Fermentation

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3 pages, 161 KiB

Open AccessEditorial

In Vitro Fermentation

by Mengzhi Wang

Fermentation 2023, 9(2), 86; https://doi.org/10.3390/fermentation9020086 - 19 Jan 2023

Viewed by 1234

Abstract

The rumen of ruminants, as well as the colon of monogastric animals, are inhabited by over one trillion bacteria, fungi, and protozoa, and these are emerging as critical regulators in dietary micronutrients and animal health [...] Full article

(This article belongs to the Special Issue In Vitro Fermentation)

Research

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16 pages, 591 KiB

Open AccessArticle

Comparisons of Ramie and Corn Stover Silages: Effects on Chewing Activity, Rumen Fermentation, Microbiota and Methane Emissions in Goats

by Xu Tian, Cheng Gao, Zhengping Hou, Rong Wang, Xiumin Zhang, Qiushuang Li, Zhongshan Wei, Duanqin Wu and Min Wang

Fermentation 2022, 8(9), 432; https://doi.org/10.3390/fermentation8090432 - 01 Sep 2022

Cited by 3 | Viewed by 1598

Abstract

The study aimed to investigate the nutritional value of ramie (Boehmeria nivea) silage, and its consequences for chewing activity, rumen fermentation, and enteric methane (CH₄) emissions in goats, by comparing it with corn stover (CS) silage. An in vitro [...] Read more.

The study aimed to investigate the nutritional value of ramie (Boehmeria nivea) silage, and its consequences for chewing activity, rumen fermentation, and enteric methane (CH₄) emissions in goats, by comparing it with corn stover (CS) silage. An in vitro ruminal experiment was firstly performed to investigate the substrate degradation and fermentation of CS and ramie silage. The ramie silage diet was formulated by replacing 60% of CS silage with ramie silage (dry matter (DM) basis). Eight female Xiangdong Black goats (a local breed in Southern China, 1 to 1.2 years of age) with BW of 21.0 ± 1.05 kg were used for this experiment and were randomly assigned to either one of the two dietary treatments in a cross-over design. The ramie silage had higher crude protein (CP) and ash content and lower hemicellulose content, together with decreased (p < 0.05) nutrient degradation and methane production and increased (p < 0.05) acetate molar percentage and acetate to propionate ratio through in vitro ruminal fermentation. Feeding the ramie silage diet did not alter feed intake (p > 0.05), decreased (p < 0.05) nutrient digestibility, and increased (p < 0.05) chewing activity and rumination activity, with reductions (p < 0.05) in eating activity and idle activity. Although feeding the ramie silage diet caused a greater (p < 0.05) molar percentage of acetate and lower molar percentage of propionate, it decreased the rumen-dissolved CH₄ concentration and enteric CH₄ emissions (p < 0.05). Feeding the ramie silage diet did not alter (p > 0.05) the population of bacteria, protozoa, and fungi; it increased the 16S rRNA gene copies of Ruminococcus flavefaciens (p < 0.05). Further 16SrRNA gene amplicon analysis indicated a distinct bacterial composition between the two treatments (p < 0.05). Feeding the ramie silage diet led to a lower abundance of genera Lawsonibacter, Sedimentibacter, Saccharofermentans, Sediminibacterium, and Bifidobacterium (p < 0.05). Ramie can be an alternative forage resource to stimulate chewing activity and reduce CH₄ emissions in ruminants. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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15 pages, 3217 KiB

Open AccessArticle

The Potential Use of Endophyte-Free inebrians as Sheep Feed Evaluated with In Vitro Fermentation

by Yaling Ma, Hucheng Wang, Chunjie Li and Kamran Malik

Fermentation 2022, 8(9), 419; https://doi.org/10.3390/fermentation8090419 - 24 Aug 2022

Cited by 2 | Viewed by 1576

Abstract

Endophytic fungal infection is the major reason for intoxication of animals caused by drunken horse grass. Fortunately, it has been established that seed detoxification techniques and isolation of endophytic fungi infect non-endophytic fungi populations with the same genetic background as endophyte-infected Achnatherum [...] Read more.

