Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
4.6
Journals
Bioengineering
Special Issues
3D-Bioprinting in Bioengineering
share announcement

3D-Bioprinting in Bioengineering

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: closed (31 August 2023) | Viewed by 9806

Special Issue Editors

Prof. Dr. Raymund E. Horch

E-Mail Website
Guest Editor
Director, Laboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, Germany
Interests: microsurgery; flap surgery; hand surgery; reconstructive surgery; tissue engineering; biofabrication
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Aldo R. Boccaccini

grade E-Mail Website
Guest Editor
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
Interests: bioactive glasses; bioceramics; composite coatings; biofabrication; scaffolds; tissue engineering
Special Issues, Collections and Topics in MDPI journals
Dr. Małgorzata K. Włodarczyk-Biegun

E-Mail Website
Guest Editor
1. Polymer Science, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
2. Biotechnology Centre, Silesian University of Technology, Krzywousteg 8, 44-100 Gliwice, Poland
Interests: hydrogels; bioprinting; melt electrowriting; hard–soft tissue interfaces; gradients

Special Issue Information

Dear Colleagues,

The latest advances and technical developments have made 3D bio-printing increasingly available to the scientific community, and hence popular for numerous biomedical applications. While pre-bioprinting (essentially a 3D modelling step) may include scaffold generation with various resin or filament fibers, the usage of biologicalmaterial alone or in combination with cells deserves the further development of appropriate functional bioinks. These combinations comprise a whole new field within bioengineering that aims to further develop and optimize the techniques of 3D bioprinting itself, and to generate and perfect bioinks based on specifically designed cell-laden hydrogels. The vision is to enable the simultaneous processing of biomaterials and cells to 3D-print structures of sufficient biochemical and structural complexity that resemble and/or feature tissue properties. Given its enormous possibilities, 3D bioprinting has the power to become a disruptive technique in biomedical engineering, and is thus attracting massive interest from researchers from different disciplines worldwide.

We cordially invite all colleagues and researchers in this fascinating field to contribute to the present Special Issue, and look forward to receiving your contributions.

Prof. Dr. Raymund E. Horch
Prof. Dr. Aldo R. Boccaccini
Dr. Małgorzata K. Włodarczyk-Biegun
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomimicry and printing of hierarchical or gradient structures for TE
  • features and properties of bioinks
  • biomaterials for 3D printing
  • translational bioengineering and applications
  • biomechatronics
  • biomimetics
  • biomedical imaging and medical information systems
  • biological implants and regenerative medicine
  • biomodeling and simulation
  • vascularization of bioprinted items
  • bioprinting and cancer research models
  • cell–cell interactions for bioprinting
  • 4D bioprinting
  • functional bioinks
  • new printing approaches
  • merging different printing approaches

Published Papers (4 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

20 pages, 3466 KiB  
Article
Fabrication of Hydrogel-Based Composite Fibers and Computer Simulation of the Filler Dynamics in the Composite Flow
by Thomas Gruhn, Camilo Ortiz Monsalve, Claudia Müller, Susanne Heid, Aldo R. Boccaccini and Sahar Salehi
Bioengineering 2023, 10(4), 448; https://doi.org/10.3390/bioengineering10040448 - 06 Apr 2023
Cited by 2 | Viewed by 1832
Abstract
Fibrous structures with anisotropic fillers as composites have found increasing interest in the field of biofabrication since they can mimic the extracellular matrix of anisotropic tissues such as skeletal muscle or nerve tissue. In the present work, the inclusion of anisotropic fillers in [...] Read more.
Fibrous structures with anisotropic fillers as composites have found increasing interest in the field of biofabrication since they can mimic the extracellular matrix of anisotropic tissues such as skeletal muscle or nerve tissue. In the present work, the inclusion of anisotropic fillers in hydrogel-based filaments with an interpenetrating polymeric network (IPN) was evaluated and the dynamics of such fillers in the composite flow were analyzed using computational simulations. In the experimental part, microfabricated rods (200 and 400 μm length, 50 μm width) were used as anisotropic fillers in extrusion of composite filaments using two techniques of wet spinning and 3D printing. Hydrogels such as oxidized alginate (ADA) and methacrylated gelatin (GelMA) were used as matrices. In the computational simulation, a combination of computational fluid dynamics and coarse-grained molecular dynamics was used to study the dynamics of rod-like fillers in the flow field of a syringe. It showed that, during the extrusion process, microrods are far from being well aligned. Instead, many of them tumble on their way through the needle leading to a random orientation in the fiber which was confirmed experimentally. Full article
(This article belongs to the Special Issue 3D-Bioprinting in Bioengineering)
►▼ Show Figures

