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Biosensors
Special Issues
Nanobiosensors Based on Energy Transfer
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Nanobiosensors Based on Energy Transfer

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 14468

Special Issue Editors

Dr. Karl David Wegner

E-Mail Website
Guest Editor
Division Biophotonics (BAM-1.2), Federal Institute for Materials Research and Testing (BAM), 12489 Berlin, Germany
Interests: energy transfer; quantum dots; biosensing; bioimaging; nanoparticle synthesis
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Xue Qiu

E-Mail Website
Guest Editor
School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
Interests: FRET; molecular diagnostics; miRNAs; biomarkers; neurodegenerative diseases; drug high-throughput screening

Special Issue Information

Dear Colleagues,

In the last few decades, sensing strategies based on energy transfer mechanisms, such as Förster resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET), and chemiluminescence resonance energy transfer (CRET), and also charge transfer mechanisms have enabled the highly sensitive detection of various biomolecules. Their exploitation in numerous biosensing and bioimaging applications has provided new insights into complex biological processes and significantly improved the detection limits of crucial biomarkers. In addition to conventional fluorophores, such as fluorescent dyes or proteins, new fluorescent materials, such as semiconductor nanocrystals, upconversion nanoparticles, fluorescent polymers, metal chelates, various noble metals, and other nanoparticles, have greatly fostered advances in the design of biosensors.

For this Special Issue, we seek manuscripts that use energy transfer mechanisms to design novel nanosensors for biosensing and bioimaging applications. Both reviews and original research articles will be published. Reviews should provide a critical overview of the current state-of-the-art in a particular application domain, such as a specific energy transfer mechanism, in vitro diagnostics, drug high-throughput screening, food safety and quality control, environmental pollution, or bioimaging. Reviews on the use of a specific fluorophore, such as semiconductor nanocrystals or other nanoparticles, in energy-transfer-based applications are also of interest. Original research papers that present new energy-transfer-based sensor designs and fundamental studies with potential relevance to biosensing and bioimaging are also welcome.

Dr. Karl David Wegner
Prof. Dr. Xue Qiu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • energy transfer
  • FRET/BRET/CRET
  • nanotechnology
  • nanomaterials
  • quantum dots
  • fluorescent probes
  • diagnostics
  • bioanalysis
  • point of care
  • imaging
  • food safety
  • environmental pollution
  • drug high-throughput screening

Published Papers (4 papers)

