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Proteomics and Nanotechnology for Medical and Pharmacological Applications

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pharmacology".

Deadline for manuscript submissions: 20 May 2024 | Viewed by 1201

Special Issue Editor


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Guest Editor
1. Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal
2. UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal
3. LAQV/REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Interests: proteomics; body fluids; peptidome; antimicrobial peptides; bioinformatics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nanotechnologies have made significant advances in the area of medical and pharmacological applications. By using nanomaterials, researchers can develop and produce new drugs, medical devices, diagnostic tools, and treatments that are more precise and effective than ever before. Nanomaterials have enabled researchers to develop nanorobots and nanoparticles that can be used to detect, target, and treat diseases at the cellular level. Nanoparticles are also being used to deliver medications directly to cancer cells, and nanomaterials are being used to create more efficient drug delivery systems. Additionally, nanomaterials are being used to develop new technologies for medical imaging, such as MRI, CT scans, and ultrasounds, to diagnose diseases more accurately. By interacting with biological molecules, i.e., at the nanoscale, nanotechnology opens up a wide field for research and application. This Special Issue contains articles describing recent advances in proteomics to characterize such for medical and pharmacological applications.

Topics of this Special Issue include but are not limited to:

  • Genomic/transcriptomics/proteomics;
  • Peptides and peptidomimetic drugs;
  • Glycopeptides;
  • Imaging and analytical techniques;
  • Peptide-based proteomics databases;
  • New bioinformatics approaches for the integration of proteomic and transcriptomic datasets;
  • Sample preparation for two-dimensional electrophoresis;
  • Sample preparation for protein mass spectrometry;
  • Enrichment for sub-proteomes;
  • Organelle proteomics;
  • Clinical applications of proteomics;
  • Proteomics of non-model organisms;
  • Nanotechnology applications for viral diseases;
  • Materials for antimicrobial applications.

Dr. Rui Vitorino
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomolecules
  • peptides
  • proteomics
  • transcriptomics
  • nanotechnology
  • venomics

Published Papers (1 paper)

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Research

18 pages, 4862 KiB  
Article
Unbiased Phosphoproteome Mining Reveals New Functional Sites of Metabolite-Derived PTMs Involved in MASLD Development
by Eduardo Moltó, Cristina Pintado, Ruy Andrade Louzada, Ernesto Bernal-Mizrachi, Antonio Andrés, Nilda Gallardo and Elena Bonzon-Kulichenko
Int. J. Mol. Sci. 2023, 24(22), 16172; https://doi.org/10.3390/ijms242216172 - 10 Nov 2023
Viewed by 841
Abstract
Post-translational modifications (PTMs) of proteins are paramount in health and disease. Phosphoproteome analysis by enrichment techniques is becoming increasingly attractive for biomedical research. Recent findings show co-enrichment of other phosphate-containing biologically relevant PTMs, but these results were obtained by closed searches focused on [...] Read more.
Post-translational modifications (PTMs) of proteins are paramount in health and disease. Phosphoproteome analysis by enrichment techniques is becoming increasingly attractive for biomedical research. Recent findings show co-enrichment of other phosphate-containing biologically relevant PTMs, but these results were obtained by closed searches focused on the modifications sought. Open searches are a breakthrough in high-throughput PTM analysis (OS-PTM), identifying practically all PTMs detectable by mass spectrometry, even unknown ones, with their modified sites, in a hypothesis-free and deep manner. Here we reanalyze liver phosphoproteome by OS-PTM, demonstrating its extremely complex nature. We found extensive Lys glycerophosphorylations (pgK), as well as modification with glycerylphosphorylethanolamine on Glu (gpetE) and flavin mononucleotide on His (fmnH). The functionality of these metabolite-derived PTMs is demonstrated during metabolic dysfunction-associated steatotic liver disease (MASLD) development in mice. MASLD elicits specific alterations in pgK, epgE and fmnH in the liver, mainly on glycolytic enzymes and mitochondrial proteins, suggesting an increase in glycolysis and mitochondrial ATP production from the early insulin-resistant stages. Thus, we show new possible mechanisms based on metabolite-derived PTMs leading to intrahepatic lipid accumulation during MASLD development and reinforce phosphoproteome enrichment as a valuable tool with which to study the functional implications of a variety of low-abundant phosphate-containing PTMs in cell physiology. Full article
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