9(3), 252; https://doi.org/10.3390/fermentation9030252 - 04 Mar 2023

Viewed by 1409

Fermented dairy products have been traditionally produced and fermentation has evolved as an effective technique to preserve milk from various milking animals [...] Full article

(This article belongs to the Special Issue Dairy Fermentation)

Research

Jump to: Editorial, Review

11 pages, 2821 KiB

Open AccessArticle

Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of Lactobacillus acidophilus

by Norma Angélica Bolivar-Jacobo, Raúl Alberto Reyes-Villagrana, Ana Luisa Rentería-Monterrubio, Rogelio Sánchez-Vega, Eduardo Santellano-Estrada, Juan Manuel Tirado-Gallegos and América Chávez-Martínez

Fermentation 2023, 9(1), 63; https://doi.org/10.3390/fermentation9010063 - 12 Jan 2023

Cited by 6 | Viewed by 2651

Abstract

The growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the growth medium [...] Read more.

The growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the growth medium (MRS broth, whole milk, and skim milk), culture age (1 day and 7 days old) and ultrasound parameters (time and amplitude) on the kinetic parameters of L. acidophilus. The oldest culture (7 days) had a greater lag phase and time to reach the end of the sigmoidal curve (T_max) (p < 0.05) as well as a lower rate (maximum growth potential μ_max) compared to the youngest culture (1 day). Regarding the growth medium, skim milk presented the greatest L. acidophilus counts (p < 0.05). Meanwhile, sonication times (60 and 90 s) change µ_max and T_max. When 30% amplitude was applied, a greater μ_max and a smaller T_max were observed (p < 0.05). It can be concluded that the growth medium, culture age, and ultrasound parameters (time and amplitude) influence the kinetic parameters of L. acidophilus. Results from this study could be used in the design and optimization of processes to improve the growth of the probiotic L. acidophilus at industrial scale. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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12 pages, 672 KiB

Open AccessArticle

Fermentation of Dietary Fibre-Added Milk with Yoghurt Bacteria and L. rhamnosus and Use in Ice Cream Production

by Elif Sezer, Ahmet Ayar and Suzan Öztürk Yılmaz

Fermentation 2023, 9(1), 3; https://doi.org/10.3390/fermentation9010003 - 21 Dec 2022

Cited by 6 | Viewed by 4023

Abstract

This study investigated whether the use of fermented milk with added dietary fibre in ice cream production positively affected quality characteristics, especially viability, during a shelf life of 90 days at −25 °C. For this purpose, fermented milk was prepared with cultures (yoghurt [...] Read more.

This study investigated whether the use of fermented milk with added dietary fibre in ice cream production positively affected quality characteristics, especially viability, during a shelf life of 90 days at −25 °C. For this purpose, fermented milk was prepared with cultures (yoghurt and Lacticaseibacillus rhamnosus) and dietary fibre (wheat fibre and inulin). In addition to the viable cell count, some related quality characteristics, such as the sensory, physical, chemical, and thermal properties, and energy content were also examined. Streptococcus salivarius subsp. thermophilus in all yoghurt ice creams and L. rhamnosus in ice cream with wheat fibre had the highest viability for 90 days, up to 95.95%. The best scores regarding “general acceptability” belonged to the ice cream with L. rhamnosus and inulin, with a score of 7.81 out of 9. The dietary fibre decreased overrun from around 23% to 14–18%, which was positive for the viability of the cultures. The cultures and dietary fibre decreased the melting temperature down to −1.15 °C. The caloric value of the ice creams (166–168 kcal/100 g) was lower than that of standard ice cream. Probiotic ice cream production with dietary fibre and a single L. rhamnosus culture may be preferred in terms of sensory properties, cell viability, and economic aspects. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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Graphical abstract

15 pages, 1640 KiB

Open AccessArticle

Evaluation of the Thermal Processes on Changing the Phenotypic Characteristics of Escherichia coli Strains from Ice Cream Compared to Non-Pasteurized Milk

by Maryam Ranjbar, Reza Nedaeinia, Mohammad Goli and Sharifeh Shahi

Fermentation 2022, 8(12), 730; https://doi.org/10.3390/fermentation8120730 - 12 Dec 2022

Cited by 1 | Viewed by 1462

Abstract

Escherichia coli (E. coli) is shocked by various temperature processes in milk, which forces the organism to make proteins as a result of changes in the synthesis of enzymes that might give the strain special characteristics. The purpose of this study [...] Read more.

Escherichia coli (E. coli) is shocked by various temperature processes in milk, which forces the organism to make proteins as a result of changes in the synthesis of enzymes that might give the strain special characteristics. The purpose of this study was to investigate the effects of the heat shock factor on changing the results of biochemical and molecular tests among E. coli strains obtained from ice cream and non-pasteurized milk when compared to a reference strain from the American-type culture collection (ATCC) in order to determine the phenotypic variation caused by the temperature conditions of the manufacturing process. Furthermore, isolates with characteristics similar to E. coli were discovered, but they were not E. coli and caused some ambiguity. To test the E. coli contamination of traditional and industrial ice cream, 82 samples were chosen at random. SDS-PAGE and 16S rDNA sequencing were carried out, as well as phenotypic testing. Isolated strains did not exactly match the reference strain. The results of biochemical testing and protein analysis revealed that the isolates were diverse. Samples E. coli phenons were classified. In the electrophoresis, the ice cream strain had two protein bands in the 20.75 and 23.59 kDa ranges that were distinct from the reference strain. These isolates appear to experience alterations in enzyme characteristics and structural proteins as a result of being exposed to various temperature conditions, such as pasteurization and frigidity. When compared to the reference strain, the calculated similarity percentage of the elicited isolate varied from 60 to 70%. The electrophoretic patterns of E. coli isolated elicited from milk samples differed from E. coli isolated obtained from the ice cream. The distinctions were in the intensity or position of the bands. The results also revealed that when isolates are subjected to thermal stresses, they exhibit a pattern similar to that of ice cream isolates. These considerations are made because a change in protein composition might result in a change in biochemical features, resulting in uncertainty in its identification. Sequences revealed that the sequences were related to E. coli 16S rDNA, despite differences in phenotypic and electrophoretic features between the isolated bacteria and the reference strain E. coli ATCC 25922. Our findings revealed that 16S rDNA could potentially be used to instantly implement an appropriate preventive measure for the purpose of identifying this type of bacteria and avoid some ambiguity. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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22 pages, 5856 KiB

Open AccessArticle

Isolation of Yeasts from Some Homemade Fermented Cow-Milk Products of Sikkim and Their Probiotic Characteristics

by Sonam Lama and Jyoti Prakash Tamang

Fermentation 2022, 8(12), 664; https://doi.org/10.3390/fermentation8120664 - 23 Nov 2022

Cited by 6 | Viewed by 2849

Abstract

Dahi and chhurpi are the homemade, mildly acidic and mouthfeel fermented dairy products of Sikkim in India. Since yeasts co-exist among traditional fermented dairy foods, we believe that some species of yeasts may have some probiotic properties. Hence, the present study is aimed [...] Read more.

Dahi and chhurpi are the homemade, mildly acidic and mouthfeel fermented dairy products of Sikkim in India. Since yeasts co-exist among traditional fermented dairy foods, we believe that some species of yeasts may have some probiotic properties. Hence, the present study is aimed at screening some probiotic yeasts from dahi and chhurpi. A total of 3438 yeasts were isolated from 40 samples of dahi (1779 isolates) and 40 chhurpi (1659 isolates) and were preliminarily screened for probiotic properties on the basis of survival in low pH, resistance to bile salts and the percentage of hydrophobicity, out of which only 20 yeasts were selected for in vitro and genetic screening of probiotic properties. Saccharomyces cerevisiae DJT-2 and Debaryomyces prosopidis CPA-55 showed the highest hydrophobicity of 97.54% and 98.33%, respectively. S. cerevisiae DRC-42 and S. cerevisiae CGI-29 showed 93.88% and 91.69% auto-aggregation, respectively. All yeasts showed co-aggregation properties against pathogenic bacteria. Kluyveromyces marxianus DPA-41 and Pichia kudriavzevii CNT-3 showed excellent deconjugation activities. Probiotic genes for acid tolerance, bile tolerance, adhesion and antimicrobial activity were detected in S. cerevisiae DAO-17, K. marxianus DPA-41, S. cerevisiae CKL-10 and P. kudriavzevii CNT-3. Based on the results of in vitro and genetic screening of probiotic yeasts strains, S. cerevisiae DAO-17 (dahi), S. cerevisiae CKL-10 (chhurpi), P. kudriavzevii CNT-3 (chhurpi) and K. marxianus DPA-41(dahi) were selected as the potential probiotic yeasts. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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17 pages, 3877 KiB

Open AccessArticle

Effect of Intermittent Fasting, Probiotic-Fermented Camel Milk, and Probiotic-Fermented Camel Milk Incorporating Sukkari Date on Diet-Induced Obesity in Rats

by Thamer Aljutaily, Medhat Rehan, Mahmoud M. A. Moustafa and Hassan Barakat

Fermentation 2022, 8(11), 619; https://doi.org/10.3390/fermentation8110619 - 09 Nov 2022

Cited by 5 | Viewed by 1835

Abstract

Obesity causes metabolic syndrome disorders that are detrimental to health. The current study examined the effects of intermittent fasting (IF), fermented camel milk (FCM), and fermented camel milk incorporating 10% Sukkari date (FCM-D) on weight loss, blood profile, and antioxidant status in obese [...] Read more.

Obesity causes metabolic syndrome disorders that are detrimental to health. The current study examined the effects of intermittent fasting (IF), fermented camel milk (FCM), and fermented camel milk incorporating 10% Sukkari date (FCM-D) on weight loss, blood profile, and antioxidant status in obese rats for 6 weeks. Subsequently, leptin and adiponectin levels and histopathological examination of adipose tissue were carried out. Results showed that IF with FCM or FCM-D decreased body weight by 0.92 and −5.45%, respectively. IF alone lowered non-fasting blood glucose (NFBG) and fasting blood glucose FBG after 6 weeks, whereas adding FCM or FCM-D reduced NFBG after 4 weeks. Intermittently fasting obese rats given FCM or FCM-D had the lowest blood glucose levels (BGL). The hypolipidemic effects of IF, FCM, and FCM-D on obese rats reduced triglycerides (TG), cholesterols (CHO), and their derivatives. FCM-D with IF presented a superior effect on lipid profile. A reduction rate of 40, 37, 66, and 40% for TG, CHO, low-density lipoprotein (LDL-c), and very low-density lipoprotein (VLDL-c), respectively, and an increase in HDL-C by 34% were noticed. Reductions of 40, 37, 66, and 40% for TG, CHO, LDL-c, and VLDL-c, respectively, and a 34% rise in high-density lipoprotein (HDL-C) were noted. Combining IF with FCM or FCM-D lowered the atherogenic index (AI) by 42% and 59%, respectively. Remarkably, treating rats with FCM+IF or FCM-D+IF effectively attenuated leptin and adiponectin levels. Malondialdehyde (MDA) was significantly decreased in a type-dependent manner. Implementing FCM-D or FCM with IF significantly attenuated reduced glutathione (GSH), superoxide dismutase (SOD), MDA, and catalase (CAT) levels. The most efficient treatment was giving FCM-D with IF. Histopathologically, adipocyte lipolysis increases free fatty acids (FFAs) and promotes inflammation. Only IF+FCM-D indicated no histopathological alteration except for a few focal areas of a few inflammatory cell infiltrations in the parenchyma. In conclusion, combining IF and Probiotic-FCM or Probiotic-FCM-D effectively accelerated weight loss, attenuated metabolic markers, and reversed histopathological alterations. Thus, IF combined with Probiotic-FCM or Probiotic-FCM-D is highly recommended for weight loss, strengthening antioxidative status, and preventing health disorders. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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16 pages, 1427 KiB

Open AccessArticle

Grape Pomace in Ewes Diet Affects Metagenomic Profile, Volatile Compounds and Biogenic Amines Contents of Ripened Cheese

by Francesca Bennato, Marco Di Domenico, Andrea Ianni, Luigina Di Gialleonardo, Cesare Cammà and Giuseppe Martino

Fermentation 2022, 8(11), 598; https://doi.org/10.3390/fermentation8110598 - 02 Nov 2022

Cited by 2 | Viewed by 1425

Abstract

The main objective of this research was to evaluate the development of volatile organic compounds (VOCs) and the accumulation of biogenic amines (BAs) in relation to the dynamic of microbial population composition in fresh and ripened cheese produced from raw milk of ewes [...] Read more.

