International Journal of Molecular Sciences

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Medical Applications for 3D Bioprinting 2.0

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Dear Colleagues,

Bioprinting technologies have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix components, and other scaffold-like biomaterials and are currently being used for the manufacture of tissue constructs in tissue engineering and regenerative medicine.

However, many challenging intricacies have to be met before bioprinting technologies achieve their full potential. Novel smart bioinks have to recreate complex tissues and the crosstalk between the different cell phenotypes; in addition, their attributes need to be tailored to the bioprinting modality.

The current Special Issue will cover a selection of articles that inform about novel bioink development, especially for bone, cartilage, tendon/ligament, muscle, and skin. Up-to-date reviews and original papers related to any aspect of medical applications (i.e., creating models for disease research, toxicity screening, functional drug evaluation, or translational clinical research) are welcome.

Dr. Isabel Andia
Prof. Dr. Giovanna Della Porta
Guest Editors

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Keywords

bioprinting
biopolymers
biomaterials
drug delivery
healing mechanisms
signaling proteins
biophysics

Published Papers (2 papers)

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Research

14 pages, 4277 KiB

Open AccessArticle

Release Kinetics and In Vitro Characterization of Sodium Percarbonate and Calcium Peroxide to Oxygenate Bioprinted Tissue Models

by Dongxu Ke, Carlos Kengla, Sang Jin Lee, James J. Yoo, Xuesong Zhu and Sean Vincent Murphy

Int. J. Mol. Sci. 2022, 23(12), 6842; https://doi.org/10.3390/ijms23126842 - 20 Jun 2022

Cited by 7 | Viewed by 1627

Abstract

Oxygen-generating materials have been used in several tissue engineering applications; however, their application as in situ oxygen supply within bioprinted constructs has not been deeply studied. In this study, two oxygen-generating materials, sodium percarbonate (SPO) and calcium peroxide (CPO), were studied for their oxygen release kinetics under a 0.1% O₂ condition. In addition, a novel cell-culture-insert setup was used to evaluate the effects of SPO and CPO on the viability of skeletal muscle cells under the same hypoxic condition. Results showed that SPO had a burst oxygen release, while CPO had a more stable oxygen release than SPO. Both SPO and CPO reduced cell viability when used alone. The addition of catalase in SPO and CPO increased the oxygen release rate, as well as improving the viability of skeletal muscle cells; however, CPO still showed cytotoxicity with catalase. Additionally, the utilization of 1 mg/mL SPO and 20 U catalase in a hydrogel for bioprinting significantly enhanced the cell viability under the hypoxic condition. Moreover, bioprinted muscle constructs could further differentiate into elongated myotubes when transferring back to the normoxic condition. This work provides an excellent in vitro model to test oxygen-generating materials and further discover their applications in bioprinting, where they represent promising avenues to overcome the challenge of oxygen shortage in bioprinted constructs before their complete vascularization. Full article

(This article belongs to the Special Issue Medical Applications for 3D Bioprinting 2.0)

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19 pages, 8580 KiB

Open AccessArticle

Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting

by Cristina Del Amo, Xabier Fernández-San Argimiro, María Cascajo-Castresana, Arantza Perez-Valle, Iratxe Madarieta, Beatriz Olalde and Isabel Andia

Int. J. Mol. Sci. 2022, 23(5), 2836; https://doi.org/10.3390/ijms23052836 - 04 Mar 2022

Cited by 9 | Viewed by 2313

Abstract

In patients with comorbidities, a large number of wounds become chronic, representing an overwhelming economic burden for healthcare systems. Engineering the microenvironment is a paramount trend to activate cells and burst-healing mechanisms. The extrusion bioprinting of advanced dressings was performed with novel composite bioinks made by blending adipose decellularized extracellular matrix with plasma and human dermal fibroblasts. Rheological and microstructural assessments of the composite hydrogels supported post-printing cell viability and proliferation over time. Embedded fibroblasts expressed steady concentrations of extracellular matrix proteins, including type 1, 3 and 4 collagens and fibronectin. ELISA assessments, multiplex protein arrays and ensuing bioinformatic analyses revealed paracrine activities corresponding to wound-healing activation through the modulation of inflammation and angiogenesis. The two modalities of advanced dressings, differing in platelet number, showed differences in the release of inflammatory and angiogenic cytokines, including interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The conditioned media stimulated human-dermal-cell proliferation over time. Our findings open the door to engineering the microenvironment as a strategy to enhance healing. Full article

(This article belongs to the Special Issue Medical Applications for 3D Bioprinting 2.0)

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