Harmful cyanobacterial blooms of the toxin-producing Microcystis have become a growing problem for Southwest Florida freshwater bodies. Recently, a 2016 bloom in Lake Okeechobee and a 2018 bloom in the Caloosahatchee River both led to the declaration of a state of emergency for the state of Florida. Fast-acting suppression methods are needed to protect residents and wildlife. Hydrogen peroxide and L-lysine have shown promising results in selectively inhibiting the growth of Microcystis aeruginosa and are more ecologically friendly due to fast degradation in water or the biological enhancement of nontarget organisms, respectively. We further explored the use of hydrogen peroxide, L-lysine, and combined treatments of both chemicals, which have never been tested before, for the rapid suppression of Microcystis. We assessed the susceptibility of seven M. aeruginosa strains and six other phytoplankton (Cyanobium spp., Synechococcus sp., Dolichospermum planctonica, Mychonastes homosphaera, and Chromochloris zofingiensis) commonly found in Florida, and revealed that susceptibility was diverse. All three treatments were effective at inhibiting the growth of M. aeruginosa, mixed treatments (16.7 mg/L hydrogen peroxide: 8 mg/L L-lysine) were most effective with a median growth inhibition ratio of 94.2% on the last day of the experiment, while hydrogen peroxide (16.7 mg/L) (83.8%) and L-lysine (8 mg/L) (78.5%) were less so. We found axenic M. aeruginosa to be significantly more sensitive to hydrogen peroxide when compared with nonaxenic strains (p < 0.01, n = 18). L-lysine was found to be significantly more toxic to M. aeruginosa than other examined cyanobacteria and chlorophyte strains at the end of the experiment (p < 0.001, n = 33), demonstrating its specificity to this cyanobacterium, while hydrogen peroxide and mixed treatments had varying effects on the other tested phytoplankton. Full article

The problem of assessing the impact of pollution in protected areas prompted us to apply a combined method of bioindication and spatial mapping of phytoplankton data from six lakes in the Burabay National Nature Park in Northern Kazakhstan. The issue of monitoring planning was quite acute for this landscape-homogeneous area among the Kulunda steppes. Phytoplankton in each of the six lakes was collected in the summer season of 2019 at a total of 54 sampling stations. In total, 139 species of algae and cyanobacteria from seven taxonomic phyla were found in the phytoplankton of the Burabay Park during the study period. Three phyla were the richest in species: diatoms, green algae, and cyanobacteria. Based on species richness, abundance, and biomass, as well as bioindicators and calculated indices of organic pollution and toxic effects, the current ecological state of the lake was assessed as being under the influence of pollution, of the mesotrophic type and with a high capacity for self-purification. Statistical mapping, calculated by the correlation of the species composition and categories of indicators, revealed the zones of anthropogenic impact located on the shores of the lake, and the water of the lakes as weakly alkaline, quality classes 2–3. An increase in the number of cyanobacteria in coastal communities was revealed, which may be associated with an increase in the biogenic load on the lake ecosystems. The results of the analysis and mapping of indicators revealed that two major factors regulated phytoplankton: salinity and organic pollution. The sources of organic pollution are mostly associated with the intake of substances from the coastal zone, where resorts, roads, and settlements are located. Full article

In this study, aquatic macroinvertebrates were used as bioindicators to determine the ecological conditions of the Lapa River, located between the municipalities of Cayey and Salinas, Puerto Rico. The water quality of the river was evaluated through the calculation of the Puerto Rico Biological Monitoring Working Party (BMWP-PR), as well as its degree of contamination using the Puerto Rico Family Biotic Index (FBI-PR). Bimonthly sampling was conducted across four sampling sites for a period of 12 months. The sampling sites were positioned upstream and downstream within the nature reserve, and outside and downstream its borders. The BMWP-PR results showed that the site upstream-inside the nature reserve had good water quality, and the site downstream-inside the nature reserve had regular water quality, showing some areas with eutrophication. The water quality outside-downstream from the nature reserve was poor. The FBI-PR results showed that there was mild organic contamination inside the nature reserve, while there was substantial organic contamination in the site that was outside-downstream from the nature reserve. We concluded that the section of the river located within the nature reserve had better ecological conditions than the stretch of the river located outside-downstream of the nature reserve, because it is located within a protected area that has barely been impacted by human activity. Full article