Figure 1

18 pages, 5117 KiB  
Article
Indirect 3D Bioprinting of a Robust Trilobular Hepatic Construct with Decellularized Liver Matrix Hydrogel
by Vamakshi Khati, Johannes Artturi Turkki, Harisha Ramachandraiah, Falguni Pati, Giulia Gaudenzi and Aman Russom
Bioengineering 2022, 9(11), 603; https://doi.org/10.3390/bioengineering9110603 - 22 Oct 2022
Cited by 9 | Viewed by 2325
Abstract
The liver exhibits complex geometrical morphologies of hepatic cells arranged in a hexagonal lobule with an extracellular matrix (ECM) organized in a specific pattern on a multi-scale level. Previous studies have utilized 3D bioprinting and microfluidic perfusion systems with various biomaterials to develop [...] Read more.
The liver exhibits complex geometrical morphologies of hepatic cells arranged in a hexagonal lobule with an extracellular matrix (ECM) organized in a specific pattern on a multi-scale level. Previous studies have utilized 3D bioprinting and microfluidic perfusion systems with various biomaterials to develop lobule-like constructs. However, they all lack anatomical relevance with weak control over the size and shape of the fabricated structures. Moreover, most biomaterials lack liver-specific ECM components partially or entirely, which might limit their biomimetic mechanical properties and biological functions. Here, we report 3D bioprinting of a sacrificial PVA framework to impart its trilobular hepatic structure to the decellularized liver extracellular matrix (dLM) hydrogel with polyethylene glycol-based crosslinker and tyrosinase to fabricate a robust multi-scale 3D liver construct. The 3D trilobular construct exhibits higher crosslinking, viscosity (182.7 ± 1.6 Pa·s), and storage modulus (2554 ± 82.1 Pa) than non-crosslinked dLM. The co-culture of HepG2 liver cells and NIH 3T3 fibroblast cells exhibited the influence of fibroblasts on liver-specific activity over time (7 days) to show higher viability (90–91.5%), albumin secretion, and increasing activity of four liver-specific genes as compared to the HepG2 monoculture. This technique offers high lumen patency for the perfusion of media to fabricate a densely populated scaled-up liver model, which can also be extended to other tissue types with different biomaterials and multiple cells to support the creation of a large functional complex tissue. Full article
(This article belongs to the Special Issue 3D-Bioprinting in Bioengineering)
►▼ Show Figures

Graphical abstract

Review

Jump to: Research

18 pages, 1008 KiB  
Review
Three-Dimensional Bioprinting in Soft Tissue Engineering for Plastic and Reconstructive Surgery
by Astrid Bülow, Benedikt Schäfer and Justus P. Beier
Bioengineering 2023, 10(10), 1232; https://doi.org/10.3390/bioengineering10101232 - 21 Oct 2023
Cited by 1 | Viewed by 1558
Abstract
Skeletal muscle tissue engineering (TE) and adipose tissue engineering have undergone significant progress in recent years. This review focuses on the key findings in these areas, particularly highlighting the integration of 3D bioprinting techniques to overcome challenges and enhance tissue regeneration. In skeletal [...] Read more.
Skeletal muscle tissue engineering (TE) and adipose tissue engineering have undergone significant progress in recent years. This review focuses on the key findings in these areas, particularly highlighting the integration of 3D bioprinting techniques to overcome challenges and enhance tissue regeneration. In skeletal muscle TE, 3D bioprinting enables the precise replication of muscle architecture. This addresses the need for the parallel alignment of cells and proper innervation. Satellite cells (SCs) and mesenchymal stem cells (MSCs) have been utilized, along with co-cultivation strategies for vascularization and innervation. Therefore, various printing methods and materials, including decellularized extracellular matrix (dECM), have been explored. Similarly, in adipose tissue engineering, 3D bioprinting has been employed to overcome the challenge of vascularization; addressing this challenge is vital for graft survival. Decellularized adipose tissue and biomimetic scaffolds have been used as biological inks, along with adipose-derived stem cells (ADSCs), to enhance graft survival. The integration of dECM and alginate bioinks has demonstrated improved adipocyte maturation and differentiation. These findings highlight the potential of 3D bioprinting techniques in skeletal muscle and adipose tissue engineering. By integrating specific cell types, biomaterials, and printing methods, significant progress has been made in tissue regeneration. However, challenges such as fabricating larger constructs, translating findings to human models, and obtaining regulatory approvals for cellular therapies remain to be addressed. Nonetheless, these advancements underscore the transformative impact of 3D bioprinting in tissue engineering research and its potential for future clinical applications. Full article
(This article belongs to the Special Issue 3D-Bioprinting in Bioengineering)
►▼ Show Figures

Figure 1

19 pages, 6178 KiB  
Review
3D Bioprinting for Vascularization
by Amatullah Mir, Eugenia Lee, Wesley Shih, Sarah Koljaka, Anya Wang, Caitlin Jorgensen, Riley Hurr, Amartya Dave, Krupa Sudheendra and Narutoshi Hibino
Bioengineering 2023, 10(5), 606; https://doi.org/10.3390/bioengineering10050606 - 18 May 2023
Cited by 11 | Viewed by 3174
Abstract
In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to [...] Read more.
In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to develop successful 3D tissue constructs for clinical applications. However, current research in 3D bioprinting model development lacks diverse methods of successful vascularization as a result of issues with scaling, size, and variations in printing method. This study analyzes the methods of printing, bioinks used, and analysis techniques in 3D bioprinting for vascularization. These methods are discussed and evaluated to determine the most optimal strategies of 3D bioprinting for successful vascularization. Integrating stem and endothelial cells in prints, selecting the type of bioink according to its physical properties, and choosing a printing method according to physical properties of the desired printed tissue are steps that will aid in the successful development of a bioprinted tissue and its vascularization. Full article
(This article belongs to the Special Issue 3D-Bioprinting in Bioengineering)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-4
Back to TopTop