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Research

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23 pages, 18264 KiB  
Article
Resonance Energy Transfer to Track the Motion of Lanthanide Ions—What Drives the Intermixing in Core-Shell Upconverting Nanoparticles?
by Philipp U. Bastian, Nathalie Robel, Peter Schmidt, Tim Schrumpf, Christina Günter, Vladimir Roddatis and Michael U. Kumke
Biosensors 2021, 11(12), 515; https://doi.org/10.3390/bios11120515 - 14 Dec 2021
Cited by 1 | Viewed by 2401
Abstract
The imagination of clearly separated core-shell structures is already outdated by the fact, that the nanoparticle core-shell structures remain in terms of efficiency behind their respective bulk material due to intermixing between core and shell dopant ions. In order to optimize the photoluminescence [...] Read more.
The imagination of clearly separated core-shell structures is already outdated by the fact, that the nanoparticle core-shell structures remain in terms of efficiency behind their respective bulk material due to intermixing between core and shell dopant ions. In order to optimize the photoluminescence of core-shell UCNP the intermixing should be as small as possible and therefore, key parameters of this process need to be identified. In the present work the Ln(III) ion migration in the host lattices NaYF4 and NaGdF4 was monitored. These investigations have been performed by laser spectroscopy with help of lanthanide resonance energy transfer (LRET) between Eu(III) as donor and Pr(III) or Nd(III) as acceptor. The LRET is evaluated based on the Förster theory. The findings corroborate the literature and point out the migration of ions in the host lattices. Based on the introduced LRET model, the acceptor concentration in the surrounding of one donor depends clearly on the design of the applied core-shell-shell nanoparticles. In general, thinner intermediate insulating shells lead to higher acceptor concentration, stronger quenching of the Eu(III) donor and subsequently stronger sensitization of the Pr(III) or the Nd(III) acceptors. The choice of the host lattice as well as of the synthesis temperature are parameters to be considered for the intermixing process. Full article
(This article belongs to the Special Issue Nanobiosensors Based on Energy Transfer)
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28 pages, 4179 KiB  
Article
Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes
by Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera and Lawrence J. Parkhurst
Biosensors 2020, 10(11), 180; https://doi.org/10.3390/bios10110180 - 19 Nov 2020
Cited by 1 | Viewed by 4040
Abstract
The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), polarization, p(t), [...] Read more.
The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), polarization, p(t), or FRET, F(t)FRET, traces at nanomolar concentrations. These kinetic measurements are critical to elucidate reaction mechanisms, structural information, and even thermodynamics. In a single detector SF, or L-configuration, the r(t), p(t), and F(t) traces are acquired by switching the orientation of the emission polarizer to collect the IVV and IVH signals however it requires two-shot experiments. In a two-detector SF, or T-configuration, these traces are collected in a single-shot experiment, but it increases the apparatus’ complexity and price. Herein, we present a single-detector dual-channel SF to obtain the F(t) and r(t) traces simultaneously, in which a photo-elastic modulator oscillates by 90° the excitation light plane at a 50 kHz frequency, and the emission signal is processed by a set of electronic filters that split it into the r(t) and F(t) analog signals that are digitized and stored into separated spreadsheets by a custom-tailored instrument control software. We evaluated the association kinetics of binary and ternary biological complexes acquired with our dual-channel SF and the traditional methods; such as a single polarizer at the magic angle to acquire F(t), a set of polarizers to track F(t), and r(t), and by energy transfer quenching, F(t)FRET. Our dual-channel SF economized labeled material and yielded rate constants in excellent agreement with the traditional methods. Full article
(This article belongs to the Special Issue Nanobiosensors Based on Energy Transfer)
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7 pages, 1735 KiB  
Communication
Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale
by Heyjin Son, Woori Mo, Jaeil Park, Joong-Wook Lee and Sanghwa Lee
Biosensors 2020, 10(11), 168; https://doi.org/10.3390/bios10110168 - 08 Nov 2020
Cited by 12 | Viewed by 3910
Abstract
Single-molecule fluorescence energy transfer (FRET) detection has become a key technique to monitor intra- and intermolecular distance changes in biological processes. As the sensitive detection range of conventional FRET pairs is limited to 3–8 nm, complement probes are necessary for extending this typical [...] Read more.
Single-molecule fluorescence energy transfer (FRET) detection has become a key technique to monitor intra- and intermolecular distance changes in biological processes. As the sensitive detection range of conventional FRET pairs is limited to 3–8 nm, complement probes are necessary for extending this typical working range. Here, we realized a single-molecule FRET assay for a short distance range of below 3 nm by using a Cy2–Cy7 pair having extremely small spectral overlap. Using two DNA duplexes with a small difference in the labeling position, we demonstrated that our assay can observe subtle changes at a short distance range. High sensitivity in the range of 1–3 nm and compatibility with the conventional FRET assay make this approach useful for understanding dynamics at a short distance. Full article
(This article belongs to the Special Issue Nanobiosensors Based on Energy Transfer)
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Review

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25 pages, 10272 KiB  
Review
Luminescent Lifetime Regulation of Lanthanide-Doped Nanoparticles for Biosensing
by Mingkai Wang, Chuanyu Hu and Qianqian Su
Biosensors 2022, 12(2), 131; https://doi.org/10.3390/bios12020131 - 19 Feb 2022
Cited by 16 | Viewed by 3478
Abstract
Lanthanide-doped nanoparticles possess numerous advantages including tunable luminescence emission, narrow peak width and excellent optical and thermal stability, especially concerning the long lifetime from microseconds to milliseconds. Differing from other shorter-lifetime fluorescent nanomaterials, the long lifetime of lanthanide-doped nanomaterials is independent with background [...] Read more.
Lanthanide-doped nanoparticles possess numerous advantages including tunable luminescence emission, narrow peak width and excellent optical and thermal stability, especially concerning the long lifetime from microseconds to milliseconds. Differing from other shorter-lifetime fluorescent nanomaterials, the long lifetime of lanthanide-doped nanomaterials is independent with background fluorescence interference and biological tissue depth. This review presents the recent advances in approaches to regulating the lifetime and applications of bioimaging and biodetection. We begin with the introduction of the strategies for regulating the lifetime by modulating the core–shell structure, adjusting the concentration of sensitizer and emitter, changing energy transfer channel, establishing a fluorescence resonance energy transfer pathway and changing temperature. We then summarize the applications of these nanoparticles in biosensing, including ion and molecule detecting, DNA and protease detection, cell labeling, organ imaging and thermal and pH sensing. Finally, the prospects and challenges of the lanthanide lifetime regulation for fundamental research and practical applications are also discussed. Full article
(This article belongs to the Special Issue Nanobiosensors Based on Energy Transfer)
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