Endophytic fungal infection is the major reason for intoxication of animals caused by drunken horse grass. Fortunately, it has been established that seed detoxification techniques and isolation of endophytic fungi infect non-endophytic fungi populations with the same genetic background as endophyte-infected Achnatherum inebrians. Moreover, sheep can use endophyte-free Achnatherum inebriants (EF) without obvious toxicity symptoms. The present study selected EF as a representative grass, consisting of five different replacement levels, EF0, EF25, EF50, EF75, and EF100, corresponding to 0%, 25%, 50%, 75%, and 100% of the fermentation substrate, respectively. Simultaneously, in vitro fermentation and the 16S rRNA amplicon sequencing method was used to explore the effect of EF on sheep ruminal fermentation and microbial diversity. The results revealed that EF100 had the highest values for pH, acetate: propionate, the Patescibacteria, Kiritimatiellaeota, and Synergistetes phylum levels, Ruminococcaceae, Prevotellaceae, and Saccharofermentans genus levels than the other treatments (p < 0.05). In contrast, EF25 was associated with higher levels of abundance-based coverage estimator (ACE), Chaol index of the phyla Synergistetes and Bacteroidetes, and of the genus Erysipelotrichaceae, Rikenellaceae, and Prevotella as compared with other treatments (p < 0.05). EF50 resulted in the greatest values for the genus Christensenellaceae and Lachnospiraceae as compared with other treatments (p < 0.05). EF75 resulted in the greatest values for the Shannon index as compared with other treatments (p < 0.05). EF0 resulted in the greatest values for gas production (GP), ammonia nitrogen (NH₃-N), total volatile fatty acid (TVFA), acetate, propionate, butyrate, valerate, isobutyrate, isovalerate, and the phyla Firmicutes, Proteobacteria, and Spirochaetes, and the genus Succiniclasticum, Ruminobacter, Family_XIII and Treponema as compared with other treatments (p < 0.05). PICRUSt2 analysis indicated that most of the functional prediction pathways were involved in Carbohydrate metabolism and, Amino acid metabolism. Therefore, the recommended ratio of EF in sheep diet should range from 25% to 50%, and the maximum proportion should not exceed 75%. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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16 pages, 621 KiB

Open AccessArticle

Evaluation of Gas Production, Fermentation Parameters, and Nutrient Degradability in Different Proportions of Sorghum Straw and Ammoniated Wheat Straw

by Jing Wang, Zhendong Zhang, Huihui Liu, Jianfeng Xu, Ting Liu, Cailian Wang and Chen Zheng

Fermentation 2022, 8(8), 415; https://doi.org/10.3390/fermentation8080415 - 22 Aug 2022

Cited by 7 | Viewed by 1945

Abstract

The purpose of this study was to investigate the optimum proportion of sorghum straw and ammoniated wheat straw in vitro and in vivo to apply in ruminant diets. One-factor and two-factor experimental designs were used in the in vitro tests, with different ratios [...] Read more.

The purpose of this study was to investigate the optimum proportion of sorghum straw and ammoniated wheat straw in vitro and in vivo to apply in ruminant diets. One-factor and two-factor experimental designs were used in the in vitro tests, with different ratios of sorghum straw to ammoniated wheat straw (S:AWS) of 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, and 8:2 to measure the in vitro total gas production, CH₄ production, in vitro dry matter degradability (IVDMD), in vitro organic matter degradability (IVOMD) and in vitro fermentation parameters. Additionally, the nylon bag technique was used to determine the dynamic degradation of these different ratios of mixed feedstuffs for incubating in sheep rumen for 6 h, 12 h, 24 h, and 48 h. The results show that IVDMD, IVOMD, and the molar ratio of propionate were the highest when the ratio of S:AWS was 8:2 (p < 0.05) in vitro; however, this ratio released much more CH₄ (p < 0.05). In addition, the degradability of DM, OM, CP, and ash and the effective degradability of DM and CP were the highest when the ratio of S:AWS was 8:2 cultured in sheep rumen for 48 h (p < 0.05). In the in vitro and in situ nylon bag tests, IVDMD, IVOMD, rumen nutrient degradability, and effective degradability of DM and CP increased with the increase in the sorghum straw proportion. In conclusion, the higher the proportion of sorghum straw, the higher the nutrient degradability in vivo and in vitro, but also the higher the emissions of CH₄. Therefore, when the ratio of S:AWS is 8:2, ruminants can effectively utilize nutrients in feed. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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10 pages, 7480 KiB

Open AccessCommunication

Apple Pomace Modulates the Microbiota and Increases the Propionate Ratio in an In Vitro Piglet Gastrointestinal Model

by Sandrine Dufourny, Sarah Lebrun, Caroline Douny, Benjamin Dubois, Marie-Louise Scippo, José Wavreille, Pierre Rondia, Nadia Everaert and Véronique Delcenserie

Fermentation 2022, 8(8), 408; https://doi.org/10.3390/fermentation8080408 - 19 Aug 2022

Cited by 1 | Viewed by 1804

Abstract

Apple pomace (AP) contains biomolecules that induce changes in intestinal fermentation of monogastrics with positive expected health effects. The weaning of piglets can induce economic losses due to intestinal disturbances; new weaning strategies are, thus, welcome. The purpose of this study was to [...] Read more.

