Challenges Facing the Double Haploids Production of Cereal Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (30 April 2021) | Viewed by 6397

Special Issue Editors


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Guest Editor
Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatisation Institute – NRI, 05-870 Błonie, Radzików, Poland
Interests: cereal androgenesis; tissue culture optimisation; androgenesis in cereal breeding;

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Guest Editor
Plant Biochemistry and Physiology, Plant Breeding and Acclimatisation Institute—NRI, 05-870 Błonie, Radzików, Poland
Interests: tissue culture; plant regeneration; mechanisms involved in plant regeneration; epigenetics; QTL (gene) identification; evaluation of molecular markers linked to agronomic traits

Special Issue Information

Dear Colleagues,

We invite you to submit articles on the improvement of the regeneration efficiency of plants obtained from cereal microspores.

Why we consider this topic to be particularly important:

Conventional methods for raising yields are reaching their limits, and it seems that modern biotechnology could offer new promising solutions to use biological potential in plant breeding. Double haploids are an initial and essential element of published hybrid breeding strategies in cereals.

The subject matter is of great importance for the development of basic sciences, and makes it possible to increase plant breeding efficiency, thus affecting the efficiency of agriculture, including new trends such as organic and sustainable agriculture. 

Research problems:

  1. Genetic determinants affecting in vitro regeneration.
  2. Improvement of regeneration conditions from induced embryos.
  3. Anther culture versus isolated microspore culture.
  4. Genetic stability of regenerants and their progeny.

Despite many years of work on inducing doubled haploids, the problem of genotypic dependence remains unsolved. Model genotypes are emerging, but the methods developed cannot be applied to any genotype.

Because of the above, we would most willingly accept texts on model genotypes that give multiple regenerations in in vitro cultures and papers describing androgenesis for a wide range of genotypes—especially in recalcitrant species, which include oats, wheat, and rye. However, we are also interested in articles dealing with the influence of external factors such as medium nutrient components, reprogramming stress, or light.

More effort is needed to fully understand the regulation of morphogenic response and develop methods for recalcitrant genotypes regeneration. 

Prof. Dr. Janusz Zimny
Prof. Dr. Piotr Tomasz Bednarek
Guest Editors

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Keywords

  • cereal androgenesis
  • in vitro tissue culture
  • stressful conditions
  • genotypic dependence
  • mechanisms of in vitro plant regeneration

Published Papers (1 paper)

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Research

16 pages, 4049 KiB  
Article
Improved Anther Culture Media for Enhanced Callus Formation and Plant Regeneration in Rice (Oryza sativa L.)
by Jauhar Ali, Katrina Leslie C. Nicolas, Shahana Akther, Azerkhsh Torabi, Ali Akbar Ebadi, Corinne M. Marfori-Nazarea and Anumalla Mahender
Plants 2021, 10(5), 839; https://doi.org/10.3390/plants10050839 - 22 Apr 2021
Cited by 21 | Viewed by 5545
Abstract
Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study [...] Read more.
Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study was conducted to determine the effects of two improved anther culture media, Ali-1 (A1) and Ali-2 (A2), a modified N6 medium, to enhance the callus formation and plant regeneration of japonica, indica, and hybrids of indica and japonica cross. The current study demonstrated that genotype and media had a significant impact (p < 0.001) on both callus induction frequency and green plantlet regeneration efficiency. The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480. However, the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively. Furthermore, it substantially reduced the albino plantlet regeneration of the induced callus in two hybrids (CXY6 and Y2). Therefore, the improved anther culture medium A1 can produce doubled haploid rice plants for indica × japonica, which can be useful in different breeding programs that will enable the speedy development of rice varieties for resource-poor farmers. Full article
(This article belongs to the Special Issue Challenges Facing the Double Haploids Production of Cereal Plants)
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