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Surface Plasmon Resonance-Based Biosensor
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Surface Plasmon Resonance-Based Biosensor

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (15 February 2024) | Viewed by 4320

Special Issue Editor

Prof. Dr. Ewa Gorodkiewicz

E-Mail Website
Guest Editor
Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland
Interests: biosensors; surface plasmon resonance; immunosensors; liquid biopsy; biomarkers; cancer markers
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Surface plasmon resonance (SPR) techniques offer huge potential in the determination of biomarkers.

These techniques offer label-free determination, simple biosensor construction, and direct biosensor determination. Different SPR techniques are used: fluidic SPR, SPR imaging, and localised SPR, among others. Biosensors are among the most promising tools used for biomarker determination. Molecular biomarkers, exosomes, and even cancer cells are targets of SPR biosensors. In order to achieve the necessary quantification limit, methods for signal enhancement are used, including sandwich structures with gold nanoparticles or application of quantum dots . SPR techniques are also useful in the search for new biomarkers and can be better alternatives to existing methods.

This Special Issue aims to serve as a platform bringing together researchers working on biosensors based on surface plasmon resonance techniques. Articles may include, but are not limited to, the following topics:

  • New biomarkers discovered with SPR
  • Successful diagnostic applications of SPR
  • SPR for personalized diagnosis
  • Biomarker detection by localized SPR
  • SPR signal enhancement for biomarker determination
  • SPR vs. related techniques (e.g., SERS, quartz microbalance, interferometry) in diagnosis

Prof. Dr. Ewa Gorodkiewicz
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluidic SPR
  • localized SPR
  • SPR signal enhancement
  • SPR for personalized diagnosis
  • SPR biosensors
  • SPR immunosensors
  • biomarkers by SPR
  • molecular cancer markers
  • virus detection
  • liquid biopsy

Published Papers (4 papers)

