Ecologies

Soil samples taken during archaeological investigations of a historical tannery area in the eastern suburb of the medieval city of Jena have been investigated by 16S r-RNA gene profiling. The analyses supplied a large spectrum of interesting bacteria, among them Patescibacteria, Methylomirabilota, Asgardarchaeota, Zixibacteria, Sideroxydans and Sulfurifustis. Samples taken from soil inside the residues of large vats show large differences in comparison to the environmental soil. The PCAs for different abundance classes clearly reflect the higher similarity between the bacterial communities of the outside-vat soils in comparison with three of the inside-vat soil communities. Two of the in-side vat soils are distinguishable from the other samples by separate use of each abundance class, but classes of lower abundance are better applicable than the highly abundant bacteria for distinguishing the sampling sites by PCA, in general. This effect could be interpreted by the assumption that less abundant types in the 16S r-RNA data tend to be more related to an earlier state of soil development than the more abundant and might be, therefore, better suited for conclusions on the state of the soils in an earlier local situation. In addition, the analyses allowed identification of specific features of each single sampling site. In one site specifically, DNA hints of animal residue-related bacteria were found. Obviously, the special situation in the in-site vat soils contributes to the diversity of the place, and enhances its Beta-diversity. Very high abundancies of several ammonia-metabolizing and of sulphur compound-oxidizing genera in the metagenomics data can be interpreted as an echo of the former tannery activities using urine and processing keratin-rich animal materials. In summary, it can be concluded that the 16S r-RNA analysis of such archaeological places can supply a lot of data related to ancient human impacts, representing a kind of “ecological memory of soil”. Full article

This study investigated the content of ¹³⁷Cs (a long-lived radioactive isotope of caesium) in various parts of Pinus sylvestris L. (Scotch pine) and Dicranum polysetum Sw. (rugose fork-moss) at three different sites within the exclusion zone of the Chornobyl nuclear power plant over two years. The Leliv site is located within the 10 km zone, while the Paryshiv and Dytiatky sampling sites are within the 30 km zone. Samples of different P. sylvestris organs were collected, including 1- and 2-year-old branches and needles and wood and outer bark, and the entire D. polysetum. Sampling was conducted every two weeks throughout the year during 2014 and 2015. The specific activity levels of ¹³⁷Cs in the samples were measured using gamma spectrometry with a CANBERRA gamma spectrometer unit and a coaxial high-purity HPGe semiconductor detector. The study found that at the Leliv and Paryshiv sites, the highest content of ¹³⁷Cs in living organs of P. sylvestris was found in the wood. At the Dytiatky site, the needles and branches of the first and second years had anomalously high concentrations of radiocaesium (¹³⁷Cs). This could be due to a thin layer of forest litter (1.5 cm) at that site. The study also found significant changes in the specific activity levels of ¹³⁷Cs in living pine organs throughout the year. The highest concentration was observed in pine branches and needles in summer, and the maximum values in wood were observed in winter. The study suggests that a constant circulation of ¹³⁷Cs in the soil–plant system can cause seasonal changes in the content of ¹³⁷Cs in living pine organs. Symbiotic mycorrhizal fungi can play an important role in the circulation of radiocaesium in forest ecosystems. The outer bark of P. sylvestris did not show any seasonal changes in the content of ¹³⁷Cs. It may not be involved in radiocaesium redistribution inside the plant but can serve as a long-term source of this radionuclide entering the forest litter. The study found no seasonal changes in the accumulation of ¹³⁷Cs by D. polysetum, which might be due to the physiological characteristics of this plant species. Based on the analysis of the conducted studies, the recommendation is to consider the seasonal changes in the content of ¹³⁷Cs during monitoring activities and when using Scots pine in areas potentially contaminated with this radionuclide. Full article

