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Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological...
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Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications: 2nd Edition

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: closed (31 March 2024) | Viewed by 4405

Special Issue Editor

Dr. Bartłomiej Zieniuk

E-Mail Website
Guest Editor
Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Warsaw, Poland
Interests: lipase-catalyzed ester synthesis; lipophilization; enzymatic (trans)esterification; whole-cell modification of phenolic compounds; microbiology; yarrowia lipolytica; lipases biosynthesis; antimicrobial and antioxidant activities of phenolic compounds; microbial enzymes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Yeasts were used for fermentation processes long before their various properties were discovered and described. Among at least 1500 recognized yeast species, in addition to the most well-known baker’s yeasts, e.g., Saccharomyces cerevisiae, other yeasts also stand out, often characterized by their unique features and capabilities.

Yarrowia lipolytica is one of the most studied “non-conventional” yeast species. The high secretory capacity and the capability of biosynthesis of many important secondary metabolites affect the growing scientific interest and biotechnological importance of this yeast. Y. lipolytica is considered to be non-pathogenic, and additionally, some commercial-scale processes with its participation have been granted GRAS (generally recognized as safe) status by the US Food and Drug Administration (FDA). The major advantages of using Y. lipolytica are its ability to grow and consume of a wide range of substrates, such as alkanes, fatty acids, fats, and oils, as well as some waste substrates, namely, crude glycerol, waste cooking oils, sewage sludge, or olive mill wastewater, with simultaneous biosynthesis of varied metabolites.

This Special Issue will compile the current state-of-the-art research on Y. lipolytica and shed light on the current research directions with the use of this yeast. Potential topics include but are not limited to the following:

- Metabolic engineering of Y. lipolytica;
- The biosynthesis of secondary metabolites, namely enzymes, e.g., lipases or proteases, as well as organic acids, sugar alcohols, flavors, and aromas;
- Whole-cell catalysis;
- Lipid biosynthesis and accumulation;
- Utilization of agri-food waste.

Dr. Bartłomiej Zieniuk
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • yarrowia lipolytica
  • secondary metabolites
  • single-cell oils (SCO)
  • lipid accumulation
  • single-cell proteins (SCP)
  • enzymes biosynthesis
  • lipases production
  • metabolic engineering
  • organic acids synthesis
  • agri-food waste upgradation
  • whole-cell catalysis
  • biosynthesis of metal nanoparticles
  • bioreactor processes

Related Special Issue

Published Papers (3 papers)

