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22 pages, 3495 KiB

Open AccessArticle

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

by Zuzanna Garstecka, Marcel Antoszewski, Agnieszka Mierek-Adamska, Daniel Krauklis, Katarzyna Niedojadło, Beata Kaliska, Katarzyna Hrynkiewicz and Grażyna B. Dąbrowska

Int. J. Mol. Sci. 2023, 24(20), 15349; https://doi.org/10.3390/ijms242015349 - 19 Oct 2023

Cited by 1 | Viewed by 914

Abstract

In this work, we present the results of the inoculation of canola seeds (Brassica napus L.) with Trichoderma viride strains that promote the growth of plants. Seven morphologically different strains of T. viride (TvI-VII) were shown to be capable of synthesizing auxins [...] Read more.

In this work, we present the results of the inoculation of canola seeds (Brassica napus L.) with Trichoderma viride strains that promote the growth of plants. Seven morphologically different strains of T. viride (TvI-VII) were shown to be capable of synthesizing auxins and exhibited cellulolytic and pectinolytic activities. To gain a deeper insight into the molecular mechanisms underlying canola–T. viride interactions, we analyzed the canola stress genes metallothioneins (BnMT1-3) and stringent response genes (BnRSH1-3 and BnCRSH). We demonstrated the presence of cis-regulatory elements responsive to fungal elicitors in the promoter regions of B. napus MT and RSH genes and observed changes in the levels of the transcripts of the above-mentioned genes in response to root colonization by the tested fungal strains. Of the seven tested strains, under laboratory conditions, T. viride VII stimulated the formation of roots and the growth of canola seedlings to the greatest extent. An experiment conducted under field conditions during drought showed that the inoculation of canola seeds with a suspension of T. viride VII spores increased yield by 16.7%. There was also a positive effect of the fungus on the height and branching of the plants, the number of siliques, and the mass of a thousand seeds. We suggest that the T. viride strain TvVII can be used in modern sustainable agriculture as a bioinoculant and seed coating to protect B. napus from drought. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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17 pages, 3545 KiB

Open AccessArticle

A Novel Isoprene Synthase from the Monocot Tree Copernicia prunifera (Arecaceae) Confers Enhanced Drought Tolerance in Transgenic Arabidopsis

by Jiamei Yu, Iuliia Khomenko, Franco Biasioli, Mingai Li and Claudio Varotto

Int. J. Mol. Sci. 2023, 24(20), 15329; https://doi.org/10.3390/ijms242015329 - 18 Oct 2023

Viewed by 804

Abstract

The capacity to emit isoprene, among other stresses, protects plants from drought, but the molecular mechanisms underlying this trait are only partly understood. The Arecaceae (palms) constitute a very interesting model system to test the involvement of isoprene in enhancing drought tolerance, as [...] Read more.

The capacity to emit isoprene, among other stresses, protects plants from drought, but the molecular mechanisms underlying this trait are only partly understood. The Arecaceae (palms) constitute a very interesting model system to test the involvement of isoprene in enhancing drought tolerance, as their high isoprene emissions may have contributed to make them hyperdominant in neotropical dry forests, characterized by recurrent and extended periods of drought stress. In this study we isolated and functionally characterized a novel isoprene synthase, the gene responsible for isoprene biosynthesis, from Copernicia prunifera, a palm from seasonally dry tropical forests. When overexpressed in the non-emitter Arabidopsis thaliana, CprISPS conferred significant levels of isoprene emission, together with enhanced tolerance to water limitation throughout plant growth and development, from germination to maturity. CprISPS overexpressors displayed higher germination, cotyledon/leaf greening, water usage efficiency, and survival than WT Arabidopsis under various types of water limitation. This increased drought tolerance was accompanied by a marked transcriptional up-regulation of both ABA-dependent and ABA-independent key drought response genes. Taken together, these results demonstrate the capacity of CprISPS to enhance drought tolerance in Arabidopsis and suggest that isoprene emission could have evolved in Arecaceae as an adaptive mechanism against drought. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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16 pages, 4128 KiB

Open AccessArticle

Plasticity of Plantago lanceolata L. in Adaptation to Extreme Environmental Conditions

by Zbigniew Miszalski, Paweł Kaszycki, Marta Śliwa-Cebula, Adriana Kaczmarczyk, Miron Gieniec, Paulina Supel and Andrzej Kornaś

Int. J. Mol. Sci. 2023, 24(17), 13605; https://doi.org/10.3390/ijms241713605 - 02 Sep 2023

Viewed by 977

Abstract

This study aimed at characterizing some adaptive changes in Plantago lanceolata L. exposed to harsh conditions of a desert-like environment generating physiological stress of limited water availability and exposure to strong light. It was clearly shown that the plants were capable of adapting [...] Read more.