Apple pomace (AP) contains biomolecules that induce changes in intestinal fermentation of monogastrics with positive expected health effects. The weaning of piglets can induce economic losses due to intestinal disturbances; new weaning strategies are, thus, welcome. The purpose of this study was to test the effect of AP on fermentation products by using baby-SPIME, an in vitro multi-compartment model dedicated to piglet weaning. A comparison was done on short chain fatty acid (SCFA) ratio and the microbiota induced in bioreactors between a control culture medium vs. an AP culture medium. The results of 2 preliminary runs showed that AP medium increased the molar ratio of propionate (p = 0.021) and decreased the molar ratio of butyrate (p = 0.009). Moreover, this medium increased the cumulative relative abundance of Prevotella sp. and Akkermansia sp. in bioreactors. AP could promote an ecosystem enriched with bacteria known as next-generation probiotics (NGP)—likely influencing the energy metabolism of piglets by their fermentation metabolites. AP could be used as a dietary strategy to influence bacterial changes in the intestine by stimulating the growth of bacteria identified as NGP. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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15 pages, 2453 KiB

Open AccessArticle

Responses of Fermentation Characteristics and Microbial Communities to Vitamin B₁₂ Supplementation in In Vitro Ruminal Cultures

by Kun Wang, Zihao Liu, Chunmei Du, Benhai Xiong and Liang Yang

Fermentation 2022, 8(8), 406; https://doi.org/10.3390/fermentation8080406 - 19 Aug 2022

Cited by 2 | Viewed by 1854

Abstract

Vitamin B₁₂, an important cofactor involved in propionate formation, is synthesized exclusively by bacteria and archaebacteria. Humans need to intake vitamin B₁₂ through food, and dairy products are generally the best source of vitamin B₁₂. In the present [...] Read more.

Vitamin B₁₂, an important cofactor involved in propionate formation, is synthesized exclusively by bacteria and archaebacteria. Humans need to intake vitamin B₁₂ through food, and dairy products are generally the best source of vitamin B₁₂. In the present study, the effects of vitamin B₁₂ supplementation in diets on in vitro ruminal fermentation characteristics and microbial communities were investigated to provide a reference for increasing the vitamin B₁₂ content in milk by dietary supplementation. A completely randomized design was carried out using the in vitro rumen culture technique, and 5 vitamin B₁₂ dose levels (0, 0.5, 1.0, 2.0, and 4 mg/g of dry matter) were used. The results showed that vitamin B₁₂ supplementation in diets decreased acetate: propionate ratio and butyrate concentration. The change in the acetate: propionate ratio can be attributed to the increased relative abundances of the Proteobacteria phylum and the Negativicutes class, both of which are involved in propionate metabolism. The decrease in butyrate concentration can likely be attributed to a reduction in relative abundance of species belonging to the Clostridia class, which are known as the predominant butyrate producers in the mammalian intestine. In addition, vitamin B₁₂ supplementation in diets reduced the CH₄ production by altering the species composition of the archaeal community. In conclusion, dietary supplementation of vitamin B₁₂ resulted in rumen perturbation. In vivo studies should be conducted cautiously when evaluating the effects of vitamin B₁₂ supplementation on the synthesis and absorption of it, as well as its content in milk. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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13 pages, 2416 KiB

Open AccessArticle

Rumen Metaproteomics Highlight the Unique Contributions of Microbe-Derived Extracellular and Intracellular Proteins for In Vitro Ruminal Fermentation

by Tao Shi, Xi Guo, Yuqin Liu, Tingting Zhang, Xiangnan Wang, Zongjun Li and Yu Jiang

Fermentation 2022, 8(8), 394; https://doi.org/10.3390/fermentation8080394 - 15 Aug 2022

Cited by 1 | Viewed by 1739

Abstract

Rumen microorganisms can be used in in vitro anaerobic fermentation to encourage the sustainable exploitation of agricultural wastes. However, the understanding of active microbiota under in vitro ruminal fermentation conditions is still insufficient. To investigate how rumen microbes actively participate in the fermentation [...] Read more.

