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Metabolites
Special Issues
Metabolomic Methodology
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Metabolomic Methodology

A special issue of Metabolites (ISSN 2218-1989).

Deadline for manuscript submissions: closed (31 July 2015)

Special Issue Editor

Prof. Dr. Per Bruheim

E-Mail Website
Guest Editor
Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, N-7491 Trondheim, Norway
Interests: mass spectrometry; metabolomics; metabolic engineering; secondary metabolites; biomakers
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A metabolomic study is divided into several steps: quenching/inactivation of metabolism, metabolite extraction and sample processing, quantitative or qualitative analysis, data analysis, and finally interpretation of data in a biological context. The sampling steps must be optimized for the chosen analytical platform, and many alternative strategies have been presented. Mass spectrometry (MS) and NMR are the two main technologies employed in metabolomic studies, the former usually with chromatographic separation of the metabolites prior to MS detection. There have been significant developments in metabolomic methodology over recent years, in parallel with improvements in instrumentation. However, there must be a continuous focus on further improvement of existing methodology as well as developing new technologies. Here, we call for manuscripts that present developments any of the steps in a metabolomic study.

Dr. Per Bruheim
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • methodology
  • mass spectrometry
  • NMR, chromatography
  • quenching and extraction
  • sample processing
  • data normalization

Published Papers (1 paper)

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Research

341 KiB  
Article
An Efficient Single Phase Method for the Extraction of Plasma Lipids
by Zahir H. Alshehry, Christopher K. Barlow, Jacquelyn M. Weir, Youping Zhou, Malcolm J. McConville and Peter J. Meikle
Metabolites 2015, 5(2), 389-403; https://doi.org/10.3390/metabo5020389 - 17 Jun 2015
Cited by 118 | Viewed by 9941
Abstract
Lipidomic approaches are now widely used to investigate the relationship between lipid metabolism, health and disease. Large-scale lipidomics studies typically aim to quantify hundreds to thousands of lipid molecular species in a large number of samples. Consequently, high throughput methodology that can efficiently [...] Read more.
Lipidomic approaches are now widely used to investigate the relationship between lipid metabolism, health and disease. Large-scale lipidomics studies typically aim to quantify hundreds to thousands of lipid molecular species in a large number of samples. Consequently, high throughput methodology that can efficiently extract a wide range of lipids from biological samples is required. Current methods often rely on extraction in chloroform:methanol with or without two phase partitioning or other solvents, which are often incompatible with liquid chromatography electrospray ionization-tandem mass spectrometry (LC ESI-MS/MS). Here, we present a fast, simple extraction method that is suitable for high throughput LC ESI-MS/MS. Plasma (10 μL) was mixed with 100 μL 1-butanol:methanol (1:1 v/v) containing internal standards resulting in efficient extraction of all major lipid classes (including sterols, glycerolipids, glycerophospholipids and sphingolipids). Lipids were quantified using positive-ion mode LC ESI-MS/MS. The method showed high recovery (>90%) and reproducibility (%CV < 20%). It showed a strong correlation of all lipid measures with an established chloroform:methanol extraction method (R2 = 0.976). This method uses non-halogenated solvents, requires no drying or reconstitution steps and is suitable for large-scale LC ESI-MS/MS-based lipidomic analyses in research and clinical laboratories. Full article
(This article belongs to the Special Issue Metabolomic Methodology)
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