This study aimed at characterizing some adaptive changes in Plantago lanceolata L. exposed to harsh conditions of a desert-like environment generating physiological stress of limited water availability and exposure to strong light. It was clearly shown that the plants were capable of adapting their root system and vascular tissues to enable efficient vegetative performance. Soil analyses, as well as nitrogen isotope discrimination data show that P. lanceolata leaves in a desert-like environment had better access to nitrogen (nitrite/nitrate) and were able to fix it efficiently, as compared to the plants growing in the surrounding forest. The arbuscular mycorrhiza was also shown to be well-developed, and this was accompanied by higher bacterial frequency in the root zone, which might further stimulate plant growth. A closer look at the nitrogen content and leaf veins with a higher number of vessels and a greater vessel diameter made it possible to define the changes developed by the plants populating sandy habitats as compared with the vegetation sites located in the nearby forest. A determination of the photosynthesis parameters indicates that the photochemical apparatus in P. lanceolata inhabiting the desert areas adapted slightly to the desert-like environment and the time of day, with some changes of the reaction center (RC) size (photosystem II, PSII), while the plants’ photochemical activity was at a similar level. No differences between the two groups of plants were observed in the dissipation of light energy. The exposure of plants to harsh conditions of a desert-like environment increased the water use efficiency (WUE) value in parallel with possible stimulation of the β-carboxylation pathway. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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17 pages, 3440 KiB

Open AccessArticle

Hormesis Responses of Photosystem II in Arabidopsis thaliana under Water Deficit Stress

by Ilektra Sperdouli, Georgia Ouzounidou and Michael Moustakas

Int. J. Mol. Sci. 2023, 24(11), 9573; https://doi.org/10.3390/ijms24119573 - 31 May 2023

Cited by 6 | Viewed by 1073

Abstract

Since drought stress is one of the key risks for the future of agriculture, exploring the molecular mechanisms of photosynthetic responses to water deficit stress is, therefore, fundamental. By using chlorophyll fluorescence imaging analysis, we evaluated the responses of photosystem II (PSII) photochemistry [...] Read more.

Since drought stress is one of the key risks for the future of agriculture, exploring the molecular mechanisms of photosynthetic responses to water deficit stress is, therefore, fundamental. By using chlorophyll fluorescence imaging analysis, we evaluated the responses of photosystem II (PSII) photochemistry in young and mature leaves of Arabidopsis thaliana Col-0 (cv Columbia-0) at the onset of water deficit stress (OnWDS) and under mild water deficit stress (MiWDS) and moderate water deficit stress (MoWDS). Moreover, we tried to illuminate the underlying mechanisms in the differential response of PSII in young and mature leaves to water deficit stress in the model plant A. thaliana. Water deficit stress induced a hormetic dose response of PSII function in both leaf types. A U-shaped biphasic response curve of the effective quantum yield of PSII photochemistry (Φ_PSII) in A. thaliana young and mature leaves was observed, with an inhibition at MiWDS that was followed by an increase in Φ_PSII at MoWDS. Young leaves exhibited lower oxidative stress, evaluated by malondialdehyde (MDA), and higher levels of anthocyanin content compared to mature leaves under both MiWDS (+16%) and MoWDS (+20%). The higher Φ_PSII of young leaves resulted in a decreased quantum yield of non-regulated energy loss in PSII (Φ_NO), under both MiWDS (−13%) and MoWDS (−19%), compared to mature leaves. Since Φ_NO represents singlet-excited oxygen (¹O₂) generation, this decrease resulted in lower excess excitation energy at PSII, in young leaves under both MiWDS (−10%) and MoWDS (−23%), compared to mature leaves. The hormetic response of PSII function in both young and mature leaves is suggested to be triggered, under MiWDS, by the intensified reactive oxygen species (ROS) generation, which is considered to be beneficial for activating stress defense responses. This stress defense response that was induced at MiWDS triggered an acclimation response in A. thaliana young leaves and provided tolerance to PSII when water deficit stress became more severe (MoWDS). We concluded that the hormesis responses of PSII in A. thaliana under water deficit stress are regulated by the leaf developmental stage that modulates anthocyanin accumulation in a stress-dependent dose. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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11 pages, 1518 KiB

Open AccessArticle

Drought Resistance and Ginsenosides Biosynthesis in Response to Abscisic Acid in Panax ginseng C. A. Meyer

by Lingyao Kong, Peng Chen and Cheng Chang

Int. J. Mol. Sci. 2023, 24(11), 9194; https://doi.org/10.3390/ijms24119194 - 24 May 2023

Cited by 2 | Viewed by 1068

Abstract

Drought stress adversely affects the production of the perennial medicinal herb Panax ginseng C.A. Meyer. Phytohormone abscisic acid (ABA) regulates many processes in plant growth, development, and response to environments. However, whether drought resistance is regulated by ABA in Panax ginseng remains unknown. [...] Read more.