Rumen microorganisms can be used in in vitro anaerobic fermentation to encourage the sustainable exploitation of agricultural wastes. However, the understanding of active microbiota under in vitro ruminal fermentation conditions is still insufficient. To investigate how rumen microbes actively participate in the fermentation process in vitro, we resolved the metaproteome generated from ruminal fermentation broth after seven days of in vitro incubation. Herein, the sample-specific database for metaproteomic analysis was constructed according to the metagenomic data of in vitro ruminal fermentation. Based on the sample-specific database, we found in the metaproteome that Bacteroidetes and Firmicutes_A were the most active in protein expression, and over 50% of these proteins were assigned to gene categories involved in energy conversion and basic structures. On the other hand, a variety of bacteria-derived extracellular proteins, which contained carbohydrate-active enzyme domains, were found in the extracellular proteome of fermentation broth. Additionally, the bacterial intracellular/surface moonlighting proteins (ISMPs) and proteins of outer membrane vesicles were detected in the extracellular proteome, and these ISMPs were involved in maintaining microbial population size through potential adherence to substrates. The metaproteomic characterizations of microbial intracellular/extracellular proteins provide new insights into the ability of the rumen microbiome to maintain in vitro ruminal fermentation. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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14 pages, 1173 KiB

Open AccessArticle

Characterization of Lactic Acid-Producing Bacteria Isolated from Rumen: Growth, Acid and Bile Salt Tolerance, and Antimicrobial Function

by Guanghui Hu, Hui Jiang, Yujie Zong, Osmond Datsomor, Linlin Kou, Yujie An, Jingwen Zhao and Lin Miao

Fermentation 2022, 8(8), 385; https://doi.org/10.3390/fermentation8080385 - 12 Aug 2022

Cited by 3 | Viewed by 2576

Abstract

Lactic acid bacteria are some of the dominant bacteria in the rumen, and they have a high ability for lactic acid production. The present study aimed to screen and evaluate the performance of culturable rumen bacteria from Chinese Holstein dairy cows as a [...] Read more.

Lactic acid bacteria are some of the dominant bacteria in the rumen, and they have a high ability for lactic acid production. The present study aimed to screen and evaluate the performance of culturable rumen bacteria from Chinese Holstein dairy cows as a potential probiotic or inoculant for silage production, in order to isolate ruminal lactic acid bacteria and evaluate their potential as probiotics. Three strains of Enterococcus avium (E. avium, EA1-3); three strains of Streptococcus lutetiensis (S. lutetiensis, SL1-3); and six strains of Streptococcus equinus (S. equinus, SE1-6) were successfully identified from the rumen fluid using modified De Man Rogosa sharp medium supplemented with 0.325% lactic acid. E. avium, S. lutetiensis and S. equinus are clustered in the phylogenetic tree. All the 12 Gram-positive strains reached the plateau growth phase in 6–10 h, with an OD600 at about 1.8. Both gas and acid accumulation reached plateaus at about 10–12 h in all strains, and S. equinus showed the strongest capacity. The highest lactic acid accumulation was detected in S. equinus broth (up to 219.77 μmol/L). The growth of all isolates was inhibited at pH 4.0, and EA2, SL1, SL2, SL3 and SE2 were tolerant to 0.1%, 0.2% and 0.3% bile salt. In addition, the supernatants of the strains had inhibitory effects on Escherichia coli and Staphylococcus aureus. Specifically, the S. equinus strains exhibited the strongest inhibition of the pathogens. In conclusion, these 12 strains had good potential as silage inoculants or probiotics for edible animals, especially S. equinus. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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13 pages, 6693 KiB

Open AccessArticle

Mixed Fermentation of Lactiplantibacillus plantarum and Bacillus licheniformis Changed the Chemical Composition, Bacterial Community, and Rumen Degradation Rate of Tea Residue

by Xiaoyun Huang, Yinying Xu, Xinyan Wu, Yuhang Ding, Caiyun Fan, Yanfeng Xue, Zhao Zhuo and Jianbo Cheng

Fermentation 2022, 8(8), 380; https://doi.org/10.3390/fermentation8080380 - 09 Aug 2022

Cited by 4 | Viewed by 1874

Abstract

Tea residue, as a byproduct in tea processing, is highly nutritious and can be used as a good raw material for ruminant feed. This study aimed to investigate the effects of Lactiplantibacillus plantarum (L. plantarum) and Bacillus licheniformis (B. licheniformis) mixed fermentation of [...] Read more.

Tea residue, as a byproduct in tea processing, is highly nutritious and can be used as a good raw material for ruminant feed. This study aimed to investigate the effects of Lactiplantibacillus plantarum (L. plantarum) and Bacillus licheniformis (B. licheniformis) mixed fermentation of tea residue mixture (tea residue:wheat bran, 7:3) on chemical composition, bacterial community, and rumen degradation rate. Changes in chemical composition and bacterial community were evaluated after 0 (F0), 1 (F1), 3 (F3), and 5 (F5) days of fermentation. The rumen degradation rate was studied by the in situ nylon bag method. Compared with group F0, acid soluble protein in other groups increased while pH and neutral detergent fiber decreased (p < 0.05). The group F5 was the best. The diversity of bacterial communities in group F0 was significantly lower than those in the other groups (p < 0.05). The relative abundance of phylum Firmicutes and the genus Lactobacillus increased with increasing fermentation time. The rumen degradation rates of dry matter, crude protein, neutral detergent fiber, and acid detergent fiber were increased after fermentation. In conclusion, mixed fermentation of tea residue by L. plantarum and B. licheniformis can ameliorate chemical composition, reduce bacterial community diversity, and improve the rumen degradation rate. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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12 pages, 1093 KiB

