Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
Abstract
:1. Introduction
2. Results
2.1. Hairy Root Growth in Different Media and Auxins
2.2. Analysis of Rosmarinic Acid in S. plebeia Hairy Roots
2.3. Analysis of Phenylpropanoids in S. plebeia Hairy Roots
2.4. Comparison of Metabolites in Hairy Roots and Wild-Type Roots of S. plebeia
2.5. Total Phenolic Content and Total Flavonoid Content
2.6. In Vitro Antioxidant Assays
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Hairy Root Induction and Cultures Using A. rhizogenes
4.3. Preparation of S. plebeia Extracts
4.4. HPLC Analysis of Rosmarinic Acids
4.5. HPLC Analysis of Phenylpropanoids
4.6. Determination of Total Phenolic and Flavonoid Contents
4.7. Determination of DPPH Free Radical Scavenging
4.8. Determination of ABTS Free Radical Scavenging
4.9. Determination of Reducing Power
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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p-Hydroxybenzoic Acid | Caffeic Acid | Ferulic Acid | t-Cinnamic Acid | Quercetin | Total | |
---|---|---|---|---|---|---|
B5 | 2.39 ± 0.27 a,1 | 7.44 ± 0.16 de | 1.34 ± 0.28 ab | 0.41 ± 0.03 a | 35.35 ± 0.13 b | 46.93 ± 0.6 b |
1/2 B5 | 1.98 ± 0.08 b | 8.48 ± 0.25 c | 0.47 ± 0.29 ab | 0.32 ± 0.05 abc | 35.11 ± 0.13 b | 46.35 ± 0.25 bc |
MS | 2.72 ± 0.04 a | 7.26 ± 0.17 e | 1.12 ± 0.08 a | 0.25 ± 0.01 b | 35.38 ± 0.29 b | 46.73 ± 0.16 b |
1/2 MS | 1.83 ± 0.09 b | 7.81 ± 0.05 d | 1.46 ± 0.17 a | 0.16 ± 0 c | 34.12 ± 0.18 c | 45.38 ± 0.2 c |
SH | 0.89 ± 0.14 c | 8.99 ± 0.19 b | 0.11 ± 0.04 b | 0.15 ± 0.04 bc | 35.24 ± 0.07 b | 45.39 ± 0.39 c |
1/2 SH | 1.1 ± 0.09 c | 11.47 ± 0.11 a | 0.09 ± 0.02 b | 0.19 ± 0.02 bc | 36.28 ± 0.28 a | 49.13 ± 0.41 a |
p-Hydroxybenzoic Acid | Caffeic Acid | Ferulic Acid | t-Cinnamic Acid | Quercetin | Total | |
---|---|---|---|---|---|---|
Control | 1.16 ± 0.14 bc,1 | 14.42 ± 0.13 d | 0.2 ± 0.06 bc | 0.05 ± 0.02 c | 36.08 ± 0.1 cd | 51.9 ± 0.14 f |
NAA 0.1 | 0.97 ± 0.13 bcd | 17.47 ± 0.52 bc | 0.57 ± 0.34 abc | 0.13 ± 0.07 abc | 35.8 ± 0.15 d | 54.93 ± 0.59 bcdef |
NAA 0.5 | 0.86 ± 0.13 def | 17.86 ± 0.2 b | 0.96 ± 0.07 a | 0.13 ± 0.04 abc | 35.13 ± 0.09 e | 54.94 ± 0.18 b |
NAA 1 | 1.24 ± 0.06 b | 22.3 ± 0.05 a | 1.02 ± 0.07 a | 0.14 ± 0.03 bc | 34.84 ± 0.14 e | 59.55 ± 0.08 a |
IAA 0.1 | 1.73 ± 0.15 a | 15.68 ± 0.3 cd | 0.92 ± 0.06 a | 0.28 ± 0.02 a | 36.67 ± 0.23 b | 55.28 ± 0.55 bc |
IAA 0.5 | 1.01 ± 0.04 bcd | 14.44 ± 0.12 d | 0.29 ± 0.07 bc | 0.26 ± 0.07 abc | 36.41 ± 0.05 bc | 52.43 ± 0.13 ef |
IAA 1 | 0.67 ± 0.07 ef | 16.27 ± 0.14 c | 0.38 ± 0.04 b | 0.11 ± 0.01 bc | 35.95 ± 0.11 d | 53.38 ± 0.15 cd |
IBA 0.1 | 0.58 ± 0.06 f | 15.95 ± 0.05 c | 0.5 ± 0.07 bc | 0.13 ± 0.01 bc | 36.69 ± 0.18 b | 53.84 ± 0.11 c |
IBA 0.5 | 0.89 ± 0.09 cde | 14.38 ± 0.14 d | 0.37 ± 0.1 bc | 0.21 ± 0.03 ab | 37.28 ± 0.03 a | 53.14 ± 0.18 cde |
IBA 1 | 0.81 ± 0.07 def | 13.36 ± 0.09 e | 0.16 ± 0.03 c | 0.29 ± 0.02 a | 37.56 ± 0.13 a | 52.18 ± 0.25 def |
Wild-Type Root | Hairy Root (NAA 1) | |
---|---|---|
p-hydroxybenzoic acid | 0.83 ± 0.07 | 1.24 ± 0.06 ** |
Caffeic acid | 7.9 ± 0.35 | 22.3 ± 0.05 *** |
Ferulic acid | 0.26 ± 0.03 | 1.02 ± 0.07 *** |
t-cinnamic acid | 0.33 ± 0.05 ** | 0.14 ± 0.03 |
Quercetin | 32.24 ± 0.4 | 34.84 ± 0.14 *** |
Total | 41.57 ± 0.53 | 59.55 ± 0.08 *** |
DPPH (IC50, µg/mL) | ABTS (IC50, µg/mL) | |
---|---|---|
Wild-type root | 185.87 ± 34.05 | 605.86 ± 62.02 |
Hairy root (NAA 1) | 96.32 ± 6.45 * | 354.92 ± 18.7 ** |
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Choi, M.; Yoon, J.; Yang, S.H.; Kim, J.K.; Park, S.U. Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia. Plants 2023, 12, 3840. https://doi.org/10.3390/plants12223840
Choi M, Yoon J, Yang SH, Kim JK, Park SU. Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia. Plants. 2023; 12(22):3840. https://doi.org/10.3390/plants12223840
Chicago/Turabian StyleChoi, Minsol, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, and Sang Un Park. 2023. "Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia" Plants 12, no. 22: 3840. https://doi.org/10.3390/plants12223840