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Recent Advances in Biomolecule and Bioprocess Engineering for Sustainable Environment

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 1903

Special Issue Editors


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Guest Editor
Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea
Interests: Biomaterial Applications; Biomolecule Identification; Biomass Conversion; Enzyme Technology; Applied Microbiology; Biofuel Production; Response Surface Methodology; Bioprocess Engineering; Purification; Fermentation; Process Design and Simulation
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Guest Editor
Department of Food Science and Engineering, Dongyang Mirae University, 445, Gyeongin-ro, Guro-gu, Seoul 08221, Republic of Korea
Interests: bioprocess engineering; biorefinery; biotechnology; enzyme; microalgae; biochar
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Chemical Engineering, Kwangwoon University, 20, Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea
Interests: bioactive compounds; marine biorefinery; process optimization
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Climate change caused by anthropogenic greenhouse gas emissions is a global challenge that needs to be urgently addressed. White biotechnology as an alternative to the petrochemical industry, which is known to be a major source of greenhouse gas emissions, has recently received significant research attention. We are hopeful that rapid advances in the core technologies and bioprocesses of biorefinery and their application to industry will ensure a sustainable environment and future. The biorefinery concept is based on biotechnology used to produce valuable bioproducts with carbon-neutral biomass as feedstock. The biotechnology associated with biorefineries has evolved considerably to meet the challenges of efficiently utilizing a diverse biomass. The main challenges lie in securing the economic feasibility of the bioprocess, and more specifically, in the (1) exploration, characterization, and development of strategies to utilize sustainable biomass such as lignocellulose, marine biomass, and food processing residues; (2) strain development to enhance the production of bioproducts such as enzymes, biomolecules, biochemicals, bioenergy, and biopolymers; and (3) the provision of molecular-level analyses, such as biosensing and research, to improve efficiency, such as biomass pretreatment, enzymatic conversion, carbonization, etc. Advanced biotechnology and tools such as metabolic engineering, protein engineering, enzyme engineering, bioprocess engineering, molecular analysis, and statistical optimization are expected to address these challenges.

The aim of this special issue, entitled "Recent Advances in Biomolecule and Bioprocess Engineering for Sustainable Environment" is to present new discoveries and innovations at the biomolecular level and their application to bioprocesses. Topics of interest include but are not limited to, the following:

  • Strategies for the production or development of various biomolecules (such as extraction, identification, synthesis, conversion).
  • State-of-the-art in strain development via molecular biology to improve the yield.
  • Developments in molecular sensing or molecular markers.
  • Development of biocatalysts or pretreatment processes.
  • Strategies for generational applications of biomass via chemical composition.
  • Improve biocatalytic reaction or bioprocess efficiency via statistical optimization.
  • Trends in advanced biomolecular or bioconversion technologies that can contribute to a sustainable environment.

Dr. Hah Young Yoo
Dr. Ja Hyun Lee
Prof. Dr. Chulhwan Park
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive molecule
  • biocatalyst
  • biochar
  • bioconversion
  • biomass
  • biorefinery
  • biopolymer
  • bioprocess
  • biotechnology

Published Papers (2 papers)

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Research

16 pages, 1502 KiB  
Article
Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol
by Anita Rywińska, Ludwika Tomaszewska-Hetman, Zbigniew Lazar, Piotr Juszczyk, Patrycja Sałata, Karolina Malek, Adrian Kawecki and Waldemar Rymowicz
Int. J. Mol. Sci. 2024, 25(3), 1475; https://doi.org/10.3390/ijms25031475 - 25 Jan 2024
Viewed by 818
Abstract
Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species Yarrowia lipolytica. The [...] Read more.
Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species Yarrowia lipolytica. The aim of this study was to evaluate transformants of Y. lipolytica for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among Y. lipolytica species. Full article
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22 pages, 5931 KiB  
Article
Enhanced Enzymatic Synthesis of Puerarin Palmitate with Different Acyl Donors for Lipid Solubility Improvement
by Seungmee Lee, Hyeonmi Shin, Jihyun Bae, Taek Lee, Minji Kim, Heung Bae Jeon, Kang Hyun Lee, Hah Young Yoo and Chulhwan Park
Int. J. Mol. Sci. 2024, 25(2), 709; https://doi.org/10.3390/ijms25020709 - 05 Jan 2024
Cited by 1 | Viewed by 826
Abstract
Puerarin is a flavonoid known as a natural antioxidant found in the root of Pueraria robata. Its antioxidant, anticancer, and anti-inflammatory effects have attracted attention as a potential functional ingredient in various bioindustries. However, puerarin has limited bioavailability owing to its low [...] Read more.
Puerarin is a flavonoid known as a natural antioxidant found in the root of Pueraria robata. Its antioxidant, anticancer, and anti-inflammatory effects have attracted attention as a potential functional ingredient in various bioindustries. However, puerarin has limited bioavailability owing to its low lipid solubility and stability. Acylation is proposed as a synthesis method to overcome this limitation. In this study, lipase-catalyzed acylation of puerarin and various acyl donors was performed, and the enzymatic synthetic condition was optimized. Under the condition (20 g/L of Novozym 435, palmitic anhydride, 1:15, 40 °C, tetrahydrofuran (THF)), the synthesis of puerarin ester achieved a significantly high conversion (98.97%) within a short time (3 h). The molecule of the synthesized puerarin palmitate was identified by various analyses such as liquid chromatography–mass spectrometry (LC–MS), Fourier-transform infrared spectroscopy (FT-IR), and carbon-13 nuclear magnetic resonance (13C NMR). The lipid solubility and the radical scavenging activity were also evaluated. Puerarin palmitate showed a slight decrease in antioxidant activity, but lipid solubility was significantly improved, improving bioavailability. The high conversion achieved for puerarin esters in this study will provide the foundation for industrial applications. Full article
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