Drought stress adversely affects the production of the perennial medicinal herb Panax ginseng C.A. Meyer. Phytohormone abscisic acid (ABA) regulates many processes in plant growth, development, and response to environments. However, whether drought resistance is regulated by ABA in Panax ginseng remains unknown. In this study, we characterized the response of drought resistance to ABA in Panax ginseng. The results showed that the growth retardation and root shrinking under drought conditions in Panax ginseng were attenuated by exogenous ABA application. Spraying ABA was shown to protect the photosynthesis system, enhance the root activity, improve the performance of the antioxidant protection system, and alleviate the excessive accumulation of soluble sugar in Panax ginseng under drought stress. In addition, ABA treatment leads to the enhanced accumulation of ginsenosides, the pharmaceutically active components, and causes the up-regulation of 3-hydroxy-3-methylglutaryl CoA reductase (PgHMGR) in Panax ginseng. Therefore, this study supports that drought resistance and ginsenosides biosynthesis in Panax ginseng were positively regulated by ABA, providing a new direction for mitigating drought stress and improving ginsenosides production in the precious medicinal herb. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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21 pages, 6019 KiB

Open AccessArticle

Metabolomic Analysis of the Response of Haloxylon ammodendron and Haloxylon persicum to Drought

by Fang Yang and Guanghui Lv

Int. J. Mol. Sci. 2023, 24(10), 9099; https://doi.org/10.3390/ijms24109099 - 22 May 2023

Cited by 4 | Viewed by 1176

Abstract

Haloxylon ammodendron and Haloxylon persicum, as typical desert plants in arid areas, show strong drought tolerance and environmental adaptability and are therefore ideal model plants for studying the molecular mechanisms of drought tolerance. A metabolomic analysis of H. ammodendron and H. persicum [...] Read more.

Haloxylon ammodendron and Haloxylon persicum, as typical desert plants in arid areas, show strong drought tolerance and environmental adaptability and are therefore ideal model plants for studying the molecular mechanisms of drought tolerance. A metabolomic analysis of H. ammodendron and H. persicum in their natural environment is lacking, and their metabolic response to drought therefore remains unclear. To elucidate the response of H. ammodendron and H. persicum to drought at the metabolic level, a non-targeted metabolomics analysis was carried out herein. Under a dry environment, H. ammodendron exhibited 296 and 252 differentially expressed metabolites (DEMs) in the positive and negative ion modes, respectively, whereas 452 and 354 DEMs were identified in the positive and negative ion modes in H. persicum, respectively. The results indicated that H. ammodendron responds to drought by increasing the content of organic nitrogen compounds and lignans, neolignans, and related compounds, and reducing the content of alkaloids and derivatives. By contrast, H. persicum adapts to the dry environment by increasing the content of organic acids and their derivatives and reducing the content of lignans, neolignans, and related compounds. In addition, H. ammodendron and H. persicum improved their osmoregulation ability, reactive oxygen species detoxification ability, and cell membrane stability by regulating the key metabolic pathways and anabolism of associated metabolites. This is the first metabolomics report on the response of H. ammodendron and H. persicum to drought in their natural environment, providing a foundation for the further study of their regulatory mechanisms under drought stress. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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23 pages, 4337 KiB

Open AccessArticle

Network Biology Analyses and Dynamic Modeling of Gene Regulatory Networks under Drought Stress Reveal Major Transcriptional Regulators in Arabidopsis

by Nilesh Kumar, Bharat K. Mishra, Jinbao Liu, Binoop Mohan, Doni Thingujam, Karolina M. Pajerowska-Mukhtar and M. Shahid Mukhtar

Int. J. Mol. Sci. 2023, 24(8), 7349; https://doi.org/10.3390/ijms24087349 - 16 Apr 2023

Cited by 2 | Viewed by 2321

Abstract

Drought is one of the most serious abiotic stressors in the environment, restricting agricultural production by reducing plant growth, development, and productivity. To investigate such a complex and multifaceted stressor and its effects on plants, a systems biology-based approach is necessitated, entailing the [...] Read more.