Open AccessArticle

Inhibitory Effect Mediated by Deoxynivalenol on Rumen Fermentation under High-Forage Substrate

by Fan Zhang, Qichao Wu, Weikang Wang, Shanshan Guo, Wenjuan Li, Liangkang Lv, Hewei Chen, Fengliang Xiong, Yingyi Liu, Ying Chen, Shengli Li and Hongjian Yang

Fermentation 2022, 8(8), 369; https://doi.org/10.3390/fermentation8080369 - 31 Jul 2022

Cited by 5 | Viewed by 1614

Abstract

Deoxynivalenol (DON) is a type B trichothecene mycotoxin produced by Fusarium fungi. To investigate its ruminal degradability and its effect on rumen fermentation, a 2 × 5 factorial experiment was conducted in vitro with two feed substrates with different forage levels (high forage [...] Read more.

Deoxynivalenol (DON) is a type B trichothecene mycotoxin produced by Fusarium fungi. To investigate its ruminal degradability and its effect on rumen fermentation, a 2 × 5 factorial experiment was conducted in vitro with two feed substrates with different forage levels (high forage (HF), forage-to-concentrate = 4:1; low forage (LF), forage-to-concentrate = 1:4) and five DON additions per substrate (0, 5, 10, 15, and 20 mg/kg of dry matter). After 48 h incubation, the DON degradability in the HF group was higher than in the LF group (p < 0.01), and it decreased along with the increase in DON concentrations (p < 0.01), which varied from 57.18% to 29.01% at 48 h. In addition, the gas production rate, total VFA production and microbial crude protein decreased linearly against the increase in DON additions (p < 0.05). Meanwhile, the proportion of CH₄ in the fermentation gas end-products increased linearly, especially in the HF group (p < 0.01). In brief, rumen microorganisms presented 29–57% of the DON degradation ability and were particularly significant under a high-forage substrate. Along with the increasing DON addition, the toxin degradability decreased, showing a dose-dependent response. However, DON inhibited rumen fermentation and increased methane production when it exceeded 5 mg/kg of dry matter. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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15 pages, 1320 KiB

Open AccessArticle

Rumen Bacteria Abundance and Fermentation Profile during Subacute Ruminal Acidosis and Its Modulation by Aspergillus oryzae Culture in RUSITEC System

by Tongqing Guo, Tao Guo, Long Guo, Fei Li, Fadi Li and Zhiyuan Ma

Fermentation 2022, 8(7), 329; https://doi.org/10.3390/fermentation8070329 - 14 Jul 2022

Cited by 5 | Viewed by 1614

Abstract

This study aimed at characterizing changes in rumen bacteria abundance and fermentation profiles by artificial saliva (AS) pH, and at evaluating the potential modulatory role of Aspergillus oryzae culture (AOC) in a rumen simulation technique (RUSITEC) system. The treatment included high AS pH [...] Read more.

This study aimed at characterizing changes in rumen bacteria abundance and fermentation profiles by artificial saliva (AS) pH, and at evaluating the potential modulatory role of Aspergillus oryzae culture (AOC) in a rumen simulation technique (RUSITEC) system. The treatment included high AS pH (pH 6.8) or low AS pH (pH 5.5) according to the McDougall’s method, and low AS pH was sustained by changing the composition of the AS (NaHCO₃ from 9.8 to 1.96 g/L, Na₂HPO₄ from 9.3 to 1.86 g/L). In low AS pH condition, the diets contained either 0% AOC, 1.25% AOC, or 2.5% AOC. Therefore, there are four treatments: (1) high AS pH, 0% AOC (HASP); (2) low AS pH, 0% AOC (AOC0); (3) low AS pH, 1.25% AOC (AOC1); (4) low AS pH, 2.5% AOC (AOC2), respectively. The experimental diets were supplemented with 16 g basic diets with the forage to concentrate ratio of 40:60. The experiments were conducted two independent 13 days, with 9 days adaption periods and 4 days sample collection. The results showed that low AS pH decreased the degradabilites of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) (p < 0.05), which occurred due to a decreased abundance of fibrolytic Ruminococcus albus (p < 0.001). The total concentration of volatile fatty acid (VFA) and proportion of propionate were decreased in the low AS pH (p = 0.026) and tended to increase the molar proportion of butyrate (p = 0.086) and the ratio of acetate to propionate (p = 0.088). The abundances of phylum Firmicutes (p = 0.065) and Proteobacteria (p = 0.063) tended to be greater in low AS pH group than high AS pH group. Low AS pH increased the abundance of phylum Actinobacteria (p = 0.002) compared to the high AS pH and decreased the abundances of phylum Spirochaetes (p = 0.032). Compared with the high AS pH, low AS pH increased the abundances of Prevotella (p = 0.003), Pseudoscardovia (p = 0.001), Mitsuokella (p = 0.005), and Dialister (p = 0.047), and decreased the abundances of Olivibacter (p = 0.026), Ruminobacter (p = 0.025), Treponema (p = 0.037), and Sphaerochaeta (p = 0.027) at genus level. Under a severe SARA in RUSITEC, supplementation of 2.5% AOC increased OM degradability, the copy numbers of Selenomonas ruminantium and Fibrobacter succinogenes. These findings indicate that the reduction AS pH at 5.5 caused a strong shift in bacterial composition in rumen. In addition, the addition of AOC in diets increased the growth rate of certain rumen bacteria that digest fiber or utilize lactate under SARA condition in RUSITEC system. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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13 pages, 928 KiB

