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Baculovirus as Vaccine Vectors, RNA Interference Mediators, and Gene Delivery...
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Baculovirus as Vaccine Vectors, RNA Interference Mediators, and Gene Delivery Vectors

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Insect Viruses".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 2252

Special Issue Editor

Prof. Dr. Hung-Jen Liu

E-Mail Website
Guest Editor
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan
Interests: virus–host interaction; signal transduction; cancer virotherapy; vaccine development
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Baculoviruses are DNA viruses which are capable of infecting more than 600 insects. Among these, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the best characterized and most extensively utilized. Baculovirus infects insects in nature and is non-pathogenic to humans. Baculovirus is an attractive and convenient tool for use as a vaccine expression/delivery vector. Baculovirus has been extensively utilized as an excellent tool for producing recombinant proteins in insect cells. In addition to insect cells, baculovirus is capable of transducing a broad range of animal cells. Due to its biosafety, large cloning capacity, low cytotoxicity and non-replication nature in the transduced cells, the ease of manipulation and production, in addition to its protein expression/display, baculovirus has been employed in RNA interference mediators and gene delivery vectors for a wide variety of applications. This Special Issue will highlight the applications of baculoviruses as vaccine vectors for producing vaccines and emphasize the use of baculovirus as RNA interference mediators and gene delivery vectors.

Prof. Dr. Hung-Jen Liu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • baculovirus
  • vaccine vectors
  • subunit vaccine
  • baculovirus display vectors
  • RNA interference mediators
  • gene delivery vectors

Published Papers (2 papers)

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Research

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18 pages, 3765 KiB  
Communication
A Baculovirus Expression Vector Derived Entirely from Non-Templated, Chemically Synthesized DNA Parts
by Christopher Nguyen, Amanda Ibe-Enwo and Jeffrey Slack
Viruses 2023, 15(10), 1981; https://doi.org/10.3390/v15101981 - 23 Sep 2023
Cited by 1 | Viewed by 1276
Abstract
Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput and bespoke classical genome manipulation techniques, we set out to construct a baculovirus vector (>130 kb dsDNA) built from [...] Read more.
Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput and bespoke classical genome manipulation techniques, we set out to construct a baculovirus vector (>130 kb dsDNA) built from modular, chemically synthesized DNA parts. We constructed a synthetic version of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) through two steps of hierarchical Golden Gate assembly. Over 140 restriction endonuclease sites were removed to enable the discrimination of the synthetic genome from native baculovirus genomes. A head-to-head comparison of our modular, synthetic AcMNPV genome with native baculovirus vectors showed no significant difference in baculovirus growth kinetics or recombinant adeno-associated virus production—suggesting that neither baculovirus replication nor very-late gene expression were compromised by our design or assembly method. With unprecedented control over the AcMNPV genome at the single-nucleotide level, we hope to ambitiously explore novel AcMNPV vectors streamlined for biologics production and development. Full article
(This article belongs to the Special Issue Baculovirus as Vaccine Vectors, RNA Interference Mediators, and Gene Delivery Vectors)
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Review

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14 pages, 2846 KiB  
Review
Multi-Gene Recombinant Baculovirus Expression Systems: From Inception to Contemporary Applications
by Sara L. Bissett and Polly Roy
Viruses 2024, 16(4), 492; https://doi.org/10.3390/v16040492 - 23 Mar 2024
Viewed by 643
Abstract
Many protein expression systems are primarily utilised to produce a single, specific recombinant protein. In contrast, most biological processes such as virus assembly rely upon a complex of several interacting proteins rather than the activity of a sole protein. The high complexity of [...] Read more.
Many protein expression systems are primarily utilised to produce a single, specific recombinant protein. In contrast, most biological processes such as virus assembly rely upon a complex of several interacting proteins rather than the activity of a sole protein. The high complexity of the baculovirus genome, coupled with a multiphase replication cycle incorporating distinct transcriptional steps, made it the ideal system to manipulate for high-level expression of a single, or co-expression of multiple, foreign proteins within a single cell. We have developed and utilised a series of recombinant baculovirus systems to unravel the sequential assembly process of a complex non-enveloped model virus, bluetongue virus (BTV). The high protein yields expressed by the baculovirus system not only facilitated structure–function analysis of each viral protein but were also advantageous to crystallography studies and supported the first atomic-level resolution of a recombinant viral protein, the major BTV capsid protein. Further, the formation of recombinant double-shelled virus-like particles (VLPs) provided insights into the structure–function relationships among the four major structural proteins of the BTV whilst also representing a potential candidate for a viral vaccine. The baculovirus multi-gene expression system facilitated the study of structurally complex viruses (both non-enveloped and enveloped viruses) and heralded a new generation of viral vaccines. Full article
(This article belongs to the Special Issue Baculovirus as Vaccine Vectors, RNA Interference Mediators, and Gene Delivery Vectors)
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