Submit to Special Issue Submit Abstract to Special Issue Review for IJMS Propose a Special Issue

Journal Menu

Journal Browser

Application of Biotechnology to Dental Treatment

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: 20 October 2024 | Viewed by 5410

Share This Special Issue

Special Issue Editors

Dr. Satoshi Komasa

E-Mail Website
Guest Editor

Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi 573-1121, Osaka, Japan
Interests: implant; titanium; zirconia; PEEK; HA coating; nano structure; bacteria
Special Issues, Collections and Topics in MDPI journals

Prof. Dr. Yoshiya Hashimoto

E-Mail Website
Guest Editor

Department of Biomaterials, Osaka Dental University, 8-1, Kuzuha Hanazono-cho, Hirakata 573-1121, Japan
Interests: periodontal tissue regeneration; IPS cells; environmental influence assessment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Recent advances in dental materials involving the development of various biomaterials have been reported. Accordingly, clinicians must incorporate the new dental technology in their practice to respond to the increasing needs of patients. In recent years, various biotechnologies have been developed and have had a great impact on the dental field. In addition to the development of new dental materials, the realization of regeneration technology using IPS cells is also expected. In this Special Issue, we invite the submission of several research papers on the development of biotechnology to dental treatment. In this general discussion, we briefly explain the relevant research reports, with the aim that developments in this field will contribute toward the development of dental care in the future.

Dr. Satoshi Komasa
Prof. Dr. Yoshiya Hashimoto
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

biotechnology
implant material
titanium
zirconia
PEEK
in vitro
in vivo

Published Papers (5 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

19 pages, 6749 KiB

Open AccessArticle

Effect of Er:YAG Pulsed Laser-Deposited Hydroxyapatite Film on Titanium Implants on M2 Macrophage Polarization In Vitro and Osteogenesis In Vivo

by Lin Ma, Min Li, Satoshi Komasa, Shigeki Hontsu, Yoshiya Hashimoto, Joji Okazaki and Kenji Maekawa

Int. J. Mol. Sci. 2024, 25(1), 349; https://doi.org/10.3390/ijms25010349 - 26 Dec 2023

Cited by 2 | Viewed by 808

Abstract

In a previous study, we successfully coated hydroxyapatite (HAp) onto titanium (Ti) plates using the erbium-doped yttrium aluminum garnet pulsed-laser deposition (Er:YAG-PLD) method. In this study, we performed further experiments to validate the in vitro osteogenic properties, macrophage polarization, and in vivo osseointegration activity of HAp-coated Ti (HAp-Ti) plates and screws. Briefly, we coated a HAp film onto the surfaces of Ti plates and screws via Er:YAG-PLD. The surface morphological, elemental, and crystallographic analyses confirmed the successful surface coating. The macrophage polarization and osteogenic induction were evaluated in macrophages and rat bone marrow mesenchymal stem cells, and the in vivo osteogenic properties were studied. The results showed that needle-shaped nano-HAp promoted the early expression of osteogenic and immunogenic genes in the macrophages and induced excellent M2 polarization properties. The calcium deposition and osteocalcin production were significantly higher in the HAp-Ti than in the uncoated Ti. The implantation into rat femurs revealed that the HAp-coated materials had superior osteoinductive and osseointegration activities compared with the Ti, as assessed by microcomputed tomography and histology. Thus, HAp film on sandblasted Ti plates and screws via Er:YAG-PLD enhances hard-tissue differentiation, macrophage polarization, and new bone formation in tissues surrounding implants both in vitro and in vivo. Full article

(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)

► Show Figures

Figure 1

14 pages, 4963 KiB

Open AccessArticle

Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment

by Yuzhu Sun, Jun-Ichiro Jo and Yoshiya Hashimoto

Int. J. Mol. Sci. 2023, 24(24), 17532; https://doi.org/10.3390/ijms242417532 - 15 Dec 2023

Viewed by 597

Abstract

This study aimed to develop a novel culture method for rat adipose-derived stem cells (rADSC) and evaluate their osteogenic potential. The rADSC cultured in xeno-free culture medium (XF-rADSCs) or conventional culture medium containing fetal bovine serum (FBS-rADSCs) were combined with micropieces of xeno-free recombinant collagen peptide to form 3-dimensional aggregates (XF-rADSC-CellSaic or FBS-rADSC-CellSaic). Both FBS-rADSC and XF-ADSC in CellSaic exhibited multilineage differentiation potential. Compared to FBS-rADSC-CellSaic, XF-rADSC-CellSaic accelerated and promoted osteogenic differentiation in vitro. When transplanted into rat mandibular congenital bone defects, the osteogenically differentiated XF-rADSC-CellSaic induced regeneration of bone tissue with a highly maturated structure compared to FBS-rADSC-CellSaic. In conclusion, XF-rADSC-CellSaic is a feasible 3-dimensional platform for efficient bone formation. Full article

(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)

► Show Figures

Figure 1

11 pages, 2536 KiB

Open AccessArticle

Novel Carboxylation Method for Polyetheretherketone (PEEK) Surface Modification Using Friedel–Crafts Acylation

by Xinghui Lyu, Ryuhei Kanda, Susumu Tsuda, Yoshiya Hashimoto, Takamasa Fujii and Kosuke Kashiwagi

Int. J. Mol. Sci. 2023, 24(21), 15651; https://doi.org/10.3390/ijms242115651 - 27 Oct 2023

Viewed by 849

Abstract

Recently, polyetheretherketone (PEEK) has shown promising dental applications. Surface treatment is essential for dental applications owing to its poor surface energy and wettability; however, no consensus on an effective treatment method has been achieved. In this study, we attempted to carboxylate PEEK sample surfaces via Friedel–Crafts acylation using succinic anhydride and AlBr₃. The possibility of further chemical modifications using carboxyl groups was examined. The samples were subjected to dehydration–condensation reactions with 1H,1H-pentadecafluorooctylamine and N,N’-dicyclohexylcarbodiimide. Furthermore, the sample’s surface properties at each reaction stage were evaluated. An absorption band in the 3300–3500 cm⁻¹ wavenumber region was observed. Additionally, peak suggestive of COOH was observed in the sample spectra. Secondary modification diminished the absorption band in 3300–3500 cm⁻¹ and a clear F1s signal was observed. Thus, Friedel–Crafts acylation with succinic anhydride produced carboxyl groups on the PEEK sample surfaces. Further chemical modification of the carboxyl groups by dehydration-condensation reactions is also possible. Thus, a series of reactions can be employed to impart desired chemical structures to PEEK surfaces. Full article

(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)

► Show Figures

Figure 1

31 pages, 16879 KiB

Open AccessArticle

In Situ Raman Study of Neurodegenerated Human Neuroblastoma Cells Exposed to Outer-Membrane Vesicles Isolated from Porphyromonas gingivalis

by Giuseppe Pezzotti, Tetsuya Adachi, Hayata Imamura, Davide Redolfi Bristol, Keiji Adachi, Toshiro Yamamoto, Narisato Kanamura, Elia Marin, Wenliang Zhu, Toshihisa Kawai, Osam Mazda, Toru Kariu, Tomonori Waku, Frank C. Nichols, Pietro Riello, Flavio Rizzolio, Tania Limongi and Kazu Okuma

Int. J. Mol. Sci. 2023, 24(17), 13351; https://doi.org/10.3390/ijms241713351 - 28 Aug 2023

Cited by 3 | Viewed by 1622

Abstract

The aim of this study was to elucidate the chemistry of cellular degeneration in human neuroblastoma cells upon exposure to outer-membrane vesicles (OMVs) produced by Porphyromonas gingivalis (Pg) oral bacteria by monitoring their metabolomic evolution using in situ Raman spectroscopy. Pg-OMVs are a key factor in Alzheimer’s disease (AD) pathogenesis, as they act as efficient vectors for the delivery of toxins promoting neuronal damage. However, the chemical mechanisms underlying the direct impact of Pg-OMVs on cell metabolites at the molecular scale still remain conspicuously unclear. A widely used in vitro model employing neuroblastoma SH-SY5Y cells (a sub-line of the SK-N-SH cell line) was spectroscopically analyzed in situ before and 6 h after Pg-OMV contamination. Concurrently, Raman characterizations were also performed on isolated Pg-OMVs, which included phosphorylated dihydroceramide (PDHC) lipids and lipopolysaccharide (LPS), the latter in turn being contaminated with a highly pathogenic class of cysteine proteases, a key factor in neuronal cell degradation. Raman characterizations located lipopolysaccharide fingerprints in the vesicle structure and unveiled so far unproved aspects of the chemistry behind protein degradation induced by Pg-OMV contamination of SH-SY5Y cells. The observed alterations of cells’ Raman profiles were then discussed in view of key factors including the formation of amyloid β (Aβ) plaques and hyperphosphorylated Tau neurofibrillary tangles, and the formation of cholesterol agglomerates that exacerbate AD pathologies. Full article

(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)

► Show Figures

Figure 1

15 pages, 26202 KiB

Open AccessArticle

Bioactive Polyetheretherketone with Gelatin Hydrogel Leads to Sustained Release of Bone Morphogenetic Protein-2 and Promotes Osteogenic Differentiation

by Ruonan Zhang, Jun-Ichiro Jo, Ryuhei Kanda, Aki Nishiura, Yoshiya Hashimoto and Naoyuki Matsumoto

Int. J. Mol. Sci. 2023, 24(16), 12741; https://doi.org/10.3390/ijms241612741 - 13 Aug 2023

Cited by 1 | Viewed by 1115

Abstract

Polyetheretherketone (PEEK) is one of the most promising implant materials for hard tissues due to its similar elastic modulus; however, usage of PEEK is still limited owing to its biological inertness and low osteoconductivity. The objective of the study was to provide PEEK with the ability to sustain the release of growth factors and the osteogenic differentiation of stem cells. The PEEK surface was sandblasted and modified with polydopamine (PDA). Moreover, successful sandblasting and PDA modification of the PEEK surface was confirmed through physicochemical characterization. The gelatin hydrogel was then chemically bound to the PEEK by adding a solution of glutaraldehyde and gelatin to the surface of the PDA-modified PEEK. The binding and degradation of the gelatin hydrogel with PEEK (GPEEK) were confirmed, and the GPEEK mineralization was observed in simulated body fluid. Sustained release of bone morphogenetic protein (BMP)-2 was observed in GPEEK. When cultured on GPEEK with BMP-2, human mesenchymal stem cells (hMSCs) exhibited osteogenic differentiation. We conclude that PEEK with a gelatin hydrogel incorporating BMP-2 is a promising substrate for bone tissue engineering. Full article

(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)

► Show Figures

Journal Menu

Journal Browser

Application of Biotechnology to Dental Treatment

Share This Special Issue

Special Issue Editors

Special Issue Information

Keywords

Published Papers (5 papers)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI