Foodborne Pathogens: Detection Technology and Safety Control

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 1106

Special Issue Editors


E-Mail Website
Guest Editor
College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China
Interests: microbial safety of food; foodborne pathogen control; rapid detection technology; microbial biofilm control; microbial drug resistance; stress response in pathogen

E-Mail Website
Guest Editor
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Interests: zoonotic bacterial pathogen control; foodborne pathogen control; bacteriophage therapy; microbial drug resistance; bacterial vaccine

Special Issue Information

Dear Colleagues,

Foodborne illness is one of the most prominent public health problems in the world, according to statistics, and approximately two-thirds of cases are caused by foodborne pathogens. Currently, the gold standard for the detection of foodborne pathogens is based on laboratory culture and biochemical experiments, but it is time-consuming and labor-intensive. To meet the urgent rapid response needs in food safety, it is crucial to develop rapid, sensitive, and high-throughput detection technology and products for foodborne pathogens.

The area of "detection technology of foodborne pathogens" has been a research hotspot in food safety in recent years, and scientists have conducted active research and innovation, mainly focusing on the establishment of various rapid detection technologies, the improved sensitivity and specificity of detection, the high-throughput detection of multiple pathogens, non-targeted screening based on fingerprint features of multi-omics, the rapid enrichment of pathogens in food, as well as microbial safety control for microbial biofilm and drug resistance.

This Special Issue aims to provide the latest research developments in this field in order to promote development of detection products and the application of deletion technology in the food industry.

Potential topics include, but are not limited to, the following:

  • The preservation and control of foodborne pathogens;
  • Animal source foodborn pathogen;
  • Nucleic-acid-based detection technology (PCR, RT-PCR, ddPCR, LAMP, RPA, molecular typing, etc.);
  • CRISPR/Cas-based detection technology;
  • Immunological detection techniques;
  • Biosensors for the detection of pathogens;
  • Biochips for high-throughput detection;
  • Non-targeted screening technology;
  • Aptamer detection technique;

  • Phage food sanitiser;
  • The detection of VBNC bacteria;
  • The development of portable and on-site testing kits;
  • Rapid enrichment strategies for pathogens;
  • The detection and detoxification of mycotoxins;
  • Microbial biofilm control;
  • Microbial drug resistance and food safety;
  • The prediction mode of microbial growth.

Prof. Dr. Xiaomei Bie
Prof. Dr. Wei Zhang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • foodborne pathogen
  • rapid detection technology
  • immunological detection
  • biosensor detection
  • biochips detection
  • non-targeted screening
  • aptamer detection
  • VBNC bacteria
  • rapid enrichment
  • microbial biofilm
  • microbial drug resistance
  • bacteriophage

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

15 pages, 5116 KiB  
Article
Investigating the Role of OrbF in Biofilm Biosynthesis and Regulation of Biofilm-Associated Genes in Bacillus cereus BC1
by Yang Sun, Wenjing Shuai, Lanmengya Nie, Xiangfei Li and Ling Jiang
Foods 2024, 13(5), 638; https://doi.org/10.3390/foods13050638 - 20 Feb 2024
Viewed by 879
Abstract
Bacillus cereus (B. cereus), a prevalent foodborne pathogen, constitutes a substantial risk to food safety due to its pronounced resilience under adverse environmental conditions such as elevated temperatures and ultraviolet radiation. This resilience can be attributed to its capacity for biofilm [...] Read more.
Bacillus cereus (B. cereus), a prevalent foodborne pathogen, constitutes a substantial risk to food safety due to its pronounced resilience under adverse environmental conditions such as elevated temperatures and ultraviolet radiation. This resilience can be attributed to its capacity for biofilm synthesis and sustained high viability. Our research aimed to elucidate the mechanisms governing biofilm biosynthesis in B. cereus. To this end, we constructed a 5088-mutant library of the B. cereus strain BC1 utilizing the transposon TnYLB-1. Systematic screening of this library yielded mutants exhibiting diminished biofilm formation capabilities. Twenty-four genes associated with the biofilm synthesis were identified by reverse PCR in these mutants, notably revealing a significant reduction in biofilm synthesis upon disruption of the orbF gene in B. cereus BC1. Comparative analysis between the wild type and orbF-deficient BC1 strains (BC1ΔorbF) indicated a marked downregulation (decreased by 11.7% to 96.7%) in the expression of genes implicated in biofilm formation, flagellar assembly, and bacterial chemotaxis in the BC1ΔorbF. Electrophoretic mobility shift assay (EMSA) further corroborated the role of OrbF, demonstrating its binding to the promoter region of the biofilm gene cluster, subsequently leading to the suppression of transcriptional activity of biofilm-associated genes in B. cereus BC1. Our findings underscore the pivotal role of orbF in biofilm biosynthesis in B. cereus, highlighting its potential as a target for strategies aimed at mitigating biofilm formation in this pathogen. Full article
(This article belongs to the Special Issue Foodborne Pathogens: Detection Technology and Safety Control)
Show Figures

Figure 1

Back to TopTop