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Fermentation
Special Issues
Green Synthesis of Antimicrobials and Its Applications
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Green Synthesis of Antimicrobials and Its Applications

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Industrial Fermentation".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 686

Special Issue Editors

Dr. Jaslyn Jie Lin Lee

E-Mail Website
Guest Editor
N&E Innovations Pte Ltd. 9 Chin Bee Drive #03-01, Singapore 619860, Singapore
Interests: food waste valorization for the production of sustainable food packaging; novel food polymers; food ingredients; antimicrobial compounds
Dr. Kong Fei Chai

E-Mail Website
Guest Editor
Food Science and Technology Programme, School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore 637459, Singapore
Interests: fermentation technology; food waste valorization; fats and oils and bioactive peptides
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The excessive use of antibiotics has led to the development of antimicrobial resistance (AMR). The United Nations estimates that the number of deaths caused by AMR could reach up to 10 million per year by 2050. In recognition of this pressing global crisis, it is with great privilege that we introduce a Special Issue entitled “Advances in Green Synthesis of Antimicrobials and Their Applications,” with a particular emphasis on the pivotal role played by fermentation.

In alignment with our steadfast commitment to addressing this critical public health challenge, this Special Issue serves as a platform for unveiling emerging trends and breakthroughs within the domain of innovative natural antimicrobials. At its core, this compilation advocates the integration of green chemistry principles, in conjunction with the adoption of sustainable feedstock and methodologies, for the synthesis of these essential antimicrobial agents. Amidst this pursuit, it is imperative to underscore the inseparable connection between the science of antimicrobial potency and the principles of environmental stewardship.

Our endeavor bears substantial significance due to our emphasis on the catalytic role of fermentation in the synthesis of these remarkable antimicrobials. As an exemplar of sustainable production, fermentation resonates harmoniously with the principles of green synthesis. Thus, we extend an invitation to scholars, researchers, and practitioners to share their profound insights into the synergy of fermentation techniques with antimicrobial synthesis.

This Special Issue aims to focus on new trends and developments in novel natural antimicrobials synthesized using green chemistry or other sustainable feedstock or methods. In addition, applications demonstrating the use of novel antimicrobials or in vitro and in vivo toxicity studies are in line with this Issue. Research papers or reviews are also welcome.

Dr. Jaslyn Jie Lin Lee
Dr. Kong Fei Chai
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial
  • green synthesis
  • natural
  • sustainable
  • applications

Published Papers (1 paper)

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Research

15 pages, 2747 KiB  
Article
Production of Polyclonal Antibodies and Development of Competitive ELISA for Quantification of the Lantibiotic Paenibacillin
by Ahmed G. Abdelhamid, Macdonald Wick and Ahmed E. Yousef
Fermentation 2024, 10(5), 232; https://doi.org/10.3390/fermentation10050232 - 26 Apr 2024
Viewed by 248
Abstract
The discovery and biotechnological application of new antimicrobial peptides are impeded by a lack of sensitive methods for peptide quantification. Paenibacillin is an emerging antimicrobial lantibiotic that was discovered in Paenibacillus polymyxa OSY-DF ATCC PTA-7852, isolated from the fermented vegetable Kimchee. This lantibiotic [...] Read more.
The discovery and biotechnological application of new antimicrobial peptides are impeded by a lack of sensitive methods for peptide quantification. Paenibacillin is an emerging antimicrobial lantibiotic that was discovered in Paenibacillus polymyxa OSY-DF ATCC PTA-7852, isolated from the fermented vegetable Kimchee. This lantibiotic has potency against many foodborne pathogenic and spoilage bacteria. To advance the research and application of paenibacillin, a rapid, specific, and sensitive detection and quantification immunoassay was developed. After anti-paenibacillin polyclonal antibodies (pAbs) were generated and purified, a competitive enzyme-linked immunosorbent assay (cELISA) was developed and optimized for paenibacillin quantification. The dynamic range of the cELISA was determined by using a three-parameter nonlinear regression model, achieving a correlation (R2) value of 0.95. The cELISA displayed high sensitivity, with the ability to detect paenibacillin at levels as low as 15.6 ng/mL, which is significantly lower than the limit of detection of the conventional antimicrobial assay (20 µg/mL paenibacillin). The cELISA successfully differentiated paenibacillin concentrations in cell-free crude supernatants of P. polymyxa wild type and its mutant strain when grown at 30 °C and 37 °C; higher paenibacillin levels were found in the mutant (0.248–0.276 µg/mL) than in the wild type (0.122–0.212 µg/mL) culture. These findings were validated by the transcriptional analysis of 11 paenibacillin biosynthetic genes, which were significantly upregulated (≥2-fold increase) in the mutant compared with the wild strain. Additionally, the cELISA exhibited high sensitivity by recovery of paenibacillin titers spiked at 2.5 and 10 µg/mL in de Man, Rogosa, and Sharpe (MRS) broth and diluted skim milk. These results suggest that the anti-paenibacillin pAbs and the developed cELISA could be valuable in quantifying paenibacillin in complex matrices and in aiding the discovery of paenibacillin-producing natural microbiota. Full article
(This article belongs to the Special Issue Green Synthesis of Antimicrobials and Its Applications)
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