Open AccessArticle

Starch Properties, Nutrients Profiles, In Vitro Ruminal Fermentation and Molecular Structure of Corn Processed in Different Ways

by Chengxing Han, Yanli Guo, Xiaofang Cai and Ruixing Yang

Fermentation 2022, 8(7), 315; https://doi.org/10.3390/fermentation8070315 - 03 Jul 2022

Cited by 8 | Viewed by 2615

Abstract

Processing will improve the digestion of corn by ruminant animals. The objectives of this study were to investigate the effects of processing methods (grinding, G; steam flaking, SF; extrusion, E) on the starch properties, nutrient profiles, in vitro ruminal fermentation and molecular structure [...] Read more.

Processing will improve the digestion of corn by ruminant animals. The objectives of this study were to investigate the effects of processing methods (grinding, G; steam flaking, SF; extrusion, E) on the starch properties, nutrient profiles, in vitro ruminal fermentation and molecular structure of corn. Compared with G, SF and E increased (p < 0.05) the starch content, starch gelatinization, ruminal gas production (GP, 0.5–32 h), propionic acid, starch degradability (SD), the area and height of carbohydrate peaks, and decreased (p < 0.05) starch crystallinity, content of crude protein, neutral detergent fiber and acid detergent fiber, ruminal NH₃-N, the area and height of amide I and II, α-helix, and β-sheet. The total VFA (24 h, 48 h) tended to be increased by SF and E (p < 0.10). The carbohydrate peak area and height were positively (p < 0.05) correlated with GP (1–24 h) and SD. The protein molecular absorption intensity was negatively correlated with SD (p < 0.05). The change in starch properties, GP (1–24 h) and molecular structure caused by E was greater than SF (p < 0.05). These results indicated that the higher starch gelatinization and lower starch crystallinity of E corn, induced by the high temperature and pressure, enabled more fermentation and digestion in the artificial rumen. The carbohydrate and protein molecular structures were correlated with the nutritional characteristics of corn. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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20 pages, 1847 KiB

Open AccessArticle

The Effect of Different Lactic Acid Bacteria Inoculants on Silage Quality, Phenolic Acid Profiles, Bacterial Community and In Vitro Rumen Fermentation Characteristic of Whole Corn Silage

by Yan-Lu Wang, Wei-Kang Wang, Qi-Chao Wu, Fan Zhang, Wen-Juan Li, Zhuo-Meng Yang, Yu-Kun Bo and Hong-Jian Yang

Fermentation 2022, 8(6), 285; https://doi.org/10.3390/fermentation8060285 - 17 Jun 2022

Cited by 13 | Viewed by 2916

Abstract

Corn silage is an important source of forage, but whether or not bacterial inoculants should be applied is somewhat controversial in ruminant feeding practice. In the present study, chopped whole corn plants treated with a single inoculant of Lactobacillus buchneri (LB), Lactobacillus plantarum [...] Read more.

