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Advances in Plant Methods: Antioxidant Activity in Plant Extracts

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Dr. Marta Rodríguez-Ruiz

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Guest Editor

Department of Biochemistry and Molecular and Cellular Biology of Plants, Spanish National Research Council, CSIC, Granada, Spain
Interests: fruit and vegetable antioxidants; proteomics; fruit ripening; reactive oxygen; and nitrogen species metabolism; signaling processes; abiotic stress; nitric oxide extract
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Dr. Salvador González Gordo

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Co-Guest Editor

Department of Biochemistry and Molecular and Cellular Biology of Plants, Spanish National Research Council, CSIC, Granada, Spain
Interests: plant antioxidants; nitric oxide; reactive oxygen and nitrogen species; fruit ripening; abiotic/biotic stress; omics

Special Issue Information

Dear Colleagues,

Plants and fruits are a source of food and play an essential role in maintaining a balance against environmental factors including human-induced stressors, which are becoming a crucial problem nowadays. These stressors and changes affect all stages of the plant’s lifecycle, from development to fruit ripening. A growing number of investigations have shown the significance of good extraction and analysis methods, based on innovation and type of plant, to evaluate several issues.

It is a conditional of quality research that it possesses excellent tools of work such as extraction methods appropriate for analysis of factors such as the following:

Abiotic/biotic stress;
Antioxidant metabolism;
Nitro-oxidative stress;

The present Special Issue of Antioxidants aims to provide the most current extraction and analysis methods in plants and fruits, as well as to improve and extend the knowledge of higher plants related to mechanism of action, antioxidant metabolism, and abiotic/biotic stress. This Special Issue is open to different types of manuscripts, including reviews, original research papers, or new methods.

Dr. Marta Rodríguez-Ruiz
Dr. Salvador González Gordo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

extraction methods
antioxidants
ripening
post-translational modification (PTMs)
redox signaling
nitro-oxidative stress
plant development
reactive oxygen species (ROS)
nitrogen oxygen species (RNS)
abiotic stress
biotic stress
nitric oxide
signaling processes
proteomics

Published Papers (2 papers)

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Research

17 pages, 1789 KiB

Open AccessArticle

The Health-Promoting Quality Attributes, Polyphenols, Iridoids and Antioxidant Activity during the Development and Ripening of Cornelian Cherry (Cornus mas L.)

by Dominika Przybylska, Alicja Z. Kucharska, Narcyz Piórecki and Tomasz Sozański

Antioxidants 2024, 13(2), 229; https://doi.org/10.3390/antiox13020229 - 13 Feb 2024

Viewed by 784

Abstract

This study defined the physicochemical attributes, composition, and antioxidant capacity of four Polish cultivars of cornelian cherry (CC) at six stages of development and ripening. A total of 52 metabolites were identified by UPLC-ESI-qTOF-MS/MS and quantified by HPLC-PDA. In general, phenolic acids, hydrolyzable tannins, flavonols, iridoids, antioxidant activity, organic acids, and vitamin C decreased, while anthocyanins, malic acid, sugars, and titratable acidity increased. For the first time, we determined the evolution of the CC chemical properties and the metabolic behavior and quantified the individual compounds, and groups of compounds during ripening, in particular gallotannins, ellagitannins, iridoids, and organic acids. The main novelty of our study is that CC is a valuable resource for utilization at different degrees of maturity. We showed that unripe fruits in particular deserve valorization, as they contained the highest content of total bioactive phytocompounds (5589.1–6779.6 mg/100 g dw)—primarily phenolic acids > iridoids > tannins—and the highest antioxidant capacity. The intermediate stages were the most abundant in vitamin C (341.1–495.6 mg/100 g dw), ellagic acid (5.9–31.6 mg/100 g dw), gallotannins (47.8–331.1 mg/100 g dw), and loganic acid (1393.0–2839.4 mg/100 g dw). The ripe fruits contained less bioactive phytocompounds (1403.7–1974.6 mg/100 g dw)—primarily iridoids > phenolic acids > tannins > anthocyanins—and the lowest antioxidant capacity. On the other hand, ripe fruits showed the highest content of anthocyanins (30.8–143.2 mg/100 g dw), sugars (36.4–78.9 g/100 g dw), malic acid (5.5–12.2 g/100 g dw), and, favorably for the nutritional applications, the highest sugar-to-acids ratio (3.0–6.4). Our work illustrates in detail that quality attributes and the content of health-promoting phytocompounds in CC depend on the ripening stage and on the cultivar. These results advance the scientific knowledge about CC. Our findings can be helpful to select the optimal properties of CC for the development of diverse functional foods and phytopharmaceuticals applied in the prevention of civilization diseases. Full article

(This article belongs to the Special Issue Advances in Plant Methods: Antioxidant Activity in Plant Extracts)

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21 pages, 3459 KiB

Open AccessArticle

Extraction of Bioactive Compounds from Prestonia mollis Leaves and Their Impregnation into Polylactic Acid Using High-Pressure Technologies: Potential for Biomedical Application

by Gabriel Alfonso Burgos-Briones, Lidia Verano-Naranjo, Cristina Cejudo-Bastante, Alex Alberto Dueñas-Rivadeneira, Casimiro Mantell-Serrano and Lourdes Casas-Cardoso

Antioxidants 2023, 12(10), 1864; https://doi.org/10.3390/antiox12101864 - 15 Oct 2023

Viewed by 1219

Abstract

Enhanced solvent extraction (ESE) and pressurized liquid extraction (PLE) have been used for the first time to obtain antioxidant compounds from Prestonia mollis leaves. The effects of pressure (100–250 bar), temperature (55–75 °C) and the composition of the extraction solvent (ethanol, water and hydroalcoholic mixtures) were evaluated according to multilevel factorial designs. PLE provided the largest extraction yields compared to ESE, as well as a greater impact of the operating conditions studied. The highest total phenolic content was obtained when using a hydroalcoholic mixture (CO₂/ethanol/water 50/25/25) through ESE at 100 bar and 75 °C. The antioxidant capacity of this extract is related to higher concentration levels of the identified flavonoids: Quercetin 3-O-xylosyl-rutinoside, Kaempferol 3-(2G-apiosylrobinobioside) and Kaempferol 4′-glucoside 7-rhamnoside. This extract was tested for the supercritical impregnation of polylactic acid (PLA), which is a polymer widely used in the biomedical industry. The influence of pressure (100–400 bar), temperature (35–55 °C), amount of extract (3–6 mL) and impregnation time (1–2 h) have been evaluated. The best results were obtained by impregnating 3 mL of extract at 100 bar and 55 °C for 2 h, achieving 10% inhibition with DPPH methods. The extract presented a potentially suitable impregnation of PLA for biomedical applications. Full article

(This article belongs to the Special Issue Advances in Plant Methods: Antioxidant Activity in Plant Extracts)

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