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New Discoveries in Cartilage Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (28 February 2024) | Viewed by 1712

Special Issue Editor


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Guest Editor
Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, 54000 Nancy, France
Interests: natural products; bioactive compounds; phenolics; plant derived compounds; medicinal plants; natural products chemistry; pre-formulation; hydrogel; wound healing; nanoparticle; cartilage; osteoarthritis; ectopic mineralization; liposomes; extracellular vesicles; biomaterials; nuclear receptors; oxidant stress; cellular signalisation
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Special Issue Information

Dear Colleagues,

Articular cartilage is a specialized connective tissue devoid of vessels and neurons. It is composed of cells called chondrocytes that provide their own surrounding extracellular matrix (ECM). The unique mechanical properties of cartilage depend on ECM constituents.

Trauma or musculoskeletal diseases can lead to cartilage degeneration, and the entailed adverse processes remain not fully understood. To date, there is no therapy that might regenerate cartilage, and the current treatment for damaged cartilage is to handle its symptoms (joint pain and reduced joint functional capacities) to maintain the patient's quality of life. Indeed, chondrocytes have a low regenerative potential and are cells that are hard to target to deliver therapeutic agents, with their ECM being difficult to cross.

This call for papers aims to highlight the underlying mechanisms in cartilage (all articulations and intravertebral disc) physiology and pathophysiology as well as the new strategies of regenerative medicine to improve cartilage repair.

Dr. Arnaud Bianchi
Guest Editor

Manuscript Submission Information

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Keywords

  • articular cartilage
  • extracellular matrix
  • cartilage repair
  • articulations
  • intravertebral disc
  • cartilage degeneration

Published Papers (1 paper)

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Research

31 pages, 5882 KiB  
Article
Pro-Inflammatory Cytokine Priming and Purification Method Modulate the Impact of Exosomes Derived from Equine Bone Marrow Mesenchymal Stromal Cells on Equine Articular Chondrocytes
by Manon Jammes, Frédéric Cassé, Emilie Velot, Arnaud Bianchi, Fabrice Audigié, Romain Contentin and Philippe Galéra
Int. J. Mol. Sci. 2023, 24(18), 14169; https://doi.org/10.3390/ijms241814169 - 16 Sep 2023
Cited by 1 | Viewed by 1403
Abstract
Osteoarthritis (OA) is a widespread osteoarticular pathology characterized by progressive hyaline cartilage degradation, exposing horses to impaired well-being, premature career termination, alongside substantial financial losses for horse owners. Among the new therapeutic strategies for OA, using mesenchymal stromal cell (MSC)-derived exosomes (MSC-exos) appears [...] Read more.
Osteoarthritis (OA) is a widespread osteoarticular pathology characterized by progressive hyaline cartilage degradation, exposing horses to impaired well-being, premature career termination, alongside substantial financial losses for horse owners. Among the new therapeutic strategies for OA, using mesenchymal stromal cell (MSC)-derived exosomes (MSC-exos) appears to be a promising option for conveying MSC therapeutic potential, yet avoiding the limitations inherent to cell therapy. Here, we first purified and characterized exosomes from MSCs by membrane affinity capture (MAC) and size-exclusion chromatography (SEC). We showed that intact MSC-exos are indeed internalized by equine articular chondrocytes (eACs), and then evaluated their functionality on cartilaginous organoids. Compared to SEC, mRNA and protein expression profiles revealed that MAC-exos induced a greater improvement of eAC-neosynthesized hyaline-like matrix by modulating collagen levels, increasing PCNA, and decreasing Htra1 synthesis. However, because the MAC elution buffer induced unexpected effects on eACs, an ultrafiltration step was included to the isolation protocol. Finally, exosomes from MSCs primed with equine pro-inflammatory cytokines (IL-1β, TNF-α, or IFN-γ) further improved the eAC hyaline-like phenotype, particularly IL-1β and TNF-α. Altogether, these findings indicate the importance of the exosome purification method and further demonstrate the potential of pro-inflammatory priming in the enhancement of the therapeutic value of MSC-exos for equine OA treatment. Full article
(This article belongs to the Special Issue New Discoveries in Cartilage Biology)
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