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Microorganisms
Special Issues
The Pathogenesis, Epidemiology and Diagnosis of Infectious Diseases in Livestock...
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The Pathogenesis, Epidemiology and Diagnosis of Infectious Diseases in Livestock Animals

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: 31 May 2024 | Viewed by 801

Special Issue Editors

Prof. Dr. Selwyn Arlington Headley

E-Mail Website
Guest Editor
Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Paraná, Brazil
Interests: bovine respiratory disease; diagnostic immunohistochemistry; histological patterns of infectious disease agents; malignant catarrhal fever; molecular epidemiology; pathogenesis
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Fabiano José Ferreira De Sant'Ana

E-Mail Website
Guest Editor
Laboratory of Veterinary Pathological Diagnosis, Faculty of Agronomy and Veterinary Medicine, University of Brasília, Brasília, DF, Brazil
Interests: diseases of cattle; infectious diseases of livestock animals; poxviruses; vaccinia virus; histopathology; veterinary neuropathology; pathology of reproduction

Special Issue Information

Dear Colleagues,

This Special Issue focuses on the pathogenesis, epidemiology, and diagnosis of infectious diseases in livestock animals. Infectious diseases in livestock can have a significant impact on animal health and productivity, as well as on human health and the economy. Therefore, it is crucial to understand the underlying mechanisms of these diseases and to develop effective strategies for their prevention, control, and diagnosis. For this Special Issue, we are inviting the scientific community to submit original scientific articles, reviews, opinion articles, and/or short communications from fields of research related to infectious diseases in livestock animals.

The articles in this Special Issue will cover a range of topics related to infectious diseases in livestock. They will provide insights into the pathogenesis of various infectious agents, including viruses, bacteria, fungi, protozoa, and other parasitic pathogens, and how these agents interact with their hosts to cause disease. Papers related to the epidemiology of infectious diseases in livestock that address the factors that contribute to their dissemination and the methods used to control their transmission are also welcome in this Special Issue.

In addition, this Special Issue will highlight the latest advances in the diagnosis of infectious diseases in livestock. We invite articles describing various diagnostic techniques, including traditional methods such as bacteriology, mycology, and virology, as well as newer diagnostic approaches such as molecular biology, new-generation sequencing, in situ hybridization, and immunohistochemistry. The use of these techniques in the detection, pathogenesis, and identification of infectious agents of livestock animals as well as their advantages and limitations can be presented in this Special Issue.

Overall, this Special Issue aims to provide a comprehensive overview of the current understanding of infectious diseases in livestock, as well as the tools and strategies available for their prevention, control, and diagnosis. It is a valuable resource for veterinarians, researchers, and policymakers involved in the fight against infectious diseases in livestock.

Prof. Dr. Selwyn Arlington Headley
Prof. Dr. Fabiano José Ferreira De Sant'Ana
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • pathogenesis
  • epidemiology
  • diagnosis
  • livestock animals
  • infectious diseases

Published Papers (1 paper)

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Research

11 pages, 2206 KiB  
Article
Development of a Real-Time Recombinase-Aided Amplification Method for the Rapid Detection of Streptococcus equi subsp. equi
by Haoyu Zu, Rongkuan Sun, Jiaxin Li, Xing Guo, Min Wang, Wei Guo and Xiaojun Wang
Microorganisms 2024, 12(4), 777; https://doi.org/10.3390/microorganisms12040777 - 11 Apr 2024
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Abstract
Streptococcus equi subspecies equi (S. equi) is the causative pathogen of strangles in horses, donkeys, and other equine animals. Strangles has spread globally and causes significant losses to the horse industry. In response to the urgent need for effective disease control, [...] Read more.
Streptococcus equi subspecies equi (S. equi) is the causative pathogen of strangles in horses, donkeys, and other equine animals. Strangles has spread globally and causes significant losses to the horse industry. In response to the urgent need for effective disease control, this study introduces a novel nucleic acid diagnostic method known as a real-time recombinase-assisted amplification (RAA) assay, developed based on the eqbE gene, for the rapid detection of S. equi nucleic acid. The real-time RAA method employs specifically designed probes and primers targeting the eqbE gene, enhancing the overall specificity and sensitivity of the detection. After efficiency optimization, this real-time RAA method can detect 10 or more copies of nucleic acid within 20 min. The method demonstrates high specificity for S. equi and does not cross-react with other clinically relevant pathogens. Real-time RAA diagnostic performance was evaluated using 98 nasal swab samples collected from horses and compared with the real-time PCR detection method. Results revealed that 64 and 65 samples tested positive for S. equi using real-time RAA and real-time PCR, respectively. The overall agreement between the two assays was 96.94% (95/98), with a kappa value of 0.931 (p < 0.001). Further linear regression analysis indicated a significant correlation in the detection results between the two methods (R2 = 0.9012, p < 0.0001), suggesting that the real-time RAA assay exhibits a detection performance comparable to that of real-time PCR. In conclusion, the real-time RAA assay developed here serves as a highly specific and reliable diagnostic tool for the detection of S. equi in equine samples, offering a potential alternative to real-time PCR methods. In conclusion, the real-time RAA nucleic acid diagnostic method, based on the eqbE gene, offers rapid and accurate diagnosis of S. equi, with the added advantage of minimal equipment requirements, thus contributing to the efficient detection of strangles in horses. Full article
(This article belongs to the Special Issue The Pathogenesis, Epidemiology and Diagnosis of Infectious Diseases in Livestock Animals)
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