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New Applications of Natural Compounds as Functional Additives of Foods

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 891

Special Issue Editors


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Guest Editor

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Guest Editor
Department of Food Science and Nutrition, University of Thessaly, Terma N. Temponera Str., 43100 Karditsa, Greece
Interests: food lipids; food analysis; pulsed electric field; cloud point extraction; deep eutectic solvents; humic and fulvic acids
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Special Issue Information

Dear Colleagues,

It is unequivocally true that food consumption is not only vital for the maintenance of life, but also for well-being and the promotion of human health. However, for many centuries, food was produced with the sole purpose of satisfying hunger. In recent decades, due to extensive research carried out by various scientists, several compounds (mainly of plant origin) have been identified and further examined in terms of therapeutic applications. To this end, multiple ways to isolate compounds have been developed, resulting in multiple compounds with a wide variety of applications, either for prevention or for treatment. However, preparing and consuming natural extracts is a time-consuming and costly process for consumers, resulting in reduced interest. In this context, in order to overcome this issue, functional foods have been developed. Functional foods promote good health and assist in the prevention of diseases, making it a more appealing alternative option for consumers. Therefore, increasing effort is being dedicated to developing advanced functional foods. In addition to the production of functional foods, studies should focus on shedding light on the underlying mechanisms via which the desired therapeutic effect is achieved. In this context, nutraceuticals are often examined with advanced techniques to examine their effect on the microbiome and metabolome of the consumer and obtain a better insight into the underlying molecular mechanisms.

This Special Issue, titled “Natural Compounds as Functional Additives of Foods”, is dedicated to the advancements in this field, aiming (I) to further exploit the use of natural compounds that can serve as functions additives in foods and (II) to examine the mechanisms via which the desired effect is achieved. We invite authors to submit original research articles, communications, and reviews related to this topic.

Dr. Vassilis Athanasiadis
Dr. Theodoros G. Chatzimitakos
Prof. Dr. Stavros I. Lalas
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • antibacterial activity
  • anti-inflammatory activity
  • antioxidant activity
  • bioactive compounds
  • foodomics
  • functional food
  • green extraction techniques
  • health promotion
  • immunonutrition
  • metabolome alterations
  • microbiome
  • nutraceuticals
  • oxidative stress
  • polyphenols
  • vitamins

Published Papers (2 papers)

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Research

19 pages, 2836 KiB  
Article
Optimization of Pressurized Liquid Extraction (PLE) Parameters for Extraction of Bioactive Compounds from Moringa oleifera Leaves and Bioactivity Assessment
by Theodoros Chatzimitakos, Vassilis Athanasiadis, Konstantina Kotsou, Martha Mantiniotou, Dimitrios Kalompatsios, Ioannis Makrygiannis, Eleni Bozinou and Stavros I. Lalas
Int. J. Mol. Sci. 2024, 25(9), 4628; https://doi.org/10.3390/ijms25094628 - 24 Apr 2024
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Abstract
Moringa oleifera leaves are rich sources of bioactive compounds with potential health benefits, including antioxidants and anti-inflammatory agents. Pressurized liquid extraction (PLE) stands out as a promising technique for effectively extracting valuable compounds from natural sources. In this study, we aimed to optimize [...] Read more.
Moringa oleifera leaves are rich sources of bioactive compounds with potential health benefits, including antioxidants and anti-inflammatory agents. Pressurized liquid extraction (PLE) stands out as a promising technique for effectively extracting valuable compounds from natural sources. In this study, we aimed to optimize PLE parameters, such as temperature, extraction duration, and pressure, to maximize bioactive compound (polyphenols, flavonoids, and ascorbic acid) yield from M. oleifera leaves and evaluate their antioxidant and anti-inflammatory activities. According to the outcomes of this research, the maximum achieved total polyphenol content was 24.10 mg gallic acid equivalents (GAE)/g of dry weight (dw), and the total flavonoid content was increased up to 19.89 mg rutin equivalents (RtE)/g dw. Moreover, after HPLC-DAD analysis, neochlorogenic and chlorogenic acids, catechin and epicatechin, rutin, and narirutin were identified and quantified. As far as the optimum ascorbic acid content is concerned, it was found to be 4.77 mg/g dw. The antioxidant activity was evaluated by three different methods: ferric reducing antioxidant power (FRAP), the DPPH method, and the anti-hydrogen peroxide activity (AHPA) method, resulting in 124.29 μmol ascorbic acid equivalent (AAE)/g dw, 131.28 μmol AAE/g dw, and 229.38 μmol AAE/g dw values, respectively. Lastly, the albumin denaturation inhibition was found to be 37.54%. These findings underscore the potential of PLE as an efficient extraction method for preparing extracts from M. oleifera leaves with the maximum content of bioactive compounds. Full article
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20 pages, 5017 KiB  
Article
Isolation, Characterization, Genome Annotation, and Evaluation of Hyaluronidase Inhibitory Activity in Secondary Metabolites of Brevibacillus sp. JNUCC 41: A Comprehensive Analysis through Molecular Docking and Molecular Dynamics Simulation
by Yang Xu, Xuhui Liang and Chang-Gu Hyun
Int. J. Mol. Sci. 2024, 25(9), 4611; https://doi.org/10.3390/ijms25094611 - 23 Apr 2024
Viewed by 215
Abstract
Brevibacillus sp. JNUCC 41, characterized as a plant-growth-promoting rhizobacterium (PGPR), actively participates in lipid metabolism and biocontrol based on gene analysis. This study aimed to investigate the crucial secondary metabolites in biological metabolism; fermentation, extraction, and isolation were performed, revealing that methyl indole-3-acetate [...] Read more.
Brevibacillus sp. JNUCC 41, characterized as a plant-growth-promoting rhizobacterium (PGPR), actively participates in lipid metabolism and biocontrol based on gene analysis. This study aimed to investigate the crucial secondary metabolites in biological metabolism; fermentation, extraction, and isolation were performed, revealing that methyl indole-3-acetate showed the best hyaluronidase (HAase) inhibitory activity (IC50: 343.9 μM). Molecular docking results further revealed that the compound forms hydrogen bonds with the residues Tyr-75 and Tyr-247 of HAase (binding energy: −6.4 kcal/mol). Molecular dynamics (MD) simulations demonstrated that the compound predominantly binds to HAase via hydrogen bonding (MM-PBSA binding energy: −24.9 kcal/mol) and exhibits good stability. The residues Tyr-247 and Tyr-202, pivotal for binding in docking, were also confirmed via MD simulations. This study suggests that methyl indole-3-acetate holds potential applications in anti-inflammatory and anti-aging treatments. Full article
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