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Nanomaterials
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Nanoparticles in Regenerative Medicine
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Nanoparticles in Regenerative Medicine

A special issue of Nanomaterials (ISSN 2079-4991). This special issue belongs to the section "Biology and Medicines".

Deadline for manuscript submissions: closed (25 September 2022) | Viewed by 5944

Special Issue Editors

Dr. Sabine H. van Rijt

E-Mail Website
Guest Editor
Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands
Interests: biomaterials; nanoparticles; regenerative medicine
Prof. Dr. Aldo R. Boccaccini

grade E-Mail Website
Guest Editor
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
Interests: bioactive glasses; bioceramics; composite coatings; biofabrication; scaffolds; tissue engineering
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nanoparticles are popular materials for application in the regenerative medicine field as versatile platforms for biomolecule delivery, bioimaging and as functional parts in biomaterials. Specifically, nanoparticles can be used to deliver therapeutic agents, such as growth factors, ions, genes or other biomolecules to promote tissue or organ regeneration. These properties also make them interesting tools for stem-cell engineering purposes, to be able to control or predict stem-cell behavior in vivo. In addition to this, using nanoparticles as bioimaging tools to trace and characterize these cells in vivo to improve their efficacy, has received considerable attention in recent years. Additionally, within larger constructs, nanoparticles have made an important contribution. Biomaterials that can replace, regenerate or repair damaged cells or tissues are a popular strategy used within the field of regenerative medicine. The incorporation of nanoparticles within biomaterials has resulted in the development of nano-composite materials with improved mechanical and/or biological performance.

This Special Issue of Nanomaterials welcomes contributions on the development of nanoparticles for application in the regenerative medicine field, as outlined above. A special focus will be on studies dealing with nanoparticles that promote tissue and organ regeneration.

Dr. Sabine H. van Rijt
Prof. Dr. Aldo R. Boccaccini
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanoparticles
  • regenerative medicine
  • organ regeneration
  • tissue regeneration
  • drug delivery
  • biomolecule delivery
  • biomaterials
  • imaging
  • stem cells

Published Papers (3 papers)