The northern coast of the Iberian Peninsula is an important spawning and nursery area for several marine fish species, some of which are economically exploited by fisheries and under management plans. Larval stages of fish are highly sensitive to environmental change and anthropogenic pressures, and Marine Protected Areas (MPA) can help mitigate the impacts on fish populations. This study investigated the environmental drivers of the temporal and spatial patterns of the larval fish assemblages inhabiting a small coastal MPA along the NW Portuguese Iberian Coast. Seasonal surveys were conducted over two years at nine sampling stations distributed throughout the MPA to collect larval fish samples and water parameters. Results showed that a total of 39 different fish taxa were identified. In terms of abundance, reef-associated species, such as Parablennius gattorugine (54.6%), and marine species that use estuaries as nursery areas, such as Ammodytes tobianus (15.7%) and Clupeidae n.i. (8.8%) dominated the larval fish assemblages. The larval fish assemblages were characterized by a strong temporal pattern that, according to CCA analyses, was related to the temporal variability of water temperature, pH, chlorophyll α, TPM, and also the river flow of an adjacent river. This study showed that 47% of the fish larvae belonged to commercially exploited species, highlighting the importance of this MPA as a nursery area for the early life stages of the fish population. Overall, these new findings emphasize the role of MPAs in ensuring the connectivity of fish species between marine-estuarine habitats and enhancing the conservation of fish populations. Full article

Currently, there is a world coffee production crisis which has been attributed, among other factors, to the COVID-19 pandemic that affected the development of productive agricultural activities. In this scenario, Mexico is in a declining situation by showing a reduction in coffee production areas in recent years. Therefore, it is necessary to promote actions that contribute to the recovery of the resource, particularly in the states with agricultural potential. In the present work, the potentially suitable areas for coffee cultivation are identified through the application of tools that allow for characterizing the biophysical conditions that define the current spatial distribution and, from the analysis of these characteristics, generate a Potential Distribution Model (PDM) of the suitable zones for coffee production. The methodology was developed through the application of the Maximum Entropy (MaxEnt) algorithm, starting with the collection and preparation of coffee presence records, followed by a correlation analysis and identification of significant variables, the subsequent execution of the model in various configurations to observe the contribution of each variable through a jackknife test, and finally validation of the model with a random sample selection of 30%, to achieve an AUC of 0.98 and TSS of 0.96. The present model was able to identify and quantify the environmentally suitable zones for coffee production, highlighting the regions with ideal potential for the specie. These results are intended to serve as a basis for the generation of planning strategies aimed at managing, improving, and increasing coffee production areas, as well as being used to establish biological corridors to promote biodiversity, conservation, and alternative economic activities such as tourism and furthermore for future work on the analysis of production scenarios and impacts of climate change. It is concluded that 30% of Nayarit’s territory has ideal conditions for coffee cultivation, especially the region delimited by the municipalities of Tepic and Xalisco, the eastern zone of Compostela, and the southwest of San Blas, which should be considered as a Priority Conservation Area (APC) for coffee cultivation in the state. Full article

The problem of assessing the impact of pollution in protected areas prompted us to apply a combined method of bioindication and spatial mapping of phytoplankton data from six lakes in the Burabay National Nature Park in Northern Kazakhstan. The issue of monitoring planning was quite acute for this landscape-homogeneous area among the Kulunda steppes. Phytoplankton in each of the six lakes was collected in the summer season of 2019 at a total of 54 sampling stations. In total, 139 species of algae and cyanobacteria from seven taxonomic phyla were found in the phytoplankton of the Burabay Park during the study period. Three phyla were the richest in species: diatoms, green algae, and cyanobacteria. Based on species richness, abundance, and biomass, as well as bioindicators and calculated indices of organic pollution and toxic effects, the current ecological state of the lake was assessed as being under the influence of pollution, of the mesotrophic type and with a high capacity for self-purification. Statistical mapping, calculated by the correlation of the species composition and categories of indicators, revealed the zones of anthropogenic impact located on the shores of the lake, and the water of the lakes as weakly alkaline, quality classes 2–3. An increase in the number of cyanobacteria in coastal communities was revealed, which may be associated with an increase in the biogenic load on the lake ecosystems. The results of the analysis and mapping of indicators revealed that two major factors regulated phytoplankton: salinity and organic pollution. The sources of organic pollution are mostly associated with the intake of substances from the coastal zone, where resorts, roads, and settlements are located. Full article

Stem diameter growth in living trees refers to the invariably increase in dimension over a given period. However, reversible stem diameter decrease could occur, related to water movement in the vessels within the wood (on a daily basis) and to tree water deficit and depletion of stored water (on a seasonal basis). Recently, the perennial decrement in stem diameter size has been related to a tree decline pathway, and eventually resulting in tree death. In this study, we analysed stem diameter intra-annual growth dynamics of holm oak (Quercus rotundifolia Lam.) trees for two full growing seasons in distinct woodlands, Laborela and Aldeia dos Fernandes, in southern Portugal (Ourique district). Our focus was to assess stem diameter growth patterns and trends in holm oak trees in endangered woodlands with high tree mortality and to question if perennial decrement. Holm oaks in Laborela were much more sensitive in their stem diameter variations than in Aldeia dos Fernandes and, on average, their stem diameter decreased continuously by 1.0 mm along the 2-year study period, with a slightly higher annual decrease in the first study year (0.6 mm.yr⁻¹). In addition, on average, trees had a higher decrease in stem sectional area of 5.8 cm² in Laborela against a decrease of 3.7 cm² in Aldeia dos Fernandes, where the stem diameter of holm oaks only decreased in the first study year (0.1 mm.yr⁻¹). In each study area, the repeated- measures ANOVA showed that tree size effect influenced the stem diameter variations in contrast to tree crown defoliation. Trees were, on average, relatively smaller in Laborela, with DBH = 35.5 cm against DBH = 40.6 cm in Aldeia dos Fernandes, and were highly sensitive in their stem diameter decrement along two consecutives full growing seasons. This is the first exploratory study on assessing the stem diameter fluctuations on holm oaks to address a decline pathway. Our results revealed that holm oaks can survive during two consecutive growing seasons, with a continuous decrease in their stem diameters, which might indicate one tree’s decline pathway. Full article

In this study, aquatic macroinvertebrates were used as bioindicators to determine the ecological conditions of the Lapa River, located between the municipalities of Cayey and Salinas, Puerto Rico. The water quality of the river was evaluated through the calculation of the Puerto Rico Biological Monitoring Working Party (BMWP-PR), as well as its degree of contamination using the Puerto Rico Family Biotic Index (FBI-PR). Bimonthly sampling was conducted across four sampling sites for a period of 12 months. The sampling sites were positioned upstream and downstream within the nature reserve, and outside and downstream its borders. The BMWP-PR results showed that the site upstream-inside the nature reserve had good water quality, and the site downstream-inside the nature reserve had regular water quality, showing some areas with eutrophication. The water quality outside-downstream from the nature reserve was poor. The FBI-PR results showed that there was mild organic contamination inside the nature reserve, while there was substantial organic contamination in the site that was outside-downstream from the nature reserve. We concluded that the section of the river located within the nature reserve had better ecological conditions than the stretch of the river located outside-downstream of the nature reserve, because it is located within a protected area that has barely been impacted by human activity. Full article

Agricultural expansion has had a detrimental effect on tropical forests and the animal communities that depend on them. Agroforestry systems, however, with their more complex tree and plant communities, have been shown to be important habitats for a range of globally threatened species, including nocturnal animals. Here, we present novel data on the encounter rates of seven species of nocturnal mammals in relation to agroforestry systems within four national parks and associated plantations in Bangladesh to examine if encounter rates were influenced by the human population density, presence of plantations, and human access as represented by a Human Influence Index of anthropogenic disturbance. We walked 70.3 km of transects with only semi-natural forest, 26.9 km of transects with semi-natural forest and gardens, and 21.7 km of transects with semi-natural forest and monocultures over 55 nights from 2017–2019. Of the seven species of nocturnal mammals we detected, all were present in Satachari National Park, whereas six occurred in Lawachara National Park, Rajkandi Forest Range, and Rema-Kalenga Wildlife Sanctuary. Within these national parks, three species (Bengal slow loris, large Indian civet, particolored flying squirrel) were more frequently recorded in areas with human disturbance, especially agroforestry plantations. With declining forest cover in Bangladesh, we highlight here the potential of agroforestry systems as emerging important habitats for these species. We encourage long-term studies of these lesser-studied taxa to understand fully the capacity of agroforestry systems in order to support their long-term conservation. Full article

