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Microorganisms
Special Issues
Esther Lederberg’s 100th Anniversary: Microbial Genetics and Bacteriophages
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Esther Lederberg’s 100th Anniversary: Microbial Genetics and Bacteriophages

A special issue of Microorganisms (ISSN 2076-2607).

Deadline for manuscript submissions: closed (30 December 2023) | Viewed by 2159

Special Issue Editor

Prof. Dr. Grzegorz Wegrzyn

E-Mail Website
Guest Editor
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland
Interests: gene expression regulation; DNA replication; bacteriophages; plasmids; human genetic diseases; neurodegeneration
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Esther Miriam Zimmer Lederberg (1922–2006) was an American microbiologist and a major pioneer in the field of bacterial genetics and the study of bacteriophages.

She completed her master’s degree at Stanford University and then moved to the University of Wisconsin-Madison where she completed her doctorate (1950). As part of her outstanding research career, she discovered the lambda phage, a bacterial virus which is a fundamental tool for today’s studies on gene regulation and genetic recombination. She also invented the replica plating technique, which is widely used to isolate and analyze bacterial mutants and to monitor antibiotic resistance.

Her remarkable findings laid the groundwork for demonstrating how phages can transfer genes between bacteria, and were crucial to advancing the understanding of key aspects such as how genes are regulated and the process of DNA recombination.

To commemorate the 100th anniversary of the birth of Dr. Lederberg and to recognize her outstanding career, in this Special Issue we provide a platform for experts in the fields of bacterial genetics and the study of bacteriophages to share their most recent advances in these areas. We welcome research articles, comprehensive reviews, communications, and perspectives.

Prof. Dr. Grzegorz Wegrzyn
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial molecular biology
  • microbial physiology
  • bacteriophages
  • plasmids
  • gene expression regulation
  • genetic recombination in bacteria
  • DNA replication control in bacterial cells
  • mutagenesis and DNA repair in bacteria

Published Papers (1 paper)

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Research

14 pages, 3533 KiB  
Article
Response Surface Methodology Application for Bacteriophage–Antibiotic Antibiofilm Activity Optimization
by Bartłomiej Grygorcewicz, Marta Gliźniewicz, Patrycja Olszewska, Dominika Miłek, Artur Czajkowski, Natalia Serwin, Elżbieta Cecerska-Heryć and Rafał Rakoczy
Microorganisms 2023, 11(9), 2352; https://doi.org/10.3390/microorganisms11092352 - 20 Sep 2023
Cited by 3 | Viewed by 1464
Abstract
Phage–antibiotic combination-based protocols are presently under heightened investigation. This paradigm extends to engagements with bacterial biofilms, necessitating novel computational approaches to comprehensively characterize and optimize the outcomes achievable via these combinations. This study aimed to explore the Response Surface Methodology (RSM) in optimizing [...] Read more.
Phage–antibiotic combination-based protocols are presently under heightened investigation. This paradigm extends to engagements with bacterial biofilms, necessitating novel computational approaches to comprehensively characterize and optimize the outcomes achievable via these combinations. This study aimed to explore the Response Surface Methodology (RSM) in optimizing the antibiofilm activity of bacteriophage–antibiotic combinations. We employ a combination of antibiotics (gentamicin, meropenem, amikacin, ceftazidime, fosfomycin, imipenem, and colistin) alongside the bacteriophage vB_AbaP_AGC01 to combat Acinetobacter baumannii biofilm. Based on the conducted biofilm challenge assays analyzed using the RSM, the optimal points of antibiofilm activity efficacy were effectively selected by applying this methodology, enabling the quantifiable mathematical representations. Subsequent optimization showed the synergistic potential of the anti-biofilm that arises when antibiotics are judiciously combined with the AGC01 bacteriophage, reducing biofilm biomass by up to 80% depending on the antibiotic used. The data suggest that the phage–imipenem combination demonstrates the highest efficacy, with an 88.74% reduction. Notably, the lower concentrations characterized by a high maximum reduction in biofilm biomass were observed in the phage–amikacin combination at cA = 0.00195 and cP = 0.38 as the option that required minimum resources. It is worth noting that only gentamicin antagonism between the phage and the antibiotic was detected. Full article
(This article belongs to the Special Issue Esther Lederberg’s 100th Anniversary: Microbial Genetics and Bacteriophages)
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