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Surface Plasmon Resonance Biosensors for Medical Diagnosis
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Surface Plasmon Resonance Biosensors for Medical Diagnosis

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (31 October 2021) | Viewed by 13144

Special Issue Editor

Prof. Dr. Ewa Gorodkiewicz

E-Mail Website
Guest Editor
Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland
Interests: biosensors; surface plasmon resonance; immunosensors; liquid biopsy; biomarkers; cancer markers
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Medical diagnosis requires new biomarkers and better methods for the determination of the markers already used. Surface plasmon resonance (SPR) techniques offer huge potential in this area, a potential which has so far been exploited only to a marginal degree. The aim of this issue is to serve as a platform bringing together researchers working on the boundary between SPR and medical diagnostic. Bodily fluids such as blood, urine, saliva, and cerebrospinal fluid contain an enormous stream of information, which would be evaluable provided that we knew a set of biomarkers and suitable methods for their determination. The diagnosis of various kinds of cancer, cardiovascular and neurodegenerative diseases, and viral infections would then be much easier, including the determination of the stage of the disease and the effectiveness of medical treatment. SPR techniques offer label-free determination, basically simple biosensor construction, and direct biosensor determination without preliminary marker isolation. The techniques seem to be ideal tools in the search for new biomarkers. The techniques may also be better alternatives to existing methods. Articles may include but are not limited to the following topics:

  • Successful diagnostic applications of SPR
  • SPR for personalized diagnosis
  • Biomarker detection by localized SPR
  • SPR signal enhancement for biomarker determination
  • Cancer detection by SPR biosensors
  • SPR biosensors for neurodegenerative marker detection
  • Virus detection by SPR biosensors
  • Markers for cardiovascular disease determination by SPR
  • New biomarkers discovered with SPR
  • SPR vs. related techniques (SARS, quartz microbalance, interferometry) in diagnosis

Prof. Dr. Ewa Gorodkiewicz
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Liquid biopsy
  • SPR Biosensors
  • SPR Immunosensors
  • Biomarkers by SPR
  • Molecular cancer markers
  • Virus detection
  • SPR for personalized diagnostic
  • Localized SPR
  • SPR signal enhancement

Published Papers (4 papers)

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Research

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15 pages, 5509 KiB  
Article
Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
by Piotr Mrozek, Ewa Gorodkiewicz, Paweł Falkowski and Bogusław Hościło
Sensors 2021, 21(13), 4348; https://doi.org/10.3390/s21134348 - 25 Jun 2021
Cited by 11 | Viewed by 2133
Abstract
Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified [...] Read more.
Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance Biosensors for Medical Diagnosis)
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9 pages, 2071 KiB  
Communication
An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma
by Beata Szymanska, Zenon Lukaszewski, Beata Zelazowska-Rutkowska, Kinga Hermanowicz-Szamatowicz and Ewa Gorodkiewicz
Sensors 2021, 21(10), 3567; https://doi.org/10.3390/s21103567 - 20 May 2021
Cited by 14 | Viewed by 2882
Abstract
Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of [...] Read more.
Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of rabbit polyclonal antibody against HE4, covalently attached to a gold chip via cysteamine linker. The biosensor is used with the non-fluidic array SPRi technique. The linear range of the analytical signal response was found to be 2–120 pM, and the biosensor can be used for the determination of the HE4 marker in the plasma of both healthy subjects and ovarian cancer patients after suitable dilution with a PBS buffer. Precision (6–10%) and recovery (101.8–103.5%) were found to be acceptable, and the LOD was equal to 2 pM. The biosensor was validated by the parallel determination of a series of plasma samples from ovarian cancer patients using the Elecsys HE4 test and the developed biosensor, with a good agreement of the results (a Pearson coefficient of 0.989). An example of the diagnostic application of the developed biosensor is given—the influence of ovarian tumor resection on the level of HE4 in blood serum. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance Biosensors for Medical Diagnosis)
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15 pages, 2629 KiB  
Article
A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent
by Laura Pasquardini, Nunzio Cennamo, Giuseppe Malleo, Lia Vanzetti, Luigi Zeni, Deborah Bonamini, Roberto Salvia, Claudio Bassi and Alessandra Maria Bossi
Sensors 2021, 21(10), 3443; https://doi.org/10.3390/s21103443 - 15 May 2021
Cited by 16 | Viewed by 2930
Abstract
Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed [...] Read more.
Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8–25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance Biosensors for Medical Diagnosis)
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Review

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35 pages, 7081 KiB  
Review
Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics
by Marcin Drozd, Sylwia Karoń and Elżbieta Malinowska
Sensors 2021, 21(11), 3781; https://doi.org/10.3390/s21113781 - 29 May 2021
Cited by 16 | Viewed by 4170
Abstract
The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires [...] Read more.
The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires methods characterized by a very good sensitivity and selectivity. In the case of the SPR technique, as well as other surface-sensitive detection strategies, the quality of the transducer-immunoreceptor interphase is crucial for maintaining the analytical reliability of an assay. In this work, an overview of general approaches to the design of functional SPR-immunoassays is presented. It covers both immunosensors, the design of which utilizes well-known and often commercially available substrates, as well as the latest solutions developed in-house. Various approaches employing chemical and passive binding, affinity-based antibody immobilization, and the introduction of nanomaterial-based surfaces are discussed. The essence of their influence on the improvement of the main analytical parameters of a given immunosensor is explained. Particular attention is paid to solutions compatible with the latest trends in the development of label-free immunosensors, such as platforms dedicated to real-time monitoring in a quasi-continuous mode, the use of in situ-generated receptor layers (elimination of the regeneration step), and biosensors using recombinant and labelled protein receptors. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance Biosensors for Medical Diagnosis)
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