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State-of-the-Art Bioactives and Nutraceuticals in Spain
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State-of-the-Art Bioactives and Nutraceuticals in Spain (Closed)

A topical collection in International Journal of Molecular Sciences (ISSN 1422-0067). This collection belongs to the section "Bioactives and Nutraceuticals".

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Editors

Prof. Dr. Jesús Osada

E-Mail Website
Collection Editor
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain
Interests: transcriptomics; diet; medicinal plant
Special Issues, Collections and Topics in MDPI journals
Dr. Rafael A. Casuso

E-Mail Website
Collection Editor
Department of Physiology, Institute of Nutrition and Food Technology, University of Granada, 18010 Granada, Spain
Interests: mitochondria; oxidative stress; exercise physiology; skeletal muscle
Special Issues, Collections and Topics in MDPI journals
Dr. Iñaki Milton-Laskibar

E-Mail Website
Collection Editor
1. Cardiometabolic Nutrition Group, IMDEA Food Institute, 28049 Madrid, Spain
2. CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, 28029 Madrid, Spain
Interests: obesity; diabetes; NAFLD; lipid metabolism; autophagy; mitochondria; polyphenols
Special Issues, Collections and Topics in MDPI journals

Topical Collection Information

Dear Colleagues,

This Collection aims to publish contributions that report on novel research findings regarding bioactive compounds and nutraceutical products in Spain. We welcome submissions and studies on nutritional applications using biological, chemical, cellular, molecular, and immunological methods. Topics include, but are not limited to:

  • the discovery of novel bioactive natural products
  • the role of these products in manipulating food structure and hence potential physiological mediation for human nutrition
  • the use of in vitro and in vivo bioactivity research using cell lines and animal models as exemplars of human physiology

The only limitation is that the main part of the study has to have been carried out in Spain or by Spanish researchers.

Importantly, the exact active ingredient of natural origin extract must be reported in the submitted research manuscript, since papers describing the effects of mixed extraction from natural origin are not in the scope of the journal.

Prof. Dr. Jesús Osada
Dr. Rafael A. Casuso
Dr. Iñaki Milton-Laskibar
Collection Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive
  • nutraceutical
  • nutrient
  • health
  • diet
  • functional food
  • obesity
  • diabetes
  • cancer
  • cardiovascular and cerebrovascular diseases
  • vitamins
  • proteins
  • peptides
  • polysaccharides
  • carotenoids
  • polyphenols
  • phytosterols and isoflavones
  • saponins
  • phytic acid
  • probiotics
  • prebiotics
  • enzymes
  • flavonoids
  • caffeine
  • carnitine
  • choline
  • creatine
  • dithiolthiones
  • phytoestrogens
  • glucosinolates, etc.

Published Papers (5 papers)