The objective of the manuscript was to study the effects of dead wood on macroinvertebrates (MI) assemblages occupying different habitat patches. Three streams were studied, with control and wooded sections in each stream from which MI were sampled for all substrate types (wood, fine detritus, gravel, sand). This contribution deals with three German streams Ihle, Nieplitz, and Verlorenwasser, which belong to the stream type 14 (sand-dominated lowland stream) and are located in the landscape unit Fläming. This type of stream, where large wood could be most influential, has been relatively understudied. This study focused on quantifying substrate distribution and macroinvertebrate taxonomy and performing statistical analysis of MI densities with regard to substrate type. The area-specific invertebrate assemblages were differentiated for MI density, dry matter density and taxonomic richness of invertebrates. In addition to macroinvertebrates, a special focus was on aquatic and semi-aquatic insects. There were significant differences between the control and wooden sections. The macroinvertebrate assemblages (individual density, dry matter density and species richness) of the detritus of the wood sections only in the Verlorenwasser was significantly and positively affected (two or three times) by the deadwood. Detritus in the wood sections of the Nieplitz and Verlorenwasser contained more sensitive species like EPT than detritus from the control sections. On the other hand, no significant difference in MI populations were determined for the sand of the wood sections compared to the sand of the control sections. In all water bodies, the wood substrate promoted all investigated indicators of macrozoobenthos as compared to sand of the wood sections. Detritus significantly promoted macrozoobenthos colonization in the wood sections as compared to sand of the Nieplitz and the Verlorenwasser. The MI density and dry matter density in the Ihle was also significantly promoted by detritus, although not significantly with regard species richness. Two significant correlations were also identified. The more sand that was present in the Ihle and the Verlorenwasser, the more the macroinvertebrate assemblages were inhibited. Additionally, the larger the area of detritus in the Ihle and the area of wood in the Nieplitz, the more MI populations were encouraged. The influence of the deadwood position on MI was also investigated, but there was no significant difference. Incline bridges, flow guides and parallel wood position types were settled in similar quality and quantity. Full article

Faunistic studies of regional biodiversity of aquatic insects are increasing in importance as declines are noted globally. Federal and state government conservation attempts for rare and threatened species are predicated upon the initial research of specialized taxonomists and trained field biologists. The reporting of aquatic insect occurrence data provides a baseline for conservation agencies to compare water quality monitoring studies. Updated field work, literature reviews, and database queries for stoneflies from the mid-Atlantic United States of America state of Maryland necessitated an assessment of species diversity for the state. Seven new state records and one new literature record are presented, bringing the total number of species to 122. Chao1 estimates of species richness are presented for diversity hotspots and the state as a whole, indicating that increased sampling is still necessary to fully understand diversity patterns. Accompanying are assessments of elevation trends and adult presence patterns within nine families. Collections are predominantly restricted to the Appalachian region. Herein, we direct future efforts to focus on understudied regions. An outline of distribution knowledge for species is presented to inform upcoming State Wildlife Action Plans. Full article

Stream ecosystems provide invaluable ecosystem services but are highly impacted ecosystems in need of water quality monitoring for habitat change impacts. Freshwater macroinvertebrate (FWI) assemblages have been shown to be good indicators of water quality and are known to be vulnerable to land-use land cover (LULC) and other habitat changes. The goal of this case study was to use an existing dominant LULC analysis in the Neponset River watershed, Massachusetts, USA, as LULC sampling treatment groups to deliberately capture the influence of these LULC effects on meso-scale habitat quality, FWI assemblages, and FWI water quality indices at eight sampling reaches. To achieve this goal, we collected physical habitat measurements and FWI samples in the summers of 2010 and 2012 at eight reach-scale stations spread across four previously determined LULC sub-watershed types (forest, residential, industrial, and golf) in the watershed. We expected that LULC change would influence the habitat quality, which would influence the FWI assemblage water quality scores and composition. We also expected that the water quality at these LULC sub-watershed types would be reflected in the FWI assemblage composition. We identified five major findings from our study. Our first finding was that the habitat quality in the Neponset River watershed was somewhat degraded relative to pristine conditions. Our second finding was that our habitat characterization analysis reflected some separation of our reach-scale macrohabitat types at land-use land-cover treatment stations with some correlations with microhabitat variables. Our third finding was that the water quality base on FWI assemblages was generally degraded in reference to pristine conditions. Our fourth finding was that, contrary to our expectations, there was no significant correlation between our reach-scale EPA habitat quality scores and FWI water quality scores. Our fifth finding was that our FWI assemblage NMS showed separation of land-use land-cover sampling stations and that that low pollution-tolerant taxa dominated some of our LULC sampling treatment stations and influenced NMS groupings. Full article

Water resources are critically important, but also pose risks of exposure to toxic and pathogenic microbes. Increasingly, a concern is toxic cyanobacteria, which have been linked to the death and disease of humans, domesticated animals, and wildlife in freshwater systems worldwide. Management approaches successful at reducing cyanobacterial abundance and toxin production have tended to be short-term solutions applied on small scales (e.g., algaecide application) or solutions that entail difficult multifaceted investments (e.g., modification of landscape and land use to reduce nutrient inputs). However, implementation of these approaches can be undermined by microbial species interactions that (a) provide toxic cyanobacteria with protection against the method of control or (b) permit toxic cyanobacteria to be replaced by other significant microbial threats. Understanding these interactions is necessary to avoid such scenarios and can provide a framework for novel strategies to enhance freshwater resource management via systems science (e.g., pairing existing physical and chemical approaches against cyanobacteria with ecological strategies such as manipulation of natural enemies, targeting of facilitators, and reduction of benthic occupancy and recruitment). Here, we review pertinent examples of the interactions and highlight potential applications of what is known. Full article