Drought is one of the most serious abiotic stressors in the environment, restricting agricultural production by reducing plant growth, development, and productivity. To investigate such a complex and multifaceted stressor and its effects on plants, a systems biology-based approach is necessitated, entailing the generation of co-expression networks, identification of high-priority transcription factors (TFs), dynamic mathematical modeling, and computational simulations. Here, we studied a high-resolution drought transcriptome of Arabidopsis. We identified distinct temporal transcriptional signatures and demonstrated the involvement of specific biological pathways. Generation of a large-scale co-expression network followed by network centrality analyses identified 117 TFs that possess critical properties of hubs, bottlenecks, and high clustering coefficient nodes. Dynamic transcriptional regulatory modeling of integrated TF targets and transcriptome datasets uncovered major transcriptional events during the course of drought stress. Mathematical transcriptional simulations allowed us to ascertain the activation status of major TFs, as well as the transcriptional intensity and amplitude of their target genes. Finally, we validated our predictions by providing experimental evidence of gene expression under drought stress for a set of four TFs and their major target genes using qRT-PCR. Taken together, we provided a systems-level perspective on the dynamic transcriptional regulation during drought stress in Arabidopsis and uncovered numerous novel TFs that could potentially be used in future genetic crop engineering programs. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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19 pages, 7760 KiB

Open AccessArticle

Global Responses of Autopolyploid Sugarcane Badila (Saccharum officinarum L.) to Drought Stress Based on Comparative Transcriptome and Metabolome Profiling

by Shan Yang, Na Chu, Naijie Feng, Bolin Zhou, Hongkai Zhou, Zuhu Deng, Xuefeng Shen and Dianfeng Zheng

Int. J. Mol. Sci. 2023, 24(4), 3856; https://doi.org/10.3390/ijms24043856 - 14 Feb 2023

Cited by 4 | Viewed by 1593

Abstract

Sugarcane (Saccharum spp. hybrid) is frequently affected by seasonal drought, which causes substantial declines in quality and yield. To understand the drought resistance mechanisms of S. officinarum, the main species of modern sugarcane, at a molecular level, we carried out a [...] Read more.

Sugarcane (Saccharum spp. hybrid) is frequently affected by seasonal drought, which causes substantial declines in quality and yield. To understand the drought resistance mechanisms of S. officinarum, the main species of modern sugarcane, at a molecular level, we carried out a comparative analysis of transcriptome and metabolome profiling of the sugarcane variety Badila under drought stress (DS). Compared with control group (CG) plants, plants exposed to DS had 13,744 (6663 up-regulated and 7081 down-regulated) differentially expressed genes (DEGs). GO and KEGG analysis showed that the DEGs were enriched in photosynthesis-related pathways and most DEGs had down-regulated expression. Moreover, the chlorophyll content, photosynthesis (Photo), stomatal conductance (Cond), intercellular carbon dioxide concentration (Ci) and transpiration rate (Trmmol) were sharply decreased under DS. These results indicate that DS has a significant negative influence on photosynthesis in sugarcane. Metabolome analysis identified 166 (37 down-regulated and 129 up-regulated) significantly regulated metabolites (SRMs). Over 50% of SRMs were alkaloids, amino acids and their derivatives, and lipids. The five most significantly enriched KEGG pathways among SRMs were Aminoacyl-tRNA biosynthesis, 2-Oxocarboxylic acid metabolism, Biosynthesis of amino acids, Phenylalanine metabolism, and Arginine and proline metabolism (p < 0.05). Comparing CG with DS for transcriptome and metabolome profiling (T_CG/DS and M_CG/DS, respectively), we found three of the same KEGG-enriched pathways, namely Biosynthesis of amino acids, Phenylalanine metabolism and Arginine and proline metabolism. The potential importance of Phenylalanine metabolism and Arginine and proline metabolism was further analyzed for response to DS in sugarcane. Seven SRMs (five up-regulated and two down-regulated) and 60 DEGs (17 up-regulated and 43 down-regulated) were enriched in Phenylalanine metabolism under DS, of which novel.31261, Sspon.04G0008060-1A, Sspon.04G0008060-2B and Sspon.04G0008060-3C were significantly correlated with 7 SRMs. In Arginine and proline metabolism, eight SRMs (seven up-regulated and one down-regulated) and 63 DEGs (32 up-regulated and 31 down-regulated) were enriched, of which Sspon.01G0026110-1A (OAT) and Sspon.03G0002750-3D (P5CS) were strongly associated with proline (r > 0.99). These findings present the dynamic changes and possible molecular mechanisms of Phenylalanine metabolism as well as Arginine and proline metabolism under DS and provide a foundation for future research and sugarcane improvement. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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17 pages, 5698 KiB