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Research

16 pages, 2577 KiB  
Article
Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered Yarrowia lipolytica Strain Using Co-Substrate Strategy
by Masoud Tourang, Xiaochao Xiong, Sara Sarkhosh and Shulin Chen
Fermentation 2023, 9(12), 1003; https://doi.org/10.3390/fermentation9121003 - 29 Nov 2023
Viewed by 1433
Abstract
High production cost is one of the major factors that limit the market growth of polyhydroxyalkanoates (PHAs) as a biopolymer. Improving PHA synthesis performance and utilizing low-grade feedstocks are two logical strategies for reducing costs. As an oleaginous yeast, Y. lipolytica has a [...] Read more.
High production cost is one of the major factors that limit the market growth of polyhydroxyalkanoates (PHAs) as a biopolymer. Improving PHA synthesis performance and utilizing low-grade feedstocks are two logical strategies for reducing costs. As an oleaginous yeast, Y. lipolytica has a high carbon flux through acetyl-CoA (the main PHB precursor), which makes it a desired cell factory for PHB biosynthesis. In the current study, two different metabolic pathways (NBC and ABC) were introduced into Y. lipolytica PO1f for synthesizing PHB. Compared to the ABC pathway, the NBC pathway, which includes NphT7 to redirect the lipogenesis pathway and catalyze acetoacetyl-CoA synthesis in a more energy-favored reaction, led to PHB accumulation of up to 11% of cell dry weight (CDW), whereas the ABC pathway resulted in non-detectable accumulations of PHB. Further modifications of the strain with the NBC pathway through peroxisomal compartmentalization and gene dose overexpression reached 41% PHB of CDW and a growth rate of 0.227 h−1. A low growth rate was observed with acetate as the sole source of carbon and energy or glucose as the sole substrate at high concentrations. Using a co-substrate strategy helped overcoming the inhibitory and toxic effects of both substrates. Cultivating the engineered strain in the optimal co-substrate condition predicted by response surface methodology (RSM) led to 83.4 g/L of biomass concentration and 31.7 g/L of PHB. These results offer insight into a more cost-effective production of PHB with engineered Y. lipolytica. Full article
(This article belongs to the Special Issue Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications: 2nd Edition)
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14 pages, 1818 KiB  
Article
Valorization of Macauba (Acromia aculeata) for Integrated Production of Lipase by Yarrowia lipolytica and Biodiesel Esters
by Filipe Smith Buarque, Marcelle A. Farias, Júlio Cesar Soares Sales, Adriano Carniel, Bernardo Dias Ribeiro, Verônica Regina de Oliveira Lopes, Aline Machado Castro and Maria Alice Zarur Coelho
Fermentation 2023, 9(12), 992; https://doi.org/10.3390/fermentation9120992 - 21 Nov 2023
Viewed by 1028
Abstract
Enzymatic biodiesel production is a potential eco-friendly alternative to the conventional chemical route which requires extensive study to reduce the costs associated with the application of commercial enzymes. Thus, this study aimed to develop a bioprocess using residues from macauba (Acrocomia aculeata [...] Read more.
Enzymatic biodiesel production is a potential eco-friendly alternative to the conventional chemical route which requires extensive study to reduce the costs associated with the application of commercial enzymes. Thus, this study aimed to develop a bioprocess using residues from macauba (Acrocomia aculeata) as raw material for lipase production in solid-state fermentation (SSF) by Yarrowia lipolytica. Then, the product obtained was used as a biocatalyst for the conversion (hydrolysis/esterification) of macauba acidic oil to biodiesel esters. Firstly, different SSF parameters (inoculum concentration, initial moisture content, and carbon and nitrogen levels) were investigated in a factorial design approach, using the cake from macauba fruit. Afterwards, moisture and urea concentration were shown to be statistically significant variables for lipase production. Lipase productnivities were 12.6 ± 0.6 U g−1 h−1 (at 24 h) for macauba fruit cake and 11.6 ± 1 U g−1 h−1 (at 20 h) for macauba pulp and peel cake. The solid enzymatic preparation (biocatalyst) showed optimized values at pH 6–7 at 37 °C, remaining stable (>70% retention) for 90 days at room temperature. Finally, enzymatic hydrolysis of the acidic oil from macauba reached 96% conversion (72 h) to fatty acids, and esterification of fatty acids reached 72% (biodiesel yield of 67%). The bioprocess described is a promising alternative for an integral and self-sufficient valorization of the macauba products. Full article
(This article belongs to the Special Issue Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications: 2nd Edition)
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16 pages, 2119 KiB  
Article
Solid State and Semi-Solid Fermentations of Olive and Sunflower Cakes with Yarrowia lipolytica: Impact of Biological and Physical Pretreatments
by Ana Rita Costa, Helena Fernandes, José Manuel Salgado and Isabel Belo
Fermentation 2023, 9(8), 734; https://doi.org/10.3390/fermentation9080734 - 06 Aug 2023
Cited by 3 | Viewed by 1498
Abstract
Lignocellulosic biomass is a promising feedstock for added value compound production in biotechnological processes such as solid-state fermentation (SSF). Although these solid materials can be directly used as substrates in fermentations in a solid state, a pretreatment is often required, especially if the [...] Read more.
Lignocellulosic biomass is a promising feedstock for added value compound production in biotechnological processes such as solid-state fermentation (SSF). Although these solid materials can be directly used as substrates in fermentations in a solid state, a pretreatment is often required, especially if the microorganism selected is unable to produce lignocellulosic enzymes. In the present work, several pretreatment strategies were applied to a 50% (w/w) mixture of olive and sunflower cakes before SSF for lipase production by the oleaginous yeast Yarrowia lipolytica W29. Co-culture strategies with Y. lipolytica and Aspergillus niger did not improve lipase production by the oleaginous yeast. Biological pretreatment with a fungal enzymatic extract led to a significant increase in sugar availability in the substrate mixture after a short incubation period, improving yeast growth. Microwave and ultrasound were the physical pretreatments selected and microwave irradiation proved to be the best method, resulting in 44% and 17% increases in yeast growth and lipase production, respectively, compared to the untreated mixture. An improvement in lipase activity was also observed after ultrasonic treatment in semi-solid fermentations, leading to a 2-fold increase in this enzyme activity compared to the control. The utilization of pretreatments before SSF with Y. lipolytica can increase sugars availability and result in structural changes in the solid substrate, which can improve the bioprocesses’ productivity. Full article
(This article belongs to the Special Issue Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications: 2nd Edition)
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