Hummingbirds are charismatic fauna that provide important pollination services, including in the continental US, where 15 species regularly breed. Compared to other birds in North America, hummingbirds (family Trochilidae) have a unique exposure route to pesticides because they forage on nectar. Therefore, hummingbirds may be exposed to systemic pesticides borne in nectar. They also may be particularly vulnerable to pesticide exposure due to their small size and extreme metabolic demands. We review relevant factors including hummingbird life history, nectar residue uptake, and avian bioenergetic considerations with the goal of clearly identifying and articulating the specific modeling challenges that must be overcome to develop and/or adapt existing modeling approaches. To help evaluate these factors, we developed a dataset for ruby-throated hummingbirds (Archilochus colubris) and other avian species potentially exposed to pesticides. We used the systemic neonicotinoid pesticide imidacloprid as an illustration and compared results to five other common current use pesticides. We use the structure of Pop-GUIDE to provide a conceptual modeling framework for implementation of MCnest and to compile parameter values and relevant algorithms to predict the effects of pesticide exposure on avian pollinators. Conservative screening assessments suggest the potential for adverse effects from imidacloprid, as do more refined assessments, though many important limitations and uncertainties remain. Our review found many areas in which current USEPA avian models must be improved in order to conduct a full higher-tier risk assessment for avian pollinators exposed to neonicotinoid insecticides, including addition of models suitable for soil and seed treatments within the MCnest environment, ability to include empirical residue data in both nectar and invertebrates rather than relying on existing nomograms, expansion of MCnest to a full annual cycle, and increased representation of spatial heterogeneity. Although this work focuses on hummingbirds, the methods and recommendations may apply more widely to other vertebrate pollinators. Full article

Lichens are symbiotic partnerships between a filamentous fungus and a photosymbiotic “alga”. Studies show that lichens harbor endothallic fungi, but that some taxa have been difficult to isolate from the main filamentous thallus-forming fungus and other faster growing lichenicolous/endothallic fungi. Therefore, we aimed to develop and evaluate liquid yeast-enrichment strategies to (1) isolate lichen-associated yeasts in pure culture, and (2) determine the taxonomic placement and breadth of the diversity of culturable yeasts. Eighty-two lichen samples were collected and washed with distilled water, and healthy thalli were ground up and added to seven different yeast-enrichment broths. Yeast colonies were isolated in pure culture and identified using molecular techniques. Initial isolates were identified using BLASTn analysis, and a taxonomic refinement was completed using PhyML analysis. In total, 215 isolates were obtained. The most prevalently isolated ascomycetous yeasts were within the Dothideomycetes (Aureobasidium, Plowrightia, and Dothiora), while the most frequently isolated basidiomycetous yeasts belonged to the genera Curvibasidium, Sporobolomyces, and Tremella. The generic placements could not be determined for 17 isolates, and in total 25 novel species were recovered. The results of this research indicate that (1) lichen-associated yeasts are diverse, (2) employing liquid enrichment strategies is effective for isolating many of these, and (3) lichen thalli represent a valuable untapped reservoir of diverse and novel yeast species. Full article

Nanoparticles are man-made materials defined as materials smaller than 100 nm in at least one dimension. Titanium oxide nanoparticles are of great interest because of their extensive use in self-care products. There is a lack of nanotoxicological studies of TiO₂ NPs in benthic organisms to have evidence about the effects of these pollutants in freshwater ecosystems. Atya lanipes is a scraper/filter that can provide a good nanotoxicological model. This study aims to determine how the TiO₂ NPs can develop a toxic effect in the larvae of the Atya lanipes shrimp and to document lethal and sublethal effects after acute exposures to TiO₂ NP suspensions of: 0.0, 1.0, 10.0, 50.0, 100.0, and 150.0 mg/L. The results show that early exposure to TiO₂ NPs in Atya lanipes creates an increase in mortality at 48 and 72 h exposures, hypoactivity in movements, and morphological changes, such as less pigmentation and the presence of edema in exposed larvae. In conclusion, TiO₂ NPs are toxic contaminants in the larval stage of the Atya lanipes. It is necessary to regulate these nanoparticles for purposes of the conservation of aquatic biodiversity, especially for freshwater shrimp larvae and likely many other larvae of filter-feeding species. Full article

Impact of pollination on the agri-food sector is of paramount importance. Pollinators contribute to the maintenance of ecosystems, the reproduction and survival of many plants, and their presence usually leads to increased yield and quality of agricultural products. Breeding and selecting for plant traits for enhancing pollinator visits could therefore lead to more resilient farming systems. In stating the advantages of enhancing pollinators in agricultural systems, this study was designed aiming to assess six cowpea accessions for their flower traits and their effect on insect-pollinators. Pollinators species abundance and foraging activity was recorded and their impact on yield was investigated. Twenty-five of the twenty-seven flower traits studied differed statistically significantly among cowpea accessions. The main pollinators recorded belonged to the genus Xylocopa (Latreille, 1802). Seed and fresh pod yield was not affected by pollinators. The floral traits related to pollinators abundance and foraging activity were flower color, inflorescence position and the hours that the flowers per plant remained open during the day. However, they were not related linearly to pollinators abundance and foraging activity; therefore, they did not constitute safe traits for selection aiming to increase pollinators visitation. The findings suggested that other traits, such as pollen and nectar reward, probably perform a more important role in attracting pollinators compared to flower traits. Full article

