Research of Metabolomics and Active Principle in Medicinal Plants

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Plant Metabolism".

Deadline for manuscript submissions: closed (1 May 2023) | Viewed by 3610

Special Issue Editors


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Guest Editor
Centroflora Inova - Centre of Research Development & Innovation, Campinas CEP 13069-380, SP, Brazil
Interests: metabolomics; natural products; medicinal plants; abiotic and biotic stress
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Guest Editor
Institute of Biosciences, University of São Paulo (USP), São Paulo 05508-060, Brazil
Interests: ecophysiology; climate change; natural products; molecular biology

Special Issue Information

Dear Colleagues,

Recent studies on plant-based natural products have brought sophisticated results that have established the basis for modern traditional medicine, and today, plants remain an essential source of active compounds. Apart from human use, specialized metabolites play important ecophysiological roles in the complex plant–environment relationship. Biotic and abiotic factors affect the biosynthesis of a wide range of specialized metabolites. For medicinal exploitation purposes, it is, therefore, important to identify the different (biotic, abiotic, and seasonal) factors that may affect the production and accumulation of specialized metabolites in different populations of species. From a methodological point of view, the diversity of chemical structures brings a great analytical challenge. Owing to analytical and computational advancements, the metabolomic approach has proven to be an appropriate way to assess such complex interactions, without the need for compound isolation. On the other hand, the isolation of compounds is necessary to determine the chemical structure of new natural products and to assess their bioactivity.

In this Special Issue, we expect to bring together works that use metabolomic approaches to advance the understanding of plant–environment interactions, the determination of chemical markers, and the prioritization and targeted isolation of active principles from medicinal plants. Metabolomic work performed in different analytical platforms such as LC–MS, GC–MS, and NMR, among others, will be considered.

Dr. Luiz Leonardo Saldanha
Dr. Fernanda Mendes De Rezende
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • natural products
  • biotic stress
  • abiotic stress
  • climate change
  • herbal medicine
  • metabolomic
  • molecular biology

Published Papers (2 papers)

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Research

24 pages, 7100 KiB  
Article
Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry
by Dan Rao, Ruoxi Zhao, Yadong Hu, Hongjie Li, Ze Chun and Shigang Zheng
Metabolites 2023, 13(6), 702; https://doi.org/10.3390/metabo13060702 - 28 May 2023
Cited by 1 | Viewed by 1533
Abstract
The medicinal plant Dendrobium nobile is an important natural antioxidant resource. To reveal the antioxidants of D. nobile, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for metabolic analysis. The H2O2-induced oxidative damage was used in human [...] Read more.
The medicinal plant Dendrobium nobile is an important natural antioxidant resource. To reveal the antioxidants of D. nobile, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for metabolic analysis. The H2O2-induced oxidative damage was used in human embryonic kidney 293T (H293T) cells to assess intracellular antioxidant activities. Cells incubated with flower and fruit extracts showed better cell survival, lower levels of reactive oxygen species (ROS), and higher catalase and superoxide dismutase activities than those incubated with root, stem, and leaf extracts (p < 0.01). A total of 13 compounds were newly identified as intracellular antioxidants by association analysis, including coniferin, galactinol, trehalose, beta-D-lactose, trigonelline, nicotinamide-N-oxide, shikimic acid, 5′-deoxy-5′-(methylthio)adenosine, salicylic acid, isorhamnetin-3-O-neohespeidoside, methylhesperidin, 4-hydroxybenzoic acid, and cis-aconitic acid (R2 > 0.8, Log2FC > 1, distribution > 0.1%, and p < 0.01). They showed lower molecular weight and higher polarity, compared to previously identified in vitro antioxidants in D. nobile (p < 0.01). The credibility of HPLC-MS/MS relative quantification was verified by common methods. In conclusion, some saccharides and phenols with low molecular weight and high polarity helped protect H293T cells from oxidative damage by increasing the activities of intracellular antioxidant enzymes and reducing intracellular ROS levels. The results enriched the database of safe and effective intracellular antioxidants in medicinal plants. Full article
(This article belongs to the Special Issue Research of Metabolomics and Active Principle in Medicinal Plants)
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19 pages, 3543 KiB  
Article
NMR-Based Metabolomics Reveals Effects of Water Stress in the Primary and Specialized Metabolisms of Bauhinia ungulata L. (Fabaceae)
by Ana Júlia Borim de Souza, Fernanda Maria Marins Ocampos, Rafael Catoia Pulgrossi, Anne Lígia Dokkedal, Luiz Alberto Colnago, Inês Cechin and Luiz Leonardo Saldanha
Metabolites 2023, 13(3), 381; https://doi.org/10.3390/metabo13030381 - 3 Mar 2023
Cited by 4 | Viewed by 1375
Abstract
Bauhinia ungulata is a plant used in Brazilian traditional medicine for the treatment of diabetes. Phytochemical studies revealed flavonoids and the saccharide pinitol related to hypoglycemic activity of the Bauhinia species. To determine the effects of water deficit on ecophysiological parameter and metabolite [...] Read more.
Bauhinia ungulata is a plant used in Brazilian traditional medicine for the treatment of diabetes. Phytochemical studies revealed flavonoids and the saccharide pinitol related to hypoglycemic activity of the Bauhinia species. To determine the effects of water deficit on ecophysiological parameter and metabolite fingerprints of B. ungulata, specimens were treated with the following water regimens under greenhouse conditions: daily watering (control), watering every 7 days (group 7D), and watering every 15 days (group 15D). Metabolite profiling of the plants subjected to water deficit was determined by LC-HRMS/MS. An NMR-based metabolomics approach applied to analyze the extracts revealed increased levels of known osmoprotective and bioactive compounds, such as D-pinitol, in the water deficit groups. Physiological parameters were determined by gas exchange in planta analysis. The results demonstrated a significant decrease in gas exchange under severe drought stress, while biomass production was not significantly different between the control and group 7D under moderate stress. Altogether, the results revealed that primary and specialized/secondary metabolism is affected by long periods of severe water scarcity downregulating the biosynthesis of bioactive metabolites such as pinitol, and the flavonoids quercetin and kaempferol. These results may be useful for guiding agricultural production and standardizing medicinal herb materials of this medicinal plant. Full article
(This article belongs to the Special Issue Research of Metabolomics and Active Principle in Medicinal Plants)
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