Special Issue "New Techniques for Extraction, Assay, and Imaging of Antioxidants"

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Extraction and Industrial Applications of Antioxidants".

Deadline for manuscript submissions: 31 March 2024 | Viewed by 413

Special Issue Editors

Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
Interests: quinone redox cycle; antioxidant assay and imaging; fluorogenic reagent; chemisluminscence reagents; food analysis; chromatographic separation of antioxidants
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
Interests: quinone redox cycle; antioxidant imaging; chemisluminscence reagents; food analysis; pharmaceutical analysis; analysis of oxidative stress biomarkers such as aldehydes

Special Issue Information

Dear Colleagues,

Antioxidant substances have garnered significant attention due to their superior ability to prevent biological components from oxidizing and to protect the body against harmful and carcinogenic reactive oxygen species. Thus, antioxidants are essential for sustaining human health. Antioxidants were found to have a protective role against many diseases, including cancers, cardiovascular diseases, and neurological diseases. Currently, various natural foods are ingested due to their nutritional antioxidant value, and many dietary supplements containing antioxidants are on the market. Therefore, a simple and rapid method for the assay, evaluation, and even imaging of antioxidant capacity in food samples and biological specimens would be useful to assess the performance of dietary supplements in vitro and in vivo and discover new effective antioxidants in natural foods.

Currently, there are many reported antioxidant extraction and assays in the literature; however, they suffer from various drawbacks, including low sensitivity, bad selectivity, long analysis time, poor extraction efficiency, the use of complicated instruments, or the use of electrochemical sensors that are deactivated by complex matrices. Hence, new innovative methods for the extraction, assay, and imaging of antioxidants in food samples and biological specimens and cells that overcome the drawback of the methods in the literature are urgently needed.

This Special Issue, entitled “New Techniques for Extraction, Assay, and Imaging of Antioxidants”, seeks papers that focus on the development of new extraction, assay, and imaging techniques for antioxidants in food samples and biological specimens. Authors are invited to submit articles focused on new and efficient extraction techniques of antioxidants, simple and rapid antioxidant assays, and efficient and sensitive antioxidant imaging. Papers on the characterization and evaluation of natural antioxidants from different food sources and manuscripts that deal with the synthesis and evaluation of new antioxidants will also be very well received.

Dr. Naoya Kishikawa
Dr. Mahmoud El-Maghrabey
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • antioxidant extraction
  • antioxidant assay
  • biological samples
  • oxidative stress status
  • nutritional value
  • food analysis
  • redox cycle
  • cell imaging

Published Papers (1 paper)

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20 pages, 1602 KiB  
Enhancing Anthocyanin Extraction from Wine Lees: A Comprehensive Ultrasound-Assisted Optimization Study
Antioxidants 2023, 12(12), 2074; https://doi.org/10.3390/antiox12122074 (registering DOI) - 05 Dec 2023
Wine lees, an important by-product of the wine industry, pose a major environmental problem due to the enormous quantities of solid–liquid waste that are discarded annually without defined applications. In this study, the optimization of a method based on a Box–Behnken design with [...] Read more.
Wine lees, an important by-product of the wine industry, pose a major environmental problem due to the enormous quantities of solid–liquid waste that are discarded annually without defined applications. In this study, the optimization of a method based on a Box–Behnken design with surface response has been carried out to obtain extracts with high anthocyanin content and potent antioxidant activity. Six variables have been considered: %EtOH, temperature, amplitude, cycle, pH, and ratio. The developed method exhibited important repeatability properties and intermediate precision, with less than 5% CV being achieved. Furthermore, these novel methods were successfully applied to diverse wine lees samples sourced from Cabernet Sauvignon and Syrah varieties (Vitis vinifera), resulting in extracts enriched with significant anthocyanin content and noteworthy antioxidant activity. Additionally, this study evaluated the influence of grape variety, fermentation type (alcoholic or malolactic), and sample treatment on anthocyanin content and antioxidant activity, providing valuable insights for further research and application in various sectors. The potential applications of these high-quality extracts extend beyond the winemaking industry, holding promise for fields like medicine, pharmaceuticals, and nutraceuticals, thus promoting a circular economy and mitigating environmental contamination. Full article
(This article belongs to the Special Issue New Techniques for Extraction, Assay, and Imaging of Antioxidants)
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