Hot springs are known as highly adverse extreme environments where thermophilic and hyperthermophilic microorganisms can survive. We describe taxonomic diversity of several microbial biofilms collected along water temperature gradient in hot streams in the aquatic system of the Stolbovskie hot springs on Kunashir Island, Kurils, Russia. The taxonomic composition of the studied microbial communities was assessed by the 16S rRNA gene metabarcoding for bacteria and archaea, and by the 18S rRNA gene metabarcoding for protists. Richness and diversity of bacteria in the geothermal microbial communities decreased with the increase of temperature, while for archaea, the tendency was the opposite. Ciliophora was the most represented taxon of protists. The biofilms of various kinds that we found in a very local area of the geothermal system were different from each other by taxonomic composition, and the level of their taxonomic diversity was significantly influenced by water temperature. Full article

The objective of the manuscript was to study the effects of dead wood on macroinvertebrates (MI) assemblages occupying different habitat patches. Three streams were studied, with control and wooded sections in each stream from which MI were sampled for all substrate types (wood, fine detritus, gravel, sand). This contribution deals with three German streams Ihle, Nieplitz, and Verlorenwasser, which belong to the stream type 14 (sand-dominated lowland stream) and are located in the landscape unit Fläming. This type of stream, where large wood could be most influential, has been relatively understudied. This study focused on quantifying substrate distribution and macroinvertebrate taxonomy and performing statistical analysis of MI densities with regard to substrate type. The area-specific invertebrate assemblages were differentiated for MI density, dry matter density and taxonomic richness of invertebrates. In addition to macroinvertebrates, a special focus was on aquatic and semi-aquatic insects. There were significant differences between the control and wooden sections. The macroinvertebrate assemblages (individual density, dry matter density and species richness) of the detritus of the wood sections only in the Verlorenwasser was significantly and positively affected (two or three times) by the deadwood. Detritus in the wood sections of the Nieplitz and Verlorenwasser contained more sensitive species like EPT than detritus from the control sections. On the other hand, no significant difference in MI populations were determined for the sand of the wood sections compared to the sand of the control sections. In all water bodies, the wood substrate promoted all investigated indicators of macrozoobenthos as compared to sand of the wood sections. Detritus significantly promoted macrozoobenthos colonization in the wood sections as compared to sand of the Nieplitz and the Verlorenwasser. The MI density and dry matter density in the Ihle was also significantly promoted by detritus, although not significantly with regard species richness. Two significant correlations were also identified. The more sand that was present in the Ihle and the Verlorenwasser, the more the macroinvertebrate assemblages were inhibited. Additionally, the larger the area of detritus in the Ihle and the area of wood in the Nieplitz, the more MI populations were encouraged. The influence of the deadwood position on MI was also investigated, but there was no significant difference. Incline bridges, flow guides and parallel wood position types were settled in similar quality and quantity. Full article

Rhizosphere soil ecosystems are represented by the diversity of different soil aggregate-size classes, such as large macroaggregates, small macroaggregates, mesoaggregates, and microaggregates. Though these aggregate-size classes represent distinct biological, chemical, and physical properties, little is known about their dynamics and relationships with belowground and aboveground plant traits. In this study, we examined the relationships of various soil aggregate-size classes and their organic carbon contents with many aboveground and belowground soybean plant traits. Our study revealed several novel and interesting relationships between soil structural properties and plant traits. Notably, small macroaggregates represented a major portion of the rhizosphere soil ecosystem of soybean plants while organic carbon contents decreased with decreasing size of soil aggregates. Only microaggregates showed a significant relationship with root architectural traits, such as length and surface area. Among all soil aggregate size classes, the abundance of small macroaggregates and the organic carbon contents of microaggregates were better correlated with plant traits. In general, organic carbon contents of different soil aggregate-size classes showed positive correlations with leaf trichome density (defense traits) and major macronutrients, such as root P, K, and S contents; while there were mostly negative correlations with some micronutrient (Ca, Mn, Zn, Cu, B, and Mg) contents of roots and shoots. However, the abundance of small macroaggregates mostly positively correlated with the mineral contents of plant roots and shoots. Collectively, the positive and negative correlations of organic carbon contents of different soil aggregate-size classes with trichomes (defense) and physiological traits (micro-mineral contents) suggest their significance in plant nutrition and defense. Though our results suggest the relationships of soil aggregate properties with aboveground and belowground traits, further research is needed to discern the role of soil structural traits in mediating plant growth, development, defense, and physiology. Full article

