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Special Issue "New Research on Strains Improvement and Microbial Biosynthesis"

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A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: 31 January 2024 | Viewed by 1578

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Special Issue Editors

Prof. Dr. Kaloyan Petrov

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Guest Editor

Institute of Chemical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria
Interests: biofuels; process optimization; waste substrates utilization

Prof. Dr. Penka Petrova

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Guest Editor

Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
Interests: genomics; transcriptomics; bioactive peptides

Special Issue Information

Dear Colleagues,

Microbial biosynthesis is the foundation of numerous biotechnological processes for the production of invaluable chemicals widely used as fuels, platform reagents, pharmaceuticals, and various ingredients in food and cosmetics everyday life. Biosynthetic pathways also provide a shortcut to the valorization of renewable natural resources and waste compounds, and a means for the development of a circular economy.

This special issue aims to bring together the latest research in the field of fermentation processes for the biosynthesis of primary and secondary metabolites by genetically improved microbial strains. The scope extends to low molecular weight compounds such as organic acids and alcohols, natural and recombinant enzymes, and biologically active molecules with a protein or lipopeptide nature. Despite the prevailing opinion that the most stable microbial strains applied in industrial biotechnologies are the wild-type isolates found after prolonged selection, modern approaches to genetic engineering offer vast horizons for improvement. In this way, new or enhanced biochemical pathways could be obtained, by-products could be marginalized, and the tolerance to the final biosynthetic product could be increased.

The present special issue provides a forum for cutting-edge research in genomics, mutagenesis, and metabolic/enzyme engineering whose purpose is to achieve enhanced biosynthesis of final metabolites. The development of biotechnologies for novel metabolites or highly optimized microbial processes for the overproduction of those already available, as well as their synthesis from renewable substrates or waste biomass, is also welcomed.

Prof. Dr. Kaloyan Petrov
Prof. Dr. Penka Petrova
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

genetic improvement
biosynthetic pathways engineering
microbial biosynthesis of new metabolites
biofuels
enzymes production
bioactive molecules
process optimization

Published Papers (3 papers)

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Research

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15 pages, 7839 KiB

Open AccessArticle

Whole-Genome Analysis of Novacetimonas cocois and the Effects of Carbon Sources on Synthesis of Bacterial Cellulose

and

Fermentation 2023, 9(11), 972; https://doi.org/10.3390/fermentation9110972 - 14 Nov 2023

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Abstract

Novacetimonas cocois WE7 (formally named Komagataeibacter cocois WE7) is a strain isolated from contaminated coconut milk, capable of producing bacterial cellulose (BC). We sequenced its genome to investigate why WE7 cannot synthesize BC from glucose efficiently. It contains about 3.5 Mb and six plasmid DNAs. N. cocois WE7 contains two bcs operons (bacterial cellulose operon, bcs I and bcs II); the absence of bcs III operons may lead to reduced BC production. From genome predictions, glucose, sucrose, fructose, maltose, and glycerol can be utilized to generate BC, with WE7 unable to metabolize carbohydrate carbon sources through the Embden–Meyerhof–Parnas (EMP) pathway, but rather through the Hexose Monophosphate Pathway (HMP) and tricarboxylic acid (TCA) pathways. It has a complete gluconic acid production pathway, suggesting that BC yield might be very low when glucose, maltose, and trehalose are used as carbon sources. This study represents the first genome analysis of N. cocois. This information is crucial for understanding BC production and regulation mechanisms in N. cocois and lays a foundation for constructing engineered strains tailored for diverse BC application purposes. Full article

(This article belongs to the Special Issue New Research on Strains Improvement and Microbial Biosynthesis)

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15 pages, 6846 KiB

Open AccessArticle

Effects of Aromatic Compounds Degradation on Bacterial Cell Morphology

Maria Gerginova

Gulzhan Spankulova

Tsvetelina Paunova-Krasteva

Nadejda Peneva

Stoyanka Stoitsova

and

Zlatka Alexieva

Fermentation 2023, 9(11), 957; https://doi.org/10.3390/fermentation9110957 - 08 Nov 2023

Viewed by 534

Abstract

The aim of the present study was to evaluate in parallel the capacity of three bacterial strains originating from oil-polluted soils to degrade monoaromatic compounds and the alterations in the bacterial cell morphology as a result of the biodegradation. The strain Gordonia sp. 12/5 can grow well in media containing catechol, o-, m-, and p-cresol without significant morphological changes in the cells, as shown by scanning electron microscopy. This implies good adaptation of the strain for growth in hydrocarbon-containing media and indicates it is a proper candidate strain for further development of purification methodologies applicable to ecosystems contaminated with such compounds. The growth of the two Rhodococcus strains in the presence of the above carbon sources is accompanied by changes in cell size characteristic of stress conditions. Nevertheless, their hydrocarbon-degrading capacity should not be neglected for future applications. In summary, the established ability to degrade monoaromatic compounds, in parallel with the morphological changes of the bacterial cells, can be used as a valuable indicator of the strain’s vitality in the presence of tested aromatic compounds and, accordingly, of its applicability for bioremediation purposes. Full article

(This article belongs to the Special Issue New Research on Strains Improvement and Microbial Biosynthesis)

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Review

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14 pages, 1164 KiB

Open AccessReview

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

and

Fermentation 2023, 9(12), 1000; https://doi.org/10.3390/fermentation9121000 - 25 Nov 2023

Viewed by 331

Abstract

Vitamin C, a water-soluble vitamin with strong reducing power, cannot be synthesized by the human body and participates in a variety of important biochemical reactions. Vitamin C is widely used in the pharmaceutical, food, health care, beverage, cosmetics, and feed industries, with a huge market demand. The classical two-step fermentation method is the mainstream technology for vitamin C production. D-sorbitol is transformed into L-sorbose by Gluconobacter oxydans in the first step of fermentation; then, L-sorbose is transformed into 2-keto-L-gulonic acid (2-KGA) by a coculture system composed of Ketogulonicigenium vulgare and associated bacteria; and finally, 2-KGA is transformed into vitamin C through chemical transformation. The conversion of L-sorbose into 2-KGA in the second fermentation step is performed by K. vulgare. However, considering the slow growth and low 2-KGA production of K. vulgare when cultured alone, it is necessary to add an associated bacteria to stimulate K. vulgare growth and 2-KGA production. Although the mechanism by which the associated bacteria promote K. vulgare growth and 2-KGA production has extensively been studied, this remains a hot topic in related fields. Based on the latest achievements and research, this review summarizes the metabolic characteristics of K. vulgare and associated bacteria and elucidates the mechanism by which the associated bacteria promote the growth and 2-KGA production of K. vulgare. Full article

(This article belongs to the Special Issue New Research on Strains Improvement and Microbial Biosynthesis)

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Special Issue "New Research on Strains Improvement and Microbial Biosynthesis"

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