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Recent Advances in Fungal Secondary Metabolism, 2nd Edition
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Recent Advances in Fungal Secondary Metabolism, 2nd Edition

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Cell Biology, Metabolism and Physiology".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 1340

Special Issue Editors

Dr. Carlos García-Estrada

E-Mail Website
Guest Editor
Departamento de Ciencias Biomédicas, Facultad de Veterinaria, Universidad de León, León, Spain
Interests: fungal secondary metabolism; natural products; drug discovery
Special Issues, Collections and Topics in MDPI journals
Dr. Inmaculada Vaca

E-Mail Website
Guest Editor
Departamento de Química, Facultad de Ciencias, Universidad de Chile, Santiago, ‎Chile
Interests: fungal secondary metabolism; natural products; fungi from Antarctica
Special Issues, Collections and Topics in MDPI journals
Dr. Renato Chávez

E-Mail Website
Guest Editor
Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile
Interests: fungal secondary metabolism; fungal biology; fungi from Antarctica
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The fungi kingdom includes a diverse group of eukaryotic organisms able to produce a vast array of chemically diverse natural products known as secondary metabolites; these are crucial from a biotechnological point of view, and include antibiotics, immunosuppressants, pigments and mycotoxins, among others.

This Special Issue of Journal of Fungi invites researchers to contribute research articles, reviews, and opinions addressing relevant topics related to fungal secondary metabolism, including, but not limited to, the genetic analysis of biosynthetic gene clusters, the intra- and extracellular transport of secondary metabolites, regulatory circuits and transcription factors, epigenetic regulation, bioinformatics and genome mining, metabolic engineering and synthetic biology, the isolation and structural elucidation of novel fungal secondary metabolites, bioactivity assessment of pure natural products from fungi, and fungal metabolomics.

Dr. Carlos García-Estrada
Dr. Inmaculada Vaca
Dr. Renato Chávez
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • natural products
  • fungal secondary metabolism
  • Biosynthetic gene clusters (BGCs)
  • regulatory mechanisms
  • fungal biotechnology
  • metabolomics

Published Papers (1 paper)

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Research

16 pages, 2013 KiB  
Article
Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus
by Diego Palma, Vicente Oliva, Mariana Montanares, Carlos Gil-Durán, Dante Travisany, Renato Chávez and Inmaculada Vaca
J. Fungi 2024, 10(2), 157; https://doi.org/10.3390/jof10020157 - 16 Feb 2024
Viewed by 1044
Abstract
Fungi belonging to the genus Pseudogymnoascus have garnered increasing attention in recent years. One of the members of the genus, P. destructans, has been identified as the causal agent of a severe bat disease. Simultaneously, the knowledge of Pseudogymnoascus species has expanded, [...] Read more.
Fungi belonging to the genus Pseudogymnoascus have garnered increasing attention in recent years. One of the members of the genus, P. destructans, has been identified as the causal agent of a severe bat disease. Simultaneously, the knowledge of Pseudogymnoascus species has expanded, in parallel with the increased availability of genome sequences. Moreover, Pseudogymnoascus exhibits great potential as a producer of specialized metabolites, displaying a diverse array of biological activities. Despite these significant advancements, the genetic landscape of Pseudogymnoascus remains largely unexplored due to the scarcity of suitable molecular tools for genetic manipulation. In this study, we successfully implemented RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption in Pseudogymnoascus, using an Antarctic strain of Pseudogymnoascus verrucosus as a model. Both methods were applied to target azpA, a gene involved in red pigment biosynthesis. Silencing of the azpA gene to levels of 90% or higher eliminated red pigment production, resulting in transformants exhibiting a white phenotype. On the other hand, the CRISPR/Cas9 system led to a high percentage (73%) of transformants with a one-nucleotide insertion, thereby inactivating azpA and abolishing red pigment production, resulting in a white phenotype. The successful application of RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption represents a significant advancement in Pseudogymnoascus research, opening avenues for comprehensive functional genetic investigations within this underexplored fungal genus. Full article
(This article belongs to the Special Issue Recent Advances in Fungal Secondary Metabolism, 2nd Edition)
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