Assessing a taxon’s response to change in environmental variables is fundamental knowledge to understanding trends in species diversity, abundance, and distribution patterns. This is particularly needed on Borneo, where knowledge on Odonata populations in different habitats is poor. To address this gap, we present the first study investigating the relationship between morphology and species distribution of Odonata communities in a heath (kerangas)-dominated mixed-mosaic-lowland forest in southern Borneo. We sampled 250-m line transects in three habitat types: mixed peatcswamp, kerangas, and low-pole peatcswamp, with weekly surveys from December 2019 to February 2020. A total of 309 individuals were detected from 25 species. Anisoptera and Zygoptera diversity was the highest in mixed peatcswamp and lowest in low pole, while abundance was the highest in low pole and lowest in kerangas; with kerangas notably harboring a very small sample size. Odonata community assemblages differed most between mixed peat swamp and low pole. Morphological data were compared between suborders and habitats. Anisoptera showed significantly larger thoraces, hindwings, and hindwing-to-body ratio than Zygoptera. Anisoptera in low pole were significantly smaller in body, thorax, and hindwing compared to both kerangas and mixed peat swamp. Anisoptera showed a strong association with pools and Zygoptera with flowing water. Heterogeneity, habitat characteristics, presence of specialists, body size, and the interaction between species’ morphological traits and habitat characteristics likely explained the trends observed. Full article

Species with similar resource requirements cannot coexist for long. To facilitate co-existence, carnivores adapt different strategies to partition the available resources in space and time. In high-altitude ecosystems with limited resources the operation becomes complex. We assessed species diversity and spatio-temporal resource partitioning among felid species inhabiting the intricate habitats of Khangchendzonga Biosphere Reserve (KBR), Eastern Himalaya. We used systematic camera trap surveys along with abundance and overlap indices to generate information on their space use and activity patterns. We recorded six species of felids belonging to five genera, including four species of global conservation importance. The differential use of space by felids was observed with respect to the habitat types determined by sharp altitudinal gradients with varied levels of spatial and altitudinal overlap. Maximum overlap was observed among Asiatic golden cat Catopuma temminckii, jungle cat Felis chaus and clouded leopard Neofelis nebulosa. The felids had distinct activity profiles from one other, particularly between golden cat and leopard cat Prionailurus bengalensis. Pianka’s index also provided weak evidence for temporal overlap among species. The overall results indicate that habitat heterogeneity facilitates primary separation at the spatial scale, although species are also segregated temporally. Golden cat was the most adaptive felid in the area, depicting a peculiar pattern of spatio-temporal segregation with other species. We suspect either intense competition or dietary segregation among some species, and propose a further investigation of their diet and the activity patterns of their prey. The study presents an early template of carnivore community organization in resource-scarce high-altitude environments, thus, offering huge ecological and conservation significance. Full article

In recent decades, sustainable forest management has been increasingly recognized, promoting the diffusion of silvicultural practices aimed at considering all components of the forest system. Deadwood is an important component of the forest ecosystem. It plays a fundamental role in providing nutrients and habitats for a wide variety of saprotrophic and heterotrophic organisms and significantly contributes to soil formation and carbon storage. Deadwood is inhabited by a plethora of organisms from various kingdoms that have evolved the ability to utilize decaying organic matter. This community, consisting of both eukaryotic and prokaryotic species, can be defined as “necrobiome”. Through the interactions between its various members, the necrobiome influences the decay rates of deadwood and plays a crucial role in the balance between organic matter decomposition, carbon sequestration, and gas exchanges (e.g., CO₂) with the atmosphere. The present work aims to provide an overview of the biodiversity and role of the microbial communities that inhabit deadwood and their possible involvement in greenhouse gas (CO₂, N₂O, and CH₄) emissions. Full article