Plant Tissue Culture and Secondary Metabolites

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 2641

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Guest Editor
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup 56212, Republic of Korea
Interests: plant tissue culture; hairy roots; secondary metabolites; metabolic profiling; antioxidant; antimicrobial

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Guest Editor
Department of Crop Science, Chungnam National University, Daejeon 34134, Republic of Korea
Interests: plant tissue culture; hairy roots; secondary metabolites; metabolic profiling
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Dear Colleagues,

Agrobacterium rhizogenes-mediated transformation of a variety of hairy roots from many plant species has resulted in genetic stability, rapid root growth, and biosynthesis of bioactive compounds. Indeed, hairy roots have been obtained from many plant sources for the production of most groups of secondary metabolites, including phenolics, alkaloids, glucosinolates, terpenoids, and others. Plant secondary metabolites are molecules that are not essential to plant survival but have important plant development, growth, reproduction, and protection roles. These are widely distributed in higher plant species and possess biological properties associated with plant defense against biological and physical stresses. Furthermore, the intake of edible plants containing many secondary metabolites is beneficial to human health due to their biological activities, and anti-allergic, anti-cancer, anti-microbial, and antioxidant properties.

Dr. Hyeonji Yeo
Dr. Sangun Park
Guest Editors

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Keywords

  • plant tissue culture
  • hairy roots
  • Agrobacterium rhizogenes
  • secondary metabolites
  • metabolic profiling
  • antioxidant
  • antimicrobial

Published Papers (2 papers)

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Research

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12 pages, 6606 KiB  
Article
Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
by Minsol Choi, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim and Sang Un Park
Plants 2023, 12(22), 3840; https://doi.org/10.3390/plants12223840 - 13 Nov 2023
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Abstract
Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and [...] Read more.
Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and evaluated its antioxidant activities. Rosmarinic acid and five phenylpropanoids were detected using high-performance liquid chromatography. The hairy roots grown in 1/2 SH medium with 1 mg/L NAA had a high level of rosmarinic acid content. Hairy roots cultured in 1 mg/L NAA had the highest total content of five phenylpropanoids. Compared to wild-type roots grown in the field, hairy roots (NAA 1) expressed similar levels of rosmarinic acid but significantly enhanced phenylpropanoid accumulation. Furthermore, the total phenolic content and total flavonoid content of hairy roots (NAA 1) were 2.22 and 1.73 times higher than those of wild-type roots. In the results of DPPH, ABTS, and reducing power assays, the hairy roots (NAA 1) showed higher free radical scavenging effects and reduction potential than the wild-type roots. These results suggest that S. plebeia hairy roots cultured under optimal conditions, which exhibit enhanced phenolic compound accumulation and antioxidant activity, can potentially be used as sources of antioxidants. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Secondary Metabolites)
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Review

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24 pages, 1948 KiB  
Review
Anthocyanin Production from Plant Cell and Organ Cultures In Vitro
by Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek and So-Young Park
Plants 2024, 13(1), 117; https://doi.org/10.3390/plants13010117 - 31 Dec 2023
Cited by 1 | Viewed by 1436
Abstract
Anthocyanins are water-soluble pigments found in plants. They exist in various colors, including red, purple, and blue, and are utilized as natural colorants in the food and cosmetics industries. The pharmaceutical industry uses anthocyanins as therapeutic compounds because they have several medicinal qualities, [...] Read more.
Anthocyanins are water-soluble pigments found in plants. They exist in various colors, including red, purple, and blue, and are utilized as natural colorants in the food and cosmetics industries. The pharmaceutical industry uses anthocyanins as therapeutic compounds because they have several medicinal qualities, including anti-obesity, anti-cancer, antidiabetic, neuroprotective, and cardioprotective effects. Anthocyanins are conventionally procured from colored fruits and vegetables and are utilized in the food, pharmaceutical, and cosmetic industries. However, the composition and concentration of anthocyanins from natural sources vary quantitively and qualitatively; therefore, plant cell and organ cultures have been explored for many decades to understand the production of these valuable compounds. A great deal of research has been carried out on plant cell cultures using varied methods, such as the selection of suitable cell lines, medium optimization, optimization culture conditions, precursor feeding, and elicitation for the production of anthocyanin pigments. In addition, metabolic engineering technologies have been applied for the hyperaccumulation of these compounds in varied plants, including tobacco and arabidopsis. In this review, we describe various strategies applied in plant cell and organ cultures for the production of anthocyanins. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Secondary Metabolites)
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