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DNA-Based Techniques for Authentication of Processed Food and Food Supplements
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DNA-Based Techniques for Authentication of Processed Food and Food Supplements

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Engineering and Technology".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 2358

Special Issue Editors

Dr. Junfeng Xu

E-Mail Website
Guest Editor
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Interests: DNA extraction from food samples; molecular markers; qPCR technology; rapid detection; GMO detection; safety assessment
Dr. Cheng Peng

E-Mail Website
Guest Editor
Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Interests: molecular detection; sequencing technology; traceability of GMOs; detection of gene-editing plants and its derived food; DNA fingerprinting
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food safety is of great significance to the social economy and stability in the lives of individuals. In food science and technology, one of the most important challenges is the authentication of processed food and food supplements.

In this Special Issue, we encourage submissions of review or research manuscripts related to the authentication of processed food and food supplements in the areas of food science and food safety, such as DNA extraction from food samples, molecular markers, food authenticity tests, PCR-based technology and transgenic food product tests. We are highly interested in and encourage manuscripts related to new techniques in this field for food safety, such as biosensors, DNA chips, high-throughput sequencing, etc.

Dr. Junfeng Xu
Dr. Cheng Peng
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • DNA extraction
  • molecular markers
  • qPCR technology
  • GM food detection
  • rapid and accurate detection
  • sequencing technology
  • DNA fingerprinting
  • food products authentication
  • grain dry technology
  • machine learning for food

Published Papers (2 papers)

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Research

17 pages, 1940 KiB  
Article
Specificity Testing for NGT PCR-Based Detection Methods in the Context of the EU GMO Regulations
by Caroline Bedin Zanatta, Aline Martins Hoepers, Rubens Onofre Nodari and Sarah Zanon Agapito-Tenfen
Foods 2023, 12(23), 4298; https://doi.org/10.3390/foods12234298 - 28 Nov 2023
Viewed by 1005
Abstract
The term new genomic techniques (NGTs) is an umbrella term used to describe a variety of techniques that can alter the genetic material of an organism and that have emerged or have been developed since 2001, when the existing genetically modified organism (GMO) [...] Read more.
The term new genomic techniques (NGTs) is an umbrella term used to describe a variety of techniques that can alter the genetic material of an organism and that have emerged or have been developed since 2001, when the existing genetically modified organism (GMO) legislation was adopted. The analytical framework used to detect GMOs in Europe is an established single harmonized procedure that is mandatory for the authorization of GM food and feed, thus generating a reliable, transparent, and effective labeling scheme for GMO products. However, NGT products can challenge the implementation and enforcement of the current regulatory system in the EU, relating in particular to the detection of NGT products that contain no foreign genetic material. Consequently, the current detection methods might fail to meet the minimum performance requirements. Although existing detection methods may be able to detect and quantify even small alterations in the genome, this does not necessarily confirm the distinction between products resulting from NGTs subject to the GMO legislation and other products. Therefore, this study provides a stepwise approach for the in silico prediction of PCR systems’ specificity by testing a bioinformatics pipeline for amplicon and primer set searches in current genomic databases. In addition, it also empirically tested the PCR system evaluated during the in silico analysis. Two mutant genotypes produced by CRISPR-Cas9 in Arabidopsis thaliana were used as a case study. Overall, our results demonstrate that the single PCR system developed for identifying a nucleotide insertion in the grf1-3 genotype has multiple matches in the databases, which do not enable the discrimination of this mutated event. Empirical assays further support this demonstration. In contrast, the second mutated genotype, grf8-61, which contains a -3 bp deletion, did not yield any matches in the sequence variant database. However, the primer sequences were not efficient during the empirical assay. Our approach represents a first step in decision making for analytical methods for NGT detection, identification, and quantification in light of the European labeling regulations. Full article
(This article belongs to the Special Issue DNA-Based Techniques for Authentication of Processed Food and Food Supplements)
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13 pages, 2916 KiB  
Article
Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods
by Lin Ding, Xiaoli Xu, Xiaofu Wang, Xiaoyun Chen, Yuwen Lu, Junfeng Xu and Cheng Peng
Foods 2023, 12(19), 3681; https://doi.org/10.3390/foods12193681 - 07 Oct 2023
Viewed by 891
Abstract
Effective regulation of gene-edited products and resolution of public concerns are the prerequisites for the industrialization of gene-edited crops and their derived foods. CRISPR-associated protein, the core element of the CRISPR system, requires to be regulated. Thus, there is an urgent need to [...] Read more.
Effective regulation of gene-edited products and resolution of public concerns are the prerequisites for the industrialization of gene-edited crops and their derived foods. CRISPR-associated protein, the core element of the CRISPR system, requires to be regulated. Thus, there is an urgent need to establish qualitative and quantitative detection methods for the Cas gene. In the present study, the primers and probes were designed and screened for Cas12a (Cpf1), which is the most commonly used target site in gene editing; we performed PCR system optimization, determined the optimal primer concentration and annealing temperature, and established qualitative PCR and quantitative PCR (qPCR) assays for detecting Cpf1 in gene editing by specificity and sensitivity tests. In specificity testing, qualitative PCR and qPCR methods could 100% detect samples containing Cpf1 DNA, while the detection rate of other samples without Cpf1 was 0%. In the assay sensitivity test, the limit of detection of qualitative PCR was 0.1% (approximately 44 copies), and the limit of detection of the qPCR method was 14 copies. In the stability test, both the qualitative PCR and qPCR methods were repeated 60 times at their corresponding lowest detection limit concentrations, and the results were positive. Thus, the qualitative and quantitative assays for Cpf1 are specific, sensitive, and stable. The method provides technical support for the effective monitoring of gene-edited products and their derived foods in the future. Full article
(This article belongs to the Special Issue DNA-Based Techniques for Authentication of Processed Food and Food Supplements)
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