Open AccessArticle

CBL-Interacting Protein Kinases 18 (CIPK18) Gene Positively Regulates Drought Resistance in Potato

by Liang Yang, Ning Zhang, Kaitong Wang, Zhiyong Zheng, Jingjing Wei and Huaijun Si

Int. J. Mol. Sci. 2023, 24(4), 3613; https://doi.org/10.3390/ijms24043613 - 10 Feb 2023

Cited by 4 | Viewed by 1761

Abstract

Sensor—responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) are plant-specific Ca²⁺ receptors, and the CBL-CIPK module is widely involved in plant growth and development and a large number of abiotic stress response signaling pathways. In this study, the [...] Read more.

Sensor—responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) are plant-specific Ca²⁺ receptors, and the CBL-CIPK module is widely involved in plant growth and development and a large number of abiotic stress response signaling pathways. In this study, the potato cv. “Atlantic” was subjected to a water deficiency treatment and the expression of StCIPK18 gene was detected by qRT-PCR. The subcellular localization of StCIPK18 protein was observed by a confocal laser scanning microscope. The StCIPK18 interacting protein was identified and verified by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). StCIPK18 overexpression and StCIPK18 knockout plants were constructed. The phenotypic changes under drought stress were indicated by water loss rate, relative water content, MDA and proline contents, and CAT, SOD and POD activities. The results showed that StCIPK18 expression was upregulated under drought stress. StCIPK18 is localized in the cell membrane and cytoplasm. Y2H shows the interaction between StCIPK18 and StCBL1, StCBL4, StCBL6 and StCBL8. BiFC further verifies the reliability of the interaction between StCIPK18 and StCBL4. Under drought stress, StCIPK18 overexpression decreased the water loss rate and MDA, and increased RWC, proline contents and CAT, SOD and POD activities; however, StCIPK18 knockout showed opposite results, compared with the wild type, in response to drought stress. The results can provide information for the molecular mechanism of the StCIPK18 regulating potato response to drought stress. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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24 pages, 19467 KiB

Open AccessArticle

Evolutionary Analysis of StSnRK2 Family Genes and Their Overexpression in Transgenic Tobacco Improve Drought Tolerance

by Panfeng Yao, Lei Sun, Simon Dekomah, Zhenzhen Bi, Chao Sun, Juan Mao, Chunli Zhang, Tianyuan Qin, Yihao Wang, Yuhui Liu, Zhen Liu, Kazim Ali and Jiangping Bai

Int. J. Mol. Sci. 2023, 24(2), 1000; https://doi.org/10.3390/ijms24021000 - 05 Jan 2023

Viewed by 1162

Abstract

Sucrose non-ferment 1-related protein kinase 2 (SnRK2) is a highly conserved protein kinase in plants that plays an important role in regulating plant response to drought stress. Although it has been reported in some plants, the evolutionary relationship of potato SnRK2s and their [...] Read more.

Sucrose non-ferment 1-related protein kinase 2 (SnRK2) is a highly conserved protein kinase in plants that plays an important role in regulating plant response to drought stress. Although it has been reported in some plants, the evolutionary relationship of potato SnRK2s and their function in drought resistance have not been systematically analyzed. In this study, molecular characteristic analysis showed that 8 StSnRK2s were distributed on six chromosomes, coding proteins were divided into three subgroups, and StSnRK2s clustered in the same subgroup had similar conserved motifs and domains. In addition, StSnRK2 has a wide range of replication events in some species, making it closer to dicots in the process of evolution. In addition, the average nonsynonymous substitution rate/synonymous substitution rate (Ka/Ks) value of SnRK2s in monocots was higher than that of dicots. The codon usage index showed that SnRK2s prefer to use cytosine 3 (C3s), guanine 3 (G3s) and GC content (GC3s) in monocots, whereas thymine 3 (T3s) and adenine 3 (A3s) are preferred in dicots. Furthermore, stress response analysis showed that the expression of StSnRK2s under different degrees of drought stress significantly correlated with one or more stress-related physiological indices, such as proline and malondialdehyde (MDA) content, superoxide dismutase (SOD) and catalase (CAT) activity, ion leakage (IL) etc. The drought resistance of StSnRK2 transgenic plants was determined to occur in the order of StSnRK2.1/2.8 > StSnRK2.2/2.5 > StSnRK2.4/2.6 > StSnRK2.3 > StSnRK2.7, was attributed to not only lower IL but also higher proline, soluble sugar contents and stress-related genes in transgenic plants compared to wild type (WT). In conclusion, this study provides useful insights into the evolution and function of StSnRK2s and lays a foundation for further study on the molecular mechanism of StSnRK2s regulating potato drought resistance. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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15 pages, 2331 KiB

