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Open AccessArticle

Identification of Pseudo-nitzschia Cryptic Species Collected in the Gulf of Naples Using Whole-Cell Fluorescent In Situ Hybridization: From Cultured Sample to Field Test

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Diversity 2023, 15(4), 521; https://doi.org/10.3390/d15040521 - 04 Apr 2023

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Abstract

The planktonic diatom genus Pseudo-nitzschia contains several genetically closely related species that can produce domoic acid, a potent neurotoxin known to cause amnesic shellfish poisoning (ASP). An early identification and an adequate monitoring of the potential toxic Pseudo-nitzschia spp. are necessary. However, effective [...] Read more.

The planktonic diatom genus Pseudo-nitzschia contains several genetically closely related species that can produce domoic acid, a potent neurotoxin known to cause amnesic shellfish poisoning (ASP). An early identification and an adequate monitoring of the potential toxic Pseudo-nitzschia spp. are necessary. However, effective monitoring programs are time consuming due, in some cases, to the cell morphology similarities among species, determined with light microscopy, that can result in insufficient data to give a definitive species and toxins attribution. In this paper, Whole-Cell Fluorescent In Situ Hybridization (WC-FISH) has been evaluated as a powerful tool to detect and enumerate harmful cryptic and/or pseudo-cryptic Pseudo-nitzschia spp. collected in the Gulf of Naples. Fluorescently labelled probes directed against the ribosomal RNA (rRNA) of the 28S large subunit (LSU) were used. In particular, five probes detecting four cryptic species of Pseudo-nitzschia delicatissima complex and one specific for Pseudo-nitzschia multistriata gave good results for the molecular identification of potentially toxic target species in natural samples. Finally, we can state that the WC-FISH method, to identify Pseudo-nitzschia species, is faster and more cost-effective if compared with other rDNA-based methods. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

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Diversity 2023, 15(4), 504; https://doi.org/10.3390/d15040504 - 01 Apr 2023

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Abstract

One new species of the genus Dendronotus (Nudibranchia: Dendronotidae) is described from Norway and Northern Ireland, as well as from the adjacent North Sea, and one new subspecies of Dendronotus arcticus is described from Norway by applying a combination of fine-scale morphological and [...] Read more.

One new species of the genus Dendronotus (Nudibranchia: Dendronotidae) is described from Norway and Northern Ireland, as well as from the adjacent North Sea, and one new subspecies of Dendronotus arcticus is described from Norway by applying a combination of fine-scale morphological and molecular phylogenetic data. The present case demonstrates multilevel morphological and molecular similarities and differences considering on the one hand a grouping of three similar looking sympatric taxa (D. yrjargul, D. arcticus gartensis n. subsp. and D. keatleyae n. sp.), and on the other hand two different looking apparently allopatric subspecies (D. arcticus arcticus and D. arcticus gartensis n. subsp.). The type species of the genus, D. frondosus, which is the commonest dendronotid in Norway and the United Kingdom, consistently demonstrates substantial molecular and fine-scale morphological differences from D. keatleyae n. sp. The present study, apart from providing purely taxonomic information, also provides new data for a broad discussion of global biodiversity patterns. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

Assessing Biodiversity Conditions in Cocoa Agroforests with a Rapid Assessment Method: Outcomes from a Large-Scale Application in Ghana

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Sandra Oliveira

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Jessica E. Raneri

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Stephan F. Weise

Diversity 2023, 15(4), 503; https://doi.org/10.3390/d15040503 - 01 Apr 2023

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Abstract

Cocoa fields in West Africa traditionally kept other tree species to provide shade for cocoa trees and obtain food and other products. Measuring other trees is paramount to monitoring environmental conditions in cocoa agroforests, but it has been difficult to apply at a [...] Read more.

Cocoa fields in West Africa traditionally kept other tree species to provide shade for cocoa trees and obtain food and other products. Measuring other trees is paramount to monitoring environmental conditions in cocoa agroforests, but it has been difficult to apply at a large scale. This study presents the results of a rapid assessment method applied in Ghana, developed to measure non-cocoa tree characteristics based on easily observed parameters using sample surveys and mapping tools. We collected data from over 8700 cocoa farms and evaluated their biodiversity performance based on 6 indicators classified according to recommended thresholds to benefit biodiversity conditions. Our results show that species richness, shade cover, and potential for tree succession have the lowest proportions of fields with the recommended levels, with variations among regions and districts. The methodological procedure allowed us to identify priority areas and indicators falling behind desirable thresholds, which can inform training and management approaches regarding biodiversity-friendly practices in cocoa fields tailored to the needs of the farmers. The analysis procedure was developed with open-access automated routines, allowing for easy updates and replication to other areas, as well as for other commodities, enabling comparisons at different spatial scales and contributing to monitoring biodiversity over time. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

Economic Valuation of Northern White-Breasted Hedgehog Conservation

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Vasileios J. Kontsiotis

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Despina Chrysopoulou

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Parthena Chrysopoulou

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Vasilios Liordos

Diversity 2023, 15(4), 490; https://doi.org/10.3390/d15040490 - 27 Mar 2023

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Abstract

The northern white-breasted hedgehog (Erinaceus roumanicus) is a charismatic insectivore mammal threatened by habitat loss, the use of biocides, and collisions with vehicles. We aimed at valorizing hedgehog conservation through the contingent valuation method for estimating the proportion and the amount [...] Read more.

