Callus Tissue – a New Look on the Proliferation and Regeneration Ability

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: closed (22 December 2022) | Viewed by 3302

Special Issue Editors


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Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 28 Jagiellonska St, 40-032 Katowice, Poland
Interests: cell differentiation; cell wall; auxin; somatic embryogenesis; nanoparticles; symplasmic communication
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Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Kraków, Poland
Interests: plant tissue culture; experimental plant embryology; morphogenesis; cell wall remodeling; cell differentiation

Special Issue Information

Dear Colleagues,

Plant callus tissue is mostly composed of uniform cells, and its presence is observed after wounding or organ loss in natural conditions. However, the abundance of this unorganized tissue is especially noticed under in vitro culture. Although callus tissue has been the subject of many studies for almost a century, there are still open questions about callus induction, proliferation, and finally indirect morphogenesis. Studies on the ability to form de novo organs or regenerate an entire plant from unorganized tissue or even one cell from among the crowd of others within callus are part of our knowledge regarding plant toti- and pluripotency. In addition, the study of reprogramming due to culture conditions also has practical implications: e.g., efficient micropropagation protocols often demand the stage of morphogenic callus, and this tissue can be an important source of second metabolites.

This Special Issue of Plants will highlight all the phenomena related to callus proliferation, differentiation, and plant regeneration. Other novel applications of callus tissue and descriptions of newly discovered regulatory compounds which improve callus induction and proliferation will also be appreciated.

Prof. Dr. Ewa Kurczyńska
Dr. Marzena Popielarska-Konieczna
Guest Editors

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Keywords

  • proliferation
  • differentiation
  • morphogenesis
  • plant growth regulators
  • second metabolites
  • histology
  • ultrastructure
  • cell wall
  • gene expression

Published Papers (1 paper)

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Research

15 pages, 7056 KiB  
Article
Totipotency of Daucus carota L. Somatic Cells Microencapsulated Using Spray Drying Technology
by José Alfredo Santiz-Gómez, Miguel Abud-Archila, Víctor Manuel Ruíz-Valdiviezo, Yazmin Sánchez-Roque and Federico Antonio Gutiérrez-Miceli
Plants 2021, 10(11), 2491; https://doi.org/10.3390/plants10112491 - 18 Nov 2021
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Abstract
The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this [...] Read more.
The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to evaluate the effect of spray drying on the viability and totipotency of somatic carrot cells. Leaf, root and stem explants were evaluated to induce callus with 2 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Calli obtained from the stem were cultivated in a liquid medium with 1 mg/L of 2,4-D. Cell suspensions were spray dried with maltodextrin-gum Arabic and maltodextrin-xanthan gum mixtures, two outlet air temperatures (50 and 60 °C) and 120 °C inlet air temperature. Results showed that carrot cells were viable after spray drying, and this viability remained for six months at 8 °C. The totipotency of the microencapsulated cells was proven. Cells that were not spray dried regenerated 24.6 plantlets, while the spray dried cells regenerated 19 plantlets for each gram of rehydrated powder. Thus, spray drying allowed researchers to obtain viable and totipotent cells. This work is the first manuscript that reported the spray drying of plant somatic cells. Full article
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