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Special Issue "Application of Microfluidics in Cell Manipulation and Biosensing"

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A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 5446

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Special Issue Editors

Dr. Yupan Wu

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Guest Editor

School of Microelectronics, Northwestern Polytechnical University, Xi’an, China
Interests: microfluidics technology; electrokinetic; acoustofluidics; cell manipulation
Special Issues, Collections and Topics in MDPI journals

Dr. Ye Tian

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Guest Editor

College of Medicine and Biological Information Engineering, Northeastern University, Shenyang 110167, China
Interests: microfluidics; sensor and probe; POCT; flexible electroics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, point-of-care applications and high-throughput screening in the healthcare and pharmaceutical industries have resulted in the rapid development of microfluidic systems for cell biology and disease diagnostics. The microfluidic method has attracted significant attention in biological and chemical applications, especially in cell manipulation, mixing, and biosensing, due to its distinctive advantages.

Recent advances in microfluidics, such as the development of point-of-care devices for isolating bioparticles (cancer cells, exosomes, and nanoparticles), microfluidic systems for biosensing and mixing, and cell patterning and culturing using acoustic radiation force and the 3D characterization of cells, address many of the limitations of conventional methods.

In this Special Issue, we would like to invite, but not be limited to, the submission of research on the development of technologies and devices using the microfluidic method for bio/chemical analyses, such as cell manipulation, immunoassay sensors, cell rotating, cell sorting, cell patterning systems, and biosensing. Meanwile, immunoassay sensors and cell manipulation devices using acoustofluidic, electric, magnetic, and optofluidic approaches, as well as the discussion of potential opportunities and challenges of these fields, are also welcome. We encourage you to submit your manuscript with us and publish it.

Dr. Yupan Wu
Dr. Ye Tian
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

microfluidic chip
immunoassays
cell manipulation
biosensing
acoustofluidics

Published Papers (3 papers)

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Research

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13 pages, 3068 KiB

Open AccessArticle

A 3D Capillary-Driven Multi-Micropore Membrane-Based Trigger Valve for Multi-Step Biochemical Reaction

and

Biosensors 2023, 13(1), 26; https://doi.org/10.3390/bios13010026 - 26 Dec 2022

Cited by 1 | Viewed by 1230

Abstract

Point-of-care testing (POCT) techniques based on microfluidic devices enabled rapid and accurate tests on-site, playing an increasingly important role in public health. As the critical component of capillary-driven microfluidic devices for POCT use, the capillary microfluidic valve could schedule multi-step biochemical operations, potentially being used for broader complex POCT tasks. However, owing to the reciprocal relationship between the capillary force and aperture in single-pore microchannels, it was challenging to achieve a high gating threshold and high operable liquid volume simultaneously with existing 2D capillary trigger valves. This paper proposed a 3D capillary-driven multi-microporous membrane-based trigger valve to address the issue. Taking advantage of the high gating threshold determined by micropores and the self-driven capillary channel, a 3D trigger valve composed of a microporous membrane for valving and a wedge-shaped capillary channel for flow pumping was implemented. Utilizing the capillary pinning effect of the multi-micropore membrane, the liquid above the membrane could be triggered by putting the drainage agent into the wedge-shaped capillary channel to wet the underside of the membrane, and it could also be cut off by taking away the agent. After theoretical analysis and performance characterizations, the 3D trigger valve performed a high gating threshold (above 1000 Pa) and high trigger efficiency with an operable liquid volume above 150 μL and a trigger-to-drain time below 6 s. Furthermore, the retention and trigger states of the valve could be switched for repeatable triggering for three cycles within 5 min. Finally, the microbead-based immunoreaction and live cell staining applications verified the valve’s ability to perform multi-step operations. The above results showed that the proposed 3D trigger valve could be expected to play a part in wide-ranging POCT application scenarios. Full article

(This article belongs to the Special Issue Application of Microfluidics in Cell Manipulation and Biosensing)

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16 pages, 6639 KiB

Open AccessArticle

Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method

and

Biosensors 2022, 12(9), 766; https://doi.org/10.3390/bios12090766 - 19 Sep 2022

Cited by 3 | Viewed by 1806

Abstract

Oocyte vitrification technology is widely used for assisted reproduction and fertility preservation, which requires precise washing sequences and timings of cryoprotectant agents (CPAs) treatment to relieve the osmotic shock to cells. The gold standard Cryotop method is extensively used in oocyte vitrification and is currently the most commonly used method in reproductive centers. However, the Cryotop method requires precise and complex manual manipulation by an embryologist, whose proficiency directly determines the effect of vitrification. Therefore, in this study, an automatic microfluidic system consisting of a novel open microfluidic chip and a set of automatic devices was established as a standardized operating protocol to facilitate the conventional manual Cryotop method and minimize the osmotic shock applied to the oocyte. The proposed open microfluidic system could smoothly change the CPA concentration around the oocyte during vitrification pretreatment, and transferred the treated oocyte to the Cryotop with a tiny droplet. The system better conformed to the operating habits of embryologists, whereas the integration of commercialized Cryotop facilitates the subsequent freezing and thawing processes. With standardized operating procedures, our system provides consistent treatment effects for each operation, leading to comparable survival rate, mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) level of oocytes to the manual Cryotop operations. The vitrification platform is the first reported microfluidic system integrating the function of cells transfer from the processing chip, which avoids the risk of cell loss or damage in a manual operation and ensures the sufficient cooling rate during liquid nitrogen (

{LN}_{2}

) freezing. Our study demonstrates significant potential of the automatic microfluidic approach to serve as a facile and universal solution for the vitrification of various precious cells. Full article

(This article belongs to the Special Issue Application of Microfluidics in Cell Manipulation and Biosensing)

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Review

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25 pages, 6351 KiB

Open AccessReview

Photonic Microfluidic Technologies for Phytoplankton Research

José Francisco Algorri

Pablo Roldán-Varona

María Gabriela Fernández-Manteca

José Miguel López-Higuera

Luis Rodriguez-Cobo

and

Adolfo Cobo-García

Biosensors 2022, 12(11), 1024; https://doi.org/10.3390/bios12111024 - 16 Nov 2022

Cited by 1 | Viewed by 1485

Abstract

Phytoplankton is a crucial component for the correct functioning of different ecosystems, climate regulation and carbon reduction. Being at least a quarter of the biomass of the world’s vegetation, they produce approximately 50% of atmospheric O

_{2}

and remove nearly a third of [...] Read more.

_{2}

and remove nearly a third of the anthropogenic carbon released into the atmosphere through photosynthesis. In addition, they support directly or indirectly all the animals of the ocean and freshwater ecosystems, being the base of the food web. The importance of their measurement and identification has increased in the last years, becoming an essential consideration for marine management. The gold standard process used to identify and quantify phytoplankton is manual sample collection and microscopy-based identification, which is a tedious and time-consuming task and requires highly trained professionals. Microfluidic Lab-on-a-Chip technology represents a potential technical solution for environmental monitoring, for example, in situ quantifying toxic phytoplankton. Its main advantages are miniaturisation, portability, reduced reagent/sample consumption and cost reduction. In particular, photonic microfluidic chips that rely on optical sensing have emerged as powerful tools that can be used to identify and analyse phytoplankton with high specificity, sensitivity and throughput. In this review, we focus on recent advances in photonic microfluidic technologies for phytoplankton research. Different optical properties of phytoplankton, fabrication and sensing technologies will be reviewed. To conclude, current challenges and possible future directions will be discussed. Full article

(This article belongs to the Special Issue Application of Microfluidics in Cell Manipulation and Biosensing)

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