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Cells
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Mitochondria: New Findings from Single Cells to Organs
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Mitochondria: New Findings from Single Cells to Organs

A special issue of Cells (ISSN 2073-4409).

Deadline for manuscript submissions: closed (15 June 2023) | Viewed by 10860

Special Issue Editors

Dr. Marta Giacomello

E-Mail Website
Guest Editor
1. Department of Biology, University of Padova, 35121 Padova, Italy
2. Department of Biomedical Sciences, University of Padova, Via U. Bassi 58B, 35121 Padova, Italy
Interests: organelles contact sites; high-throughput microscopy; GFP
Special Issues, Collections and Topics in MDPI journals
Dr. Ana Paula Magalhães Rebelo

E-Mail Website
Guest Editor
Department of Biology, University of Padua, Via U. Bassi 58/B, 35121 Padua, Italy
Interests: mitochondria-melanosome contact sites; melanin; mitochondria dynamics
Dr. Martina Semenzato

E-Mail Website
Guest Editor
1. Fondazione Per La Ricerca Biomedica Avanzata, Venetian Institute of Molecular Medicine (VIMM), Via Orus 2, Padova, Italy
2. Department of Biology, University of Padua, Via U. Bassi 58/B, 35121 Padua, Italy
Interests: mitochondrial dynamics; cardiovascular diseases

Special Issue Information

Dear Colleagues,

In recent decades, mitochondria have gained increasing attention because of their plasticity. They are indeed extremely dynamic, able to model the physiology of different areas of the same cell (such as axons, whose biology differs from that of the neuronal body), of whole cells, of various types of cells that communicate among each other, and of whole tissues and organs. Depending on the tissue context, mitochondria can differently shape the cell status or can be shaped themselves. This plasticity also accounts for the number of human pathological phenotypes that are linked to mitochondria defects, which can range from metabolic to neurological dysfunctions to cancer. 

This Special Issue has been raised to highlight the distinct steps of the path that connects individual cells to organ physiology. In this regard, we invite you to contribute reviews and research articles to dissect them, as well as eventual methodological approaches that can help to study mitochondria biology from single cells to whole tissue. 

We look forward to reading your contributions.

Dr. Marta Giacomello
Dr. Ana Paula Magalhães Rebelo
Dr. Martina Semenzato
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mitochondria dynamics
  • metabolism
  • calcium
  • 2D and 3D cultures
  • mitophagy
  • cell death
  • cell signaling

Published Papers (2 papers)

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13 pages, 2303 KiB  
Article
High-Throughput Microscopy Analysis of Mitochondrial Membrane Potential in 2D and 3D Models
by Caterina Vianello, Federica Dal Bello, Sang Hun Shin, Sara Schiavon, Camilla Bean, Ana Paula Magalhães Rebelo, Tomáš Knedlík, Emad Norouzi Esfahani, Veronica Costiniti, Rodrigo S. Lacruz, Giuseppina Covello, Fabio Munari, Tommaso Scolaro, Antonella Viola, Elena Rampazzo, Luca Persano, Sara Zumerle, Luca Scorrano, Alessio Gianelle and Marta Giacomello
Cells 2023, 12(7), 1089; https://doi.org/10.3390/cells12071089 - 05 Apr 2023
Cited by 3 | Viewed by 3843
Abstract
Recent proteomic, metabolomic, and transcriptomic studies have highlighted a connection between changes in mitochondria physiology and cellular pathophysiological mechanisms. Secondary assays to assess the function of these organelles appear fundamental to validate these -omics findings. Although mitochondrial membrane potential is widely recognized as [...] Read more.
Recent proteomic, metabolomic, and transcriptomic studies have highlighted a connection between changes in mitochondria physiology and cellular pathophysiological mechanisms. Secondary assays to assess the function of these organelles appear fundamental to validate these -omics findings. Although mitochondrial membrane potential is widely recognized as an indicator of mitochondrial activity, high-content imaging-based approaches coupled to multiparametric to measure it have not been established yet. In this paper, we describe a methodology for the unbiased high-throughput quantification of mitochondrial membrane potential in vitro, which is suitable for 2D to 3D models. We successfully used our method to analyze mitochondrial membrane potential in monolayers of human fibroblasts, neural stem cells, spheroids, and isolated muscle fibers. Moreover, by combining automated image analysis and machine learning, we were able to discriminate melanoma cells from macrophages in co-culture and to analyze the subpopulations separately. Our data demonstrated that our method is a widely applicable strategy for large-scale profiling of mitochondrial activity. Full article
(This article belongs to the Special Issue Mitochondria: New Findings from Single Cells to Organs)
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Review

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38 pages, 821 KiB  
Review
Mitochondrial Properties in Skeletal Muscle Fiber
by Han Dong and Shih-Yin Tsai
Cells 2023, 12(17), 2183; https://doi.org/10.3390/cells12172183 - 30 Aug 2023
Cited by 7 | Viewed by 6422
Abstract
Mitochondria are the primary source of energy production and are implicated in a wide range of biological processes in most eukaryotic cells. Skeletal muscle heavily relies on mitochondria for energy supplements. In addition to being a powerhouse, mitochondria evoke many functions in skeletal [...] Read more.
Mitochondria are the primary source of energy production and are implicated in a wide range of biological processes in most eukaryotic cells. Skeletal muscle heavily relies on mitochondria for energy supplements. In addition to being a powerhouse, mitochondria evoke many functions in skeletal muscle, including regulating calcium and reactive oxygen species levels. A healthy mitochondria population is necessary for the preservation of skeletal muscle homeostasis, while mitochondria dysregulation is linked to numerous myopathies. In this review, we summarize the recent studies on mitochondria function and quality control in skeletal muscle, focusing mainly on in vivo studies of rodents and human subjects. With an emphasis on the interplay between mitochondrial functions concerning the muscle fiber type-specific phenotypes, we also discuss the effect of aging and exercise on the remodeling of skeletal muscle and mitochondria properties. Full article
(This article belongs to the Special Issue Mitochondria: New Findings from Single Cells to Organs)
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