Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.9
5.4
Journals
Biosensors
Special Issues
Biosensors and Biochips for Cell Analysis
share announcement

Biosensors and Biochips for Cell Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 4636

Special Issue Editor

Dr. Yu-Chih Chen

E-Mail Website
Guest Editor
1. UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
2. Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA
3. CMU-Pitt Ph.D. Program in Computational Biology, Pittsburgh, PA 15213, USA
4. Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15260, USA
Interests: single-cell analysis; cancer precision medicine; microfluidics; machine learning; gene sequencing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Utilizing modern microfabrication technologies, miniaturized biosensors and biochips, which enable precise micro-environment manipulation, minimal reagent usage, and high throughput, have emerged as a state-of-the-art approach for cell biology. While a number of studies have employed microfluidic platforms for functional and molecular cell analysis, the dissemination and standardization of biosensors and biochips are still limited. To advance biosensors and biochips, this Special Issue seeks to collect research papers, short communications, and review articles that focus on (1) the novel biosensors and biochips designs that significantly enhance the throughput, precision, functions of cell analysis, and integration of multi-dimensional cell analysis, (2) single-cell analysis to investigate the functional and molecular cellular heterogeneity, and (3) the methods to enable low-cost and reliable micromanufacturing, high-throughput experimentation and automation for microfluidic cell analysis.

Dr. Yu-Chih Chen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microfluidic cell assays
  • single-cell analysis
  • cell analysis
  • lab on a chip
  • biosensor
  • micrototal analysis systems
  • high-throughput screening
  • automation

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

13 pages, 3607 KiB  
Article
The Influence of Electrode Design on Detecting the Effects of Ferric Ammonium Citrate (FAC) on Pre-Osteoblast through Electrical Cell-Substrate Impedance Sensing (ECIS)
by Zheyuan Zhang, Xichen Yuan, Huijie Guo and Peng Shang
Biosensors 2023, 13(3), 322; https://doi.org/10.3390/bios13030322 - 27 Feb 2023
Cited by 4 | Viewed by 1342
Abstract
Detection sensitivity is a crucial factor in the application of ECIS sensors. For these biosensors, the electrode configuration has a direct impact on sensitivity, yet few studies on monopolar electrodes have been reported. In this study, ECIS sensor arrays, which have a series [...] Read more.
Detection sensitivity is a crucial factor in the application of ECIS sensors. For these biosensors, the electrode configuration has a direct impact on sensitivity, yet few studies on monopolar electrodes have been reported. In this study, ECIS sensor arrays, which have a series of working electrode configuration with a wide diameter range and different electrode number, were fabricated to monitor living osteoblast-like MC3T3-E1 cells. The experimental results revealed that when the electrode diameter was larger than 25 μm, electrodes with smaller diameter and number yielded higher impedance values and generated more impedance shift to cell status change. The membrane capacitance obtained by equivalent circuit fitting was at the same level. When the electrode diameter was even smaller, the results in detection of cell monolayer were opposite, and there was no distinct relationship between impedance and membrane capacitance shift to cell status change and electrode geometry. The proposed sensor chip, allowing for a sustained and stable detection of cellular impedance, provides the basis for the selection of the electrode configuration of monopolar electrodes. The test results of electrodes with a diameter of 25 μm and lower indicated the possibility of single cell impedance measurement, which can provide unique insight into the heterogeneous electrical behavior of cells, and, in this case, the electrode size should be close to the cell size. Full article
(This article belongs to the Special Issue Biosensors and Biochips for Cell Analysis)
►▼ Show Figures

Figure 1

15 pages, 2699 KiB  
Article
Study Hypoxic Response under Cyclic Oxygen Gradients Generated in Microfluidic Devices Using Real-Time Fluorescence Imaging
by Dao-Ming Chang and Yi-Chung Tung
Biosensors 2022, 12(11), 1031; https://doi.org/10.3390/bios12111031 - 17 Nov 2022
Viewed by 1349
Abstract
Oxygen plays important roles in regulating various biological activities under physiological and pathological conditions. However, the response of cells facing temporal variation in oxygen microenvironments has seldom been studied due to technical limitations. In this paper, an integrated approach to studying hypoxic response [...] Read more.
Oxygen plays important roles in regulating various biological activities under physiological and pathological conditions. However, the response of cells facing temporal variation in oxygen microenvironments has seldom been studied due to technical limitations. In this paper, an integrated approach to studying hypoxic response under cyclic oxygen gradients is developed. In the experiments, a cell culture system based on a microfluidic device is constructed to generate cyclic oxygen gradients with desired periods by alternately introducing gases with specific compositions into the microfluidic channels next to the cell culture channel separated by thin channel walls. Observation of the hypoxic responses is performed using real-time fluorescence imaging of dyes sensitive to extra- and intracellular oxygen tensions as well as intracellular calcium concentrations. Cellular hypoxic responses of human aortic smooth muscle cells (AoSMCs) and lung carcinoma epithelium (A549) cells, including intracellular oxygen and calcium levels, are measured. The results show that the two types of cells have different hypoxic responses to the applied cyclic oxygen gradients. With the capability of real-time cellular response monitoring under cyclic oxygen gradients, the developed approach provides a useful scheme to investigate hypoxic responses in vitro under microenvironments mimicking various in vivo physiological and pathological conditions. Full article
(This article belongs to the Special Issue Biosensors and Biochips for Cell Analysis)
►▼ Show Figures

Figure 1

Review

Jump to: Research

25 pages, 9120 KiB  
Review
Molecularly Targeted Fluorescent Sensors for Visualizing and Tracking Cellular Senescence
by Zhirong He, Kun Xu, Yongming Li, Han Gao, Tingting Miao, Rui Zhao and Yanyan Huang
Biosensors 2023, 13(9), 838; https://doi.org/10.3390/bios13090838 - 23 Aug 2023
Cited by 2 | Viewed by 1514
Abstract
Specific identification and monitoring of senescent cells are essential for the in-depth understanding and regulation of senescence-related life processes and diseases. Fluorescent sensors providing real-time and in situ information with spatiotemporal resolution are unparalleled tools and have contributed greatly to this field. This [...] Read more.
Specific identification and monitoring of senescent cells are essential for the in-depth understanding and regulation of senescence-related life processes and diseases. Fluorescent sensors providing real-time and in situ information with spatiotemporal resolution are unparalleled tools and have contributed greatly to this field. This review focuses on the recent progress in fluorescent sensors for molecularly targeted imaging and real-time tracking of cellular senescence. The molecular design, sensing mechanisms, and biological activities of the sensors are discussed. The sensors are categorized by the types of markers and targeting ligands. Accordingly, their molecular recognition and fluorescent performance towards senescence biomarkers are summarized. Finally, the perspective and challenges in this field are discussed, which are expected to assist future design of next-generation sensors for monitoring cellular senescence. Full article
(This article belongs to the Special Issue Biosensors and Biochips for Cell Analysis)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop