Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.1
3.7
Journals
Pathogens
Special Issues
Advances in HPV Diagnostic Testing—Improving Access and Accuracy
share announcement

Advances in HPV Diagnostic Testing—Improving Access and Accuracy

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Viral Pathogens".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 1338

Special Issue Editor

Dr. Collette Bromhead

E-Mail Website
Guest Editor
School of Health Sciences, Massey University, Auckland 0632, New Zealand
Interests: molecular diagnostics; infectious disease; sexually transmitted infections; viral haemorrhagic fevers; point of care diagnostics; human papillomavirus; cervical cancer screening; capacity building; antimicrobial stewardship
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Oncogenic types of HPV were first identified as the cause of cervical cancer in the 1980s. This lead to the development of highly effective prophylactic HPV vaccines and the eventual implementation of primary screening programmes based on viral nucleic acid detection and partial typing. Diagnostic methods for HPV have evolved considerably in recent years, with the development of more sensitive, specific and/or accessible tests. Methods including extended genotyping, analysis of methylation signatures, viral loads, and other proteomic and bioinformatic approaches continue to be reported.  However, various challenges remain.

There is yet to be an ideal test or algorithm that optimally reduces both the risk of missing relevant disease and over-treatment. Whilst HPV primary cervical cancer screening is well developed, the use and interpretation of those same HPV tests in anal cancer screening or oropharyngeal disease is still being investigated. Additionally, in resource-limited settings, a variety of molecular diagnostic tools have been developed for point of care testing (POCT), but their dependence on expensive equipment, a reliable power supply and other technical requirements limit their applications. Early detection of HPV infection and any resulting abnormalities remains essential for the effective management of HPV-related diseases.

This Special Issue aims to present the latest research on all aspects of HPV diagnostics, from improving the accuracy of disease prediction and detection through to improvements in reliability and acceptability of access and achieving timely testing.  We invite you to share research that contributes new knowledge to this field and that will aid in reducing the morbidity and mortality associated with this globally important virus.

Dr. Collette Bromhead
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • human papillomavirus
  • molecular diagnostics
  • self-testing
  • point of care testing
  • biomarkers
  • cervical intraepithelial neoplasia (CIN)
  • anal intraepithelial neoplasia (AIN)
  • oropharyngeal cancer
  • primary screening
  • methylation
  • viral load
  • mass spectrometry
  • CRISPR

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

12 pages, 762 KiB  
Article
Preliminary Results of Feasibility and Acceptability of Self-Collection for Cervical Screening in Italian Women
by Illari Sechi, Narcisa Muresu, Mariangela V. Puci, Laura Saderi, Arcadia Del Rio, Andrea Cossu, Maria R. Muroni, Santina Castriciano, Marianna Martinelli, Clementina E. Cocuzza, Giovanni Sotgiu and Andrea Piana
Pathogens 2023, 12(9), 1169; https://doi.org/10.3390/pathogens12091169 - 17 Sep 2023
Cited by 2 | Viewed by 1026
Abstract
Background: Given the diagnostic accuracy of HPV-DNA tests in terms of self-collected samples, in order to implement self-sampling in cervical screening programs, the standardization of the pre-analytical phase, including decisions concerning the choice of medium, the volume of elution, and storage conditions, are [...] Read more.
Background: Given the diagnostic accuracy of HPV-DNA tests in terms of self-collected samples, in order to implement self-sampling in cervical screening programs, the standardization of the pre-analytical phase, including decisions concerning the choice of medium, the volume of elution, and storage conditions, are necessary, in addition to understanding the potential factors involved in acceptability by women. On this basis, we carried out a cross-sectional study to assess (i) the stability of dry vaginal self-collected samples stored at room temperature for up to 4 weeks after elution in 2 mL of eNat® (Copan) medium, and (ii) the acceptability of self-collection in enrolled women. Methods: 185 women were enrolled in the LILT (Italian League Against Tumors) regional project. A self-sampling kit, including a dry FLOQSwab® (Copan), instructions for use, and a satisfaction questionnaire, were supplied for each woman and sent by mail to the laboratory. The HPV-DNA test was carried out using the Anyplex™ II HPV HR (Seegene) kit. To evaluate the specimen’s stability, 185 dry vaginal swabs were eluted in eNat®, a lyses-based molecular medium and tested for HPV detection at two different time points (<6 days and 1 month after elution). The Cohen’s Kappa coefficients and McNemar test were used to assess the agreement of HPV-DNA at different times. Results: We found high agreement in terms of HPV-DNA results among the samples tested at two different time points (Cohen K = 0.98; p < 0.0001). Moreover, most of the women found it easy to use self-collection devices and the pictorial instructions clear to understand. Approximately half of the enrolled women declared preferring self-sampling to clinician-collected methods. Conclusion: Our results display the high reliability and accuracy of HPV-DNA tests using dry vaginal self-collection FLOQSwabs® devices eluted in 2 mL of molecular medium. The analysis of the questionnaire showed a high acceptability of self-collection among women, although a high percentage preferred standard collection devices. Overall, our preliminary results support the adoption of self-collection in screening programs, even though further analyses should be performed to optimize and standardize protocols for HPV tests on self-samples, and educational campaigns are needed to adequately inform and increase responsiveness in a target population. Full article
(This article belongs to the Special Issue Advances in HPV Diagnostic Testing—Improving Access and Accuracy)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop