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Membranes
Special Issues
Application of Advanced Membrane Materials in Bioseparation
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Application of Advanced Membrane Materials in Bioseparation

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Membrane Applications".

Deadline for manuscript submissions: closed (30 October 2023) | Viewed by 6048

Special Issue Editors

Dr. Riyadh Al-Attabi

E-Mail Website
Guest Editor
Institute for Frontier Materials, Deakin University, Melbourne, VIC, Australia
Interests: electrospinning; membranes; nanotechnology; materials characterization
Dr. Xing Yang

E-Mail Website
Guest Editor
Department of Chemical Engineering, 3000 Leuven, Belgium
Interests: process intensification and integration for designing energy-efficient hybrid membrane systems; transport modeling and process simulation in the areas of resource recovery; bioseparation and chemical sensing
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Lingxue Kong

E-Mail Website
Guest Editor
Institute for Frontier Materials, Deakin University, Waurn Ponds, VIC 3216, Australia
Interests: nanostructured membranes; porous materials; membrane characterization
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue on “Application of Advanced Membrane Materials in Bioseparation” will critically discuss membrane technology for bioseparation and purification in terms of recent advancements. Topics will range from innovations in membrane manufacturing, membrane properties, and surface functionality to the influence of operating parameters on the performance of membrane materials. Membrane bioseparation has been widely used as an effective and reliable method for the separation and purification of bioproducts such as enzymes, proteins, peptides, and nucleic acids. The bases of separation used in the membrane bioseparation process include size, diffusivity, polarity, electrostatic charge, and volatility. Membrane bioseparation technology must combine high selectivity and throughput (productivity). This Special Issue will focus on the connection between advanced membrane materials and their applications in membrane bioseparation. We invite researchers to contribute their original and high-quality research papers and/or review articles, which will inspire advances in the design of advanced functional membranes for bioseparation. The scope includes, but is not limited to:

  • Membrane chromatography;
  • Biocatalytic membranes;
  • Nonwoven membranes for bioseparation;
  • Ion exchange membrane chromatography;
  • Affinity nanofibrous chromatographic membranes;
  • Downstream bioprocessing;
  • Protein purification;
  • Flow dynamics;
  • Process modelling for membrane chromatography;
  • CFD modelling;
  • Molecular simulations;
  • Natural polymeric adsorbents;
  • Inorganic fibrous adsorbents;
  • Synthetic polymeric adsorbents;
  • Fiber-based protein adsorbents;
  • Surface functionalization;
  • Composite nanofiber membranes.

Dr. Riyadh Al-Attabi
Dr. Xing Yang
Prof. Dr. Lingxue Kong
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Keywords

  • membrane chromatography
  • biocatalytic membranes
  • nonwoven membranes for bioseparation
  • ion exchange membrane chromatography
  • affinity nanofibrous chromatographic membranes
  • downstream bioprocessing
  • protein purification
  • flow dynamics
  • process modelling for membrane chromatography
  • CFD modelling
  • molecular simulations
  • natural polymeric adsorbents
  • inorganic fibrous adsorbents
  • synthetic polymeric adsorbents
  • fiber-based protein adsorbents
  • surface functionalization
  • composite nanofiber membranes

Published Papers (3 papers)