The northern white-breasted hedgehog (Erinaceus roumanicus) is a charismatic insectivore mammal threatened by habitat loss, the use of biocides, and collisions with vehicles. We aimed at valorizing hedgehog conservation through the contingent valuation method for estimating the proportion and the amount of willingness to pay (WTP) and the effects of cognitions and sociodemographic characteristics on WTP. We collected data through interviews with 704 residents of four administrative regions of northern Greece. Binary logistic regression and Welsh–Poe interval regression were used for estimating the effects of predictors on the proportion and mean WTP, respectively. Most participants (58.2%) were WTP a mean of EUR 31.7 for hedgehog conservation, totaling EUR 21.9 million annually when projected to the population. Positive attitudes, mutualism wildlife orientations, intention to participate in hedgehog conservation actions, and participation in consumptive and non-consumptive wildlife-related recreational activities increased WTP. Highly educated females, with high income and a pet were more WTP than less educated males, with low income and without a pet. Findings provided necessary information to managers about the value of hedgehog conservation, the potential for raising required funds and the differences in WTP among and within public groups. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

Revealing the Stygobiotic and Crenobiotic Molluscan Diversity in the Caucasus: Part IV—Crenobiotic Belgrandiellinae Radoman, 1983 (Mollusca, Hydrobiidae) from Georgia

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Aleksandra Jaszczyńska

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Andrzej Falniowski

Diversity 2023, 15(3), 450; https://doi.org/10.3390/d15030450 - 17 Mar 2023

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Abstract

Since 2020, the south-western Caucasus has been recognized as a hotspot of stygobiotic Mollusca diversity after revealing a large number of new, range-restricted species within the spring snail family Hydrobiidae, subfamily Sadlerianinae sensu Szarowska. Meantime, based on extensive material collected in the south-western [...] Read more.

Since 2020, the south-western Caucasus has been recognized as a hotspot of stygobiotic Mollusca diversity after revealing a large number of new, range-restricted species within the spring snail family Hydrobiidae, subfamily Sadlerianinae sensu Szarowska. Meantime, based on extensive material collected in the south-western Caucasus during the last decades, we studied members of another spring snail subfamily Belgrandiellinae Radoman, 1983. Modern integrative taxonomic work revealed hitherto unknown diversity within this subfamily in the region and further proved the importance of the south-western Caucasus as a hotspot of stygobiotic life. In particular, the subterranean environment and springs of Georgia were known to be inhabited by the genus Tschernomorica Vinarski and Palatov, 2019 with four nominal species. Our research, based on a morpho-anatomical study and genetic investigation of COI/H3 mitochondrial/nuclear markers, revealed additionally seven species and three genera new to science—Colchiella lugella gen. et sp. nov., C. nazodelavo gen. et sp. nov., C. shiksa gen. et sp. nov., C. dadiani gen. et sp. nov., Sataplia cavernicola gen. et sp. nov., Aetis starobogatovi gen. et sp. nov., and Tschernomorica kopidophora sp. nov. —to inhabit the Georgian part of south-western Caucasus. The full taxonomic description of each new taxa, along with the review of habitat characteristics and conservation status, is provided. Molecular genetics suggests that the ancestors of Caucasian Belgrandiellinae have migrated from south-western Europe, probably more than once during the late Messinian and early Pliocene periods. Later, Plio-Pleistocene climate oscillations, particularly the repeated rise and fall of the Black Sea water level, resulted in the isolation and radiation of various lineages within the Caucasus and Crimea. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

Biodiversity and Environmental Factors Structuring Diatom Assemblages of Mineral Saline Springs in the French Massif Central

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Diversity 2023, 15(2), 283; https://doi.org/10.3390/d15020283 - 15 Feb 2023

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Abstract

Springs are abundant and present worldwide and are among the most threatened ecosystems on Earth. The main aim of this study was to evaluate the biodiversity of seventy-nine (79) mineral saline springs situated in the French Massif Central, focusing on the species richness [...] Read more.