Corn silage is an important source of forage, but whether or not bacterial inoculants should be applied is somewhat controversial in ruminant feeding practice. In the present study, chopped whole corn plants treated with a single inoculant of Lactobacillus buchneri (LB), Lactobacillus plantarum (LP), Pediococcus pentosaceus (PP) served as either homofermentation (e.g., lactate only) or heterofermentation (e.g., lactate and acetate) controls and compared with those treated with either a mixture of the lactic acid bacteria (QA: 60% LP, 10%PP, 30% LB) or a mixture of the lactic acid bacteria (QB: 60% LP, 15% PP, 25% LB), to investigate their effects on the fermentation quality, ester-linked phenolic acids, and in vitro digestibility. After 60 day ensiling, the addition of QA exhibited the lowest pH (3.51) with greater lactic acid (LA) production. The ester-linked ferulic acid (FAest) and p-coumaric acid (pCAest) concentrations were significantly decreased during 60 days ensiling. And among all these groups, the LB and QA treated group showed a lower concentration of FAest and pCAest than other groups. After 60 days ensiling, Lactobacillus was the dominant genus in all LAB treated groups. Meanwhile, negative correlations of Bacillus, Bacteroides, Bifidobacterium, Blautia, Prevotella, Ruminococcus, and Roseburia with FAest content after 60 days ensiling occurred in the present study. Komagataeibacter was mainly found in LB and PP addition silages, and presented a significant negative effect with the level of acid detergent fiber (ADF). To explore whether the addition of LABs can improve digestibility of whole corn silage, an in vitro rumen fermentation was conducted using the 60 day ensiled whole corn silages as substrates. The QA addition group exhibited a greater 48 h and 96 h in vitro dry matter and ADF disappearance, greater 48 h gas production and less methane emissions. Even though there were the same neutral NDF levels in corn silages treated with LB and QA after 60 days ensiling, the QA treated silages with lower FAest and pCAest presented higher IVDMD after 96 h and 48 h in vitro fermentation. In brief, the addition of mixed inoculants of 60% LB,10% PP, 30% LB compared with the addition of whichever single HoLAB or HeLAB inoculants, facilitated the release of ester-linked phenolic acids (e.g., ferulic and p-coumaric acids) and remarkably, improved silage quality in terms of sharp pH decline and greater lactate production. Taken together with the improvement in rumen microbial fermentation, the results obtained in the present study provided concrete evidence for the role of mixed LAB application in corn silage preparation for ruminant feeding practices. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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15 pages, 1019 KiB

Open AccessArticle

Dynamic Variations in Rumen Fermentation Characteristics and Bacterial Community Composition during In Vitro Fermentation

by Xiao Wei, Kehui Ouyang, Tanghui Long, Zuogui Liu, Yanjiao Li and Qinghua Qiu

Fermentation 2022, 8(6), 276; https://doi.org/10.3390/fermentation8060276 - 14 Jun 2022

Cited by 12 | Viewed by 3019

Abstract

This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), fermentation at 12 h (R12, [...] Read more.

This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), fermentation at 12 h (R12, n = 10), and fermentation at 24 h (R24, n = 10). Results showed that gas production, concentrations of microbial crude protein, ammonia nitrogen, and individual volatile fatty acids (VFA), as well as total VFA and branched-chain VFA concentrations, were higher in R24 when compared with R12 (p < 0.05). However, no significant differences were observed in acetate to propionate ratio and fermentation efficiency between R12 and R24 (p > 0.05). Bacterial diversity analysis found that Shannon index and Simpson index were higher in R24 (p < 0.05), and obvious clusters were observed in rumen bacterial community between R12 and R24. Taxonomic analysis at the phylum level showed that the abundances of Proteobacteria and Fibrobacteres were higher in R12 than that in R24, and inverse results were observed in Bacteroidetes, Firmicutes, Cyanobacteria, Verrucomicrobia, Lentisphaerae, and Synergistetes abundances. Taxonomic analysis at the genus level revealed that the abundances of Rikenellaceae RC9 gut group, Succiniclasticum, Prevotellaceae UCG-003, Christensenellaceae R-7 group, Ruminococcaceae UCG-002, Veillonellaceae UCG-001, and Ruminococcaceae NK4A214 group were higher in R24, whereas higher abundances of Succinivibrionaceae UCG-002, Ruminobacter, and Fibrobacter, were found in R12. Correlation analysis revealed the negative associations between gas production and abundances of Proteobacteria, Succinivibrionaceae UCG-002, and Ruminobacter. Moreover, the abundances of Firmicutes, Rikenellaceae RC9 gut group, Christensenellaceae R-7 group, and Ruminococcaceae UCG-002 positively correlated with VFA production. These results indicate that both rumen fermentation characteristics and bacterial community composition were dynamic during in vitro fermentation, whereas the fermentation pattern, efficiency, and bacterial richness remained similar. This study provide insight into the dynamics of rumen fermentation characteristics and bacterial composition during in vitro fermentation. This study may also provide a reference for decision-making for the sampling time point when conducting an in vitro fermentation for bacterial community investigation. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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21 pages, 2673 KiB