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Research

17 pages, 3101 KiB  
Article
Development of a New Affinity Gold Polymer Membrane with Immobilized Protein A
by Tobias Steegmüller, Tim Kratky, Lena Gollwitzer, Sebastian Patrick Schwaminger and Sonja Berensmeier
Membranes 2024, 14(2), 31; https://doi.org/10.3390/membranes14020031 - 24 Jan 2024
Viewed by 1367
Abstract
New and highly selective stationary phases for affinity membrane chromatography have the potential to significantly enhance the efficiency and specificity of therapeutic protein purification by reduced mass transfer limitations. This work developed and compared different immobilization strategies for recombinant Protein A ligands to [...] Read more.
New and highly selective stationary phases for affinity membrane chromatography have the potential to significantly enhance the efficiency and specificity of therapeutic protein purification by reduced mass transfer limitations. This work developed and compared different immobilization strategies for recombinant Protein A ligands to a gold-sputtered polymer membrane for antibody separation in terms of functionalization and immobilization success, protein load, and stability. Successful, functionalization was validated via X-ray photoelectron spectroscopy (XPS). Here, a recombinant Protein A ligand was coupled by N-hydroxysuccinimide (NHS)/N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) chemistry to carboxy-functionalized, gold-sputtered membranes. We achieved a binding capacity of up to 104 ± 17 mg of the protein ligand per gram of the gold-sputtered membrane. The developed membranes were able to successfully capture and release the monoclonal antibody (mAb) Trastuzumab, as well as antibodies from fresh frozen human blood plasma in both static and dynamic setups. Therefore, they demonstrated successful functionalization and immobilization strategies. The antibody load was tested using bicinchoninic acid (BCA), ultraviolet-visible spectroscopy (UV-vis) measurements, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The outcome is a fully functional affinity membrane that can be implemented in a variety of different antibody purification processes, eliminating the need for creating individualized strategies for modifying the surface to suit different substrates or conditions. Full article
(This article belongs to the Special Issue Application of Advanced Membrane Materials in Bioseparation)
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17 pages, 2921 KiB  
Article
pH-Dependent Adsorption of Human Serum Albumin Protein on a Polystyrene-Block–Poly(acrylic acid)-Coated PVDF Membrane
by Charaf-Eddine Merzougui, Pierre Aimar, Patrice Bacchin and Christel Causserand
Membranes 2023, 13(12), 886; https://doi.org/10.3390/membranes13120886 - 22 Nov 2023
Viewed by 1577
Abstract
This study reports the investigation of human serum albumin (HSA) adsorption on a poy-styrene-block–poly(acrylic acid) (PS-b-PAA)-coated PVDF membrane, which is a potential smart material for biomedical applications. First, copolymer coating on the membrane surface was successfully performed, due to the hydrophobic interaction of [...] Read more.
This study reports the investigation of human serum albumin (HSA) adsorption on a poy-styrene-block–poly(acrylic acid) (PS-b-PAA)-coated PVDF membrane, which is a potential smart material for biomedical applications. First, copolymer coating on the membrane surface was successfully performed, due to the hydrophobic interaction of the PS anchoring group with the PVDF membrane. This was confirmed by Fourier transform infrared spectroscopy (FTIR) characterization of the membrane. Then, HSA adsorption onto the coated membrane was assessed and was proved to be strongly dependent on the pH of the protein solution. Indeed, both FTIR mapping and mass balance calculation using UV–visible spectroscopy displayed a greater HSA adsorption on the membrane at pH 5, even though it still took place at higher pH, but to a lower extent. Afterwards, an ionic strength influence study evinced the role of electrostatic interactions between HSA and the PAA layer on HSA adsorption. Dead-end filtration of HSA through the coated membrane confirmed the pH dependence of HSA adsorption on the coated membrane. Full article
(This article belongs to the Special Issue Application of Advanced Membrane Materials in Bioseparation)
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15 pages, 1646 KiB  
Article
Scalability of Sartobind® Rapid A Membrane for High Productivity Monoclonal Antibody Capture
by Sabrina Yang, Ryszard Braczkowski, Shih-Hsun Chen, Ricarda Busse, Yudhi Li, Louis Fabri and Innocent Berbelle Bekard
Membranes 2023, 13(10), 815; https://doi.org/10.3390/membranes13100815 - 27 Sep 2023
Cited by 2 | Viewed by 2177
Abstract
Improved upstream titres in therapeutic monoclonal antibody (mAb) production have shifted capacity constraints to the downstream process. The consideration of membrane-based chromatographic devices as a debottlenecking option is gaining increasing attention with the recent introduction of high-capacity bind and elute membranes. We have [...] Read more.
Improved upstream titres in therapeutic monoclonal antibody (mAb) production have shifted capacity constraints to the downstream process. The consideration of membrane-based chromatographic devices as a debottlenecking option is gaining increasing attention with the recent introduction of high-capacity bind and elute membranes. We have evaluated the performance and scalability of the Sartobind® Rapid A affinity membrane (1 mL) for high-productivity mAb capture. For scalability assessment, a 75 mL prototype device was used to process 100 L of clarified cell culture harvest (CH) on a novel multi-use rapid cycling chromatography system (MU-RCC). MabSelect™ PrismA (4.7 mL) was used as a benchmark comparator for Protein A (ProtA) resin studies. Results show that in addition to a productivity gain of >10×, process and product quality attributes were either improved or comparable to the benchmark. Concentrations of eluate pools were 7.5× less than that of the benchmark, with the comparatively higher bulk volume likely to cause handling challenges at process scale. The MU-RCC system is capable of membrane operation at pilot scale with comparable product quality profile to the 1 mL device. The Sartobind® Rapid A membrane is a scalable alternative to conventional ProtA resin chromatography for the isolation and purification of mAbs from harvested cell culture media. Full article
(This article belongs to the Special Issue Application of Advanced Membrane Materials in Bioseparation)
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