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Research

16 pages, 2697 KiB  
Article
Gelatin Nanoparticles for Complexation and Enhanced Cellular Delivery of mRNA
by Lea Andrée, Rik Oude Egberink, Josephine Dodemont, Negar Hassani Besheli, Fang Yang, Roland Brock and Sander C. G. Leeuwenburgh
Nanomaterials 2022, 12(19), 3423; https://doi.org/10.3390/nano12193423 - 29 Sep 2022
Cited by 10 | Viewed by 2305
Abstract
Messenger RNA (mRNA) is increasingly gaining interest as a modality in vaccination and protein replacement therapy. In regenerative medicine, the mRNA-mediated expression of growth factors has shown promising results. In contrast to protein delivery, successful mRNA delivery requires a vector to induce cellular [...] Read more.
Messenger RNA (mRNA) is increasingly gaining interest as a modality in vaccination and protein replacement therapy. In regenerative medicine, the mRNA-mediated expression of growth factors has shown promising results. In contrast to protein delivery, successful mRNA delivery requires a vector to induce cellular uptake and subsequent endosomal escape to reach its end destination, the ribosome. Current non-viral vectors such as lipid- or polymer-based nanoparticles have been successfully used to express mRNA-encoded proteins. However, to advance the use of mRNA in regenerative medicine, it is required to assess the compatibility of mRNA with biomaterials that are typically applied in this field. Herein, we investigated the complexation, cellular uptake and maintenance of the integrity of mRNA complexed with gelatin nanoparticles (GNPs). To this end, GNPs with positive, neutral or negative surface charge were synthesized to assess their ability to bind and transport mRNA into cells. Positively charged GNPs exhibited the highest binding affinity and transported substantial amounts of mRNA into pre-osteoblastic cells, as assessed by confocal microscopy using fluorescently labeled mRNA. Furthermore, the GNP-bound mRNA remained stable. However, no expression of mRNA-encoded protein was detected, which is likely related to insufficient endosomal escape and/or mRNA release from the GNPs. Our results indicate that gelatin-based nanomaterials interact with mRNA in a charge-dependent manner and also mediate cellular uptake. These results create the basis for the incorporation of further functionality to yield endosomal release. Full article
(This article belongs to the Special Issue Nanoparticles in Regenerative Medicine)
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16 pages, 2728 KiB  
Article
Zn-Loaded and Calcium Phosphate-Coated Degradable Silica Nanoparticles Can Effectively Promote Osteogenesis in Human Mesenchymal Stem Cells
by Pichaporn Sutthavas, Matthias Schumacher, Kai Zheng, Pamela Habibović, Aldo Roberto Boccaccini and Sabine van Rijt
Nanomaterials 2022, 12(17), 2918; https://doi.org/10.3390/nano12172918 - 24 Aug 2022
Cited by 9 | Viewed by 2204
Abstract
Nanoparticles such as mesoporous bioactive glasses (MBGs) and mesoporous silica nanoparticles (MSN) are promising for use in bone regeneration applications due to their inherent bioactivity. Doping silica nanoparticles with bioinorganic ions could further enhance their biological performance. For example, zinc (Zn) is often [...] Read more.
Nanoparticles such as mesoporous bioactive glasses (MBGs) and mesoporous silica nanoparticles (MSN) are promising for use in bone regeneration applications due to their inherent bioactivity. Doping silica nanoparticles with bioinorganic ions could further enhance their biological performance. For example, zinc (Zn) is often used as an additive because it plays an important role in bone formation and development. Local delivery and dose control are important aspects of its therapeutic application. In this work, we investigated how Zn incorporation in MSN and MBG nanoparticles impacts their ability to promote human mesenchymal stem cell (hMSC) osteogenesis and mineralization in vitro. Zn ions were incorporated in three different ways; within the matrix, on the surface or in the mesopores. The nanoparticles were further coated with a calcium phosphate (CaP) layer to allow pH-responsive delivery of the ions. We demonstrate that the Zn incorporation amount and ion release profile affect the nanoparticle’s ability to stimulate osteogenesis in hMSCs. Specifically, we show that the nanoparticles that contain rapid Zn release profiles and a degradable silica matrix were most effective in inducing hMSC differentiation. Moreover, cells cultured in the presence of nanoparticle-containing media resulted in the highest induction of alkaline phosphate (ALP) activity, followed by culturing hMSC on nanoparticles immobilized on the surface as films. Exposure to nanoparticle-conditioned media did not increase ALP activity in hMSCs. In summary, Zn incorporation mode and nanoparticle application play an important role in determining the bioactivity of ion-doped silica nanoparticles. Full article
(This article belongs to the Special Issue Nanoparticles in Regenerative Medicine)
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14 pages, 2365 KiB  
Article
Pharmacological Dose-Effect Profiles of Various Concentrations of Humanised Primary Bile Acid in Encapsulated Cells
by Armin Mooranian, Melissa Jones, Daniel Walker, Corina Mihaela Ionescu, Susbin Raj Wagle, Bozica Kovacevic, Jacqueline Chester, Thomas Foster, Edan Johnston, Jafri Kuthubutheen, Daniel Brown, Marcus D. Atlas, Momir Mikov and Hani Al-Salami
Nanomaterials 2022, 12(4), 647; https://doi.org/10.3390/nano12040647 - 15 Feb 2022
Cited by 6 | Viewed by 1828
Abstract
Bile acids (BA)s are known surfactants and well-documented to play a major role in food digestion and absorption. Recently, potential endocrinological and formulation-stabilisation effects of BAs have been explored and their pharmacological effects on supporting cell survival and functions have gained wide interest. [...] Read more.
Bile acids (BA)s are known surfactants and well-documented to play a major role in food digestion and absorption. Recently, potential endocrinological and formulation-stabilisation effects of BAs have been explored and their pharmacological effects on supporting cell survival and functions have gained wide interest. Hence, this study aimed to explore the hyper-glycaemic dependent dose-effect of the BA chenodeoxycholic acid (CDCA) when encapsulated with pancreatic β-cells, allowing assessment of CDCA’s impacts when encapsulated. Four different concentrations of the BA were prepared, and viable cells were encapsulated and incubated for 2 days. Multiple analyses were carried out including confocal imaging, glucose-induced cellular mitochondrial viability indices, insulin production, inflammatory biomarker analyses and cellular bioenergetics measurements. There was a significant dose-effect with different concentrations of the BA, affecting cellular viability and antioxidant activities, cell functions and insulin release, inflammatory biomarkers, and cellular-bioenergetics at different oxidative stress levels. The results demonstrate that, when encapsulated, the BA CDCA exerts positive pharmacological effects at the cellular level, and such effects are concentration dependent. Full article
(This article belongs to the Special Issue Nanoparticles in Regenerative Medicine)
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