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2023

Jump to: 2022

20 pages, 3467 KiB  
Article
Differentially Expressed Genes in Response to a Squalene-Supplemented Diet Are Accurate Discriminants of Porcine Non-Alcoholic Steatohepatitis
by Roubi Abuobeid, Luis V. Herrera-Marcos, Carmen Arnal, Seyed Hesamoddin Bidooki, Javier Sánchez-Marco, Roberto Lasheras, Joaquín C. Surra, María Jesús Rodríguez-Yoldi, Roberto Martínez-Beamonte and Jesús Osada
Int. J. Mol. Sci. 2023, 24(16), 12552; https://doi.org/10.3390/ijms241612552 - 08 Aug 2023
Cited by 1 | Viewed by 1455
Abstract
Squalene is the major unsaponifiable component of virgin olive oil, the fat source of the Mediterranean diet. To evaluate its effect on the hepatic transcriptome, RNA sequencing was carried out in two groups of male Large White x Landrace pigs developing nonalcoholic steatohepatitis [...] Read more.
Squalene is the major unsaponifiable component of virgin olive oil, the fat source of the Mediterranean diet. To evaluate its effect on the hepatic transcriptome, RNA sequencing was carried out in two groups of male Large White x Landrace pigs developing nonalcoholic steatohepatitis by feeding them a high fat/cholesterol/fructose and methionine and choline-deficient steatotic diet or the same diet with 0.5% squalene. Hepatic lipids, squalene content, steatosis, activity (ballooning + inflammation), and SAF (steatosis + activity + fibrosis) scores were analyzed. Pigs receiving the latter diet showed hepatic squalene accumulation and twelve significantly differentially expressed hepatic genes (log2 fold change < 1.5 or <1.5) correlating in a gene network. These pigs also had lower hepatic triglycerides and lipid droplet areas and higher cellular ballooning. Glutamyl aminopeptidase (ENPEP) was correlated with triglyceride content, while alpha-fetoprotein (AFP), neutralized E3 ubiquitin protein ligase 3 (NEURL3), 2′-5′-oligoadenylate synthase-like protein (OASL), and protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B) were correlated with activity reflecting inflammation and ballooning, and NEURL3 with the SAF score. AFP, ENPEP, and PPP1R1B exhibited a remarkably strong discriminant power compared to those pathological parameters in both experimental groups. Moreover, the expression of PPP1R1B, TMEM45B, AFP, and ENPEP followed the same pattern in vitro using human hepatoma (HEPG2) and mouse liver 12 (AML12) cell lines incubated with squalene, indicating a direct effect of squalene on these expressions. These findings suggest that squalene accumulated in the liver is able to modulate gene expression changes that may influence the progression of non-alcoholic steatohepatitis. Full article
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18 pages, 2535 KiB  
Article
Optimized De Novo Eriodictyol Biosynthesis in Streptomyces albidoflavus Using an Expansion of the Golden Standard Toolkit for Its Use in Actinomycetes
by Patricia Magadán-Corpas, Suhui Ye, Álvaro Pérez-Valero, Patrick L. McAlpine, Paula Valdés-Chiara, Jesús Torres-Bacete, Juan Nogales, Claudio J. Villar and Felipe Lombó
Int. J. Mol. Sci. 2023, 24(10), 8879; https://doi.org/10.3390/ijms24108879 - 17 May 2023
Cited by 7 | Viewed by 1874
Abstract
Eriodictyol is a hydroxylated flavonoid displaying multiple pharmaceutical activities, such as antitumoral, antiviral or neuroprotective. However, its industrial production is limited to extraction from plants due to its inherent limitations. Here, we present the generation of a Streptomyces albidoflavus bacterial factory edited at the [...] Read more.
Eriodictyol is a hydroxylated flavonoid displaying multiple pharmaceutical activities, such as antitumoral, antiviral or neuroprotective. However, its industrial production is limited to extraction from plants due to its inherent limitations. Here, we present the generation of a Streptomyces albidoflavus bacterial factory edited at the genome level for an optimized de novo heterologous production of eriodictyol. For this purpose, an expansion of the Golden Standard toolkit (a Type IIS assembly method based on the Standard European Vector Architecture (SEVA)) has been created, encompassing a collection of synthetic biology modular vectors (adapted for their use in actinomycetes). These vectors have been designed for the assembly of transcriptional units and gene circuits in a plug-and-play manner, as well as for genome editing using CRISPR-Cas9-mediated genetic engineering. These vectors have been used for the optimization of the eriodictyol heterologous production levels in S. albidoflavus by enhancing the flavonoid-3′-hydroxylase (F3’H) activity (by means of a chimera design) and by replacing three native biosynthetic gene clusters in the bacterial chromosome with the plant genes matBC (involved in extracellular malonate uptake and its intracellular activation into malonyl-CoA), therefore allowing more malonyl-CoA to be devoted to the heterologous production of plant flavonoids in this bacterial factory. These experiments have allowed an increase in production of 1.8 times in the edited strain (where the three native biosynthetic gene clusters have been deleted) in comparison with the wild-type strain and a 13 times increase in eriodictyol overproduction in comparison with the non-chimaera version of the F3′H enzyme. Full article
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2022

