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Life
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Development of Genetic Engineering Technologies for Crops
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Development of Genetic Engineering Technologies for Crops

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 3913

Special Issue Editors

Dr. Elumalai Sivamani

E-Mail Website
Guest Editor
Syngenta, Research Triangle Park, NC, USA
Interests: plant cell culture; plant biotechnology; transgenic plants; plant tissue culture; genome editing; plant gene expression; plant morphogenic factors; plant growth-promoting rhizobacteria
Dr. Po-Hao Wang

E-Mail Website
Guest Editor
Plant Analysis Group, Syngenta, Research Triangle Park, NC, USA
Interests: trait (GM and gene editing) product development; gene editing; transgenic biotechnology; molecular breeding

Special Issue Information

Dear Colleagues,

This Special Issue on the Development of Genetic Engineering Technologies for Crops published in Life is dedicated to original research or review articles from global researchers on the technologies used to genetically engineer crops. In this Special Issue, we aim to publish high-impact original research and critical reviews by leading researchers in the area of applied plant science, with an emphasis on modern genetic engineering technologies and their applications to improve crops. We aim to provide a forum for the most important advances in this field, including hypothesis- and data-driven studies with the potential for application in plant genetic and genome engineering. To be accepted for publication in this Special Issue, original research papers will need to present new findings with conclusions supported by critical experimental evidence and make a substantial contribution to genetic engineering technologies. Reviews must provide critical high-level insight beyond just a summary of published work. Applications may involve agriculture, horticulture, forestry, biodiversity and conservation, improved foods and food processing. Examples of the areas covered in this Special Issue include the following: new transgenic and genome editing technologies; production and analysis of transgenic crops with novel traits; gene insertion or deletion, expression and silencing; technologies used to improve foods, increase shelf life, etc. 

In this Special Issue, the Editors seek to achieve a fast review process to provide authors with constructive critical reviews for rapid publication. 

Dr. Elumalai Sivamani
Dr. Po-Hao Wang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • agrobacterium-mediated transformation
  • gene gun
  • biolistic particle-mediated gene delivery
  • plant growth regulators
  • plant morphogenic factors
  • plant biotechnology
  • applied plant science
  • plant biology
  • agriculture
  • horticulture
  • food
  • food processing
  • food safety
  • pharmaceuticals
  • cell biology
  • gene and genome engineering and analysis
  • transgenic crops
  • gene silencing
  • gene expression
  • modified crops
  • secondary metabolites
  • manipulations of plant physiology
  • plant developmental studies
  • plant developmental mechanisms

Published Papers (2 papers)

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Research

12 pages, 2448 KiB  
Article
Emergence of Rice Blast AVR-Pi9 Resistance Breaking Haplotypes in Yunnan Province, China
by Lin Lu, Qun Wang, Zhufeng Shi, Chengyun Li, Zhixiang Guo and Jinbin Li
Life 2023, 13(6), 1320; https://doi.org/10.3390/life13061320 - 04 Jun 2023
Cited by 1 | Viewed by 1299
Abstract
The rice blast disease (caused by Magnaporthe oryzae) is a devastating disease in China. Understanding the molecular mechanisms of interaction for the cognate avirulence (AVR) gene with host resistance (R) genes, as well as their genetic evolution is [...] Read more.
The rice blast disease (caused by Magnaporthe oryzae) is a devastating disease in China. Understanding the molecular mechanisms of interaction for the cognate avirulence (AVR) gene with host resistance (R) genes, as well as their genetic evolution is essential for sustainable rice production. In the present study, we conducted a high-throughput nucleotide sequence polymorphism analysis of the AVR-Pi9 gene that was amplified from the rice-growing regions of the Yunnan Province in China. We detected the presence of seven novel haplotypes from 326 rice samples. In addition, the sequences of AVR-Pi9 were also obtained from two non-rice hosts, Eleusine coracana and Eleusine indica. The sequence analysis revealed the insertions and deletions in the coding and non-coding regions of the gene. The pathogenicity experiments of these haplotypes on previously characterized monogenic lines showed that the newly identified haplotypes are virulent in nature. The breakdown of resistance was attributed to the development of new haplotypes. Our results suggest that the mutation in the AVR-Pi9 gene is an alarming situation in the Yunnan province and thus needs attention. Full article
(This article belongs to the Special Issue Development of Genetic Engineering Technologies for Crops)
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20 pages, 2737 KiB  
Article
Impact of Light and Dark Treatment on Phenylpropanoid Pathway Genes, Primary and Secondary Metabolites in Agastache rugosa Transgenic Hairy Root Cultures by Overexpressing Arabidopsis Transcription Factor AtMYB12
by Thi Minh Hanh Do, Minsol Choi, Jae Kwang Kim, Ye Jin Kim, Chanung Park, Chang Ha Park, Nam Il Park, Changsoo Kim, Ramaraj Sathasivam and Sang Un Park
Life 2023, 13(4), 1042; https://doi.org/10.3390/life13041042 - 19 Apr 2023
Cited by 2 | Viewed by 1915
Abstract
Agastache rugosa, otherwise called Korean mint, has a wide range of medicinal benefits. In addition, it is a rich source of several medicinally valuable compounds such as acacetin, tilianin, and some phenolic compounds. The present study aimed to investigate how the Tartary [...] Read more.
Agastache rugosa, otherwise called Korean mint, has a wide range of medicinal benefits. In addition, it is a rich source of several medicinally valuable compounds such as acacetin, tilianin, and some phenolic compounds. The present study aimed to investigate how the Tartary buckwheat transcription factor AtMYB12 increased the primary and secondary metabolites in Korean mint hairy roots cultured under light and dark conditions. A total of 50 metabolites were detected by using high-performance liquid chromatography (HPLC) and gas chromatography–time-of-flight mass spectrometry (GC-TOFMS). The result showed that the AtMYB12 transcription factor upregulated the phenylpropanoid biosynthesis pathway genes, which leads to the highest accumulation of primary and secondary metabolites in the AtMYB12-overexpressing hairy root lines (transgenic) than that of the GUS-overexpressing hairy root line (control) when grown under the light and dark conditions. However, when the transgenic hairy root lines were grown under dark conditions, the phenolic and flavone content was not significantly different from that of the control hairy root lines. Similarly, the heat map and hierarchical clustering analysis (HCA) result showed that most of the metabolites were significantly abundant in the transgenic hairy root cultures grown under light conditions. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) showed that the identified metabolites were separated far based on the primary and secondary metabolite contents present in the control and transgenic hairy root lines grown under light and dark conditions. Metabolic pathway analysis of the detected metabolites showed 54 pathways were identified, among these 30 were found to be affected. From these results, the AtMYB12 transcription factor activity might be light-responsive in the transgenic hairy root cultures, triggering the activation of the primary and secondary metabolic pathways in Korean mint. Full article
(This article belongs to the Special Issue Development of Genetic Engineering Technologies for Crops)
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