The main objective of this research was to evaluate the development of volatile organic compounds (VOCs) and the accumulation of biogenic amines (BAs) in relation to the dynamic of microbial population composition in fresh and ripened cheese produced from raw milk of ewes fed a diet containing grape pomace (GP+) and fed a standard diet (Ctrl). Genomic DNA was extracted from the cheeses at 2 (T2), 60 (T60), 90 (T90) and 120 (T120) days of ripening and prepared for 16S rRNA-gene sequencing to characterize the cheese microbiota; furthermore, VOCs were determined via solid-phase microextraction combined with gas chromatography-mass spectrometry and biogenic amines by HPLC analyses. Diet did not affect the relative abundance of the main phyla identified, Proteobacteria characterized T2 samples, but the scenario changed during the ripening. At genus level, Pseudomonas, Chryseobacterium and Acinetobacter were the dominant taxa, however, a lower percentage of Pseudomonas was detected in GP+ cheeses. Enterococcus became dominant in ripened cheeses followed in Ctrl cheeses by Lactobacillus and in GP+ cheeses by Lactococcus. The diet affected the development of carboxylic acids and ketones but not of aldehydes. Low levels of esters were identified in all the samples. In total, four biogenic amines were determined in cheeses samples and their levels differed between the two groups and during ripening time. In 60, T90 and T120 GP+ cheeses, a lower amount of 2-phenylethylamine was found compared to Ctrl. Putrescine was detected only in GP+ samples and reached the highest level at 120 days. Conversely, the amount of cadaverine in GP+ samples was invariable during the ripening. The concentration of tyramine in GP+ samples was compared to Ctrl during the ripening. Overall, significant positive correlations between some families of bacteria and the formation of VOCs and BAs were found. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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13 pages, 817 KiB

Open AccessArticle

Nutritional Composition and Health-Promoting Properties of Amasi: A South African Fermented Milk Product

by Mpho Sebabiki Maleke and Oluwafemi Ayodeji Adebo

Fermentation 2022, 8(10), 493; https://doi.org/10.3390/fermentation8100493 - 28 Sep 2022

Cited by 1 | Viewed by 1965

Abstract

This study investigated the effect of fermentation conditions (time and temperature) on the nutritional composition and health-promoting properties of raw milk, optimized naturally fermented amasi, and amasi fermented with starter culture. The composition of the products, including chemical analysis, amino acids, mineral [...] Read more.

This study investigated the effect of fermentation conditions (time and temperature) on the nutritional composition and health-promoting properties of raw milk, optimized naturally fermented amasi, and amasi fermented with starter culture. The composition of the products, including chemical analysis, amino acids, mineral composition, and fatty acids, as well as health-promoting properties (total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity assays (2,2-Azinobis (3-Ethyl-Benzothiazone-6-Sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) were investigated. The results showed that the fermentation conditions (time and temperature) significantly affected the ash contents of both fermented samples. Fermentation was also observed to have increased the contents of most of the essential and non-essential amino acids. A high concentration of glutamic acid was observed in optimized naturally fermented amasi at 32 °C (OP32, 6.30 g/100 g), followed by starter-culture-fermented amasi at 25 °C (SC25, 5.58 g/100 g) and then starter-culture-fermented amasi at 32 °C (SC32, 5.35 g/100 g), with optimized naturally fermented amasi at 32 °C (OP25, 4.46 g/100 g) having the lowest concentration. A similar trend in the composition of minerals was also observed, with potassium being the most abundant (110.1–134.5 µg/g). The TPC, TFC, and the antioxidant activities were significantly improved by fermentation, while a significant reduction in free fatty acids was recorded. In conclusion, the fermentation conditions had a significant effect on the nutritional and health-promoting properties of amasi. The results provided in this study could provide nutritional information regarding the composition of amasi, which can be beneficial to produce a high-quality product. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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22 pages, 392 KiB

Open AccessArticle

Microbiological Characterization of Greek Galotyri Cheese PDO Products Relative to Whether They Are Marketed Fresh or Ripened

by John Samelis and Athanasia Kakouri

Fermentation 2022, 8(10), 492; https://doi.org/10.3390/fermentation8100492 - 28 Sep 2022

Cited by 8 | Viewed by 1543

Abstract

Galotyri is the most popular traditional Greek PDO soft acid-curd cheese. This study compared the microbial numbers and types and characterized the lactic acid bacteria (LAB) biota of two artisan-type Galotyri PDO cheese varieties, one marketed fresh (Brand-K) and the other ripened (Brand-Z). [...] Read more.

Galotyri is the most popular traditional Greek PDO soft acid-curd cheese. This study compared the microbial numbers and types and characterized the lactic acid bacteria (LAB) biota of two artisan-type Galotyri PDO cheese varieties, one marketed fresh (Brand-K) and the other ripened (Brand-Z). Two retail batches of each cheese variety were analyzed, and a total of 102 LAB isolates were biochemically identified. LAB (7.2–9.3 log CFU/g) prevailed in all cheeses, followed by yeasts (5.8–6.8 log CFU/g). Typical starter strains of Streptococcus thermophilus and Lactobacillus delbrueckii were the most abundant species in all batches. However, the fresh Brand-K cheeses had 1–3 log units higher thermophilic starter LAB counts than the ripened Brand-Z cheeses, which contained a more diverse viable LAB biota comprising Lacticaseibacillus paracasei, Leuconostoc mesenteroides, Lentilactobacillus (L. diolivorans, L. kefiri, L. hilgardii), Pediococcus inopinatus/parvulus, few spontaneous nonstarter thermophilic streptococci and lactobacilli, and Enterococcus faecium and E. faecalis at higher subdominant levels.Conversely, the fresh Brand-K cheeses were enriched in members of the Lactiplantibacillus plantarum group; other LAB species were sporadically isolated, including Lactococcus lactis. All retail cheeses were safe (pH 3.9–4.0). No Salmonella spp. or Listeria monocytogenes were detected in 25-g samples by culture enrichment; however, Listeria innocua and coagulase-positive staphylococci (850 CFU/g) survived in one ripened batch. Gram-negative bacteria were <100 CFU/g in all cheeses. In conclusion, ripening reduced the starter LAB viability but increased the nonstarter LAB species diversity in the present Galotyri PDO market cheeses. Full article

(This article belongs to the Special Issue Dairy Fermentation)

13 pages, 288 KiB

Open AccessArticle

Insights into the Microbiological and Physicochemical Properties of Bio-Frozen Yoghurt Made with Probiotic Strains in Combination with Jerusalem Artichoke Tubers Powder

by Magdy Ramadan Shahein, Wael F. Elkot, Nisreen Khalid Aref Albezrah, Lina Jamil M. Abdel-Hafez, Maha A. Alharbi, Diaa Massoud and Ehab Kotb Elmahallawy

Fermentation 2022, 8(8), 390; https://doi.org/10.3390/fermentation8080390 - 14 Aug 2022

Cited by 12 | Viewed by 2011

Abstract

Frozen yoghurt is a refreshing and nutritious dessert, with or without the flavour that combines the texture of ice cream and yoghurt. Several previous studies have been conducted on Jerusalem artichoke tubers due to their components, which contain inulin compounds and other nutrients [...] Read more.

Frozen yoghurt is a refreshing and nutritious dessert, with or without the flavour that combines the texture of ice cream and yoghurt. Several previous studies have been conducted on Jerusalem artichoke tubers due to their components, which contain inulin compounds and other nutrients with beneficial properties of fresh yoghurt. However, limited studies explored the potential benefits of the addition of Jerusalem artichoke tuber powder as a fat replacer on the physicochemical properties and survival of probiotics in frozen yoghurt. In this respect, the aim of this study was to determine the effect of Jerusalem artichoke tuber powder (JATP) (0, 5, 10, 15, and 20% w/w) of the fat source used in the mix as a fat, and sugar replacer in frozen yoghurt production. The microbiological, physicochemical, textural, and sensory properties of frozen yoghurt were investigated. Samples with JATP contained viable counts of bifidobacterium bifidum BGN4 and Lactobacillus casei Lc-01 of 7 log cfu/g during 90 days of storage, as compared to the control sample. The highest viability of probiotics was obtained in the sample formulated with 10% JATP. The formulation of frozen yoghurt with JATP increased the acidity and enhanced the overrun. Compared with the control sample, the incorporation of JATP into frozen yoghurt increased the melting resistance, overrun, and viscosity of the frozen yoghurt. The addition of JATP up to 10% significantly increased sensory attributes. Collectively, the study concluded that the enrichment of frozen yoghurt with JATP up to 20% will provide consumers with health benefits and could be introduced to markets as functional frozen yoghurt. Full article