Open AccessArticle

Differential Water Deficit in Leaves Is a Principal Factor Modifying Barley Response to Drought Stress

by Małgorzata Nykiel, Marta Gietler, Justyna Fidler, Jakub Graska, Anna Rybarczyk-Płońska, Beata Prabucka, Ewa Muszyńska, Jan Bocianowski and Mateusz Labudda

Int. J. Mol. Sci. 2022, 23(23), 15240; https://doi.org/10.3390/ijms232315240 - 03 Dec 2022

Cited by 5 | Viewed by 1249

Abstract

In response to environmental stress, plants activate complex signalling, including being dependent on reactive oxygen–nitrogen–sulphur species. One of the key abiotic stresses is drought. As a result of drought, changes in the level of hydration of the plant occur, which obviously entails various [...] Read more.

In response to environmental stress, plants activate complex signalling, including being dependent on reactive oxygen–nitrogen–sulphur species. One of the key abiotic stresses is drought. As a result of drought, changes in the level of hydration of the plant occur, which obviously entails various metabolic alternations. The primary aim of this study was to determine the relationship between the response of barley to drought and the intensity of stress, therefore investigations were performed under various levels of water saturation deficit (WSD) in leaves at 15%, 30%, and 50%. In barley subjected to drought, most significant changes occurred under a slight dehydration level at 15%. It was observed that the gene expression of 9-cis-epoxycarotenoid dioxygenases, enzymes involved in ABA biosynthesis, increased significantly, and led to a higher concentration of ABA. This was most likely the result of an increase in the gene expression and enzyme activity of L-cysteine desulfhydrase, which is responsible for H₂S synthesis. Our results suggest that the differential water deficit in leaves underlies the activation of an appropriate defence, with ABA metabolism at the centre of these processes. Furthermore, at 15% WSD, a dominant contribution of H₂O₂-dependent signalling was noted, but at 30% and 50% WSD, significant NO-dependent signalling occurred. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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16 pages, 5052 KiB

Open AccessArticle

Identification and Functional Analysis of bZIP Genes in Cotton Response to Drought Stress

by Boyang Zhang, Cheng Feng, Lin Chen, Baoqi Li, Xianlong Zhang and Xiyan Yang

Int. J. Mol. Sci. 2022, 23(23), 14894; https://doi.org/10.3390/ijms232314894 - 28 Nov 2022

Cited by 6 | Viewed by 1501

Abstract

The basic leucine zipper (bZIP) transcription factors, which harbor a conserved bZIP domain composed of two regions, a DNA-binding basic region and a Leu Zipper region, operate as important switches of transcription networks in eukaryotes. However, this gene family has not been systematically [...] Read more.

The basic leucine zipper (bZIP) transcription factors, which harbor a conserved bZIP domain composed of two regions, a DNA-binding basic region and a Leu Zipper region, operate as important switches of transcription networks in eukaryotes. However, this gene family has not been systematically characterized in cotton (Gossypium hirsutum). Here, we identified 197 bZIP family members in cotton. The chromosome distribution pattern indicates that the GhbZIP genes have undergone 53 genome-wide segmental and 7 tandem duplication events which contribute to the expansion of the cotton bZIP family. Phylogenetic analysis showed that cotton GhbZIP proteins cluster into 13 subfamilies, and homologous protein pairs showed similar characteristics. Inspection of the DNA-binding basic region and leucine repeat heptads within the bZIP domains indicated different DNA-binding site specificities as well as dimerization properties among different groups. Comprehensive expression analysis indicated the most highly and differentially expressed genes in root and leaf that might play significant roles in cotton response to drought stress. GhABF3D was identified as a highly and differentially expressed bZIP family gene in cotton leaf and root under drought stress treatments that likely controls drought stress responses in cotton. These data provide useful information for further functional analysis of the GhbZIP gene family and its potential application in crop improvement. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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18 pages, 14210 KiB

Open AccessArticle

Encapsulation Reduces the Deleterious Effects of Salicylic Acid Treatments on Root Growth and Gravitropic Response

by Jimmy Sampedro-Guerrero, Vicente Vives-Peris, Aurelio Gomez-Cadenas and Carolina Clausell-Terol

Int. J. Mol. Sci. 2022, 23(22), 14019; https://doi.org/10.3390/ijms232214019 - 14 Nov 2022

Cited by 4 | Viewed by 1741

Abstract

The role of salicylic acid (SA) on plant responses to biotic and abiotic stresses is well documented. However, the mechanism by which exogenous SA protects plants and its interactions with other phytohormones remains elusive. SA effect, both free and encapsulated (using silica and [...] Read more.

