Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
9.0
5.9
Journals
Nutrients
Special Issues
Beneficial Effects of Therapeutic Nutrition—New Insights from Advanced Cell Systems
nutrients-logo
Submit to Nutrients Review for Nutrients Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

Beneficial Effects of Therapeutic Nutrition—New Insights from Advanced Cell Systems

A special issue of Nutrients (ISSN 2072-6643). This special issue belongs to the section "Nutritional Immunology".

Deadline for manuscript submissions: closed (23 July 2023) | Viewed by 2827

Special Issue Editor

Dr. Tim Wolfs

E-Mail Website1 Website2
Guest Editor
Department of Paediatrics, Maastricht University, Maastricht, The Netherlands
Interests: fetal/neonatal development; immune modulation; pharmacological nutrition; plant sterols; cell-based therapies
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Advanced cell systems including organoids and organs-on-chips have shown the ability to recapitulate the function and physiology of organ systems, including the intestine. Accordingly, development of these models provides new opportunities to study the physiological responses of organ systems during health, disease and development. In addition, such advanced cell systems have been successfully employed to test pharmaceutical interventions.

Within this context, organoids and organ-on-chip models are increasingly used to test, among others, the uptake, transport and metabolism of nutritional components and to study their role in the prevention and maintenance of intestinal health.

This Special Issue welcomes original laboratory works, clinical studies, drug trials, case studies and (systematic) reviews focusing on advanced cellular systems in the context of nutritional research.

Potential topics include, but are not limited to, the following:

Cell systems, nutritional interventions and intestinal barrier loss, malabsorption, immune responses, responses of the intestinal epithelium and the influence of pathogenic stimuli.

Dr. Tim Wolfs
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nutrients is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nutraceuticals
  • immune responses
  • immune modulation
  • pharmacological nutrition
  • plant sterols
  • cell systems
  • intestinal development and injury
  • nutritional interventions
  • malabsorption

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

33 pages, 22093 KiB  
Article
Hypoxia-Driven Changes in a Human Intestinal Organoid Model and the Protective Effects of Hydrolyzed Whey
by Ilse H. de Lange, Charlotte van Gorp, Kimberly R. I. Massy, Lilian Kessels, Nico Kloosterboer, Ann Bjørnshave, Marie Stampe Ostenfeld, Jan G. M. C. Damoiseaux, Joep P. M. Derikx, Wim G. van Gemert and Tim G. A. M. Wolfs
Nutrients 2023, 15(2), 393; https://doi.org/10.3390/nu15020393 - 12 Jan 2023
Cited by 1 | Viewed by 2521
Abstract
Many whey proteins, peptides and protein-derived amino acids have been suggested to improve gut health through their anti-oxidant, anti-microbial, barrier-protective and immune-modulating effects. Interestingly, although the degree of hydrolysis influences peptide composition and, thereby, biological function, this important aspect is often overlooked. In [...] Read more.
Many whey proteins, peptides and protein-derived amino acids have been suggested to improve gut health through their anti-oxidant, anti-microbial, barrier-protective and immune-modulating effects. Interestingly, although the degree of hydrolysis influences peptide composition and, thereby, biological function, this important aspect is often overlooked. In the current study, we aimed to investigate the effects of whey protein fractions with different degrees of enzymatic hydrolysis on the intestinal epithelium in health and disease with a novel 2D human intestinal organoid (HIO) monolayer model. In addition, we aimed to assess the anti-microbial activity and immune effects of the whey protein fractions. Human intestinal organoids were cultured from adult small intestines, and a model enabling apical administration of nutritional components during hypoxia-induced intestinal inflammation and normoxia (control) in crypt-like and villus-like HIO was established. Subsequently, the potential beneficial effects of whey protein isolate (WPI) and two whey protein hydrolysates with a 27.7% degree of hydrolysis (DH28) and a 50.9% degree of hydrolysis (DH51) were assessed. In addition, possible immune modulatory effects on human peripheral immune cells and anti-microbial activity on four microbial strains of the whey protein fractions were investigated. Exposure to DH28 prevented paracellular barrier loss of crypt-like HIO following hypoxia-induced intestinal inflammation with a concomitant decrease in hypoxia inducible factor 1 alpha (HIF1α) mRNA expression. WPI increased Treg numbers and Treg expression of cluster of differentiation 25 (CD25) and CD69 and reduced CD4+ T cell proliferation, whereas no anti-microbial effects were observed. The observed biological effects were differentially mediated by diverse whey protein fractions, indicating that (degree of) hydrolysis influences their biological effects. Moreover, these new insights may provide opportunities to improve immune tolerance and promote intestinal health. Full article
(This article belongs to the Special Issue Beneficial Effects of Therapeutic Nutrition—New Insights from Advanced Cell Systems)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop