Advances in Studies on Citrus Antioxidant Compounds Using Traditional and New Biotechnological Approaches

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 3691

Special Issue Editor


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Guest Editor
Research Center for Olive Fruit and Citrus Crops, Council for Agricultural Research and Economics, 95024 Acireale, Italy
Interests: citrus genetic, genomic, transcriptomic, and advanced breeding technology; addressed to the improvement of fruit quality; to the exploitation of resistance to biotic stresses and to the valorization of true-to-typeness of clonal fingerprinting
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Special Issue Information

Dear Colleagues,

Citrus is one of the most economically noteworthy and widely cultivated fruit crops worldwide. Several citrus and related genera are rich in nutraceutical and antioxidant compounds, such as flavonoids, carotenoids, and vitamin C. Most of them are characterized by beautiful colors that are attractive for pollinators; they protect flowers and fruits from UV damage and pathogens. They are also protective against several human diseases. Its interaction with external factors is known to control the biosynthesis and regulation of secondary metabolites. Owing to genetic and epigenetic control, the study on citrus compounds is very complex. Moreover, it is also known that biotic and abiotic stress can modulate and alter the quality and quantity of secondary metabolites. The narrow genetic diversity makes citrus and related ancient species exposed to several diseases (such as citrus canker, Alternaria brown spot, citrus black spot, and Huanglongbing) which are spreading rapidly in most of the world’s citrus-producing countries.

Many efforts have been made by breeders to study and manage the main genetic traits through traditional breeding, with the aim to anticipate the maturity period, produce varieties and hybrids enriched in nutraceutical and antioxidant compounds, as well as tolerant or resistant to main biotic and abiotic threats. These studies are addressed to protect citrus species and its fruits and products. Unfortunately, the long juvenile phase is a real limit to observe the traits of fruits for functional studies. However, much more has to be studied on the biosynthetic and regulatory mechanisms controlling antioxidant and nutraceutical compounds, making fruits and other tissues of citrus and related genera so important and appealing. Ancient citrus represents also a large source of genes and such knowledge needs to be deeply exploited. The study on the genetic diversity underlying the biosynthesis and regulation pathways aims to investigate citrus variability contributing to depict new improved varieties.

Therefore, in this Special Issue of Plants, novel scientific contributions (original research papers, opinions, reviews, perspectives, hypotheses, and new methods) are welcome, focusing on gene expression analysis and related functional studies, application of traditional breeding, and recent molecular and biotechnological advances based on new plant breeding technologies, genetic transformation, and epigenetic studies. All contributions must be focused on creating advancements in citrus crop improvement mainly of traits of fruit as they are naturally, or as consequence of the effect of external factors.

This Special Issue focuses on the following topics:

(i) gene expression analysis and related functional studies
(ii) traditional and molecular breeding
(iii) cisgenesis and genome editing
(iv) genetic transformation
(v) epigenetic studies
(vi) antioxidant and nutraceutical compounds

Dr. Concetta Licciardello
Guest Editor

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Keywords

  • flavonoids
  • carotenoids
  • vitamin C
  • secondary metabolites
  • human health
  • biotic and abiotic stress
  • gene expression
  • plant biotechnology
  • molecular breeding
  • cisgenesis
  • genome editing
  • epigenetics

Published Papers (1 paper)

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Research

15 pages, 1148 KiB  
Article
Genome-Wide Association Study of Healthful Flavonoids among Diverse Mandarin Accessions
by Matthew R. Mattia, Dongliang Du, Qibin Yu, Tracy Kahn, Mikeal Roose, Yoko Hiraoka, Yu Wang, Patricio Munoz and Fred G. Gmitter, Jr.
Plants 2022, 11(3), 317; https://doi.org/10.3390/plants11030317 - 25 Jan 2022
Cited by 5 | Viewed by 2761
Abstract
Mandarins have many unique flavonoids with documented health benefits and that help to prevent chronic human diseases. Flavonoids are difficult to measure and cannot be phenotyped without the use of specialized equipment; consequently, citrus breeders have not used flavonoid contents as selection criteria [...] Read more.
Mandarins have many unique flavonoids with documented health benefits and that help to prevent chronic human diseases. Flavonoids are difficult to measure and cannot be phenotyped without the use of specialized equipment; consequently, citrus breeders have not used flavonoid contents as selection criteria to develop cultivars with increased benefits for human health or increased tolerance to diseases. In this study, peel, pulp, and seed samples collected from many mandarin accessions and their hybrids were analyzed for the presence of selected flavonoids with documented human health benefits. A genome-wide association study (GWAS) was used to identify SNPs associated with biosynthesis of flavonoids in these mandarin accessions, and there were 420 significant SNPs were found to be associated with 28 compounds in peel, pulp, or seed samples. Four candidate genes involved in flavonoid biosynthesis were identified by enrichment analysis. SNPs that were found to be associated with compounds in pulp samples have the potential to be used as markers to select mandarins with improved phytonutrient content to benefit human health. Mandarin cultivars bred with increased flavonoid content may provide value to growers and consumers. Full article
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