Antioxidant Defenses against Pollution and Other Environmental Stresses

A special issue of Antioxidants (ISSN 2076-3921).

Deadline for manuscript submissions: 30 June 2024 | Viewed by 3356

Special Issue Editors


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Guest Editor
Department of Biology, University of Padova, 35122 Padova, Italy
Interests: antioxidant defenses; climate change; molecular evolution; physiological responses to stress
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Department of Biology, University of Padova, Padua, Italy
Interests: physiological responses of animals to environmental conditions where there are chemical contaminants that can also induce oxidative stress
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

All living organisms are, more or less, subject to stressful conditions. In some cases, these conditions depend on their natural ways of life, such as diet or locomotor activity. In other cases, they are linked to environmental conditions, which are modified by both abiotic and biotic factors. In particular, the former includes all the global changes that are affecting our planet, from climatic change to those dependent on anthropogenic activity, such as environmental contamination or acidification of the oceans.

During their evolution, living organisms have developed antioxidant defences, designed to counteract oxidative stress, which are expressed at the systemic, cellular and molecular levels.

This Special Issue aims to examine different aspects of oxidative stress, both in terms of effects on organisms and physiological responses useful to limit or avoid negative effects. Both field and laboratory studies will be considered, targeting organisms from all kingdoms.

Dr. Gianfranco Santovito
Dr. Paola Irato
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

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Published Papers (2 papers)

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14 pages, 2457 KiB  
Article
Drought and Oxidative Stress in Flax (Linum usitatissimum L.) Entails Harnessing Non-Canonical Reference Gene for Precise Quantification of qRT-PCR Gene Expression
by Prasanta K. Dash, Rhitu Rai, Sharat Kumar Pradhan, Sheelavanta Matha Shivaraj, Rupesh Deshmukh, Rohini Sreevathsa and Nagendra K. Singh
Antioxidants 2023, 12(4), 950; https://doi.org/10.3390/antiox12040950 - 18 Apr 2023
Cited by 4 | Viewed by 1292
Abstract
Flax (Linum usitatissimum L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent. Being a cool (Rabi) season crop, it is affected by unprecedented climatic changes such as high temperature, drought, [...] Read more.
Flax (Linum usitatissimum L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent. Being a cool (Rabi) season crop, it is affected by unprecedented climatic changes such as high temperature, drought, and associated oxidative stress that, globally, impede its growth, production, and productivity. To precisely assess the imperative changes that are inflicted by drought and associated oxidative stress, gene expression profiling of predominant drought-responsive genes (AREB, DREB/CBF, and ARR) was carried out by qRT-PCR. Nevertheless, for normalization/quantification of data obtained from qRT-PCR results, a stable reference gene is mandatory. Here, we evaluated a panel of four reference genes (Actin, EF1a, ETIF5A, and UBQ) and assessed their suitability as stable reference genes for the normalization of gene expression data obtained during drought-induced oxidative stress in flax. Taking together, from the canonical expression of the proposed reference genes in three different genotypes, we report that EF1a as a stand-alone and EF1a and ETIF5A in tandem are suitable reference genes to be used for the real-time visualization of cellular impact of drought and oxidative stress on flax. Full article
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Review

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53 pages, 2991 KiB  
Review
Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis
by Natalia N. Rudenko, Daria V. Vetoshkina, Tatiana V. Marenkova and Maria M. Borisova-Mubarakshina
Antioxidants 2023, 12(11), 2014; https://doi.org/10.3390/antiox12112014 - 17 Nov 2023
Cited by 3 | Viewed by 1619
Abstract
Plants are exposed to a variety of abiotic and biotic stresses leading to increased formation of reactive oxygen species (ROS) in plant cells. ROS are capable of oxidizing proteins, pigments, lipids, nucleic acids, and other cell molecules, disrupting their functional activity. During the [...] Read more.
Plants are exposed to a variety of abiotic and biotic stresses leading to increased formation of reactive oxygen species (ROS) in plant cells. ROS are capable of oxidizing proteins, pigments, lipids, nucleic acids, and other cell molecules, disrupting their functional activity. During the process of evolution, numerous antioxidant systems were formed in plants, including antioxidant enzymes and low molecular weight non-enzymatic antioxidants. Antioxidant systems perform neutralization of ROS and therefore prevent oxidative damage of cell components. In the present review, we focus on the biosynthesis of non-enzymatic antioxidants in higher plants cells such as ascorbic acid (vitamin C), glutathione, flavonoids, isoprenoids, carotenoids, tocopherol (vitamin E), ubiquinone, and plastoquinone. Their functioning and their reactivity with respect to individual ROS will be described. This review is also devoted to the modern genetic engineering methods, which are widely used to change the quantitative and qualitative content of the non-enzymatic antioxidants in cultivated plants. These methods allow various plant lines with given properties to be obtained in a rather short time. The most successful approaches for plant transgenesis and plant genome editing for the enhancement of biosynthesis and the content of these antioxidants are discussed. Full article
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