(This article belongs to the Special Issue Dairy Fermentation)

13 pages, 1217 KiB

Open AccessArticle

Functional, Physicochemical, Rheological, Microbiological, and Organoleptic Properties of Synbiotic Ice Cream Produced from Camel Milk Using Black Rice Powder and Lactobacillus acidophilus LA-5

by Wael F. Elkot, Ateteallah H. Ateteallah, Maalem H. Al-Moalem, Magdy Ramadan Shahein, Mohamed A. Alblihed, Walied Abdo and Ehab Kotb Elmahallawy

Fermentation 2022, 8(4), 187; https://doi.org/10.3390/fermentation8040187 - 15 Apr 2022

Cited by 17 | Viewed by 3540

Abstract

Camel milk has become more popular among customers in recent years as a result of its therapeutic effects. In many parts of the world, it is considered one of the primary components of human nutrition. The present study aimed to develop a novel [...] Read more.

Camel milk has become more popular among customers in recent years as a result of its therapeutic effects. In many parts of the world, it is considered one of the primary components of human nutrition. The present study aimed to develop a novel synbiotic ice cream from camel milk formulated with black rice powder (BRP) and investigate the viability of probiotic bacteria (Lactobacillus acidophilus LA-5) during the storage period (60 days). Skim milk powder was replaced by BRP at levels of 0, 25, 50, and 75%. The produced ice cream was examined for some physicochemical, rheological, microbiological, and sensorial properties. The obtained results indicated that the incorporation of BRP into ice cream blends resulted in significant increases in the overrun, viscosity, and melting resistance of ice cream samples (p < 0.05). However, the freezing point decreased with increasing the proportion of BRP in the blend. The sensory evaluation results showed that the most acceptable treatments were those formulated with 25% and could be increased to 50% of BRP with no significant differences. The incorporation of BRP improved the viability of Lactobacillus acidophilus LA-5 in ice cream samples over 60 days of storage. Collectively, a synbiotic camel milk ice cream formulated with black rice powder was produced that, in turn, enhanced the physicochemical and rheological properties of ice cream samples and produced a significant protective effect on the viability of probiotic bacteria. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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Review

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22 pages, 417 KiB

Open AccessReview

Traditional Fermented Dairy Products in Southern Mediterranean Countries: From Tradition to Innovation

by Marina Mefleh, Amira M. Galal Darwish, Priti Mudgil, Sajid Maqsood and Fatma Boukid

Fermentation 2022, 8(12), 743; https://doi.org/10.3390/fermentation8120743 - 14 Dec 2022

Cited by 5 | Viewed by 4010

Abstract

Fermented dairy products have been essential elements in the diet of Southern Mediterranean countries for centuries. This review aims to provide an overview of the traditional fermented products in Southern Mediterranean countries, with a focus on fermented dairy products, and to discuss innovative [...] Read more.

Fermented dairy products have been essential elements in the diet of Southern Mediterranean countries for centuries. This review aims to provide an overview of the traditional fermented products in Southern Mediterranean countries, with a focus on fermented dairy products, and to discuss innovative strategies to make improved versions of these traditional products. A large variety of fermented dairy products were reviewed, showing high diversity, depending on the used raw materials, starter cultures, and preparation procedures. Traditionally, dairy products were fermented using spontaneous fermentation, back-slopping, and/or the addition of rennet. Compared with commercial products, traditional products are characterized by peculiar organoleptic features owing to the indigenous microflora. The main limitation of traditional products is preservation as most products were consumed fresh. In addition to drying, brine or oil was used to extend the product shelf life but resulted in high salt/fat products. Several studies suggested alternative ingredients/processing to make revised products with new flavors, improved nutritional quality, and a longer shelf life. There is still plenty of room for more research to obtain a better understanding of the indigenous microflora and on quality improvement and standardization to reach a wider market. Full article