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Research

12 pages, 2062 KiB  
Article
Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements
by Piotr Mrozek, Lukasz Oldak and Ewa Gorodkiewicz
Sensors 2023, 23(24), 9899; https://doi.org/10.3390/s23249899 - 18 Dec 2023
Viewed by 601
Abstract
Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the [...] Read more.
Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the ligand at increasing analyte concentrations ranging from 0.0 μg mL−1 to 5.0 μg mL−1 in the non-fluidic and the fluidic variant showed that the thickness of the bound anti-mouse antibody layers in the fluidic variant was approximately 1.5–3 times larger than in the non-fluidic variant. The greater thicknesses of the deposited layers were also reflected in the larger increment of the resonant angle in the fluidic variant compared to the non-fluidic variant in the considered range of analyte concentrations. The choice between fluidic and non-fluidic surface plasmon resonance biosensors may be justified by the availability of analyte volume and the intended modulation technique. When working with limited analyte, non-fluidic biosensors with intensity modulation are more advantageous. For larger analyte quantities, fluidic biosensors with angular modulation are recommended, primarily due to their slightly higher sensitivity in this measurement mode. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance-Based Biosensor)
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13 pages, 2706 KiB  
Article
Strategies for Mitigating Commercial Sensor Chip Variability with Experimental Design Controls
by Eliza K. Hanson, Chien-Wei Wang, Lisa Minkoff and Rebecca J. Whelan
Sensors 2023, 23(15), 6703; https://doi.org/10.3390/s23156703 - 26 Jul 2023
Cited by 1 | Viewed by 1096
Abstract
Surface plasmon resonance (SPR) is a popular real-time technique for the measurement of binding affinity and kinetics, and bench-top instruments combine affordability and ease of use with other benefits of the technique. Biomolecular ligands labeled with the 6xHis tag can be immobilized onto [...] Read more.
Surface plasmon resonance (SPR) is a popular real-time technique for the measurement of binding affinity and kinetics, and bench-top instruments combine affordability and ease of use with other benefits of the technique. Biomolecular ligands labeled with the 6xHis tag can be immobilized onto sensing surfaces presenting the Ni2+-nitrilotriacetic acid (NTA) functional group. While Ni-NTA immobilization offers many advantages, including the ability to regenerate and reuse the sensors, its use can lead to signal variability between experimental replicates. We report here a study of factors contributing to this variability using the Nicoya OpenSPR as a model system and suggest ways to control for those factors, increasing the reproducibility and rigor of the data. Our model ligand/analyte pairs were two ovarian cancer biomarker proteins (MUC16 and HE4) and their corresponding monoclonal antibodies. We observed a broad range of non-specific binding across multiple NTA chips. Experiments run on the same chips had more consistent results in ligand immobilization and analyte binding than experiments run on different chips. Further assessment showed that different chips demonstrated different maximum immobilizations for the same concentration of injected protein. We also show a variety of relationships between ligand immobilization level and analyte response, which we attribute to steric crowding at high ligand concentrations. Using this calibration to inform experimental design, researchers can choose protein concentrations for immobilization corresponding to the linear range of analyte response. We are the first to demonstrate calibration and normalization as a strategy to increase reproducibility and data quality of these chips. Our study assesses a variety of factors affecting chip variability, addressing a gap in knowledge about commercially available sensor chips. Controlling for these factors in the process of experimental design will minimize variability in analyte signal when using these important sensing platforms. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance-Based Biosensor)
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12 pages, 2165 KiB  
Article
Sensitivity-Tunable Terahertz Liquid/Gas Biosensor Based on Surface Plasmon Resonance with Dirac Semimetal
by Mengjiao Ren, Chengpeng Ji, Xueyan Tang, Haishan Tian, Leyong Jiang, Xiaoyu Dai, Xinghua Wu and Yuanjiang Xiang
Sensors 2023, 23(12), 5520; https://doi.org/10.3390/s23125520 - 12 Jun 2023
Cited by 1 | Viewed by 843
Abstract
In this paper, we study the sensitivity-tunable terahertz (THz) liquid/gas biosensor in a coupling prism–three-dimensional Dirac semimetal (3D DSM) multilayer structure. The high sensitivity of the biosensor originates from the sharp reflected peak caused by surface plasmon resonance (SPR) mode. This structure achieves [...] Read more.
In this paper, we study the sensitivity-tunable terahertz (THz) liquid/gas biosensor in a coupling prism–three-dimensional Dirac semimetal (3D DSM) multilayer structure. The high sensitivity of the biosensor originates from the sharp reflected peak caused by surface plasmon resonance (SPR) mode. This structure achieves the tunability of sensitivity due to the fact that the reflectance could be modulated by the Fermi energy of 3D DSM. Besides, it is found that the sensitivity curve depends heavily on the structural parameters of 3D DSM. After parameter optimization, we obtained sensitivity over 100°/RIU for liquid biosensor. We believe this simple structure provides a reference idea for realizing high sensitivity and a tunable biosensor device. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance-Based Biosensor)
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10 pages, 2934 KiB  
Article
SPECTRA: A Novel Compact System for Surface Plasmon Resonance Measurements
by Elisabetta Pasqualotto, Erica Cretaio, Lara Franchin, Alessandro De Toni, Alessandro Paccagnella, Stefano Bonaldo and Matteo Scaramuzza
Sensors 2023, 23(9), 4309; https://doi.org/10.3390/s23094309 - 26 Apr 2023
Cited by 1 | Viewed by 1382
Abstract
Surface plasmon resonance (SPR) is a common and useful measurement technique to perform fast and sensitive optical detection. SPR instrumentations usually comprise optical systems of mirrors and lenses which are quite expensive and impractical for point-of-care applications. In this work, we presented a [...] Read more.
Surface plasmon resonance (SPR) is a common and useful measurement technique to perform fast and sensitive optical detection. SPR instrumentations usually comprise optical systems of mirrors and lenses which are quite expensive and impractical for point-of-care applications. In this work, we presented a novel and compact SPR device called SPECTRA, designed as a spectrophotometer add-on with a grating coupling configuration. The device is conceived as a marketable solution to perform quick SPR measurements in grating configuration without the requirement of complex instrumentation. The device can be customized either in a vertical structure to reach lower incident light angles, or in a horizontal configuration, which is suitable for SPR analysis using liquid solutions. The SPECTRA performance was evaluated through SPR measurements in typical applications. The vertical SPECTRA system was employed to detect different functionalization molecules on gold 720 nm-period grating devices. Meanwhile, the horizontal SPECTRA configuration was exploited to carry out fluid-dynamic measurements using a microfluidic cell with glycerol solutions at increasing concentrations to account for different refractive indexes. The experimental tests confirmed that the SPECTRA design is suitable for SPR measurements, demonstrating its capability to detect the presence of analytes and changes in surface properties both in static and dynamic set-ups. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance-Based Biosensor)
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