Jump to: 2023

15 pages, 625 KiB  
Article
25-Hydroxyvitamin D Serum Levels Linked to Single Nucleotide Polymorphisms (SNPs) (rs2228570, rs2282679, rs10741657) in Skeletal Muscle Aging in Institutionalized Elderly Men Not Supplemented with Vitamin D
by Diego Fernández-Lázaro, Juan Luis García Hernández, Eva Lumbreras, Juan Mielgo-Ayuso and Jesús Seco-Calvo
Int. J. Mol. Sci. 2022, 23(19), 11846; https://doi.org/10.3390/ijms231911846 - 06 Oct 2022
Cited by 3 | Viewed by 1774
Abstract
Sarcopenia (Sp) is the loss of skeletal muscle mass associated with aging that results in an involution of muscle function and strength. Vitamin D deficiency is a common health problem worldwide, especially among the elderly, and hypovitaminosis D leads to musculoskeletal disorders. The [...] Read more.
Sarcopenia (Sp) is the loss of skeletal muscle mass associated with aging that results in an involution of muscle function and strength. Vitamin D deficiency is a common health problem worldwide, especially among the elderly, and hypovitaminosis D leads to musculoskeletal disorders. The aim of this study was to evaluate the impact and presence of a possible linkage between Single Nucleotide Polymorphisms (SNPs) CYP2R1 (rs10741657), GC (rs2282679), and VDR (rs2228570), serum 25-OH/D concentrations and the link with the degree of sarcopenia in 19 institutionalized elderly men not supplemented with vitamin D. Levels of 25-OH vitamin D were quantified with a commercial enzyme-linked immunosorbent assay kit and 3 SNPs were genotyped with KASPar assays. Significant differences in 25-OH/D concentration were determined between the bi-allelic combinations of rs228679 and rs228570. We detected statistically significant weak positive correlations between the AA (rs10741657 and rs228570) and TT (rs228679) and alleles and 25-OH/D and the probability of having higher 25-OH/D concentrations was 2- to 3-fold higher. However, the GG alleles of the 3 SNPs showed that the probability of having optimal 25-0H/D concentrations decreases by 32% for rs10741657, 38% for rs228679, and 74% for rs228570, showing a strong negative correlation between the degree of sarcopenia and 25-OH/D levels. Allelic variations in CYP2R1 (rs10741657), GC (rs2282679), and VDR (rs10741657) affect vitamin D levels and decisively influence the degree of sarcopenia in institutionalized elderly people. Full article
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13 pages, 4674 KiB  
Article
SCA® Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells
by Begoña Castro, Naiara de Paz, Salvador González and Azahara Rodríguez-Luna
Int. J. Mol. Sci. 2022, 23(12), 6538; https://doi.org/10.3390/ijms23126538 - 10 Jun 2022
Cited by 1 | Viewed by 1998
Abstract
The identification of compounds and natural ingredients that can counteract tissue stress and dysfunction induced by aging in skin cells is warranted. Here, we investigated the activity of the secretion from the snail Cryptomphalus aspersa (SCA®), an active compound with well-established [...] Read more.
The identification of compounds and natural ingredients that can counteract tissue stress and dysfunction induced by aging in skin cells is warranted. Here, we investigated the activity of the secretion from the snail Cryptomphalus aspersa (SCA®), an active compound with well-established beneficial effects on skin integrity and aging. To determinate its senescence-regulation mechanisms, we used a model where damage was induced by hydrogen peroxide (H2O2). The results showed that SCA® positively modulated factors involved in cell senescence such as β-galactosidase and cell morphology, secretory efficiency markers (SIRT1/6 and carboxymethyl-lysine), and metabolic and redox homeostasis (mTOR and ROS). This study demonstrated a novel compound that is activity-modulating, reduces cell senescence, and increases longevity to maintain skin homeostasis and functionality. Full article
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16 pages, 3699 KiB  
Article
Squalene through Its Post-Squalene Metabolites Is a Modulator of Hepatic Transcriptome in Rabbits
by Roubi Abuobeid, Javier Sánchez-Marco, María J. Felices, Carmen Arnal, Juan Carlos Burillo, Roberto Lasheras, Rebeca Busto, Miguel A. Lasunción, María Jesús Rodríguez-Yoldi, Roberto Martínez-Beamonte and Jesús Osada
Int. J. Mol. Sci. 2022, 23(8), 4172; https://doi.org/10.3390/ijms23084172 - 10 Apr 2022
Cited by 6 | Viewed by 2205
Abstract
Squalene is a natural bioactive triterpene and an important intermediate in the biosynthesis of sterols. To assess the effect of this compound on the hepatic transcriptome, RNA-sequencing was carried out in two groups of male New Zealand rabbits fed either a diet enriched [...] Read more.
Squalene is a natural bioactive triterpene and an important intermediate in the biosynthesis of sterols. To assess the effect of this compound on the hepatic transcriptome, RNA-sequencing was carried out in two groups of male New Zealand rabbits fed either a diet enriched with 1% sunflower oil or the same diet with 0.5% squalene for 4 weeks. Hepatic lipids, lipid droplet area, squalene, and sterols were also monitored. The Squalene administration downregulated 9 transcripts and upregulated 13 transcripts. The gene ontology of transcripts fitted into the following main categories: transporter of proteins and sterols, lipid metabolism, lipogenesis, anti-inflammatory and anti-cancer properties. When the results were confirmed by RT-qPCR, rabbits receiving squalene displayed significant hepatic expression changes of LOC100344884 (PNPLA3), GCK, TFCP2L1, ASCL1, ACSS2, OST4, FAM91A1, MYH6, LRRC39, LOC108176846, GLT1D1 and TREH. A squalene-enriched diet increased hepatic levels of squalene, lanosterol, dihydrolanosterol, lathosterol, zymostenol and desmosterol. Strong correlations were found among specific sterols and some squalene-changed transcripts. Incubation of the murine AML12 hepatic cell line in the presence of lanosterol, dihydrolanosterol, zymostenol and desmosterol reproduced the observed changes in the expressions of Acss2, Fam91a1 and Pnpla3. In conclusion, these findings indicate that the squalene and post-squalene metabolites play important roles in hepatic transcriptional changes required to protect the liver against malfunction. Full article
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