(This article belongs to the Special Issue Dairy Fermentation)

0 pages, 1096 KiB

Open AccessReview

The Evolution of Fermented Milks, from Artisanal to Industrial Products: A Critical Review

by Thomas Bintsis and Photis Papademas

Fermentation 2022, 8(12), 679; https://doi.org/10.3390/fermentation8120679 - 27 Nov 2022

Cited by 15 | Viewed by 7062 | Correction

Abstract

The manufacture of fermented milk products has a long history, and these products were initially produced either from spontaneous fermentation or using a batch of previously produced product, that is, back-slopping. Milk of different mammal species has traditionally been used for the manufacture [...] Read more.

The manufacture of fermented milk products has a long history, and these products were initially produced either from spontaneous fermentation or using a batch of previously produced product, that is, back-slopping. Milk of different mammal species has traditionally been used for the manufacture of fermented milk products. Cow’s milk is the basis for most dairy fermented products around the world. Milk from other mammals, including sheep, goat, camel, mare, buffalo, and yak may have been historically more important and remain so in certain regions. The milks from different species have differences in chemical composition and in certain, vital for the fermentation, components. The diversity of fermented milk products is further influenced by the wide variety of manufacturing practices. A great number of fermented dairy products have been traditionally produced worldwide, and many of them are still produced either following the same traditional process or manufactured industrially, using standardized processes under controlled conditions with specified starter cultures. The evolution from traditional to industrial production, their specific regional differences, their special characteristics, and the microbiological aspects of fermented dairy products are discussed. Throughout the evolution of fermented milk products, functional and therapeutic properties have been attributed to certain components and thus, yogurts and fermented milks have gained a significant market share. These products have gained wide global recognition as they meet consumers’ expectations for health-promoting and functional foods. The exploitation of microbiological methods based on DNA (or RNA) extraction and recently high-throughput techniques allowed for the accurate identification of the microbiota of fermented milk products. These techniques have revealed the significance of the properties of the autochthonous microbes and provided novel insights into the role of the microbiota in the functional and organoleptic properties of many fermented milk products. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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10 pages, 5050 KiB

Open AccessReview

Microbial Communities in Home-Made and Commercial Kefir and Their Hypoglycemic Properties

by Birsen Yilmaz, Emine Elibol, H. Nakibapher Jones Shangpliang, Fatih Ozogul and Jyoti Prakash Tamang

Fermentation 2022, 8(11), 590; https://doi.org/10.3390/fermentation8110590 - 31 Oct 2022

Cited by 8 | Viewed by 3313

Abstract

Kefir is a popular traditional fermented dairy product in many countries. It has a complex and symbiotic culture made up of species of the genera Leuconostoc, Lactococcus, and Acetobacter, as well as Lactobacilluskefiranofaciens and Lentilactobacillus kefiri. Though kefir [...] Read more.

Kefir is a popular traditional fermented dairy product in many countries. It has a complex and symbiotic culture made up of species of the genera Leuconostoc, Lactococcus, and Acetobacter, as well as Lactobacilluskefiranofaciens and Lentilactobacillus kefiri. Though kefir has been commercialized in some countries, people are still traditionally preparing kefir at the household level. Kefir is known to have many nutritious values, where its consistent microbiota has been identified as the main valuable components of the product. Type 2 diabetes mellitus (T2DM) is a common diet-related disease and has been one of the main concerns in the world’s growing population. Kefir has been shown to have promising activities in T2DM, mostly via hypoglycemic properties. This review aims to explain the microbial composition of commercial and home-made kefir and its possible effects on T2DM. Some studies on animal models and human clinical trials have been reviewed to validate the hypoglycemic properties of kefir. Based on animal and human studies, it has been shown that consumption of kefir reduces blood glucose, improves insulin signaling, controls oxidative stress, and decreases progression of diabetic nephropathy. Moreover, probiotic bacteria such as lactic-acid bacteria and Bifidobacterium spp. and their end-metabolites in turn directly or indirectly help in controlling many gut disorders, which are also the main biomarkers in the T2DM condition and its possible treatment. Full article

(This article belongs to the Special Issue Dairy Fermentation)

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