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Development of FTIR-ATR, MALDI-TOF MS and Raman Based Method for...
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Development of FTIR-ATR, MALDI-TOF MS and Raman Based Method for Rapid Detection of Foodborne Bacteria

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: closed (20 March 2021) | Viewed by 2773

Special Issue Editor

Dr. Clara Sousa

E-Mail Website
Guest Editor
Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
Interests: vibrational spectroscopy; mass spectrometry; metabolomics; chemometrics; food analysis; plant characterization; antioxidants
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Foodborne bacteria represent a major public health issue even in industrialized countries. The identification/discrimination of such bacteria is crucial for epidemiological investigation and control of foodborne outbreaks. Several typing methods, such as polymerase chain reaction (PCR); sequencing of species-specific DNA regions; multilocus sequence typing (MLST); pulsed-field gel electrophoresis (PFGE) and, more recently, whole-genome sequence (WGS); are commonly employed for bacterial typing proposes. Despite the high accuracy of such techniques, they are time-consuming, laborious, and expensive. In the last few years, spectroscopic techniques have been used as reliable alternatives for bacterial typing at several taxonomic levels. Mass (MALDI-TOF MS) and vibrational (Raman and infrared) spectroscopic tecnhiques are among the most explored for such purposes due to their low cost, speed, and effectiveness.

Dr. Clara Sousa
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Foodborne bacteria
  • Mass spectroscopy
  • Infrared spectroscopy
  • Raman spectroscopy
  • Chemometrics

Published Papers (1 paper)

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Research

16 pages, 3668 KiB  
Article
Rapid Detection of Salmonella Enteritidis, Typhimurium, and Thompson by Specific Peak Analysis Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
by Seung-Min Yang, Eiseul Kim, Dayoung Kim, Jiwon Baek, Hyunjin Yoon and Hae-Yeong Kim
Foods 2021, 10(5), 933; https://doi.org/10.3390/foods10050933 - 23 Apr 2021
Cited by 13 | Viewed by 2332
Abstract
Rapid detection of Salmonella serovars is important for the effective control and monitoring of food industries. In this study, we evaluate the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the rapid detection of three serovars, Enteritidis, Typhimurium, and Thompson, that are [...] Read more.
Rapid detection of Salmonella serovars is important for the effective control and monitoring of food industries. In this study, we evaluate the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the rapid detection of three serovars, Enteritidis, Typhimurium, and Thompson, that are epidemiologically important in Korea. All strains were identified at the genus level, with a mean score of 2.319 using the BioTyper database, and their protein patterns were confirmed to be similar by principal component analysis and main spectrum profile dendrograms. Specific peaks for the three serovars were identified by analyzing 65 reference strains representing 56 different serovars. Specific mass peaks at 3018 ± 1 and 6037 ± 1, 7184 ± 1, and 4925 ± 1 m/z were uniquely found in the reference strains of serovars Enteritidis, Typhimurium, and Thompson, respectively, and they showed that the three serovars can be differentiated from each other and 53 other serovars. We verified the reproducibility of these mass peaks in 132 isolates, and serovar classification was achieved with 100% accuracy when compared with conventional serotyping through antisera agglutination. Our method can rapidly detect a large number of strains; hence, it will be useful for the high-throughput screening of Salmonella serovars. Full article
(This article belongs to the Special Issue Development of FTIR-ATR, MALDI-TOF MS and Raman Based Method for Rapid Detection of Foodborne Bacteria)
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