Open AccessArticle

Assessment of Feed Value of Chicory and Lucerne for Poultry, Determination of Bioaccessibility of Their Polyphenols and Their Effects on Caecal Microbiota

by Yasir Iqbal, Eric N. Ponnampalam, Hieu Huu Le, Olivia Artaiz, Stephanie K. Muir, Joe L. Jacobs, Jeremy J. Cottrell and Frank R. Dunshea

Fermentation 2022, 8(5), 237; https://doi.org/10.3390/fermentation8050237 - 20 May 2022

Cited by 2 | Viewed by 2335

Abstract

Chicory and lucerne possess high feed value for poultry being good sources of protein and fiber. In addition, they are rich in polyphenols that help the body build an integrated antioxidant system to prevent damage from free radicals and positively modulate microbial populations [...] Read more.

Chicory and lucerne possess high feed value for poultry being good sources of protein and fiber. In addition, they are rich in polyphenols that help the body build an integrated antioxidant system to prevent damage from free radicals and positively modulate microbial populations in the gastrointestinal tract. These health-promoting effects of polyphenols depend on their bioaccessibility and absorption in the animal body. The present paper aimed to study the bioaccessibility of polyphenols from chicory and lucerne after subjecting the samples to gastric and intestinal phases of digestion in an in vitro model of chicken gut and assessment of their feed value by measuring the presence of fermentable substrates (in terms of gas production), SCFAs produced and their effects on gut microbiota population during in vitro cecal fermentation. Results revealed that the bioaccessibility of polyphenols varied with different polyphenol compounds. The highest bioaccessibility was recorded for p-hydroxybenzoic acid (90.8%) from chicory following the intestinal phase of digestion. The lowest bioaccessibility was observed for quercetin-3-rhamnoside (12.6%) from chicory after the gastric phase of digestion. From lucerne, the highest bioaccessibility was recorded for kaempferol-3-glucoside (77.5%) after the intestinal phase of digestion. Total gas production was higher for lucerne (39.9 mL/g) than chicory (28.1 mL/g). Similarly, total SCFAs production was higher after 24 h of cecal fermentation with lucerne (42.2 mmol L⁻¹) as compared to chicory (38.1 mmol L⁻¹). Results also revealed that the relative abundance of Clostridium was reduced with chicory (0.225%) and lucerne (0.176%) as compared to the control (0.550%) after 24 h of cecal fermentation. The relative abundance of Streptococcus was reduced by lucerne (4.845%) but was increased with chicory (17.267%) as compared to the control (5.204%) after 24 h of fermentation. These findings indicated that chicory and lucerne differentially affected the microbial populations during in vitro cecal fermentation. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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Review

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11 pages, 288 KiB

Open AccessReview

Advances of Rumen Functional Bacteria and the Application of Micro-Encapsulation Fermentation Technology in Ruminants: A Review

by Wenjun Wei, Yongkang Zhen, Yusu Wang, Khuram Shahzad and Mengzhi Wang

Fermentation 2022, 8(10), 564; https://doi.org/10.3390/fermentation8100564 - 20 Oct 2022

Cited by 3 | Viewed by 2508

Abstract

Rumen functional bacteria are crucial for the homeostasis of rumen fermentation and micro-ecology. Cellulolytic bacteria, amylolytic bacteria, protein- and fat-degrading bacteria, lactic acid-producing bacteria, lactic acid-consuming bacteria, methanogens, and others can all be found in the rumen flora and help the host and [...] Read more.

Rumen functional bacteria are crucial for the homeostasis of rumen fermentation and micro-ecology. Cellulolytic bacteria, amylolytic bacteria, protein- and fat-degrading bacteria, lactic acid-producing bacteria, lactic acid-consuming bacteria, methanogens, and others can all be found in the rumen flora and help the host and other microorganisms convert feed into energy. For instance, Ruminococcus flavefaciens, Ruminococcus albus, and Fibrobacter succinogenes are the three most prevalent fiber-degrading bacteria. The digestion and metabolism of various nutrients and the absorption in rumen epithelium can greatly enhance host defense mechanisms and health production in ruminants. However, directly feeding live bacteria is prone to negative environmental effects. Therefore, the micro-encapsulation of film-forming and acid-resistant wall materials can become a great means of encapsulating naked bacteria into tiny particles. It can maintain the activity of functional flora, boost the function of the intestinal barrier, and improve its capacity for colonization on the surface of the rumen and colon mucosa. Therefore, the present review evaluates the latent progress of main functional bacteria and the applied techniques of micro-encapsulation in the rumen, in order to provide more references for the development and application of rumen-functional bacteria. Full article

(This article belongs to the Special Issue In Vitro Fermentation)

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