Springs are abundant and present worldwide and are among the most threatened ecosystems on Earth. The main aim of this study was to evaluate the biodiversity of seventy-nine (79) mineral saline springs situated in the French Massif Central, focusing on the species richness and the estimated richness and also on the diatom community composition. The influence of physical and chemical factors on both richness and communities was analyzed in order to have a better knowledge of the diatom ecological preferences and identify species typical of saline springs. Since December 2014, an on-going inventory of mineral springs has been in progress. For each spring, physical and chemical characteristics were measured, and benthic diatoms were sampled. The richness was the lowest in the springs presenting a man-made construction around the emergence. In the other springs, the highest richness was associated with the lowest lithium, sodium, total dissolved solid concentrations, and conductivity. Mineralization and some ions (bromine, calcium, chloride, fluoride, lithium, potassium, and sodium) were found to be the most critical drivers of diatom community composition. Some diatom species were typical of specific abiotic conditions, such as Navicula sanctamargaritae, which was associated with the highest potassium concentration. These species could appear as bio-indicators of these conditions. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessArticle

Euendolithic Infestation of Mussel Shells Indirectly Improves the Thermal Buffering Offered by Mussel Beds to Associated Molluscs, but One Size Does Not Fit All

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Alexia M. Dievart

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Christopher D. McQuaid

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Gerardo I. Zardi

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Katy R. Nicastro

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Pierre W. Froneman

Diversity 2023, 15(2), 239; https://doi.org/10.3390/d15020239 - 08 Feb 2023

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Abstract

Mussel beds form important intertidal matrices that provide thermal buffering to associated invertebrate communities, especially under stressful environmental conditions. Mussel shells are often colonized by photoautotrophic euendoliths, which have indirect conditional beneficial thermoregulatory effects on both solitary and aggregated mussels by increasing the [...] Read more.

Mussel beds form important intertidal matrices that provide thermal buffering to associated invertebrate communities, especially under stressful environmental conditions. Mussel shells are often colonized by photoautotrophic euendoliths, which have indirect conditional beneficial thermoregulatory effects on both solitary and aggregated mussels by increasing the albedo of the shell. We investigated whether euendolithic infestation of artificial mussel beds (Perna perna) influences the body temperatures of four associated mollusc species during simulated periods of emersion, using shell temperature obtained via non-invasive infrared thermography as a proxy. Shell temperatures of the limpet Scutellastra granularis and the chiton Acanthochitona garnoti were higher in non-infested than infested mussel beds during simulated low tides under high solar irradiance and low wind speeds. However, this was not the case for the limpet Helcion pectunculus or the top shell Oxystele antoni. Morphological differences in mollusc shape and colour could, in part, explain this contrast between species. Our results indicated that endolith-induced improvements in humidity and temperature in mussel beds could benefit associated molluscs. The beneficial thermal buffering offered by euendolithic infestation of the mussel beds was effective only if the organism was under heat stress. With global climate change, the indirect beneficial effect of euendolithic infestation for invertebrate communities associated with mussel beds may mitigate intertidal local extinction events triggered by marine heatwaves. Full article

(This article belongs to the Special Issue Diversity in 2023)

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Open AccessSystematic Review

Elephants Not in the Room: Systematic Review Shows Major Geographic Publication Bias in African Elephant Ecological Research

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Rachael B. Gross

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Robert Heinsohn

Diversity 2023, 15(3), 451; https://doi.org/10.3390/d15030451 - 17 Mar 2023

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Abstract

African savanna elephants (Loxodonta africana) are a keystone species in African ecosystems. As a result of increasing anthropogenic pressure, elephant populations have declined significantly in the last two centuries. Research on a broad sample of these populations is necessary to inform [...] Read more.

African savanna elephants (Loxodonta africana) are a keystone species in African ecosystems. As a result of increasing anthropogenic pressure, elephant populations have declined significantly in the last two centuries. Research on a broad sample of these populations is necessary to inform management strategies over a range of environmental and socio-political conditions. In order to evaluate the current state of literature that is informing evidence-based management and conservation of elephants, we systematically reviewed all research published on the ecology of African elephants from the last 20 years (492 publications). We contrasted the geographic distribution of published research against the 2016 IUCN elephant census. We found several statistically significant biases in the geographic distribution of elephant research. South Africa has 4.54% of the total elephant population and accounted for 28.28% of all research publications. Kenya has 5.49% the total elephant population but accounts for 20.6% of the research. Conversely, Botswana has 31.68% of the total elephant population but accounts for only 9.29% of the research and Zimbabwe has 19.89% of the total elephant population with only 10.50% of research. We also found that 41.85% of areas with ~60,100 elephants have not had any research published on their populations in the last 20 years. This publication imbalance may encourage management strategies that are overly dependent on misrepresentative information from a small subset of the elephant population. We recommend that (1) marginalised areas with large elephant populations (e.g., Botswana and Zimbabwe) should receive higher priority for future research, (2) new research and proposals should design theoretical frameworks to account for and overcome the present biases, and (3) local community-based management approaches should be prioritised and amplified in order to overcome the barriers to conducting research in priority areas. Full article

(This article belongs to the Special Issue Diversity in 2023)

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