Application of Chromatography for Point of Care Diagnosis of Noncommunicable Diseases

Topic Information

Dear Colleagues,

The diagnosis of non-communicable diseases typically involves a comprehensive approach that combines medical history, physical examination, laboratory tests, and imaging studies. Biomarker detection has emerged as a valuable diagnostic tool for specific diseases, but precise and rapid quantification of disease-specific biomarkers requires analytical methods that can enable fast sampling and preconcentration from sample matrices. Therefore, solid-phase extraction and micro-extraction techniques are gaining popularity in medical diagnosis due to their unique advantages, including integrated sampling, extraction, and analysis by GC-MS or LC-MS. This Special Issue focuses on the latest discoveries in the field of non-communicable disease diagnosis and aims to bring together clinical and primary researchers to submit interdisciplinary works on cutting-edge techniques, including sample preparation techniques and chromatography methods. The topic covers the latest progress in the development of point-of-care tests, molecular diagnostic tests, animal models for diseases, appropriate dosages and formulations, as well as artificial intelligence and machine learning predictive tools/models for the following diseases (but not limited to): 1. COPD 2. Stroke 3. Alzheimer's disease 4. Liver disease 5. Chronic kidney disease 6. Sickle cell disease/thalassemia 7. Cancer—breast, cervix, oral, and lung 8. Diabetes 9. Cardiovascular disease.

Dr. Chiranjit Ghosh
Dr. Liqin Chen
Prof. Dr. Keshava Balakrishna
Prof. Dr. Chiranjay Mukhopadhyay
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Analytica analytica	-	-	2020	15.6 Days	CHF 1000	Submit
Metabolites metabolites	4.1	5.3	2011	13.2 Days	CHF 2700	Submit
Separations separations	2.6	2.5	2014	13.6 Days	CHF 2600	Submit
Toxins toxins	4.2	7.5	2009	18.4 Days	CHF 2700	Submit
Molecules molecules	4.6	6.7	1996	14.6 Days	CHF 2700	Submit

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All Analytica Metabolites Separations Toxins Molecules

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Open AccessArticle

Screening and Application of DNA Aptamers for Heparin-Binding Protein

by Xi Zhou, Yingying Cao, Xiaocui Huang, Shuqian Qiu, Xinran Xiang, Huimin Niu, Li Chen, Shuiliang Wang, Zhenyu Lin and Shenghang Zhang

Molecules 2024, 29(8), 1717; https://doi.org/10.3390/molecules29081717 - 10 Apr 2024

Viewed by 424

Abstract

Rapid detection of heparin-binding protein (HBP) is essential for timely intervention in sepsis cases. Current detection techniques are usually antibody-based immunological methods, which have certain problems, such as complexity and slow detection, and fall short in meeting the urgency of clinical needs. The [...] Read more.

Rapid detection of heparin-binding protein (HBP) is essential for timely intervention in sepsis cases. Current detection techniques are usually antibody-based immunological methods, which have certain problems, such as complexity and slow detection, and fall short in meeting the urgency of clinical needs. The application of an aptamer can address these concerns well. In this study, HBP-specific DNA aptamers were screened first. Among which, Apt-01, Apt−02, and Apt−13 had a high affinity for HBP, exhibiting impressive K_D values of 3.42, 1.44, and 1.04 nmol/L, respectively. Then, the aptamer of HBP and its partially complementary primer probe were combined to form double-stranded DNA (dsDNA) and synthesize a circular DNA template. The template is complementary to the primer probe, but due to the presence of dsDNA, ExoIII cleaves C2-13 as an RCA primer probe, rendering the template unable to recognize the primer probe and preventing the RCA reaction from proceeding. When the target is present, it competes with the adapter for recognition and releases C2-13, exposing its 3′ end. After initiating the RCA at room temperature and reacting with SYBR GreenII at 37 °C for 20 min, fluorescence changes can be observed and quantitatively analyzed at a 530 nm wavelength, achieving quantitative biological analysis. Apt-01 was used to develop a fluorescent biosensor for HBP detection, which exhibited a good linear range (0.01 nmol/L to 10 nmol/L) and detection limit (0.0056 nmol/L). This advancement holds the potential to lay a solid groundwork for pioneering sensitive and specific methods for HBP detection and to significantly enhance the diagnostic processes for sepsis. Full article

(This article belongs to the Topic Application of Chromatography for Point of Care Diagnosis of Noncommunicable Diseases)

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