The role of salicylic acid (SA) on plant responses to biotic and abiotic stresses is well documented. However, the mechanism by which exogenous SA protects plants and its interactions with other phytohormones remains elusive. SA effect, both free and encapsulated (using silica and chitosan capsules), on Arabidopsis thaliana development was studied. The effect of SA on roots and rosettes was analysed, determining plant morphological characteristics and hormone endogenous levels. Free SA treatment affected length, growth rate, gravitropic response of roots and rosette size in a dose-dependent manner. This damage was due to the increase of root endogenous SA concentration that led to a reduction in auxin levels. The encapsulation process reduced the deleterious effects of free SA on root and rosette growth and in the gravitropic response. Encapsulation allowed for a controlled release of the SA, reducing the amount of hormone available and the uptake by the plant, mitigating the deleterious effects of the free SA treatment. Although both capsules are suitable as SA carrier matrices, slightly better results were found with chitosan. Encapsulation appears as an attractive technology to deliver phytohormones when crops are cultivated under adverse conditions. Moreover, it can be a good tool to perform basic experiments on phytohormone interactions. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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20 pages, 3701 KiB

Open AccessArticle

Transgenerational Tolerance to Salt and Osmotic Stresses Induced by Plant Virus Infection

by Francisco J. Hernández-Walias, Marina García, Marina Moreno, Ioannis Giannoukos, Natalia González, Eugenio Sanz-García, Khouloud Necira, Tomás Canto and Francisco Tenllado

Int. J. Mol. Sci. 2022, 23(20), 12497; https://doi.org/10.3390/ijms232012497 - 18 Oct 2022

Cited by 4 | Viewed by 1763

Abstract

Following pathogen infection, plants have developed diverse mechanisms that direct their immune systems towards more robust induction of defense responses against recurrent environmental stresses. The induced resistances could be inherited by the progenies, rendering them more tolerant to stressful events. Although within-generational induction [...] Read more.

Following pathogen infection, plants have developed diverse mechanisms that direct their immune systems towards more robust induction of defense responses against recurrent environmental stresses. The induced resistances could be inherited by the progenies, rendering them more tolerant to stressful events. Although within-generational induction of tolerance to abiotic stress is a well-documented phenomenon in virus-infected plants, the transgenerational inheritance of tolerance to abiotic stresses in their progenies has not been explored. Here, we show that infection of Nicotiana benthamiana plants by Potato virus X (PVX) and by a chimeric Plum pox virus (PPV) expressing the P25 pathogenicity protein of PVX (PPV-P25), but not by PPV, conferred tolerance to both salt and osmotic stresses to the progeny, which correlated with the level of virulence of the pathogen. This transgenerational tolerance to abiotic stresses in the progeny was partially sustained even if the plants experience a virus-free generation. Moreover, progenies from a Dicer-like3 mutant mimicked the enhanced tolerance to abiotic stress observed in progenies of PVX-infected wild-type plants. This phenotype was shown irrespective of whether Dicer-like3 parents were infected, suggesting the involvement of 24-nt small interfering RNAs in the transgenerational tolerance to abiotic stress induced by virus infection. RNAseq analysis supported the upregulation of genes related to protein folding and response to stress in the progeny of PVX-infected plants. From an environmental point of view, the significance of virus-induced transgenerational tolerance to abiotic stress could be questionable, as its induction was offset by major reproductive costs arising from a detrimental effect on seed production. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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17 pages, 3869 KiB

Open AccessArticle

Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress

by Shan Yang, Na Chu, Hongkai Zhou, Jiashuo Li, Naijie Feng, Junbo Su, Zuhu Deng, Xuefeng Shen and Dianfeng Zheng

Int. J. Mol. Sci. 2022, 23(17), 9737; https://doi.org/10.3390/ijms23179737 - 27 Aug 2022

Cited by 8 | Viewed by 2188

Abstract

Sugarcane (Saccharum spp. hybrid) is an important crop for sugar and biofuels, and often suffers from water shortages during growth. Currently, there is limited knowledge concerning the molecular mechanism involved in sugarcane response to drought stress (DS) and whether chitooligosaccharide could alleviate [...] Read more.

