Emerging Viruses: Epidemiology and Surveillance Challenge

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Emerging Pathogens".

Deadline for manuscript submissions: 20 June 2024 | Viewed by 538

Special Issue Editor


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Guest Editor
1. Department of Environmental Health Sciences, Tulane University, 1440 Canal Street, New Orleans, LA 70112, USA
2. BioEnvironmental Science Program, Morgan State University, Baltimore, MD 21251, USA
Interests: wastewater based epidemiology; climate change; infectious diseases
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Special Issue Information

Dear Colleagues,

The wastewater surveillance of SARS-CoV-2 is a hot topic, and it can be used by public health officials as an early warning tool. This promising tool can also be expanded for other emerging viruses and pathogens.

Detecting these viruses in wastewater is an ongoing challenge. Multiple concentration and molecular methods have been deployed for the detection of respiratory and enteric viruses, including RT-qPCR, RT-ddPCR, and next-generation sequencing.

To address these needs for monitoring emerging viruses and other pathogens, we would like to invite research articles or review papers focused on various aspects of this field, including, but not limited to, the following:

  • Rapid detection and identification methods for emerging respiratory and enteric viruses;
  • Next-generation-sequencing-based methods;
  • Tracking emerging viruses in wastewater in LMICs.

Dr. Samendra Sherchan
Guest Editor

Manuscript Submission Information

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Keywords

  • wastewater surveillance
  • emerging viruses
  • epidemiology
  • clinical surveillance

Published Papers (1 paper)

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Research

6 pages, 202 KiB  
Communication
Application of Skimmed-Milk Flocculation Method for Wastewater Surveillance of COVID-19 in Kathmandu, Nepal
by Sarmila Tandukar, Ocean Thakali, Ananda Tiwari, Rakshya Baral, Bikash Malla, Eiji Haramoto, Jivan Shakya, Reshma Tuladhar, Dev Raj Joshi, Bhawana Sharma, Bhushan Raj Shrestha and Samendra P. Sherchan
Pathogens 2024, 13(5), 366; https://doi.org/10.3390/pathogens13050366 - 29 Apr 2024
Viewed by 413
Abstract
Wastewater surveillance (WS) has been used globally as a complementary tool to monitor the spread of coronavirus disease 2019 (COVID-19) throughout the pandemic. However, a concern about the appropriateness of WS in low- and middle-income countries (LMICs) exists due to low sewer coverage [...] Read more.
Wastewater surveillance (WS) has been used globally as a complementary tool to monitor the spread of coronavirus disease 2019 (COVID-19) throughout the pandemic. However, a concern about the appropriateness of WS in low- and middle-income countries (LMICs) exists due to low sewer coverage and expensive viral concentration methods. In this study, influent wastewater samples (n = 63) collected from two wastewater treatment plants (WWTPs) of the Kathmandu Valley between March 2021 and February 2022 were concentrated using the economical skimmed-milk flocculation method (SMFM). The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was tested by qPCR using assays that target the nucleocapsid (N) and envelope (E) genes. Overall, 84% (53/63) of the total samples were positive for SARS-CoV-2 according to at least one of the tested assays, with concentrations ranging from 3.5 to 8.3 log10 gene copies/L, indicating the effectiveness of the SMFM. No correlation was observed between the total number of COVID-19 cases and SARS-CoV-2 RNA concentrations in wastewater collected from the two WWTPs (p > 0.05). This finding cautions the prediction of future COVID-19 waves and the estimation of the number of COVID-19 cases based on wastewater concentration in settings with low sewer coverage by WWTPs. Future studies on WS in LMICs are recommended to be conducted by downscaling to sewer drainage, targeting a limited number of houses. Overall, this study supports the notion that SMFM can be an excellent economical virus-concentrating method for WS of COVID-19 in LMICs. Full article
(This article belongs to the Special Issue Emerging Viruses: Epidemiology and Surveillance Challenge)
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