Sugarcane (Saccharum spp. hybrid) is an important crop for sugar and biofuels, and often suffers from water shortages during growth. Currently, there is limited knowledge concerning the molecular mechanism involved in sugarcane response to drought stress (DS) and whether chitooligosaccharide could alleviate DS. Here, we carried out a combined transcriptome and metabolome of sugarcane in three different treatment groups: control group (CG), DS group, and DS + chitooligosaccharide group (COS). A total of 12,275 (6404 up-regulated and 5871 down-regulated) differentially expressed genes (DEGs) were identified when comparing the CG and DS transcriptomes (T_CG/DS), and 2525 (1261 up-regulated and 1264 down-regulated) DEGs were identified in comparing the DS and COS transcriptomes (T_DS/COS). GO and KEGG analysis showed that DEGs associated with photosynthesis were significantly enriched and had down-regulated expression. For T_DS/COS, photosynthesis DEGs were also significantly enriched but had up-regulated expression. Together, these results indicate that DS of sugarcane has a significantly negative influence on photosynthesis, and that COS can alleviate these negative effects. In metabolome analysis, lipids, others, amino acids and derivatives and alkaloids were the main significantly different metabolites (SDMs) observed in sugarcane response to DS, and COS treatment reduced the content of these metabolites. KEGG analysis of the metabolome showed that 2-oxocarboxylic acid metabolism, ABC transporters, biosynthesis of amino acids, glucosinolate biosynthesis and valine, leucine and isoleucine biosynthesis were the top-5 KEGG enriched pathways when comparing the CG and DS metabolome (M_CG/DS). Comparing DS with COS (M_DS/COS) showed that purine metabolism and phenylalanine metabolism were enriched. Combined transcriptome and metabolome analysis revealed that pyruvate and phenylalanine metabolism were KEGG-enriched pathways for CG/DS and DS/COS, respectively. For pyruvate metabolism, 87 DEGs (47 up-regulated and 40 down-regulated) and five SDMs (1 up-regulated and 4 down-regulated) were enriched. Pyruvate was closely related with 14 DEGs (|r| > 0.99) after Pearson’s correlation analysis, and only 1 DEG (Sspon.02G0043670-1B) was positively correlated. For phenylalanine metabolism, 13 DEGs (7 up-regulated and 6 down-regulated) and 6 SDMs (1 up-regulated and 5 down-regulated) were identified. Five PAL genes were closely related with 6 SDMs through Pearson’s correlation analysis, and the novel.31257 gene had significantly up-regulated expression. Collectively, our results showed that DS has significant adverse effects on the physiology, transcriptome, and metabolome of sugarcane, particularly genes involved in photosynthesis. We further show that COS treatment can alleviate these negative effects. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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Review

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47 pages, 8328 KiB

Open AccessReview

Abiotic Stress in Crop Production

by Romana Kopecká, Michaela Kameniarová, Martin Černý, Břetislav Brzobohatý and Jan Novák

Int. J. Mol. Sci. 2023, 24(7), 6603; https://doi.org/10.3390/ijms24076603 - 01 Apr 2023

Cited by 33 | Viewed by 8272

Abstract

The vast majority of agricultural land undergoes abiotic stress that can significantly reduce agricultural yields. Understanding the mechanisms of plant defenses against stresses and putting this knowledge into practice is, therefore, an integral part of sustainable agriculture. In this review, we focus on [...] Read more.

The vast majority of agricultural land undergoes abiotic stress that can significantly reduce agricultural yields. Understanding the mechanisms of plant defenses against stresses and putting this knowledge into practice is, therefore, an integral part of sustainable agriculture. In this review, we focus on current findings in plant resistance to four cardinal abiotic stressors—drought, heat, salinity, and low temperatures. Apart from the description of the newly discovered mechanisms of signaling and resistance to abiotic stress, this review also focuses on the importance of primary and secondary metabolites, including carbohydrates, amino acids, phenolics, and phytohormones. A meta-analysis of transcriptomic studies concerning the model plant Arabidopsis demonstrates the long-observed phenomenon that abiotic stressors induce different signals and effects at the level of gene expression, but genes whose regulation is similar under most stressors can still be traced. The analysis further reveals the transcriptional modulation of Golgi-targeted proteins in response to heat stress. Our analysis also highlights several genes that are similarly regulated under all stress conditions. These genes support the central role of phytohormones in the abiotic stress response, and the importance of some of these in plant resistance has not yet been studied. Finally, this review provides information about the response to abiotic stress in major European crop plants—wheat, sugar beet, maize, potatoes, barley, sunflowers, grapes, rapeseed, tomatoes, and apples. Full article

(This article belongs to the Special Issue Drought Stress Tolerance in Plants in 2022)

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